• Reproductive and Developmental Biology
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• 제34권3호
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• pp.229-233
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• 2010

Pluripotency and self-renewal capacity of human embryonic stem cells (hESCs) are retained by hESCs related genes as OCT4, SOX2 and NANOG. These genes are shown high expression level in diverse cancer cells and have potential role in the carcinogenesis. On the contrary to this, several genes which are up-regulated in the differentiated hESCs are involved to suppress the carcinogenesis or proliferation of cells. We discovered several genes in immortalized lung fibroblast (WI-38 VA13) by suppression subtractive hybridization. Among them, we focused chromosome 6 open reading frame 62 (C6orf62) which is uncharacterized, mapped to 6p22.3 and generated to Hepatitis B virus X-transactivated proteins (HBVx-transactivated proteins, XTP). Aim of this study was to characterize C6orf62 through analyzing of expression pattern in various cell lines. Expression of C6orf62 was significantly upregulated in diverse normal cell lines than cancer cell lines. And C6orf62 was up-regulated in differentiated hESCs (endothelial cells, neural cells) compared to those of undifferentiated hESCs. Also, C6orf62 in WI-38 cells was highly up-regulated during G1/S transition of the cell cycle. Taken together, C6orf62 is shown expression pattern similar to differentiated hESCs-associated genes which down-regulated in cancer cells. Therefore, we assume that C6orf62 may participate to suppress the proliferation and to induce differentiation through regulating the cell cycle.

• Asian Pacific Journal of Cancer Prevention
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• 제16권7호
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• pp.2939-2946
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• 2015

Many of the anti-cancer agents currently used have an origin in natural sources including plants. Aloe vera is one such plant being studied extensively for its diverse health benefits, including cancer prevention. In this study, the cytotoxic potential of Aloe vera crude extract (ACE) alone or in combination with cisplatin in human breast (MCF-7) and cervical (HeLa) cancer cells was studied by cell viability assay, nuclear morphological examination and cell cycle analysis. Effects were correlated with modulation of expression of genes involved in cell cycle regulation, apoptosis and drug metabolism by RT-PCR. Exposure of cells to ACE resulted in considerable loss of cell viability in a dose- and time-dependent fashion, which was found to be mediated by through the apoptotic pathway as evidenced by changes in the nuclear morphology and the distribution of cells in the different phases of the cell cycle. Interestingly, ACE did not have any significant cytotoxicity towards normal cells, thus placing it in the category of safe chemopreventive agent. Further, the effects were correlated with the downregulation of cyclin D1, CYP 1A1, CYP 1A2 and increased expression of bax and p21 in MCF-7 and HeLa cells. In addition, low dose combination of ACE and cisplatin showed a combination index less than 1, indicating synergistic growth inhibition compared to the agents applied individually. In conclusion, these results signify that Aloe vera may be an effective anti-neoplastic agent to inhibit cancer cell growth and increase the therapeutic efficacy of conventional drugs like cispolatin. Thus promoting the development of plant-derived therapeutic agents appears warranted for novel cancer treatment strategies.

• Archives of Pharmacal Research
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• 제29권11호
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• pp.1032-1041
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• 2006

Extracellular adenosine 5'-triphosphate (ATP) affects the function of many tissues and cells. To confirm the biological activity of ATP on human myeloid leukemic cells, F-36P and HL-60, cells were treated with a variety of concentrations of ATP. The stimulation with extracellular ATP induced the arrest of cell proliferation and cell death. from the analysis of Annexin-V staining and caspase activity by flow cytometry. The Annexin-V positive cells in both cell lines were dramatically increased following ATP stimulation. The expression of P2 purinergic receptor genes was confirmed, such as P2X1, P2X4, P2X5, P2X7 and P2Y1, P2Y2, P2Y4, P2Y5, P2Y6, P2Y11 in both leukemic cell lines. Interestingly, ATP induced intracellular calcium flux in HL-60 cells but not in F-36P cells, as determined by Fluo-3 AM staining. Cell cycle analysis revealed that ATP treatment arrested both F-36P and HL-60 cells at G1/G0. Taken together, these data showed that extracellular ATP via P2 receptor genes was involved in the cell proliferation and survival in human myeloid leukemic cells, HL-60 and F-36P cells by the induction of apoptosis and control of cell cycle. Our data suggest that treatment with extracellular nucleotides may be a novel and powerful therapeutic avenue for myeloid leukemic disease.

• 가정과삶의질연구
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• 제34권3호
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• pp.147-163
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• 2016

Multicultural Family Support Centers (MFSC) have provided five program areas including family, gender equality, human rights, social integration, and counseling since 2015. It is important to examine the MFSC staff's perceptions of the family programs and program needs for multicultural families to improve the effectiveness of these programs. In this study, we aim to explore the staff's perceptions of the family programs and to assess multicultural family program needs based on the family life cycle. A total of 130 MFSC staff were recruited through 128 MFSCs across Korea. Descriptive statistics were conducted for data analysis. Our findings revealed that MFSC staff have increasingly recognized that their target audiences are not only marriage immigrants and their families but also foreign workers' families and families from North Korea. In addition, the MFSC staff identified the importance of family programs instead of only programs for individual family members and multicultural families' different program needs based on their family life cycle. Contrary to the positive perception of the family programs, they described challenges of the family programs including a shortage of funds, difficulty recruiting family member combinations (e.g., couples, parent-child) for family programs, a lack of a program manual, and a heavy workload. This study provides insights into the family programs including their development and delivery.

• Asian Pacific Journal of Cancer Prevention
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• 제15권15호
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• pp.6437-6441
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• 2014

Background: Oridonin isolated from Rabdosia rubescens, a plant used to treat cancer in Chinese folk medicine, is one of the most important antitumor active ingredients. Previous studies have shown that oridonin has antitumor activities in vivo and in vitro, but little is known about cell cycle effects of oridonin in gastric cancer. Materials and Methods: MTT assay was adopted to detect the proliferation inhibition of SGC-7901 cells, the cell cycle was assessed by flow cytometry and protein expression by Western blotting. Results: Oridonin could inhibit SGC-7901 cell proliferation, the $IC_{50}$ being $15.6{\mu}M$, and blocked SGC-7901 cell cycling in the $G_2/M$ phase. The agent also decreased the protein expression of cyclinB1 and CDK1. Conclusions: Oridonin may inhibit SGC-7901 growth and block the cells in the $G_2/M$ phase by decreasing Cdk1 and cyclinB1 proteins.

• 지능정보연구
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• 제15권4호
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• pp.37-51
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• 2009

서비스 인적자원 운용의 효율성 제고와 부품 또는 시설 할당의 적정성 향상을 위해 서비스센터를 통해 접수되는 서비스 요청 건수를 보다 정확하게 예측하고자 하는 필요성이 제조업을 중심으로 증가하고 있다. 본 연구에서는 제품의 특성을 반영하여 제품수명주기 별로 제품들을 군집화하고 군집 별로 적절한 예측모형을 구축한 후 예측 값을 통합하는 개별예측방식을 LCD 모니터 제조사의 사례를 통해 제시한다. 또한 예측 결과를 총량방식 및 기존에 기업이 사용하고 있는 방식과 비교. 평가하여 우수성을 증명함으로써 제품이나 산업의 특성을 반영한 맞춤형 수요예측 기법 도입의 필요성을 부각하고, 그에 따른 이론적, 실무적 가이드라인을 제공한다.

• Environmental Engineering Research
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• 제21권3호
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• pp.284-290
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• 2016

Life cycle assessment (LCA) methodology can be used to assess impacts on the environment that might be generated during treatment of wastewater and sludge treatment. In this work, LCA methodology was suggested to evaluate monthly environmental impact of wastewater treatment plants (WWTPs). Two field scale WWTPs, A2/O process and conventional activated sludge process (CAS), were selected as target plants and the operational data were collected from those plants. As the function units, the unit volume of treated wastewater of $1m^3$ and 1 kg T-N eq. removed were selected. The environmental effect of target WWTPs operation were assessed as impact categories such as global warming potential, eutrophication potential, and so on. From monthly profiles of each index, it was shown that the environmental impact of WWTPs has seasonal patterns influenced by the influent flow rate variation causing higher impacts in winter than summer. This is due to the fact that there were no significant increase in the electricity consumption and chemical usage during the summer while the treated volume of wastewater was increased.

• 청정기술
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• 제23권4호
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• pp.388-392
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• 2017

본 연구에서는 통기성 필름의 제조 공정에 대한 환경영향 특성을 분석하기 위해 전과정평가를 수행하였다. 특성화와 정규화 결과, 영향범주 별 환경영향은 지구온난화가 약 97%. 인간독성 2%의 기여도를 나타났다. 주요 기여도는 투입물인 고밀도 폴리에틸렌(HDPE)과 폴리프로필렌(PP) 그리고 에너지인 전력으로 나타났다. 세부 공정에서는 혼합단계(Mixing)가 57%, 부착단계(Lamination) 29% 압출단계(Extruder) 10% 순으로 높은 환경영향 기여도를 보였다. 환경 영향을 개선하기 위해서는 전력 사용량을 감소시키거나 원재료인 HDPE나 PP를 제외한 수율 향상을 고려한 새로운 공정 시스템 설계가 필요하다.

• 한국구조물진단유지관리공학회 논문집
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• 제6권4호
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• pp.189-199
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• 2002

This study is intended to propose a systematic procedure for the development of the conditional assessment based on the safety of structures and the cost effective performance criteria for designing and upgrading of bridge structures. As a result, a set of cost function models for a life cycle cost analysis of bridge structures is proposed and thus the expected total life cycle costs (ETLCC) including initial (design, testing and construction) costs and direct/indirect damage costs considering repair and replacement costs, human losses and property damage costs, road user costs, and indirect regional economic losses costs. Also, the optimum safety indices are presented based on the expected total cost minimization function using only three parameters of the failure cost to the initial cost (${\tau}$), the extent of increased initial cost by improvement of safety (${\nu}$) and the order of an initial cost function (n). Through the enough numerical invetigations, we can positively conclude that the proposed optimum design procedure for bridge structures based on the ETLCC will lead to more rational, economical and safer design.

• 청정기술
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• 제24권1호
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• pp.9-14
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• 2018

본 연구에서는 TiN-Zr 수소분리막의 제조 공정에 대한 환경 영향 특성을 분석하기 위해 물질전과정평가를 수행하였다. Material Life Cycle Assessment (MLCA)의 소프트웨어로는 Gabi를 사용하였다. 이를 통하여 각 공정에서 미치는 영향과 특성화 별 환경영향평가를 수행하였다. 졸겔법에 의해 전구체 TiN을 합성하고 볼밀법을 이용하여 지르코늄을 코팅하였다. 이를 CIP, HPS에 의해 디스크 형으로 제작하였고 주사전자현미경(scanning electron microscopy, SEM), 에너지분산형 분광분석법(energy dispersive X-ray spectroscopy, EDS), X-선 회절분석기(X-ray diffraction, XRD), 열중량분석(thermo gravimetry/differential thermal analysis, TG/DTA), 비표면적분석(Brunauer, Emmett, Teller, BET) 및 가스 크로마토그래프 시스템(gas chromatograph system, GP)을 이용하여 분리막의 야금학적, 물리학적, 열역학적 특성을 분석하였다. 또한, 물질전과정평가를 위해 수행한 특성화와 정규화 결과, 영향범주 별 환경영향은 해양 생태 독성이 94%, 수계 생태 독성 2%, 인간독성 2%의 기여도를 보였다. 아울러, 제조공정 중 전기 사용이 생태계 영향에 큰 영향을 미친다는 것을 알 수 있었다. 물질 전 과정 평가는 Eco-Indicator '99 (EI99)와 CML 2001 방법론을 기반으로 분석하였다.