• Journal of Life Science
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• v.17 no.12
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• pp.1641-1648
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• 2007

Yippee-like proteins, which have been identified as the homolog of Drosophila yippee protein containing a zinc-finger domain, are known to be highly conserved among eukaryotes. However, their functional roles are still poorly understood. Recently we initiated ordered differential display (ODD)-polymerase chain reaction (PCR) to isolate genes of which expressions are altered following activation of human T cells. On the ODD-PCR image, one PCR-product detected in unstimulated T cells was not detectable at the time when the activated T cells traversed near $G_1/S$ boundary following activation by immobilized anti-CD3. Cloning and nucleotide sequence analysis revealed that the PCR-product was yippee-like 5 (YPEL5) gene, which was known as a human homolog of the Drosophila yippee gene. Northern blot analysis confirmed the amount of ${\sim}2.2$ kb YPEL5 mRNA expression detectable in unstimulated T cells was sustained until 1.5 hr after activation and then rapidly declined to undetectable level by 5 hr. Ectopic expression of YPEL5 gene in human cervix epitheloid carcinoma HeLa cells caused a significant reduction in cell proliferation to the level of 47% of the control. Expression of GFP-YPEL5 fusion protein in HeLa cells showed its nuclear localization. These results demonstrated that the expression level of human YPEL5 mRNA was negatively regulated in the early stage of T cell activation, and suggested that YPEL5 might exert an inhibitory effect on the cell proliferation as a nuclear protein.

• The Korean Journal of Malacology
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• v.19 no.2
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• pp.117-124
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• 2003

Reproductive cycle and sex ratio of the marsh clam Corbicula japonica, a oviparous clam, were investigated monthly by histological observation. Samples were collected in brackish water of Namdae stream on the east coast of Korea from November 2000 to October 2001. It was able to devide the reproductive cycle of this species into five successive stages; early active (April to June), late active (May to June), ripe (June to August), partially spawned (June to September), spent (September to January) and resting stage (February to April). The spawning period was from July to September, and the main spawning occurred between August and September when seawater temperatures reached above 26$^{\circ}C$. Mature eggs of Corbicula japonica were 60-70 ${\mu}m$ in diameter. The sex ratio of individuals over 10.1 mm in shell length was about 1:1 (x$^2$ = 1.22, p > 0.05).

• Journal of Life Science
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• v.21 no.12
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• pp.1666-1677
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• 2011

The regulation of gene expression plays an important role in cell cycle controls. In this study, a novel gene, the $mas1^+$($\underline{mi}$tosis $\underline{as}$sociated protein) gene, a homolog of human CIP29/Hcc1, was isolated and characterized from fission yeast Schizosaccharomyces pombe (S. pombe) using a gene-specific polymerase chain reaction. The isolated gene contained a complete open reading frame capable of encoding 245 amino acid residues with a typical promoter, as judged by nucleotide sequence analysis. It was also found that a PCB ($\underline{p}$ombe cell $\underline{c}$ycle $\underline{b}$ox) is located in the promoter region, which controls M-$G_1$ specific transcription in S. pombe. The quantitative analysis of the $mas1^+$ transcript against $adh1^+$ showed that the pattern of expression is similar to that of the septation index. Cytokinesis of mas1 mutant was greatly delayed at $25^{\circ}C$ and $36^{\circ}C$, and a large number of multi-septate cells were produced. The mas1 mutant had 2C, 4C and 6C DNA contents, as determined by FACS analysis. In addition, the number of multi-septate cells significantly increased. When cells were cultured in nitrogen starvation medium to increase proliferation, the abnormal phenotypes of mas1 mutant dramatically increased. These phenotypes could be rescued by an overexpression of the $mas1^+$ gene. The mas1 protein localized in the nuclei of S. pombe and human HeLa cells, as evidenced by Mas1-EGFP signals. The abnormal growth pattern and the morphology of mas1 mutant were complemented by a plasmid carrying human CIP29/Hcc-1cDNA. In addition, CIP29 /Hcc-1 transcript level increased in active cell proliferation stages in the developing mouse embryos. These results indicate that the $mas1^+$ ishomologous to the human CIP29/Hcc1 gene and is involved in cytokinesis and cell shape control.

• Journal of Microbiology and Biotechnology
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• v.10 no.3
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• pp.429-433
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• 2000

Giardia lamblia, a human pathogen causing outbreaks of diarrhea, recently became a focus of great concerns in the fields of both medical and environmental microbioloty. To develop the experimental tools to study giardiasis, encystation, one of the major processes in its life cycle, was reconstituted by inducing an axenic culture of a flagellated form of G. lamblia into a cyst from under high concentration of bile and alkaline pH condition. The successful induction was confirmed by Northern analysis of resulting increased expression of the CWPl gene encoding the cyst wall protein 1. An examination of the encystation process with SEM (scanning electron microscopy) and TEM (transmission electron microscopy) revealed that the trophozoite, a flagellate with a bilateral symmetry, was transformed to a cyst form with an oval-shape and defined filamentous wall. The encystation was found to cause a disappearance of the flagella and an invagination of the adhesive disc. An extensive formation of rER (rough endoplasmic reticulum) was observed after 24h of induction, indication an active synthesis and export of proteins during this process. The vital staining of the invitro-induced systs showed that most cysts maintained their viability.

• Journal of the Korea Furniture Society
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• v.23 no.2
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• pp.185-194
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• 2012

Contemporary society maintains mass-product system that keeps endless cycle of making and consuming. In this vein, everyday life becomes to be under the control of function and efficiency. On the contrary, the people are getting to have a desire of escaping from this everydayness, that is, the desire for non-everydayness. British architect, Nigel Coates understood the potentiality of contemporary metropolis which produce new experiences through their heterogeneities. During 1980s, Japanese economic bubble provided rich nourishment to the desire for non-everydayness based on consumers' tastes. Nigel Coates snatched this phenomena and designed commercial spaces aligned to the non-everydayness. He shows very eloquent version of escaping sense. We can find the exquisite quality of non-everydayness through design vocabulary of object's form and arrangement. In the viewpoint of object form, Coates adopted classical statues of Greek, that is antique, and modern gadgets such as airplane wings and seats. Also, we can find abundant gestures of curvilineal contours throughout the objects he designed. As for the objects' arrangement, he introduced repetition and curved composition that can stimulate human interaction with interior scape.

• Parasites, Hosts and Diseases
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• v.54 no.3
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• pp.357-361
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• 2016

Following the first report of Opisthorchis viverrini infection in a domestic duck in Phu My District of Binh Dinh Province, Central Vietnam, many other cases were observed in the province. We determined the infection rate and intensity of O. viverrini infection in ducks in 4 districts of the province. A total of 178 ducks were randomly selected from 34 farms for examination of flukes in the liver and gall bladder. An infection rate of 34.3% (range 20.7-40.4% among districts) was found; the intensity of infection was 13.8 worms per infected duck (range 1-100). These findings show the role of ducks as a host for O. viverrini, duck genotype, which is sympatric with the human O. viverrini genotype in this province. It also stresses the need for investigations on the zoonotic potential and the life cycle of this parasite.

• Journal of the Korean Society for Precision Engineering
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• v.32 no.4
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• pp.353-358
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• 2015

Energy consumption reduction has become an important key word in manufacturing that can be achieved through the efficient and optimal use of raw materials and natural resources, and minimization of the harmful effects on nature or human society. The successful implementation of this concept can only be possible by considering a product's entire life cycle and even its disposal from the early design stage. To accomplish this idea with milling, minimum quantity lubrication (MQL) strategies and cutting conditions are analyzed through process modeling and experiments. In this study, a model to predict the cutting energy in the milling process is used to find the cutting conditions, which minimize the cutting energy through a Kriging meta-modeling process. The MQL scheme is developed first to reduce the amount of cutting oil and costs used in the cutting process, which is then employed for the entire modeling and experiments.

• Proceedings of the Technology Innovation Conference
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• 2009.02a
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• pp.453-471
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• 2009

현대경제가 점차 지식기반경제로 변화하고 있고, 이러한 지식을 창출하고 활용하여서 경제적 가치로 전환되는 선순환적 체계를 갖추기 위해서는 인재육성이 가장 핵심적인 필수조건일 것이다. 현재 국가의 미래발전의 가장 핵심적인 부분이 과학기술발전전략임을 고려할 때 과학기술인력에 대한 인적자원개발 정책은 필수적인 요소이다. 그렇다면 과학기술발전에 주도적인 역할을 하고 있는 출연(연)에서 효율적인 방향정립이 필요한 이 시점에서 연구원의 인력을 양성하는 일은 국가혁신체제 전반에 걸쳐 경쟁력 강화라는 측면에서 볼 때 매우 중요하다고 볼 수 있다. 본 연구는 기존문헌들을 바탕으로 인력양성에 대한 연구의 틀을 제시하고자 한다. 창조적 인력양성전략은 조직 내에서 유치, 육성, 활용, 퇴직, 안락한 노후로 이어지며, 이를 바탕으로 한 새로운 인력의 유치 등으로 이어지는 것으로 "창조적 인적자원의 전주기적 경영(Life Cycle Management of Creativity Human Capital)" 을 본 연구의 핵심으로 전체연구를 전개하도록 한다. 또한 이와 같은 전주기적 경영에 있어서 각 단계별로 다양한 정책적 이슈들이 대두될 수 있다.

• Bulletin of the Korean Space Science Society
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• 2008.10a
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• pp.32.1-32.1
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• 2008

It is well known that solar and space weather activities can influence the performance and reliability of modern technological system and can endanger human life. Since 2007, the Korea Astronomy and Space Science Institute (KASI) has initiated a research project for the construction of Korean Space Weather Prediction Center (K-SWPC) to make preparations for the next solar cycle maximum (~2012). In this talk, we briefly introduce the current progress of KASI activities for K-SWPC; extension of ground observation system, construction of space weather database and network, development of prediction models, and space weather effects. In addition, future plans for KSWPC will be discussed.

• Korean Journal of Microbiology
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• v.43 no.4
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• pp.237-242
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• 2007

One of the chief characteristics of the retrovirus life cycle is the appearance of provirus caused by integration of viral genome into the host cell genome, and its delivery stably to the next generation as a part of host germ line. This stable form is called endogenous retrovirus (ERV) and expressed by exogenous or endogenous factors. HERVs and MuERVs are present in humans and mice correspondingly, and their expressions frequently cause diseases. Several diseases such as cancer, autoimmunity and neurological disorders are related with HERVs. Therefore, various strategies should be established for the development of effective therapies for the suffering patients.