• 대한본초학회지
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• 제36권3호
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• pp.47-53
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• 2021

Objectives : Currently, the alopecia is one of the most emotionally stressful syndromes in human life. Human hair dermal papilla cells (HDPCs) play an essential role in controlling hair growth and in regulating hair cycle. We performed MTT assay, cell cycle, and western blot to determine the effects of essential oil from Coicis Semen (ECS) on hair growth in HDPCs. Methods : We monitored cell proliferations by MTT assay in HDPCs. After setting up the safe and effective concentration range to be treated ECS, cell cycle analysis was performed using flow cytometry. Also, the protein expression of hair growth-related factors such as insulin like growth factor-1 (IGF-1), Wnt, extracellular signal-regulated kinase (ERK), serine/threonine-specific protein kinase (Akt) in HDPCs was determined by western blot. Results : As results, cell proliferation was increased in ECS group compared to dimethyl sulfoxide (DMSO) group and minoxidil (MNXD) group. Cell number of ECS group was more decrease in sub G1 phase than cell number of DMSO group. Also, cell number of ECS group increased compared to cell number of DMSO group in G1 phase. Protein expression of ECS group was higher than protein expression of DMSO group on related hair growth factors (IGF-1, Wnt, ERK, Akt). Conclusion : As mentioned above, ECS increased cell proliferation and the protein expression of IGF-1, Wnt, ERK, and Akt. These results suggest that ECS could be used as a potential material for the treatment of alopecia by increasing the proliferation of HDPCs.

• Parasites, Hosts and Diseases
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• 제39권2호
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• pp.185-192
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• 2001

To evaluate the factors that determine the transmission level of vivax malaria using vectorial capacity, entomological surveys were conducted from .lune to August, 2000. From 6 nights of human-bait collection in Paju, the human biting rate (ma) was counted as 87.5 bites/man/night. The parity of Anopheles sinensis from human baiting collections fluctuated from 41% to 71% (average 48.8%) of which the rate gradually increased as time passed on: 35.2% in Jun. ; 55.0% in July; 66.2% in Aug. From this proportion of parous, we could estimate the probability of daily survival rate of An. sinensis to be 0.79 assumed with 3 days gonotrophic cycle and the expectancy of infective life through 11 days could be defined as 0.073. Blood meal analysis was performed using ELISA to determine the blood meal source. Only 0.8% of blood meals were from human hosts. We could conclude that An. sinensis is highly zoophilic (cow 61.8%) Malaria is highly unstable (stability index < 0.5) in this area. From these data, vectorial capacity VC) was determined to be 0.081. In spite of a high human biting rate (ma), malaria transmission potential is very low due to a low human blood index. Therefore, we could conclude that malaria transmission by An. sinensis is resulted by high population density, not by high transmission potential. For this reason, we need more effort to decrease vector population and vector-human contact to eradicate malaria in Korea.

• 가정과삶의질연구
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• 제8권2호
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• pp.49-68
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• 1990

Family Life Education(FLE) is an educational specialty which was originated in reaction to changing social conditions, industrialization, and urbanization It deals with the perceived inadequacies of families to cope with these changes, thereby reducing social problems involved with and improving family life. With an assumption that the studies about FLE have not been so active in Korea, the present thesis examines the FLE in Korea; i.e. the definition, the objectives, the scope, the approximation of family life education, and the necessity of marital education in Korea based upon the published theories of FLE in th United Stated of America. Also it attempts to formulate a tentative plan for the promotion of a FLE program in Korea. The concept of FLE in Korea was manifested in the social educational law and life-span education, specified as the 4th among ten presidential regulations; and it has drawn an institutional, governmental attention It is defined as " the life-span developmental education to enhance the quality of human life, to solve family problem, to develop an individual's potentiality, and to strengthen the family correlations." of the FLE programs, the marital education is considered one of the crucial subjects because it is the core of the family life. With this premise, FLE tries to support the healthy marital relations, , subsequently helping to explore the family potentiality and to strengthen family ties, Considering the seemingly dual characteristics of the Korean family types, the contents of marital education program should be consisted of the following; marital communication, role cooperation, and effective expressions of mutual affection, In addition, reciprocal understanding and cooperation of the married couples to overcome the differences of value, personality, hobby, and religion, the educational methods in raising kids, and the effective management of home economics should be included. The objects of the FLE program are unmarried, pre-married, and married persons. Fro the married persons, the FLE program should be arranged in accordance with their marital status divided by the family life cycle so that they may prevent possible family problems at each stage of the family life. Also, to prevent the problems incurred in the curse of carrying out family functions, the FLE program should be provided with on the basis of a family unit, thereby improving the quality of the family functions.

• 혜화의학회지
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• 제19권1호
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• pp.35-48
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• 2010

In Neijing("內經"), it explains heaven, earth, four seasons(天地四時) take part in human's birth, it gives influence on life support, and have organic relationship between body and movement of sun and earth(日月運行) of heaven and earth. Human body and the natural world corresponds, so the time changes in the natural world give immediate influence to human body, and correspond changes happen inside human body. This has no exception in qi of human(人氣), meridians, the viscera and organs(臟腑) and so on. In time, there are many kinds of cycles such as year, month, 10 days and a day. Yin and yang and the five elements in each cycle shows changes of prosperity and decay and transformation. In a year, there are spring, summer, late summer, fall and winter which are each included to wood, fire, earth, metal and water. Spring and summer belong to yang(陽), and fall and winter belong to yin(陰). A day can be divided into the crowing of the cook, dawn, noon, and twilight. After midnight yin falls and yang rises(陰盡陽生), and after noon yang falls and yin rises(陽盡陰生). Ups and downs of the qi and blood and human body change with time and the region of whereabout is different. In one month, when the moon is full qi of blood rises and when the moon comes down qi of blood falls. The qi of meridian(經氣) has a periodical changes with regular movement in meridian. This is a result of continuing movement of meridian and the nutrient(營) and the defense(衛) in human body, stars correspond with ups, and correspond with flow of water of meridian(經水) with downs. In a day the twelve meridians(十二經脈) in hour of yin(hours 3~5), it starts with qi and blood of lung meridian(手太陰肺經) prosperous, each qi and blood of meridian prosperous in order. In eight extra meridians(奇經八脈), Bideungpalbup(飛騰八法) per 5 days, Younggoopalbup(靈龜八法) per 60 days qi of pulse(脈氣) changes correspond. The qi and blood of five meridian points(五兪穴) is 5 days, so.

• 여성건강간호학회지
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• 제6권4호
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• pp.619-631
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• 2000

The purpose of this research is to understand the levels of crisis and quality of life and the relationship between them. Data was collected from questionnaires completed by 384 middle-aged women between 40 and 59 years old. The sense of crisis was measured by a scale which was developed by Kim(1988) and modified by Oh(1999). The quality of life was measured by the scale which was developed by Noh(1988). The data was analyzed using the SPSS (Statistical Package for the Social Sciences) program and included: descriptive statistics, Pearson correlation, and stepwise regression. The results of this study were as follows: 1) In the sense of crisis scale, 'experiencing emptiness and powerlessness through my friends is the highest item($3.56{\pm}1.06$). 'Meaninglessness in daily living' factor's score is $3.16{\pm}0.76$ and another factor, 'concept of my life thus far' score is $2.89{\pm}0.74$. Then the former is higher than the latter. 2) In the quality of life, 'eat well now' is the highest item($3.60{\pm}0.93$). 'self-esteem' is the highest factor($3.37{\pm}0.62$) and 'emotional state' is the lowest factor($2.89{\pm}0.31$). 3) According to the correlation between factors of crisis and quality of life, there was a significant negative correlation between two factors of crisis and five factors of quality of life except emotional state. 4) According to linear regression, two factors of the sense of crisis showed the explanatory power of the quality of life with 31.6%. On the basis of above findings, the following is suggested; Crisis is not precipitated by special events and new accidents, but the normal emotional change phenomena process in the human life cycle. As a result, we should develop the nursing programs and education programs about crisis intervention for middle-aged women's health.

• 생명과학회지
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• 제27권7호
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• pp.834-839
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• 2017

Propolis는 꿀벌들이 나무로부터 수집한 천연물로서 항산화, 항염증, 항암 효과를 가지고 있어 전통의학에서 사용되고 있으며, 이러한 생리활성은 여러 가지 유용성분들이 혼합된 것과 관련이 있다. 난소암은 우리나라 여성에서 두 번째로 발병률이 높은 암이다. 대부분의 난소암 환자들은 초기에 수술적 기법과 항암요법에 우수하게 반응하지만, 항암제 내성에 의한 재발이 발생하게 되면 항암요법제에 의한 반응률이 매우 저조하여 높은 사망률을 보인다. 따라서, 난소암의 높은 치사율을 극복하기 위한 새로운 치료제 및 항암보조제의 개발이 필요하다. 본 연구에서는 인체 상피성 난소암 세포주인 A2780를 이용하여 Austalian propolis의 항암 효과와 활성기전을 조사하였다. Propolis 추출물은 농도 의존적으로 난소암 세포의 증식을 억제했으며. Flow cytometric 분석을 통해 G0/G1기에서 세포 주기 억제와 apoptosis 유도 효과를 확인하였다. 이러한 결과는 Austalian propolis의 인간 난소암에 대한 예방과 치료를 위한 보조제로서의 가능성을 제시한다.

• 생명과학회지
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• 제23권5호
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• pp.622-628
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• 2013

본 연구에서는 마늘에서 유래된 생리활성 물질인 diallyl trisulfide (DATS) 처리에 따른 U937 인체혈구암세포의 증식억제가 apoptosis 및 cell cycle arrest 유발과 관련이 있는지 조사하였다. U937 세포증식은 DATS에 의해 농도 및 시간 의존적으로 감소함을 확인 하였고, 이는 apoptosis에 의한 직접적인 세포죽음과 CDK1 및 cyclin B1의 발현 증가 및 histone H3의 인산화와 연관된 mitotic arrest와 관련이 있음을 알 수 있었다. 또한 DATS 처리 초기에 reactive oxygen species (ROS)의 생성이 매우 증가되었으나, ROS scavenger (N-acetyl-l-cysteine)에 의한 인위적 ROS 생성의 억제는 DATS에 의한 apoptosis 및 mitotic arrest를 완벽하게 차단시켰다. 이는 U937 세포에서 DATS에 의해 유도된 apoptosis 및 mitotic arrest가 ROS에 의해 매개된다는 것을 의미하며, 본 연구의 결과는 DATS가 인체혈구암세포에서 세포증식억제와 관련된 항암기전을 이해할 수 있는 기초자료로서 매우 유용하게 사용될 것이라 생각된다.

• 생명과학회지
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• 제28권12호
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• pp.1469-1476
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• 2018

Warburg 효과의 발생은 고형암에서 화학적 항암제의 내성을 발생시킨다. 따라서 호기성 해당과정과 같은 에너지 대사과정은 암 치료의 중요한 표적으로 알려져 있다. Pyruvate dehydrogenase kinase (PDK) 활성 억제물질로 알려진 dichloroacetate (DCA)는 많은 암세포에서 포도당의 호기성 해당과정을 산화적인산화 과정으로 전환시킬 수 있음이 보고되었다. 이 연구는 치료가 매우 어렵다고 알려진 인간 역분화 갑상선 암세포주인 8505C의 성장에 미치는 DCA효과를 조사하였다. DCA는 정상 갑상선 세포주의 성장에는 영향을 주지 않은 반면 8505C 세포주의 성장을 특이적으로 저해하였다. DCA의 처리에 의해 8505C 세포주는 $HIF1{\alpha}$, PDK1, Bcl-2와 같은 항-세포자살 관련 단백질들의 발현이 감소하고, Bax와 p21과 같은 세포자살 유도 단백질과 세포주기 억제 단백질의 증가로 인하여 세포주기 정지와 세포자살 유도에 의해 성장이 억제되었다. 이런 세포의 변화는 DCA 처리에 의한 활성산소족의 생산이 증가하고, 포도당 대사가 호기성 해당과정에서 산화적인산화 과정으로 전환되었기 때문이란 것을 확인하였다. 흥미롭게도, DCA는 포도당 대사과정의 변화뿐만 아니라 sodium/iodine symporter (NIS)의 발현양도 증가시킨다는 것을 확인하였다. 이 연구의 결과로 PDK 활성 저해제는 치료하기 힘든 역분화 갑상선 암 치료제로 이용할 수 있고, 또한 역분화 갑상선 암에 대한 방사능 치료의 효능을 높일 수 있을 것으로 기대된다.

• KIEAE Journal
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• 제1권1호
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• pp.11-16
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• 2001

Concern about global environment has been increasing in recent years. Efforts to minimize the environmental impact to the globe as well as human beings have increased, especially in the late twentieth century. The study researches one of the solutions for the environmentally friendly building construction, which can contribute to sustaining the world environment. Assessment and proposals are made for high-rise apartments, one of the most popular construction types nowadays in Korea. Careful building design and construction for apartment buildings could reduce the environmental impact significantly without lowering standards of living. Since the volume of high-rise apartment construction is so great, a small improvement in each building will make a great contribution. Assessments are made over the life-span of apartment buildings. A tool has been made through references from western examples. mainly from BHEEAMs made in the United Kingdom. The components of the tool include passive design strategies, building materials. energy consumption during building operation, and management and maintenance. Co-relationship between buildings and ten environment in Korea is searched. Korean high-rise apartments are assessed with the tool and suggestions for sustainable development are made mainly for improvement of embodied energy of building materials and the life of buildings.

• BMB Reports
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• 제42권1호
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• pp.6-15
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• 2009

The most common genetic disorder Down syndrome (DS) displays various developmental defects including mental retardation, learning and memory deficit, the early onset of Alzheimer's disease (AD), congenital heart disease, and craniofacial abnormalities. Those characteristics result from the extra-genes located in the specific region called 'Down syndrome critical region (DSCR)' in human chromosome 21. In this review, we summarized the recent findings of the DYRK1A and RCAN1 genes, which are located on DSCR and thought to be closely associated with the typical features of DS patients, and their implication to the pathogenesis of neural defects in DS. DYRK1A phosphorylates several transcriptional factors, such as CREB and NFAT, endocytic complex proteins, and AD-linked gene products. Meanwhile, RCAN1 is an endogenous inhibitor of calcineurin A, and its unbalanced activity is thought to cause major neuronal and/or non-neuronal malfunction in DS and AD. Interestingly, they both contribute to the learning and memory deficit, altered synaptic plasticity, impaired cell cycle regulation, and AD-like neuropathology in DS. By understanding their biochemical, functional and physiological roles, we hope to get important molecular basis of DS pathology, which would consequently lead to the basis to develop the possible therapeutic tools for the neural defects in DS.