• Reproductive and Developmental Biology
• /
• 제31권4호
• /
• pp.221-226
• /
• 2007

Embryonic stem (ES) cells are known to have an infinite proliferation and pluripotency that are associated with complex processes. The objective of this study was to examine expression of genes differentially regulated during differentiation of human ES cells by suppression subtractive hybridization (SSH). Human ES cells were induced to differentiate into neural precursor cells via embryoid body. Neural precursor cells were isolated physically based on morphological criteria. Immunocytochemical analysis showed expression of pax6 in neural precursor cells, confirming that the isolated cells were neural precursor cells. Undifferentiated human ES cells and neural precursor cells were subject to the SSH. TPX2 (Targeting Protein for Xklp2 (Xenopus centrosomal kinesin-like protein 2)) was identified, cloned and analyzed during differentiation of human ES cells into neural lineages. Expression of TPX2 was gradually down-regulated in embryoid bodies and neural precursor cells relative to undifferentiated ES cells. Targeting Protein for Xklp2 has been shown to be involved in cell division by interaction with microtubule development in cancer cells. Taken together, result of this study suggests that TPX2 may be involved in proliferation and differentiation of human ES cells.

• Asian Journal for Public Opinion Research
• /
• 제2권1호
• /
• pp.3-7
• /
• 2014

How satisfied or dissatisfied people are with aspects of daily life such as housing, income, health, family, food, human relations, and work provides vital information about them. On the basis of a nation-wide random sample survey in Japan in October 2013, this article analyzes Japanese citizens' daily life satisfaction in a snapshot. The big picture of Japanese daily life is that they are more or less satisfied with their daily life, although income, work, and housing register lower levels of satisfaction in comparison to other aspects like food, family, human relations, and health. Their satisfaction is focused on food, family, and human relations.

• 한국가정과학회지
• /
• 제1권1호
• /
• pp.66-80
• /
• 1998

The purposes of this study were to analyze the family life, farm wort and leisure satisfaction of rural wives in Chonnam district. 300 farmhouses were selected by stratified sampling from 20 Myon in Chonnam district, 283 wives were interviewed using questionnaire. The results are as follow : 1) The level of wives's value perception of agriculture was low. The level of satisfaction of family life was higher than farm and leisure life. The farm satisfaction is the lowest. 2) family environment variables(such as age, education, children number, yearly income, family type, the number of year of house built), variables connected with farm life(major type of agriculture, value perception of agriculture, underdeveloped cause, living period in farm), and variables connected with time use significantly influenced on the life satisfaction. To promote the satisfaction of rural wives various implications were discussed. (Korean J Human Ecology 1(1) : 66∼80, 1998)

• 한국가정과학회지
• /
• 제1권2호
• /
• pp.75-88
• /
• 1998

This study designed to explore the patterns of housing life-styles and the characteristics of apartment housing residents and to examine the relationships between housing life-styles and the characteristics of apartment-housing residents. The data for this study was collected by questionnaires administered to 278 homemakers living in apartment housing over 20s pyong(66$m^2$) in the Kwangju area. The major findings of this study can be summarized as follows; 1. Housing life-styles were grouped into 9 sub-categories : the Anbang-centered, the dining-centered, the pursuit of conspicuosness and conformity, orientation to change, the pursuit of orderliness, the pursuit of decoration, orientation to community, pursuit of individuality and the orientation to a natural environment. 2. Housing life-styles were significantly different according to age, educational background, and employment status of homemakers, occupation of the primary income provider, family-life cycle stage, total household income, and housing size. (Korean J Human Ecology 1(2):75~88. 1998)

• Structural Engineering and Mechanics
• /
• 제85권4호
• /
• pp.461-468
• /
• 2023

Edible coatings are one of the most innovative methods to preserve the quality and increase the shelf life of fresh fruits and vegetables. A successful edible coating should have a barrier against gases, especially oxygen and water vapor, and have good surface characteristics. Today, chitosan coating is widely used due to its properties, such as non-toxic, biodegradable, and biocompatibility. Is. Coating the surface of fruits and vegetables with chitosan increases shelf life due to reducing weight loss and reducing respiration rate and also reduces decay due to its antimicrobial and anti-fungal effect. This work discusses the effect of using chitosan coating containing chamomile extract to increase fresh vegetables'shelf life. In addition to increasing the shelf life of vegetables, this method can be used as a solution for the economic management of human resources. The results of this method confirm the successful synthesis of these nanoparticles, and the results of applying this food coating on vegetables have been successful. They have increased the shelf life of vegetables such as basil and spinach.

• Asian Pacific Journal of Cancer Prevention
• /
• 제13권6호
• /
• pp.2961-2966
• /
• 2012

Extremely low frequency electro magnetic fields (EMFs) have been classified as possibly carcinogenic to humans by the International Agency for Research on Cancer. An increased number of chromosomal alterations in peripheral lymphocytes are correlated with elevated incidence of cancer. The aim of the present study was to assess occupationally induced chromosomal damage in EMF workers exposed to low levels of radiation. We used conventional metaphase chromosome aberration (CA) analysis and the micronucleus (MN) assay as biological indicators of non ionizing radiation exposure. In the present study totally 70 subjects were selected including 50 exposed and 20 controls. Informed written consent was obtained from all participants and the study was performed in accordance with the Declaration of Helsinki and the approval of the local ethical committee. A higher degree of CA and MN was observed in exposed subjects compared to controls, the frequency of CA being significantly enhanced with long years of exposure (P<0.05). Moreover increase in CA and MN with age was noted in both exposed subjects and controls, but was significantly greater in the former. The results of this study demonstrated that a significant induction of cytogenetic damage in peripheral lymphocytes of workers occupationally exposed to EMFs in electric transformer and distribution stations. In conclusion, our findings suggest that EMFs possess genotoxic capability, as measured by CA and MN assays; CA analysis appeared more sensitive than other cytogenetic end-points. It can be concluded that chronic occupational exposure to EMFs may lead to an increased risk of genetic damage among electrical workers.

• Child Health Nursing Research
• /
• 제16권4호
• /
• pp.287-296
• /
• 2010

Purpose: The purpose of this study was to explore and understand life and meaning in life of elementary school students. Methods: Data from 1,790 higher grade elementary school students were analyzed for content of meaning in life. Results: The students described the following: 1) Meanings of living existence included, 'living things', 'importance', 'human life', 'family', 'event', 'eternity', 'origin of life', 'disease', and, 'protection'. 2) Four categories were found for reasons why human life is important, 'solitary', 'limitation', 'valuables', and 'living'. 3) Things that one can do to protect the value of human life were, 'doing safely', 'keep things well', 'altruistic behavior', 'doing sound activity', 'preservation & development', and 'devotion of oneself'. 4) Meaning of life included 'to accomplish plans', and 'to do things' 5) Cases when students felt life had meaning were 'perform meaningful behavior', 'comfortable emotionally', and 'receiving help from others'. 6) The methods by which to live a meaningful life were 'working diligently', 'serving', and 'doing rightly'. Conclusion: These results suggest that students recognize the meaning of life in a most positive way and with a relatively wide scope, but as a few students had a negative orientation, age-appropriate and more systematic education programs are needed.

• Preventive Nutrition and Food Science
• /
• 제16권4호
• /
• pp.394-407
• /
• 2011

Better understanding of the complex factors leading to human diseases will be necessary for both long term prevention and for managing short and long-term health problems. The underlying causes, leading to a global health crisis in both acute and chronic diseases, include finite global health care resources for sustained healthy human survival, the population explosion, increased environmental pollution, decreased clean air, water, food distribution, diminishing opportunities for human self-esteem, increased median life span, and the interconnection of infectious and chronic diseases. The transition of our pre-human nutritional requirements for survival to our current culturally-shaped diet has created a biologically-mismatched human dietary experience. While individual genetic, gender, and developmental stage factors contribute to human diseases, various environmental and culturally-determined factors are now contributing to both acute and chronic diseases. The transition from the hunter-gatherer to an agricultural-dependent human being has brought about a global crisis in human health. Initially, early humans ate seasonally-dependent and calorically-restricted foods, during the day, in a "feast or famine" manner. Today, modern humans eat diets of caloric abundance, at all times of the day, with foods of all seasons and from all parts of the world, that have been processed and which have been contaminated by all kinds of factors. No longer can one view, as distinct, infectious agent-related human acute diseases from chronic diseases. Moreover, while dietary and environmental chemicals could, in principle, cause disease pathogenesis by mutagenic and cytotoxic mechanisms, the primary cause is via "epigenetic", or altered gene expression, modifications in the three types of cells (e.g., adult stem; progenitor and terminally-differentiated cells of each organ) during all stages of human development. Even more significantly, alteration in the quantity of adult stem cells during early development by epigenetic chemicals could either increase or decrease the risk to various stem cell-based diseases, such as cancer, later in life. A new concept, the Barker hypothesis, has emerged that indicates pre-natal maternal dietary exposures can now affect diseases later in life. Examples from the studies of the atomic bomb survivors should illustrate this insight.

• 한국환경교육학회지:환경교육
• /
• 제22권3호
• /
• pp.49-62
• /
• 2009

This study started from the need to change the technocentric view of environment into the ecocentric one to overcome ecological crisis today. Thus, the purposes of this study are to reorganize characteristics of ecocentrism, to analyze ecocentrism of Native North Americans as a realistic model, and to finally find suggestions to environmental education. The results of this study can be summarized as follows; Frist, the ecocentric perception on relationship between human beings and nature pursues co-existence through harmony and balance based on holistic understandings. And the ecocentric perception on relationship among human beings attempts to preserve environment by overcoming rule structure among them. The ecocentric perception on relationship between human beings and spirituality is to set life goals to achieve spirituality rather than to pursue material life. Second, Native North Americans have a holistic world view that human beings and nature are an inseparable one through the symbol of 'the Sacred Circle'. They also lived simple lives which satisfies vital needs instead of pursuing material lives in terms of the perception on relationship among human beings. When it comes to the perception on relationship between human beings and spirituality, they feel sacredness from all things in daily life and want to have peaceful relationships with their own inside through spirituality toward 'Mother Earth'. Third, the ecocentrism of Native North Americans make suggestions that environmental education confirm again what roles of human beings are in ecosystem, and that it helps students develop ecological sensitivity by giving them many opportunities to go to field trips. Furthermore, environmental education helps them positively think about and actively choose simple life. In addition, it has to be designed to help students seek sacredness in daily life through ecological imagination. This study has values of newly organizing characteristics of each ecocentric idea by integrating it from complementary aspects, and pursuing direction of ecocentrism and its realistic possibility by analyzing the ecocentrism of Native North Americans as a model of ecological life.

• Human Ecology Research
• /
• 제60권2호
• /
• pp.243-256
• /
• 2022

Undergraduate freshmen are currently encountering numerous difficulties in adapting to college life due to the outbreak of the COVID-19 pandemic. Thus, several studies have underlined the need to develop interventions focused on undergraduate students' resilience during this situation. We adopted the service design method in identifying the difficulties faced by undergraduate freshmen during this pandemic, and also developed interventions for raising their resilience. The service design method is a person-centered approach which adopts the user's viewpoint in developing solutions, thereby enabling their requirements to be satisfied. Surveys and in-depth interviews revealed that undergraduate freshmen were experiencing psycho-emotional problems which were derived from schoolwork and college life. This is particularly the case with students who are experiencing greater levels of academic difficulty, and were insufficiently equipped with resources such as stress-coping strategies and social support. Four undergraduate freshmen were recruited online to test the effectiveness of a resource promotion program aimed at enhancing their resilience. This program has proved to be helpful in relieving daily/academic stress and in building relationships among freshmen, regarding which the participants showed a high level of satisfaction. The results were discussed by focusing on psycho-emotional difficulties and resources of college freshmen, as well as the effectiveness of the resource promotion program. Further research is required in order to verify the efficiency of the program and to confirm conclusions.