• 한국동물학회:학술대회논문집
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• 한국동물학회 2003년도 한국생물과학협회 학술발표대회
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• pp.212.3-212
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• 2003

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• 한국안전학회지
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• 제18권3호
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• pp.126-130
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• 2003

The term stress is currently used to cover a wide variety of phenomena, ranging from physical to social and cultural factors. The term has defined psychological stress as an imbalance between perceived or subjective demand and perceived response capability. The behavior science model has provided the accident proneness through the life change unit factors considering human behavior, life style, ideas, culture, and psychological state. Therefore, this paper presents the Korean life change unit model through statistical testing with the proposed life change unit factors on the unmarried workers living in the middle area. The proposed model can be simply used in real fields in order to minimize the industrial accidents.

• Reproductive and Developmental Biology
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• 제31권3호
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• pp.187-191
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• 2007

본 연구는 사람 H-transferase가 과발현하는 돼지 체세포주를 개발하는데 있다. 돼지 세포에 사람 H-transferase 유전자를 발현시키는 것은 이종간 장기 이식에 있어서 초급성 거부 반응을 방지하기 위한 한 가지 방법이다. 본 연구에서는 과발현 벡터를 구축하기 위하여 사람 H-transferase을 HepG2 세포로부터 동정하였으며, 이 유전자를 CMV promoter를 이용하여 발현할 수 있도록 포유동물 발현 벡터인 pRc/CMV벡터에 삽입하였다. 또한, 돼지 산자의 귀 세포를 이용하여 체세포를 수립한 후 jetPEI DNA transfection reagent를 이용하여 벡터를 도입하였고, $300{\mu}g/ml$의 G418로 12일간 선별하였다. PCR을 이용하여 선별된 colony들을 분석한 결과, 벡터가 도입되었음을 확인하였고, RT-PCR을 이용하여 사람 H-transferase mRNA가 발현하는 것을 확인하였다. 본 연구에서 확립된 세포주는 사람 H-transferase가 과발현하는 형질 전환돼지의 생산에 이용될 수 있을 것으로 생각된다.

• 한국가정과학회지
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• 제7권4호
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• pp.83-98
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• 2004

The purpose of this study is to provide basic information necessary for improving the satisfaction and quality of leisure life and thereon propose better leisure life programs in accordance with gender. For the purpose, this researcher made a questionnaire survey of 560 persons between July and December 2003 to know the trend and satisfaction of their leisure life in accordance with their gender. Results of the study can be summarized as below; First. the extent to which the surveyed persons participated in leisure life was highest in terms of other leisure activities, followed by self-development. viewing activities, sports activities and journey in order. The satisfaction of leisure life participation by those surveyed persons was scored 3 or over in average, meaning 'generally satisfied'. The time of leisure life participation was most spent in other leisure activities and self-development. Expenses of the participation were highest in terms of other leisure activities. followed by self-development and viewing activities in order. Second, differences in sub-areas of leisure life were analyzed in accordance with gender to show that the degree and time of participation in viewing activities and self-development, expenses of sports activity participation and the degree of participation in journey and others were significantly different between the opposite sexes which were surveyed here. Men were higher in the degree, time and expenses of the participation than women. Third. effects of gender on the satisfaction of leisure life as a whole were determined here to show that men were higher in such satisfaction when they were more satisfactorily participating in sport activities while in case of women, when they were spending more time in sport activities. Women showed a higher satisfaction of leisure life as a whole when they were more frequently and satisfactorily participating in other leisure activities. Based on these results, the researcher proposed effective leisure life programs in accordance with gender.

• Asian Pacific Journal of Cancer Prevention
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• 제14권4호
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• pp.2295-2299
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• 2013

Background: To explore the role of caveolin-1(CAV-1) gene silencing on MAPK activation in lipopolysaccharide (LPS)-challenged human mammary epithelial cells. Methods: We established a MCF-10ACE of CAV-1 gene silencing from human mammary epithelial cell line MCF-10A by RNAi technology. DNA Microarray were used to detect the expression of inflammation-associated genes in MCF10ACE. Western blotting was used to examine the activation of MAPK in lipopolysaccharide(LPS)-challenged MCF-10A and MCF-10ACE. Moreover, immunofluorescence and Western bloting were performed to detect the co-localization of CAV-1 and toll-like receptor 4 (TLR4) in human mammary epithelial cells. Results: MCF-10ACE exhibited significant increases in inflammation-associated gene expression, especially IL-6 (~7-fold) and IL6R (~17-fold). In addition, LPS-induced p38 MAPK and JNK MAPK activation was significantly increased in MCF-10ACE. Furthermore, CAV-1 co-localized with TLR4 and appeared a negative correlation trend. Conclusion: CAV-1 gene silencing promotes MAPK activation via TLR4 signaling in human mammary epithelial cells response to LPS.

• 수산해양기술연구
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• 제47권3호
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• pp.257-266
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• 2011

Recently, the manufacturers of life-jacket are very interested in the acquisition of USCG(US Coast Guard) approval because the acquisition of USCG approval has an important role in the purchasing decision of the buyer's. Be based on criterion of USCG, we studied how to predict the change of floating position of human model with life-jacket to verify the backside restore. For this, in this study, the human model and the lifejacket was modeled in three dimension, the application program for prediction of floating position was developed, and plugged-in commercial program.

• 한국조사연구학회:학술대회논문집
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• 한국조사연구학회 2005년도 추계학술대회 발표논문집
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• pp.247-262
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• 2005

The purpose of this study was to compare the school life of Korean high school students between public and proprietary schools, especially focused on their internal experiences and flow. Internal experiences were composed of three dimensions: emotion, cognitive efficiency, and satisfaction. Flow was classified into four conditions: apathy, boredom, anxiety, and flow. The study results are: 1. Korean high school students felt more flow when they were in public school than in proprietary school,. In public school, they felt relatively more bored, and on the other hand, they were relatively more anxious in proprietary school. 2. Korean high school students felt more positive emotions when they were in public school than in proprietary school. 3. Korean high school students were more involved into study when they were in public school than in proprietary school. 4. Korean high school students were more satisfied when they were in public school than in proprietary school.

• 간호행정학회지
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• 제4권1호
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• pp.19-31
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• 1998

The purpose of this study was to identify whether ethical values of nursing students are deontological or utilitarinian. The subjects of this study were consisted of 163 nursing students. The data was collected from September 3 to 30, 1997. Sub-scale of Nursing Ethical Values(Lee, 1990) were used to measure human life area, nurse-patient relationship area, nurse-nursing task relationship area, nurse-colleague relationship area. Data were analyzed by SPSS for Windows program. The results of this study were summarized as follows: 1. The students took the position of utilitarian slightly in human life area. 2. The students took deontological position slightly in nurse-patient relationships area. 3. The students took deontological position slightly in nurse-nursing task relationships area. 4. The students took deontological position greatly in nurse-colleague relationships area. 5. There were significant relationship between nurse-colleague relationship area and both human life area and nurse-patient relationship area(p< .01). 6. The ethical values of human life area related to religion(p< .01) and attitude of nursing(p< .05). The ethical values of nurse-colleague relationships area related to attitude of nursing(p< .01).

• 생약학회지
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• 제26권4호
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• pp.360-367
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• 1995

The metabolism of ginseng saponins by human intestinal bacteria was studied using human feces under anaerobic culture conditions. $Ginsenoside-Rb_1$, $-Rb_2$ and -Rc(protopanaxadiol type) were mainly metabolized to compound-K(C-K), $20-O-[{\alpha}-L-arabinopyranosyl(1{\rightarrow}6)-{\beta}-{_D}-glucopyranosyl]-20(S)-protopanaxadiol(compound-Y,\;C-Y)$, $20-O-[{\alpha}-L-arabinopyranosyl(1{\rightarrow}6)-{\beta}-{_D}-glucopyranosyll-20(S)-protopanaxadiol(ginsenosied-MC,{\;}MC)$, respectively, and $ginsenoside-Rg_1$ and -Re(protopanaxatriol type) to their aglycon, 20(S)-protopanaxatriol, though the pathway and rate of the metabolism were affected by fermentation medium. C-K was not decomposed any more, while C-Y and Mc were both gradually hydrolyzed to C-K.

• 동의신경정신과학회지
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• 제11권2호
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• pp.177-187
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• 2000

The purpose of this thesis is to make manifest the relation of Gigong- Yangsaeng(good life) and The old-health care, and through the conclusion, to study that how much the method of Gigong-Yangsaeng con entertain with refreshments to the stop of being old. Especially this thesis managed to divide the effect of the stop of being old by Gigong-Yangsaeng 3 parts and studied, the first effect is mental that, the second effect is the increase of immunity and the third effect is that of the stop of being old by chemical reaction.The results were as follows:The purpose of the method of Gigong-Yangsaeng is to increase the strength of human body and to resist disease and live long by training and practicing ourselves'Gi(energy of power), People call the method of good life and being old like this Gigong-Yangsaeng. Gigong-Yangsaeng is a method of Yangsaeng(good life) to pull off the purpose of being healthy and strong. We can get some effects of Gigong-Yangsaeng by uniting human mind and body into one. Entirely the key of those effects depends on Jo-sin(to control human body). Jo-sik(to control breath). Jo-sim(to control human mind).