• The Journal of Korean Society of Virology
• /
• v.27 no.1
• /
• pp.1-8
• /
• 1997

Human caliciviruses (HuCVs) cause sporadic cases and outbreaks of acute gastroenteritis (AGE). Three major genogroups of HuCVs have been described including the Norwalk virus (NV)-, the Snow Mountain virus (SMA)-, and the Sapporo-genogroups. This study describes the detection and genetic variation of HuCVs from hospitalized infants with AGE in Korea by RT-PCR and sequencing. The cDNA fragments of 206 to 470bp corresponding to the region of 3 primer pairs (36/35, 35/51 or 3/51) in the polymerase region of NV were generated. Of 185 stools screened, 8% were positive by RT-PCR and their sequences showed that all strains contained the GLPSG and YGDD motifs which are conserved for HuCVs. Amino acid (aa) sequence analysis showed that these strains can be divided into 3 major genogroups. High conservation was observed in that one strain shares 100% of aa sequence with Southampton virus, another shares 99% with the Sapporo virus, and six strains share 90 to 95% with Snow Mountain virus. However, significant sequence variation was also found in other strains. This study indicates that all major genogroups of HuCVs are circulating in Korea.

• The Journal of the Korean Society for Microbiology
• /
• v.19 no.1
• /
• pp.79-83
• /
• 1984

To determine the existence of anti-HLA antibodies finally in 220 human placental extracts to be proved negative antiserum by previous anti-HLA A,B,C antibody screening procedure, the present study was performed by fractionation of immunoglobulins using saturated ammonium sulfate and by simple batch method on DEAE cellulose. Thereafter using known 150 T-lymphocyte panels, complement-dependent microlymphocytotoxicity test was performed to observe the existence of anti-HLA antibodies and the degree of the antibody response of the concentrates. The following results were obtained: 1. Of total 141 placental sera concentrated 45 cases(31.9%) were showed significant anti-HLA A,B,C antibody response after concentration(Excellent, 19(13.5%), Good, 3(2.1%), Weak, 23(16.3%)). 2. Anti-HLA specificities of placental sera obtained after concentration were A2, A24, B13, B27, B44, B51, CN1, C7. 3. A new type C new-1 anti-HLA antibody that is only expressed in Korean people, was obtained. 4. 79 placental sera purrified by simple batch method using DEAE cellulose were showed negative anti-HLA antibody responses.

• The Journal of Advanced Prosthodontics
• /
• v.6 no.6
• /
• pp.555-558
• /
• 2014

Focal epithelial hyperplasia (FEH) is a human papillomavirus (HPV)-induced alteration of the oral mucosa that presents with a clinically distinct appearance. While other HPV-infected lesions such as squamous papilloma, verruca vulgaris, and condyloma acuminatum involve the skin, oral mucosa, and genital mucosa, FEH occurs only in the oral mucosa. The affected oral mucosa exhibits multiple papules and nodules with each papule/nodule being flat-topped or sessile. The affected region resembles the normal color of oral mucosa rather than appearing as a white color since the epithelial surface is not hyperkeratinized. Almost all cases present with multiple sites of occurrence. This rare, benign epithelial proliferation is related to low-risk HPV, especially HPV-13 and -32, and is not transformed into carcinoma. We report a case of FEH that arose on the attached gingiva of an East Asian male adult related to prosthesis without detection of any HPV subtype in HPV DNA chip and sequencing.

• Journal of dental hygiene science
• /
• v.13 no.3
• /
• pp.354-357
• /
• 2013

Verruca vulgaris is one of the most frequent lesions of the skin. Evidences support that this lesion has strong association with low-risk human papillomavirus. Unlike skin lesion, oral verrucae vulgaris are not easily encountered and often misdiagnosed as squamous papilloma. Herein, we reported eight cases of oral verruca vulgaris and analyzed their clinicopathological features.

• Journal of Microbiology
• /
• v.44 no.2
• /
• pp.162-170
• /
• 2006

Oysters are known to be carriers of food-born diseases, but research on viruses in Korean oysters is scarce despite its importance for public health. We therefore tested oysters cultivated in Goheung, Seosan, Chungmu, and Tongyeong, for viral contamination using cell culture and integrated cell culture PCR (ICC-PCR) with Buffalo green monkey kidney (BGMK) and human lung epithelial (A549) cells. Additional screens via PCR, amplifying viral nucleic acids extracted from oysters supplemented our analysis. Our methods found 23.6 %, 50.9 %, and 89.1 % of all oysters to be positive for adenoviruses when cell culture, ICC-PCR, and direct PCR, respectively, was used to conduct the screen. The same methodology identified enteroviruses in 5.45%, 30.9%, and 10.9% of all cases. Most of the detected enteroviruses (81.3%) were similar to poliovirus type 1; the remainder resembled coxsackievirus type A1. A homology search with the adenoviral sequences revealed similarities to adenovirus subgenera C (type 2, 5, and 6), D (type 44), and F (enteric type 40 and 41). Adenovirus-positive samples were more abundant in A549 cells (47.3%) than in BGMK cells (18.2 %), while the reverse was true for enteroviruses (21.8 % vs. 14.5 %). Our data demonstrate that Korean oysters are heavily contaminated with enteric viruses, which is readily detectable via ICC-PCR using a combination of A549 and BGMK cells.

• Proceedings of the Korean Society for Bioinformatics Conference
• /
• 2006.02a
• /
• pp.98-107
• /
• 2006

Chromosomal copy number changes (aneuploidies) are common in human populations. The extra chromosome can affect gene expression by whole-genome level. By gene expression microarray analysis, we want to find aberrant gene expression due to aneuploidies in Klinefelter (+X) and Down syndrome (+21). We have analyzed the inactivation status of X-linked genes in Klinefelter Syndrome (KS) by using X-linked cDNA microarray and cSNP analysis. We analyzed the expression of 190 X-linked genes by cDNA microarray from the lymphocytes of five KS patients and five females (XX) with normal males (XY) controls. cDNA microarray experiments and cSNP analysis showed the differentially expressed genes were similar between KS and XX cases. To analyze the differential gene expressions in Down Syndrome (DS), Amniotic Fluid (AF)cells were collected from 12 pregnancies at $16{\sim}18$ weeks of gestation in DS (n=6) and normal (n=6) subjects. We also analysis AF cells for a DNA microarray system and compared the chip data with two dimensional protein gel analysis of amniotic fluid. Our data may provide the basis for a more systematic identification of biological markers of fetal DS, thus leading to an improved understanding of pathogenesis for fetal DS.

• Journal of the Korea Safety Management & Science
• /
• v.11 no.1
• /
• pp.1-6
• /
• 2009

The specific feature of trains as a means of transportation is that, on one side, at once they can carry big loads but, at the same time, if an accident occurs, it potentially leads to many human casualties or big material losses. Especially, train accidents caused by bad weather conditions result in many fatal losses of human lives and property. In Korea many railways run either in mountainous areas or along rivers thus making them especially susceptible to natural hazards. The types of damages inflicted by heavy rains resulting from rapidly changing meteorological conditions are diverse; and not only their scope is big but also they repeat regularly. Consequently, this study analyses the reasons why such effects of heavy rains on the railway conditions, damage to the railways caused by heavy rains or cases of stone fall as well as other types of accidents are not avoided. Study also, on the basis of laws related to movement in poor weather conditions and specifics of train braking, identifies systematic and technical problems and suggests and emphasizes new complex measures on their prevention.

• Proceedings of the KIEE Conference
• /
• 1998.11b
• /
• pp.615-617
• /
• 1998

This paper proposes a new segmentation method of human races from grey scale images with clutter using a racial template and elliptical structure of the human head. Face detection technique can be applied in many areas of image processing such as face recognition, composition and computer graphics. Until now, many researches about face detection have been conducted, and applications in more complicated conditions are increasing. The general case is more in a complicated background than in a simple one, and a image with not only one face. Research and development of face detection in such a general case are growing rapidly, and the necessity for that is increasing continuously. Sirohey proposed a face detection method using linearized elliptical equation. The method designed in this paper is improved to be applicable even in the more general cases like where the face is much smaller than the image size and with many faces in one image using template matching and elliptic fitting technique.

• Journal of Institute of Control, Robotics and Systems
• /
• v.16 no.5
• /
• pp.415-419
• /
• 2010

A variety of methods for sound source localization have been developed and applied to several applications such as noise detection system, surveillance system, teleconference system, robot auditory system and so on. In the previous work, we proposed the sound source localization using the spatially mapped GCC functions based on TDOA for robot auditory system. Performance of the proposed one for the noise effect and estimation resolution was verified with the real environmental experiment under the single source assumption. However, since multi-talker case is general in human-robot interaction, multiple source localization approaches are necessary. In this paper, the proposed localization method under the single source assumption is modified to be suitable for multiple source localization. When there are two sources which are correlated, the spatially mapped GCC function for localization has three peaks at the real source locations and imaginary source location. However if two sources are uncorrelated, that has only two peaks at the real source positions. Using these characteristics, we modify the proposed localization method for the multiple source cases. Experiments with human speeches in the real environment are carried out to evaluate the performance of the proposed method for multiple source localization. In the experiments, mean value of estimation error is about $1.4^{\circ}$ and percentage of multiple source localization is about 62% on average.

• Korean Institute of Interior Design Journal
• /
• v.21 no.3
• /
• pp.76-86
• /
• 2012

21st century has different characters from 20th century which was an era of machine and rationality based on the industrial revolution. With the advent of the digital revolution, it became an era of pluralism, culture, and emotion. The digital technology made it possible to connect the whole world together in real time and brought about the entirely new notion of time and space. It also dramatically altered the world view. Now we have a different set of social and cultural values from the past. This paper researched the influences of these social and cultural changes on public designs. The leading trends of public designs were analyzed and the successful cases were studied. The main research methodologies were the document review and the instrumental case study. The major social & cultural paradigms of a present era were classified as 'pluralism', 'digital & information revolution', 'human-centered value (against machine-centered value of 20th century)', and 'organic world view'. Each of the classified paradigms was analyzed more to find out the influences on the various trends of public designs. 'Pluralism' has influence on 'experiential public design', 'community art', and 'public design of local values'. 'Digital & information revolution' has influence on 'content-centered public design', 'smart public design', 'immaterial public design', and 'performance in public design'. 'Human-centered value' has influence on 'universal design approach in public design', and 'emotional public design'. 'Organic world view' has influence on 'sustainable public design', and 'ecological public design'.