• Asian Pacific Journal of Cancer Prevention
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• 제15권4호
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• pp.1531-1537
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• 2014

Runt-related transcription factor 3 (RUNX3) is a tumor suppressor gene whose reduced expression may play an important role in the development and progression of esophageal squamous cell cancer (ESCC). The aim of this study was to investigate the clinical relevance of RUNX3 in ESCC patients and effects of overexpression on biological behaviour of Eca109 cells in vitro and in vivo. Immunohistochemistry was performed to detect the clinical relevance of RUNX3 and lymph node metastasis in 80 ESCC tissues and 40 non-cancerous tissues using the SP method. RT-PCR and Western blotting were applied to assess the RUNX3 level and verify the Eca109 cell line with stable overexpression. Localization of RUNX3 proteins was performed by cell immunofluorescence. CCK-8 and Scrape motility assays were used to determine proliferation and migration and the TUNEL assay to analyze cell apoptosis. Invasive potential was assessed in cell transwell invasion experiments. In nude mice, tumorigenesis in vivo was determined. Results showed decreased expression of RUNX3 in esophageal tissue to be significantly related to lymph node metastasis (LNM) (P<0.01). In addition, construction of a recombinant lentiviral vector and transfection into the human ESCC cell line Eca109 demonstrated that overexpression could inhibit cell proliferation, migration and invasion, and induce apoptosis. The in vivo experiments in mice showed tumorigenicity and invasiveness to be significantly reduced. Taken together, our studies indicate that underexpression of RUNX3 in human ESCC tissue is significantly correlated with progression. Restoration of RUNX3 expression significantly inhibits ESCC cells proliferation, migration, invasion and tumorigenesis.

• Asian Pacific Journal of Cancer Prevention
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• 제17권8호
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• pp.4019-4023
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• 2016

Background: Colorectal cancer (CRC) or bowel cancer is one of the most important cancer diseases, needing serious attention. The cell surface receptor gene human epidermal growth factor receptor (EGFR) may have an important role in provoking CRC. In this pharmaceutical era, it is always attempted to identify plant-based drugs for cancer, which will have less side effects for human body, unlike the chemically synthesized marketed drugs having serious side effects. So, in this study the authors tried to assess the activity of two important plant compounds, ferulic acid (FA) and p-coumaric acid (pCA), on CRC. Materials and Methods: FA and pCA were tested for their cytotoxic effects on the human CRC cell line HCT 15 and also checked for the level of gene expression of EGFR by real time PCR analysis. Positive results were confirmed by in silico molecular docking studies using Discovery Studio (DS) 4.0. The drug parallel features of the same compounds were also assessed in silico. Results: Cytotoxicity experiments revealed that both the compounds were efficient in killing CRC cells on a controlled concentration basis. In addition, EGFR expression was down-regulated in the presence of the compounds. Docking studies unveiled that both the compounds were able to inhibit EGFR at its active site. Pharmacokinetic analysis of these compounds opened up their drug like behaviour. Conclusions: The findings of this study emphasize the importance of plant compounds for targeting diseases like CRC.

• 가족자원경영과 정책
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• 제9권3호
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• pp.31-43
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• 2005

The purposes of this study were to (1) examine the sub-dimensions of time management behaviors and satisfaction with time management, and (2) to identify the variables that can affect satisfaction with time management. Data was collected through an Internet survey answered by 611 working men and women. The data were analyzed using descriptive statistics, factor analysis, and multiple linear regression analysis. As a result of the study, the sub-dimensions of time management behaviour were extracted into three subordinate categories, which were, planning, human relation orientation, and urgency. The sub-dimensions of the satisfaction with time management were extracted into three subordinate categories, which were, satisfaction with the management results, satisfaction with time distribution, and satisfaction of spare time. Management result satisfaction displayed a positive relationship with planning and work time. The satisfaction with time distribution displayed a positively relationship with planning, relations-intention, the level of education, and sleeping time, while displaying a negative relationship with work time. Also, Women tended to be more satisfied with time distribution than men. Finally, the satisfaction of spare time was positively related with age, while negatively related to urgency and work time.

• Advances in Computational Design
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• 제3권4호
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• pp.339-366
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• 2018

Parametric design systems serve as powerful assistive tools in the design process by providing a flexible approach for the generation of a vast number of design alternatives. However, contemporary parametric design systems focus primarily on low-level engineering and structural forms, without an explicit means to also take into account high-level, cognitively motivated people-centred design goals. We present a precedent-based parametric design method that integrates people-centred design "precedents" rooted in empirical evidence directly within state of the art parametric design systems. As a use-case, we illustrate the general method in the context of an empirical study focusing on the multi-modal analysis of wayfinding behaviour in two large-scale healthcare environments. With this use-case, we demonstrate the manner in which: (1). a range of empirically established design precedents -e.g., pertaining to visibility and navigation- may be articulated as design constraints to be embedded directly within state of the art parametric design tools (e.g., Grasshopper); and (2). embedded design precedents lead to the (parametric) generation of a number of morphologies that satisfy people-centred design criteria (in this case, pertaining to wayfinding). Our research presents an exemplar for the integration of cognitively motivated design goals with parametric design-space exploration methods. We posit that this opens-up a range of technological challenges for the engineering and development of next-generation computer aided architecture design systems.

• 해양환경안전학회지
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• 제12권1호
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• pp.39-45
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• 2006

항공기와 자동차의 운전을 중심으로 알코올이 인간의 행동과 수행도에 미치는 연구가 많이 이루어져 왔으며 이를 통해 선박의 당직 전 및 당직 중의 음주는 이어지는 행동에 큰 영향을 미친다고 할 수 있다. 본 연구에서는 승선 중 음주 실태를 파악하기 위하여, 선택형 앙케트를 교육중인 사관들에게 배포하여 118명으로부터 얻어진 결과를 조사하였다. 알코올 의존도에 관한 자가진단결과에 따르면, 조사대상자의 27% 이상이 알코올 남용 증세를 나타내었다. 또한, 승선 중 음주 현황 및 인식에 관한 사항을 정리하여, 선박운항 시뮬레이터를 이용한 음주 운항 시나리오를 마련하였으며, 알코올이 선박운항능력에 미치는 영향을 알아보았다. 실험결과를 분석한 결과, 알코올의 섭취가 선박운항능력을 다소 저하시키는 것으로 나타났다. 이러한 분석결과는 앞으로 선박운항자의 피로에 의한 해양사고 발생을 근본적으로 줄이기 위한 기초자료로 활용할 수 있을 것이다.

• 한국수학교육학회지시리즈E:수학교육논문집
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• 제37권1호
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• pp.21-45
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• 2023

본 연구는 수학교육에서 영향력 있는 논문을 판별하는 기계학습 프로그램 개발 연구이다. 이를 위하여 과학계량학의 관점에서 논문의 영향력을 조명하고, 수학교육 연구 네트워크를 구성하고, 네트워크 중심성 지수인 PageRank로 수학교육 연구의 영향력으로 정의하였다. 영향력 있는 수학교육 연구를 판별하기 위하여 기계학습 모델을 설계하였으며, 이를 이용하여 영향력 있는 논문이 게재된 비율이 높은 학술지를 조사한 결과 Journal for Research in Mathematics Education(25.66%), Educational Studies in Mathematics(22.12%), Zentralblatt für Didaktik der Mathematik(8.46%), Journal of Mathematics Teacher Education(5.8%), Journal of Mathematical Behavior(5.51%) 순으로 나타났다. 수학교육 전문가들이 직접 논문을 읽고 질적으로 평가한 선행연구 결과와 유사한 결과를 기계학습 프로그램으로 도출할 수 있었다. 많은 인원과 시간이 필요했던 수학교육 연구의 영향력 평가를 인공지능을 이용하여 효율적으로 실시할 수 있었다는 점에서 의의가 있다.

• International Journal of Human Ecology
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• 제2권1호
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• pp.57-75
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• 2001

Since the Korean economic crisis in 1997, consumers are selecting goods that are more economical and more reasonably priced. Markets for wealthy people, however, have seldom been influenced by economic conditions. Consequently, 'noble marketing' is a new term that marketers should become acquainted with (Kim, 1998). The continuously expanding 'noble market' that generates high profit for marketers needs to be explored, especially the men's wear market. This study adopted a qualitative method (i.e., in-depth interview) with 16 male consumers each. These men who spent more than 3,600,000 won on apparel goods in 2000 and each man had purchased at least one item-suit, jacket, trousers, or coat-from a list of high-priced brands that were selected by the researcher. Findings imply that material or functional benefit of the high-priced apparel were not a critical determinant for choosing the brand. Rather, a socially well-established image for the brand name, especially in terms of psychological/symbolic appeal, played a more important role. This finding supported previous studies. Therefore, for high-priced men s wear, the role of promotion, including advertising or word of mouth, is critical; the image that advertisers should promote is one that characterizes the clothing, and ultimately the wearers, as dignified, legitimate, traditional, and upper class.

• 통합자연과학논문집
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• 제9권3호
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• pp.166-170
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• 2016

Collagen plays a vital role in the maintenance of structure and function of a human body. It has been widely applied in various fields including biomedical, cosmeceutical, food, pharmaceutical and tissue engineering. In the present study, the docking behaviour of type I collagen with 15 different ligands namely hydroxymethylfurfural, methylglyoxal, methylsyringate, O-methoxyacetophenone, 3-phenyllactic acid, 4-hydroxybenzoic acid, kojic acid, lumichrome, galangin, artoindonesianin F, caffeic acid, 4-coumaric acid, origanol A, thymoquinone and quercetin was evaluated along with their putative binding sites using Discovery Studio Version 3.1. Docking studies and binding free energy calculations revealed that origanol A has maximum interaction energy (-40.48 kcal/mol) and quercetin with the least interaction energy (-15.44 kcal/mol) as compared to the other investigated ligands. Three ligands which are galangin, methylsyringate and origanol A were shown to interact with Asp21 amino acid residue of chain B (type I collagen). Therefore, it is strongly suggested that the outcomes from the present study might provide new insight in understanding these 15 ligands as potential type I collagen modulators for the prevention of collagen associate disorders.

• 한국생활과학회지
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• 제10권1호
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• pp.113-126
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• 2001

The purpose of this study was to investigate clothing behavior and ornaments wear of university students. The subject of this study consists of 241 college students who were attended in Kongju National University. SPSSWIN Program was utilized to calculated frequency(N), mean value(M) and standard deviation(SD) for statistical analysis. Differences among the populations were examined through T-test, F-test, $x^2$-test. The results of this study were as follow : 1. Clothing behavior of university students had significant difference according to sex, grade. In general, female students showed higher score than did male students in clothing interest, clothing conformity, self-estimation about clothing, clothing dependence, clothing aesthetic, clothing modesty, A first-year students had high tendency in clothing interest. 2. The wear of ornaments had significant difference according to variables. Buying the ornaments, most of students sot information in show window and emphasized on design. Male students wanted to have the ornaments of shoes, watch, hat & necklet, but female students wanted to have the ornaments of shoes, earring, necklet & hairpin. 3. Clothing behavior has Influenced university students' ornaments wear. The university students who appeared to be interested in clothes were apparently influenced by peers when selecting ornaments. Ornaments were usually bought in the department store, while belts were among the most popular choices. Students who stressed color when purchasing ornaments showed a high level of interest in and dependence on clothes. On the basic of these results, we can conclude that university students' taste in clothing behavior and use of ornaments wear depend on several different factors. Therefore, it is possible to outline the shift in clothing attitudes during adolescence. In order to understand university students' behaviour concerning clothes, much guidance and various studies on the subject are much recommended.

• Asian Pacific Journal of Cancer Prevention
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• 제16권9호
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• pp.3817-3825
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• 2015

Background: The human protein methyl-transferase DOT1L catalyzes the methylation of histone H3 on lysine 79 (H3K79) at homeobox genes and is also involved in a number of significant processes ranging from gene expression to DNA-damage response and cell cycle progression. Inhibition of DOT1L activity by shRNA or small-molecule inhibitors has been established to prevent proliferation of various MLL-rearranged leukemia cells in vitro, establishing DOT1L an attractive therapeutic target for mixed lineage leukemia (MLL). Most of the drugs currently in use for the MLL treatment are reported to have low efficacy, hence this study focused on various natural compounds which exhibit minimal toxic effects and high efficacy for the target receptor. Materials and Methods: Structures of human protein methyl-transferase DOT1L and natural compound databases were downloaded from various sources. Virtual screening, molecular docking, dynamics simulation and drug likeness studies were performed for those natural compounds to evaluate and analyze their anti-cancer activity. Results: The top five screened compounds possessing good binding affinity were identified as potential high affinity inhibitors against DOT1L's active site. The top ranking molecule amongst the screened ligands had a Glide g-score of -10.940 kcal/mol and Glide e-model score of -86.011 with 5 hydrogen bonds and 12 hydrophobic contacts. This ligand's behaviour also showed consistency during the simulation of protein-ligand complex for 20000 ps, which is indicative of its stability in the receptor pocket. Conclusions: The ligand obtained out of this screening study can be considered as a potential inhibitor for DOT1L and further can be treated as a lead for the drug designing pipeline.