• Journal of Digital Contents Society
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• v.15 no.4
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• pp.449-455
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• 2014

Detection of moving objects is a fundamental task in most of the computer vision applications, such as video surveillance, activity recognition and human motion analysis. This is a difficult task due to many challenges in realistic scenarios which include irregular motion in background, illumination changes, objects cast shadows, changes in scene geometry and noise, etc. In this paper, we propose an foreground extraction algorithm based on codebook, a database of information about background pixel obtained from input image sequence. Initially, we suppose a first frame as a background image and calculate difference between next input image and it to detect moving objects. The resulting difference image may contain noises as well as pure moving objects. Second, we investigate a codebook with color and brightness of a foreground pixel in the difference image. If it is matched, it is decided as a fault detected pixel and deleted from foreground. Finally, a background image is updated to process next input frame iteratively. Some pixels are estimated by input image if they are detected as background pixels. The others are duplicated from the previous background image. We apply out algorithm to PETS2009 data and compare the results with those of GMM and standard codebook algorithms.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2015.05a
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• pp.958-961
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• 2015

Emotional expression is universal and emotional state impacts important areas in our life. Until now, analyzing the acquired EEG signals under circumstances caused by invoked feelings and efforts to define their emotional state have been made mainly by psychologists based on the results. But, recently emotion-related information was released by research results that it is possible to identify mental activity through measuring and analyzing the brain EEG signals. So, this study has compared and analyzed emotional expressions of human by using brain waves. To get EEG difference for a particular emotion, we showed specific subject images to the people for changing emotions that peace, joy, sadness and stress, etc. After measured EEG signals were converged into frequence domain by FFT signal process, we have showed EEG changes in emotion as a result of the performance analyzing each respective power spectrum of delta, theta, alpha, beta and gamma waves.

• Journal of Environmental Science International
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• v.18 no.11
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• pp.1199-1206
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• 2009

Obesity affects various kinds of adult diseases and is a principal cause of injuring people's health. Physical activities are very important to solve the obesity problem and green spaces are good place to take physical activities. In order to examine the utilization of green spaces for physical activities to improve health, the survey was implemented in regards to recognition of health, forms of use of green spaces for physical activities. Only 8.1% of respondents didn't care about health, but 91.9% of them were interested in health more than 'commonness'. 95.2% of respondents knew that obesity has an effect on various adult diseases, and 93.1% of respondents cognized that it's possible to prevent obesity by doing physical activities. More than half of respondents replied that the types of green spaces utilized for physical activities were small-scale neighborhood parks and mini parks around house. About 70% of respondents took walking as a type of physical activities. 53% of respondents utilized green spaces one or two times a week, while 25% of them utilized the places at least three times a week to take physical activities. 91.8% of respondents took exercise for more than 30 minutes. These results are expected to promote health because many experts encourage people to take physical activities at least three times a week because fats of human body begins to bum after 30 minutes from start of physical activities. In the utilizing time of green spaces, evening or after work(56.7%) was higher than morning or before work(35.1%). 71% of respondents usually visited green spaces by walking. This study shows green spaces are good places for physical activities and have boundless potentialities improving people's health.

• Journal of the Institute of Convergence Signal Processing
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• v.8 no.3
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• pp.178-184
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• 2007

The EEG signal in general can be categorized as the Alpha wave, the Beta wave, the Theta wave, and the Delta wave. The alpha wave, showed in stable state, is the dominant wave for a human EEG and the beta wave displays the excited state. The subject of this paper was to recognize the stable state of EEG quantitatively using wavelet transform and power spectrum analysis. We decomposed EEG signal into the alpha wave and the beta wave in the process of wavelet transform, and calculated each power spectrum of EEG signal, using Fast Fourier Transform. And then we calculated the stable state quantitatively by stable state ratio, defined as the power spectrum of the alpha wave over that of the beta wave. The study showed that it took more than 10 minutes to reach the stable state from the normal activity in 69 % of the subjects, 5 -10 minutes in 9%, and less than 5 minutes in 16 %.

• Journal of Microbiology and Biotechnology
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• v.23 no.7
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• pp.1031-1040
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• 2013

Candida albicans is a dimorphic fungus that commensally colonizes human mucosal surfaces. The aim of this study was to assess the role of different C. albicans morphologies in inducing pattern recognition receptors (PRRs) and cytokines in macrophages. Macrophages may respond to pathogen-associated molecular patterns via TLR2 and TLR4 by expressing cytokines. The hyphal transition of C. albicans was induced by 20% serum (S), RPMI-1640 (R), or $39^{\circ}C$ culture (H). Macrophages were then challenged with either yeast (Y) or different hyphae cultures of C. albicans, followed by RT-PCR and FACS analysis of PRRs expression. In addition, macrophages were stimulated with either yeast or different hyphae cultures of C. albicans used by RT-PCR and Bio-Plex analysis of cytokines production. Macrophages expressed high levels of TLR4 and dectin-1 after stimulation with Y cells. In contrast, stimulation with H or R cells strongly increased the expression of TLR2 and dectin-2. Stimulation with Y cells significantly enhanced the expression of IL-$1{\beta}$ and weakly increased the expression of IL-6 and IL-12. Stimulation with hyphal cells (S, R, and H) strongly increased IL-10 expression, but weakly reduced IL-$1{\beta}$ expression. The phagocytosis activity and NO production of macrophages were decreased upon treatment with hyphal cells compared with yeast, and depended on the length of hyphae. In summary, the yeast and hyphae forms of C. albicans resulted in an induction of different PRRs, with accompanying differences in immune cell cytokine profiles.

• Journal of the Korean Geographical Society
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• v.44 no.3
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• pp.372-394
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• 2009

This study investigates the ontology-based public participation GIS(PPGIS). The major reason that ontology-based GIS has attracted attention in semantic communication in recent year is due to the wide availability of geographical variable and the imminent need for turning such recommendation into useful geographical knowledge. Therefore, this study has been focused on designing and implementing the pilot tested system for public participation GIS. The applicability of the pilot tested was validated through a simulation experiment for history tourism in Guri city Gyeongi-do, Focused on the methodology, the life cycle model which involves regional statues and user recognition, can be viewed as an important preprocessing step(specification, conceptualization, formalization, integration and implementation) for recommended geographical knowledge discovery by axiom. Focusing on practicality, ontology in this study would be recommended for geographical knowledge through reasoning. In addition, ontology-based public participation GIS would show integration epistemological and ontological approach, and be utilized as an index which is connected with semantic communication. The results of the pilot system was applied to the study area, which was a part of scenario. The model was carried out using axiom of logical constraint in the meaning of human-activity.

• International Journal of Computer Science & Network Security
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• v.23 no.11
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• pp.142-148
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• 2023

The National Agency for Quality Assurance in Higher Education plays a crucial role in education in Ukraine, as an independent entity creates and ensures quality standards of higher education, which allow to properly implement the educational policy of the state, develop the economy and society as a whole.The purpose of the article: to reveal the crucial role of the National Agency for Quality Assurance in Higher Education to create quality management of higher education institutions, to show its mechanism as an independent entity that creates and ensures quality standards of higher education. and society as a whole. The mission of the National Agency for Quality Assurance in Higher Education is to become a catalyst for positive changes in higher education and the formation of a culture of its quality. The strategic goals of the National Agency are implemented in three main areas: the quality of educational services, recognition of the quality of scientific results, ensuring the systemic impact of the National Agency. The National Agency for Quality Assurance in Higher Education exercises various powers, which can be divided into: regulatory, analytical, accreditation, control, communication.The effectiveness of the work of the National Agency for Quality Assurance in Higher Education for 2020 has been proved. The results of a survey conducted by 183 higher education institutions of Ukraine conducted by the National Agency for Quality Assurance in Higher Education are shown. Emphasis was placed on the development of "Recommendations of the National Agency for Quality Assurance in Higher Education regarding the introduction of an internal quality assurance system." The international activity and international recognition of the National Agency for Quality Assurance in Higher Education are shown.

• Journal of Embryo Transfer
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• v.31 no.4
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• pp.335-347
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• 2016

Multiple interferon tau (IFNT) genes exist in bovine. An antiluteolytic substance secreted by the bovine conceptus and primarily responsible for maternal recognition of pregnancy is bovine trophoblast protein 1 (bIFNT1), a new type I interferon tau (IFNT) genes. The objectives of this research were to investigate whether multiple, distinct gene encode bIFNT1 and other type I bIFNT gene in the bovine genome and to examine expression of bIFNT1 and other bIFNTc1 mRNAs during conceptus development. These transcrips could be regulated through caudal-related homeobox-2 (CDX2) and ETS2 and/or AP1 (JUN) expression, a transcription factor implicated in the control of cell differentiation in the trophectoderm. The presence of mRNAs encoded by bIFNT1 and type I bIFNTc1 genes were examined quantitatively via reverse transcription-polymerase chain reaction (RT-PCR) analysis of total cellular RNA (tcRNA) extracted from on day 17, 20 and 22 bovine conceptuses. The expression level of bIFNT1 was higher on day 17 transcripts were gradually weakly detectable on day 20 and 22. However, the other bIFNTc1 gene examined transcripts was highly expressed on day 20 and transcripts were weakly detectable on day 17 and 22 bovine conceptuses. Furthermore, human choriocarcinoma JEG3 was co-transfected with an -1kb-bIFNT1/c1-Luc constructs and several transcription factor expression plasmids. Compared to each -1kb-bIFNT1/c1-Luc increased when this constructs were co-transfected with, ETS2, AP1(JUN), CREBBP and/or CDX2. Also, bIFNTc1 gene was had very effect on activity by alone ETS2, and AP1 (JUN) expression factors in choriocarcinoma JEG3 cell. However, bIFNT1 gene expression of the upstream region was not identified. We demonstrated that the activities of bIFN genes are regulated by differential, tissue-specific and developmental competence during pregnancy.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.5
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• pp.638-645
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• 2013

Interferon-tau (IFNT) is thought to be the conceptus protein that signals maternal recognition of pregnancy in ruminants. We and others have observed that OCT4 expression persists in the trophectoderm of ruminants; thus, both CDX2 and OCT4 coexist during the early stages of conceptus development. The aim of this study was to examine the effect of CDX2 and OCT4 on IFNT gene transcription when evaluated with other transcription factors. Human choriocarcinoma JEG-3 cells were cotransfected with an ovine IFNT (-654-bp)-luciferase reporter (-654-IFNT-Luc) construct and several transcription factor expression plasmids. Cotransfection of the reporter construct with Cdx2, Ets2 and Jun increased transcription of -654-IFNT-Luc by about 12-fold compared with transfection of the construct alone. When cells were initially transfected with Oct4 (0 h) followed by transfection with Cdx2, Ets2 and/or Jun 24 h later, the expression of -654-IFNT-Luc was reduced to control levels. OCT4 also inhibited the stimulatory activity of CDX2 alone, but not when CDX2 was combined with JUN and/or ETS2. Thus, when combined with the other transcription factors, OCT4 exhibited little inhibitory activity towards CDX2. An inhibitor of the transcriptional coactivator CREB binding protein (CREBBP), 12S E1A, reduced CDX2/ETS2/JUN stimulated -654-IFNT-Luc expression by about 40%, indicating that the formation of an appropriate transcription factor complex is required for maximum expression. In conclusion, the presence of OCT4 may initially minimize IFNT expression; however, as elongation proceeds, the increasing expression of CDX2 and formation of the transcription complex leads to greatly increased IFNT expression, resulting in pregnancy establishment in ruminants.

• Journal of Life Science
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• v.21 no.1
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• pp.119-126
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• 2011

There is a growing recognition of the significance of $H_2S$ as a biological signaling molecule involved in vascular and nervous system functions. In mammals, two enzymes in the transsulfuration pathway, cystathionine ${\beta}$-synthase (CBS) and cystathionine ${\gamma}$-lyase (CGL), are believed to be chiefly responsible for $H_2S$ biogenesis. Genetic inborn error of CGL leads to human genetic disease, cystathioninuria, by accumulating cystathionine in the body. This disease is secondarily associated with a wide range of diseases including diabetes insipidus and Down's syndrome. Although the human CGL (hCGL) overexpression is essential for the investigation of its function, structure, reaction specificity, substrate specificity, and protein-protein interactions, there is no clear report concerning optimum overexpression conditions. In this study, we report a detailed analysis of the overexpression conditions of the hCGL using a bacterial system. Maximum overexpression was obtained in conditions of low culture temperature after inducer addition, performing low aeration during overexpression, and using a low concentration inducer (0.1 mM, IPTG) for induction. Expressed hCGL was purified by His-tag affinity column chromatography and confirmed by Western blot using hCGL antibody and enzyme activity analysis. We also report that the His tag with TEV site attached protein exhibits 76% activity for ${\alpha}-{\gamma}$ elimination reaction with L-cystathionine and 88% for ${\alpha}-{\beta}$ elimination reaction with L-cysteine compared to those of wild type hCGL, respectively. His tag with TEV site attached protein also exhibits a 420 nm absorption maximum, which is attributed to the binding cofactor, pyridoxal 5'-phosphate (PLP).