• Journal of Applied Biological Chemistry
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• 제52권4호
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• pp.221-225
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• 2009

Lithospermum erythrorhizon has been known well as one of traditional medicine for fever reduction, detoxication, and blood circulation improvement. This study was carried out to isolate biological active compounds from roots of Lithospermum erythrorhizon and to investigate their low density lipoprotein (LDL) antioxidant and anticancer activities. The hexane extract of Lithospermi Radix has been separated on silica gel chromatography and a naphthoquinone pigment compound 1 has been isolated. The structure of the compound 1 has been identified by spectroscopic technique, including MS and NMR, as acetylshikonin (1). Acetylshikonin showed significantly inhibitory activity on $Cu^{2+}$-induced human LDL oxidation with $IC_{50}$ value of $8.8\;{\mu}M$ and obvious anticancer effect by inhibiting farnesyl:protein transferase (FPTase) activity with $IC_{50}$ value of $23\;{\mu}M$, which suggested that acetylshikonin might be useful for the treatment of atherosclerosis and cancer.

• Mycobiology
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• 제39권4호
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• pp.283-289
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• 2011

The wild edible mushroom, Lentinus lepideus has recently been cultivated for commercial use in Korea. While the mushroom has been widely used for nutritional and medicinal purposes, the possible anti-hyperlipidemic action is unclear. The effects of dietary L. lepideus on plasma and feces biochemical and on the liver histological status were investigated in hypercholesterolemic rats. Six-wk-old female Sprague-Dawley albino rats were divided into three groups of 10 rats each. Biochemical and histological examinations were performed. A diet containing 5% L. lepideus fruiting bodies reduced plasma total cholesterol, triglyceride, low-density lipoprotein, total lipid, phospholipids, and the ratio of low-density to high-density lipoprotein. Body weight was reduced. The diet did not adversely affect plasma biochemical and enzyme profiles. L. lepideus reduced significantly plasma ${\beta}$- and pre-${\beta}$-lipoprotein, while ${\alpha}$-lipoprotein content was increased. A histological study of hepatic cells by conventional hematoxylin-eosin and oil red O staining revealed normal findings for mushroom-fed hypercholesterolemic rats. The present study suggests that a diet supplemented with L. lepideus can provide health benefits by acting on the atherogenic lipid profile in hypercholesterolemic rats.

• 한국자원식물학회지
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• 제27권3호
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• pp.215-222
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• 2014

This study was conducted to isolate a compound with anticancer properties from the roots of Peucedanum japonicum Thunb. (Umbelliferae), and to evaluate the efficacy of that compound's anticancer activity. The $CHCl_3$ layer was purified via repeated column chromatography and recrystallization. The two compounds isolated from $CHCl_3$ layer were identified via NMR spectroscopic analysis as (10E) 1,10-heptadecadiene-4,6-diyne-3,8,9-triol (Comp. I) and anomalin (Comp. II). (10E) 1,10-heptadecadiene-4,6-diyne-3,8,9-triol was the first report from the roots of P. japonicum. MTT assays were conducted to evaluate the in vitro cytotoxic activities of Compounds I and II against the following human cancer cell lines: HeLa, HepG2, SNU-16, and AGS. Comp. I evidenced the most profound cytotoxic activity against HepG2 cells ($IC_{50}=6.04{\mu}g/mL$), and Comp. II exhibited the most profound cytotoxic activity against SNU-16 cells ($IC_{50}=18.24{\mu}g/mL$) among the human cancer cell lines tested in this study. However, no significant cell death was observed in the CCD-25Lu human normal lung fibroblast cells. Quantitative analysis using UPLC (Ultra Performance Liquid Chromatography) showed that the roots of P. japonicum contained 0.015 (Comp. I) and 1.69 mg/g (Comp. II) of these compounds.

• 인간식물환경학회지
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• 제23권1호
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• pp.67-75
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• 2020

The purpose of this study was to develop an effective horticultural activity program for the vocational rehabilitation of people with mental disabilities. The subjects of this study were seven persons with mental disabilities in the vocational rehabilitation team of a public health center located in G city, Gyeongsangbuk-do. A total of 10 horticultural programs were held from October 10 to December 11, 2018. Five programs were selected among those that were easy for the persons with mental illness to handle and their preference was high. In each session, the same task was repeated 10 times and finally changes in hand functions and working speed were measured. As a result, the assembly task of the Purdue Pegboard test was 23.6 (SD = 10.5) before the program, 26.6 (SD = 11.5) after planting, 27.7 (SD = 12.2) after wrapping pots, and 28.0 (SD = 13.9) after making mini flower baskets, 26.9 (SD = 12.0) after wrapping a single flower, and 29.1 (SD = 11.9) after making corsages. There was a significant difference between the five programs (p = .016). As a result of measuring the time taken to produce the same 10 products, the average planting time was 9.9 (SD = 4.0) minutes in the first class and 6.0 (SD = 2.3) minutes in the second class (p = .018), and making mini flower baskets was reduced from 35.2 (SD = 10.1) minutes to 21.0 (SD = 7.7) minutes (p = .018), wrapping a single flower from 23.3 (SD = 7.9) minutes to 16.2 (SD = 7.3) minutes (p = .043), and making corsages from 53.6 (SD = 15.9) to 40.8 (SD = 16.8) minutes (p = .043). The working time was significantly shortened in the second class compared to the first class. The class for wrapping pots was shortened from 52.7 (SD = 11.7) to 49.6 (SD = 17.8) minutes, but there was no significant difference (p = .398).

• 한국환경보건학회지
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• 제49권6호
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• pp.295-304
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• 2023

Background: Adolescents are relatively more sensitive than adults to exposure to indoor pollutants. The indoor air quality in classrooms where students spend time together must therefore be managed at a safe level because it can affect the health of students. Objectives: In this study, three types of green-wall models were applied to classrooms where students spend a long time in a limited space, and the resulting effects on reducing PM were evaluated. Methods: In the middle school classrooms which were selected as the experimental subjects, IoT-based indoor air quality monitoring equipment was installed for real-time monitoring. Three types of plant models (passive, active, and active+light) were installed in each classroom to evaluate the effects on improving indoor air quality. Results: The concentration of PM in the classroom is influenced by outdoor air quality, but repeated increases and decreases in concentration were observed due to the influence of students' activities. There was a PM reduction effect by applying the green-wall model. There was a difference in PM reduction efficiency depending on the type of green-wall model, and the reduction efficiency of the active model was higher than the passive model. Conclusions: The active green-wall model can be used as an efficient method of improving indoor air quality. Additionally, more research is needed to increase the efficiency of improving indoor air quality by setting conditions that can stimulate the growth of each type of plant.

• 원예과학기술지
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• 제35권1호
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• pp.88-97
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• 2017

This study explored the physicochemical and nutritional changes associated with storage duration of fresh tomatoes. Fruits of the 'TY Megaton' and 'Yureka' tomato cultivars were harvested at the pink stage and stored at $12^{\circ}C$ for 20 days. During storage, firmness, weight loss, skin color (Hunter L, a, b, a / b values), soluble solids content (SSC), titratable acidity (TA), pH, antioxidant contents (lycopene, ascorbic acid, and total phenolics) and antioxidant activity were evaluated. Firmness was above the minimum marketable limit and fresh weight loss was below maximum acceptable weight loss after 3 weeks of storage, and no deleterious effect on antioxidant contents or activities were observed. Significant differences in SSC, TA, and pH were seen between varieties, but not between fruits stored for different durations. In both varieties, Hunter a values increased more than five-fold after 8 days of storage; this correlated with a more than four-fold accumulation of lycopene after two weeks of storage. The antioxidant activity of tomatoes was highest at the beginning of the storage period, likely because of the effective DPPH - reducing power of ascorbic acid and total phenolics. Antioxidant activity increased after 12 days of storage because of increasing lycopene content. Hence, this study indicates that pink - stage tomatoes may be stored at $12^{\circ}C$ for up to 3 weeks without affecting marketability or nutritional value.

• 인간식물환경학회지
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• 제23권2호
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• pp.125-138
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• 2020

The aim of this study was to review previous literature to determine the effects of nature-based program for workers. This systematic review was conducted in accordance with National Evidence-based Healthcare Collaborating Agency's guidance for undertaking systematic reviews for intervention. Literature search was performed using National Assembly Digital Library, Korean Studies Information Service System, and Korea Education & Research Information Service for literature published until March 2019. The participants were full-time workers, and intervention of nature-based programs was conducted in the outdoor, indoor, and indirect nature contact exposures, with comparators in the control group who did not receive the treatment. The results showed that the programs were effective in physical, psychological, and social health. The methodological quality of randomized controlled trials (RCT) was assessed using the Cochrane Risk of Bias(RoB) tool, while non-randomized controlled trials (N-RCT) were assessed using the Cochrane Risk of bias assessment tool for non-randomized studuies (RoBANS). A total of 16 studies were selected for assessment: two RCTs, 10 N-RCTs, and four one-group pretest-posttest designs. Most interventions were provided at the workplace and in the community. There were many kinds of nature-based interventions, and forest therapy and horticultural therapy programs were most common. Various interventions for workers effectively improved job stress, depression, serum cortisol and stress-response. However, the included studies lacked methodological rigor. Future research is needed to evaluate the long-term effectiveness of nature-based programs for workers using rigorous research designs.

• 한국식품저장유통학회지
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• 제23권2호
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• pp.174-179
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• 2016

배의 가공 활용을 위해 숙기가 다른 원황, 신고 및 추황배를 이용하여 식초를 제조하여 이화학적 특성 및 항산화활성을 조사하였다. 숙기가 다른 배의 특성 조사 결과 경도는 숙기가 가까운 원황은 낮았고 미숙과인 신고, 추황배는 높았으며, 당도는 유의적인 차이가 없었다. 품종별 배식초의 품질 특성을 조사한 결과 총 산 함량은 4.89~7.98%로 추황배가 가장 높았고, 색도 $L^*$ 값은 원황 신고 추황배를 이용하여 제조한 식초의 색도 $L^*$ 값은 유의적인 차이를 나타내지 않았고, 적색도 $a^*$ 값은 원황 배를 이용하여 제조한 식초가 가장 높았으며, 황색도 $b^*$ 값은 신고배를 이용하여 제조한 식초가 가장 높은 값을 나타내었다. 주요 유리당은 glucose, fructose, sorbitol로 품종에 관계없이 sorbitol의 함량이 가장 높았다. 총 폴리페놀 및 플라보노이드 함량은 35.2~55.3, 8.4~14.4 mg/100 g으로 추황배를 이용하여 제조한 식초가 높았고, 원황, 신고를 이용하여 제조한 식초가 낮았다. 항산화 활성은 총페놀과 정의 상관을 보였으며, DPPH 라디컬 소거능은 세가지 품종 배를 이용하여 제조한 식가 45.0~62.1%였고, $ABTS^+$ radical 소거능은 73.8~78.2%를 나타내었다. 이상의 결과로부터 낙과나 비 상품과인 원황, 신고 및 추황배를 사용하여 기호성을 갖춘 식초를 제조할 수 있는 것으로 판단되어 이에 대한 관능적 특성 및 대규모 생산에 대한 추후 연구가 필요할 것으로 사료된다.

• 생명과학회지
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• 제24권3호
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• pp.252-259
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• 2014

머위(Peatasites japonicus)의 잎으로부터 생리활성물질을 탐색하여 항천식 효과가 있는 물질로 알려진 bakenolide B를 순수분리하였으며, 1D-NMR, 2D-NMR 및 GC-MS spectrum 데이터를 이용하여 구조를 동정하였다. 머위의 잎, 엽병 및 근경 등의 채취시기별 bakkenolide B의 함량을 평가하기 위하여 HPLC 분석 방법을 확립하였다. Bakkenolide B의 민감도는 210 nm와 215 nm의 파장에서 254 nm, 235 nm 및 265 nm보다 높았으며, 분석을 위한 회귀 직선식은 y=7.841-6.262(파장 215 nm)로서 상관값($r^2$)이 0.999 이상으로서 유의성이 매우 높았다. 검출한계(LOD)는 1.05, 정량한계(LOQ)는 3.38이었다. 회수율은 3종류의 농도값에 대하여 98.6에서 103.1%로서 매우 높았다. 이러한 결과들로 미루어 볼때 머위에서 bakkenolide B의 함량은 HPLC 분석방법으로서 가능한 것으로 증명되었다. 따라서 식물체의 부위 즉 잎, 엽병 및 근경에서의 bakkenolide B의 함량을 측정한 결과, 잎에서 가장 높았고, 다음은 엽병이었으며, 근경에서 함량이 가장 낮았다. 3월 초부터 8월 초까지 1개월 간격으로 식물체의 부위별 bakkenolide B의 함량을 조사한 결과, 3월부터 8월까지 채취시기가 지연될수록 감소하는 경향이었다. 따라서 HPLC를 이용한 Petasites species의 bakkenolides 분석은 단순하고, 급속하게 분석할 수 있는 방법으로 확인되었다.

• KSBB Journal
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• 제15권3호
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• pp.226-231
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• 2000

원예산물의 저온저장중 부패방지를 위한 천연항균제 개발의 일환으로 식용 가능한 식물을 채집하여 E. coli K 12, B. subtilis K KCTC 1028 및 딸기부패균에 대한 항균활성을 조사하였다. 사철 쑥, 가죽나무 잎, 초피 잎, 적송 잎, 뽕 잎, 씀바귀, 마늘의 즙액은 E. coli와 B. subtilis에 대하여 높은 항균활성을 보였다. 이들 식물체 즙액으로부터 n-hexane, ethyl ace떠te, chloroform 및 물로 용매분획한 분획물로 항균성을 재검색한 결과, 사철쑥, 가죽나무 잎, 적송 잎, 씀바귀의 ethyl acetate 분획과 초피 잎과 마늘의 모 든 분획에서 비교적 높은 향균활성을 보였으며 가죽나무 잎의 ethyl acetate 분획 이 가장 높은 항균활성(6 nun)을 보였다. 한편 딸기의 저온저장중인 과육으로부터 두 종의 부패 세균을 분리하 여 각각 Staphylococcus sp. TG-IOI과 Staphylococcus sp. TG-I02 로 동정하였다. 이들 균주에 대한 항균성을 조사한 결과, 가죽나 무 잎의 ethyl acetate 분획에서 두 균주에 대하여 가장 높은 저 해효과(각각 6 mm, 7 nun)를 보였다.