• Journal of the Chungcheong Mathematical Society
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• v.31 no.2
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• pp.239-246
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• 2018

In this paper, we define a concept of weak co-T-cofibration which is a generalization of weak H'-cofibration, and study some properties of weak co-T-cofibration and relations between the weak co-T-cofibration and the homology decomposition for a cofibration.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.20 no.9
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• pp.2800-2811
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• 1996

The deformation of a structure shall be called homologous, if a given geometrical relation holds, for a given number of structural points, before, during, and after the deformation. Some researchers have utilized the idea on structural design with finite element method. The approaches use the decomposition of the FEM equation or equality of eqality equations to obtain homologous deformation. However, weight reduction and response constraints such as stress, displacement or natural frequency cannot be considered by those theories. An optimization method solving the above problems is suggested to gain homologous deformation. Homology constraints can be considered under multiple loadindg conditions as well as a single loading condition. Homology index is defined for the multiple loading conditions Examples are solved to present the performances of the method.

• Journal of Microbiology and Biotechnology
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• v.34 no.9
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• pp.1867-1875
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• 2024

Identification of the biochemical metabolic pathway for lignin decomposition and the responsible degradative enzymes is needed for the effective biotechnological valorization of lignin to renewable chemical products. In this study, we investigated the decomposition of kraft lignin by the soil bacterium Pseudomonas kribbensis CHA-19, a strain that can utilize kraft lignin and its main degradation metabolite, vanillic acid, as growth substrates. Gel permeation chromatography revealed that CHA-19 decomposed polymeric lignin and degraded dehydrodivanillin (a representative lignin model compound); however, the degradative enzyme(s) and mechanism were not identified. Quantitative polymerase chain reaction with mRNAs from CHA-19 cells induced in the presence of lignin showed that the putative genes coding for two laccase-like multicopper oxidases (LMCOs) and three dye-decolorizing peroxidases (DyPs) were upregulated by 2.0- to 7.9-fold compared with glucose-induced cells, which indicates possible cooperation with multiple enzymes for lignin decomposition. Computational homology analysis of the protein sequences of LMCOs and DyPs also predicted their roles in lignin decomposition. Based on the above data, CHA-19 appears to initiate oxidative lignin decomposition using multifunctional LMCOs and DyPs, producing smaller metabolites such as vanillic acid, which is further degraded via ortho- and meta-ring cleavage pathways. This study not only helps to better understand the role of bacteria in lignin decomposition and thus in terrestrial ecosystems, but also expands the biocatalytic toolbox with new bacterial cells and their degradative enzymes for lignin valorization.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.20 no.3
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• pp.872-881
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• 1996

The concept of homology design has been devised for the application to large telescope structure by S.v.Hoerner. It is defined that the deformation of a structure shall be called homologous, if a given geometrical relation holds, for a given number of structural points, before, during, and after the deformation. Recently, the need of homology design in the structural design has been increase due to the required precision in the structure. Some researchers have utilized the theory on the structural design with finite element method in the late 1980s In the present investigation, a simple method using geometrical equality constraints is suggested to gain homologous deformation. The previous method is improved in that the decomposition of FEM eqation, which is very expensive, is not necessary. The basic formulations of the homology design with the optimization concept are described and several practical examples are solved to verify the usefulness and validity. Especially, a back-up structure of a satellite antenna is designed by the suggested method. The results are compared with those of existing researches.

• Journal of Microbiology and Biotechnology
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• v.23 no.12
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• pp.1699-1707
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• 2013

During the fermentative production of 1,3-propanediol under high substrate concentrations, accumulation of intracellular 3-hydroxypropionaldehyde will cause premature cessation of cell growth and glycerol consumption. Discovery of oxidoreductases that can convert 3-hydroxypropionaldehyde to 1,3-propanediol using NADPH as cofactor could serve as a solution to this problem. In this paper, the yqhD gene from Klebsiella pneumoniae DSM2026, which was found encoding an aldehyde reductase (KpAR), was cloned and characterized. KpAR showed broad substrate specificity under physiological direction, whereas no catalytic activity was detected in the oxidation direction, and both NADPH and NADH can be utilized as cofactors. The cofactor binding mechanism was then investigated employing homology modeling and molecular dynamics simulations. Hydrogen-bond analysis showed that the hydrogen-bond interactions between KpAR and NADPH are much stronger than that for NADH. Free-energy decomposition dedicated that residues Gly37 to Val41 contribute most to the cofactor preference through polar interactions. In conclusion, this work provides a novel aldehyde reductase that has potential applications in the development of novel genetically engineered strains in the 1,3-propanediol industry, and gives a better understanding of the mechanisms involved in cofactor binding.

• Journal of Life Science
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• v.15 no.5 s.72
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• pp.743-748
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• 2005

Histamine can be produced at early spoilage stage through decarboxylation of histidine in red-flesh fish by Proteus morganii, Hafnia alvei or Klebsiella pneumoniae. Allergic food poisoning is resulted from the histamine produced when the freshness of Mackerel degrades. Conversely it has been reported that there are bacteria which decompose histamine at the later stage. We isolated histamine decomposers from salted mackerel and studied the characteristics to help establish hygienic measure to prevent outbreak of salted mackerel food poisoning. All the samples were purchased through local supermarket. Histamine decomposers were isolated using restriction medium using histamine 10 species were selected. Identification of these isolates were carried out by the comparison of 16S rDNA partial sequence; as a result, we identified Pseudomonas putida strain RA2 and Halomonas marina, Uncultured Arctic sea ice bacterium clone ARKXV1/2-136, Halomonas venusta, Psychrobacter sp. HS5323, Pseudomonas putida KT2440, Rhodococcus erythropolis, Klebsiella terrigena (Raoultella terrigena), Alteromonadaceae bacterium T1, Shewanella massilia with homology of $100\%,{\;}100\%,{\;}99\%,{\;}99\%,{\;}99\%,{\;}99\%,{\;}100\%,{\;}95\%,{\;}99\%,{\;}and{\;}100\%$respectively. Turbidometry determination method and enzymic method were employed to determine the ability of histamine decomposition. Among those species Shewanella massilia showed the highest in ability of histamine decomposition. From these results we confirmed various histamine decomposer were present in salted mackerel product in the market.