• Title/Summary/Keyword: home cell

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Estrogenic Activities of Pyrethroid Compounds in MCF-7 BUS cells

  • Han, Soon-Young;Shin, Hae-Ho;Kang, Il-Hyun;Kim, In-Young;Kim, Hyung-Sik;Lee, Su-Jung;Moon, Hyun-Ju;Kim, Tae-Sung;Moon, A-Ree;Choi, Kwang-Sik
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.293.1-293.1
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    • 2002
  • Pyrethroids are extensively used as insecticide in agriculture and home. Several studies have reported that yrethroids are relatively safe to humans and wildlife. However. some studies have suggested that pyrethroids ossess estrogen-like activity. Thus. the purpose of this study was to investigate the effects of pyrethroid ompounds on cell proliferation. and expression of ERs and pS2 using estrogen receptor positive human breast ancer cell line (MCF-7 BUS celis). (omitted)

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Lipoprotein Lipase-Mediated Uptake of Glycated LDL

  • Koo, Bon-Sun;Lee, Duk-Soo;Yang, Jeong-Yeh;Kang, Mi-Kyung;Sohn, Hee-Sook;Park, Jin-Woo
    • BMB Reports
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    • v.33 no.2
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    • pp.148-154
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    • 2000
  • The glycation process plays an important role in accelerated atherosclerosis in diabetes, and the uptake of atherogenic lipoproteins by macrophage in the intima of the vessel wall leads to foam cell formation, an early sign of atherosclerosis. Besides the lipolytic action on the plasma triglyceride component, lipoprotein lipase (LPL) has been reported to enhance the cholesterol uptake by arterial wall cells. In this study, some properties of LPL-mediated low-density lipoprotein (LDL) uptake and the effect of LDL glycation were investigated in RAW 264.7 cell, a murine macrophage cell line. In the presence of LPL, $^{125}I$-LDL binding to RAW 264.7 cells was increased in a dose-dependent manner. At concentrations greater than $20\;{\mu}g/ml$ of LPL, LPL-mediated LDL binding was increased about 17-fold, achieving saturation. Without LPL, both very low-density lipoprotein (VLDL) and high-density lipoprotein (HDL) were ineffective in blocking the binding of $^{125}I$-LDL to Cells. However, LPL-enhanced LDL binding was inhibited about 50% by the presence of VLDL, while no significant effect was observed with HDL. Heat inactivation of LPL caused a 30% decrease of LDL binding. In the presence of LPL, the cells took up 40% of cell-bound native LDL. No significant difference was observed in cell binding between native and glycated LDL. However, the uptake of glycated LDL was significantly greater than that of native LDL, reaching to 70% of the total cell bound glycated LDL. These results indicate that LPL can cause the significant enhancement of LDL uptake by RAW 264.7 cells and the enhanced uptake of glycated LDL in the presence of LPL might play an important role in the accelerated atherogenesis in diabetic patients.

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Development of a disaster information delivery application using Cell Broadcast Service (긴급재난문자 서비스를 활용한 재난정보 전달 애플리케이션 개발)

  • Kim, Myeong-Jin;Kim, Kyeong-Rae;Baek, Seung-Hyeok;Oh, Seung-Hee;Jung, Woo-Sug;Kim, Kyung-Seok
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.21 no.6
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    • pp.57-63
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    • 2021
  • The Cell Broadcast Service is a public service that delivers information on disaster situations to mobile phones. In Korea, where mobile phone penetration is high, disaster information delivery through Cell Broadcast Service has a higher preference than other information delivery methods. However, since it consists only of text messages, disaster information is not delivered normally to the information vulnerable class compared to the general public. To compensate for this, a disaster information delivery application using Cell Broadcast Service was proposed and research was conducted. If research like this paper continues and Cell Broadcast Service become a service that transmits various disaster-related information considering the information vulnerable at home and abroad, it will provide the effect of reducing damage to disasters through rapid and accurate response.

Purification of Anti-ox Red Blood Cell IgG Antibody for T subset Assay (T subset정량(定量)을 위한 항우적혈구(抗牛赤血球) IgG항체(抗體)의 분리.정제(分離.精製)(I))

  • Ha, Youn-Mun;Lee, Jean-Yong;Lim, Soo-Duk
    • The Journal of the Korean Society for Microbiology
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    • v.15 no.1
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    • pp.71-75
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    • 1980
  • Antisera to ox red blood cell were prepared by intraperitoneal hypermultiple injections without adjuvant in outbred white rabits. Purified IgG fraction from these rabbits anti-ox red blood cell antiserum for T subset assay was obtained by precipitation with 50% saturated ammonium sulphate followed by DEAE-cellulose chromatography and Sephadex G-150 gel filtration. These purified IgG fraction was compared with Cappel company standard IgG fraction for $T_G$ subpopulation assay. We used home-made IgG fraction and obtained favorable results in $T_G$ subopulation assay as Cappel company standard IgG fraction.

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Dynamic Reservation Scheme of Physical Cell Identity for 3GPP LTE Femtocell Systems

  • Lee, Poong-Up;Jeong, Jang-Keun;Saxena, Navrati;Shin, Ji-Tae
    • Journal of Information Processing Systems
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    • v.5 no.4
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    • pp.207-220
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    • 2009
  • A large number of phone calls and data services will take place in indoor environments. In Long Term Evolution (LTE), femtocell, as a home base station for indoor coverage extension and wideband data service, has recently gained significant interests from operators and consumers. Since femtocell is frequently turned on and off by a personal owner, not by a network operator, one of the key issues is that femtocell should be identified autonomously without system information to support handover from macrocell to femtocell. In this paper, we propose a dynamic reservation scheme of Physical Cell Identities (PCI) for 3GPP LTE femtocell systems. There are several reserving types, and each type reserves a different number of PCIs for femtocell. The transition among the types depends on the deployed number of femtocells, or the number of PCI confusion events. Accordingly, flexible use of PCIs can decrease PCI confusion. This reduces searching time for femtocell, and it is helpful for the quick handover from macrocell to femtocell. Simulation results show that our proposed scheme reduces average delay for identifying detected cells, and increases network capacity within equal delay constraints.

Inhibitory Effects of Cyrtopodion scabrum Extract on Growth of Human Breast and Colorectal Cancer Cells

  • Amiri, Ahmad;Namavari, Mehdi;Rashidi, Mojtaba;Fahmidehkar, Mohammad Ali;Seghatoleslam, Atefeh
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.2
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    • pp.565-570
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    • 2015
  • Breast and colorectal cancers rank high in Iran as causes of mortality. Most of the current treatments are expensive and non-specific. The potential anticancer properties of common home gecko, Cyrtopodion scabrum, were investigated in this study. The effects of C. scabrum extract on proliferation, viability and migration of the colorectal cancer (SW-742), breast cancer (MCF-7) and normal (MSC) cell lines were investigated using MTT and in vitro wound healing assay. $IC_{50}$ values calculated for the extract were $559{\pm}28.9{\mu}g/mL$ for MCF-7 and $339{\pm}11.3{\mu}g/mL$ for SW-742. No toxic effects on the normal control cells were observed. MCF-7 and SW-742 cell growth was inhibited by 32.6% and 62%, under optimum conditions, compared to the untreated control cells. The extract also decreased the motility and migration ability of both cancer cell lines, with no significant effects on the normal control cells. Data suggest C. scabrum extract as a useful natural resource for targeting cancer cells specifically.

Menadione-induced Cytotoxicity in Rat Platelets: Absence of the Detoxifying Enzyme, Quinone Reductase

  • Kim, Kyung-Ah;Kim, Mee-Jeong;Ryu, Chung-Kyu;Chang, Moon-Jeong;Chung, Jin-Ho
    • Archives of Pharmacal Research
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    • v.18 no.4
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    • pp.256-261
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    • 1995
  • The elevation of intracellular $Ca^{2+}$ in various tissue through oxidative stress induced by menadione has been well documented. Increase of $Ca^{2+}$ level inplatelets results in aggreaction of patelets. To test the hypothesis that menadione-induced $Ca^{2+}$ elevations can play a role in platelet aggregation, we have studied the effect of menadione on aggragation of platelets isolated from female rats. Treatment with menadione to platelet rich plasma (PRP), which proved to be 60% as determined by aggregometry. however, exposure of PRP to menadione leads to a loss of cell viability, as measured by lactae dehydrogenase (LDH) leakage, suggesting that menadione might induce cell lysis rather than aggregation of platelets. Turbidty changes induced by menadione were unaffected by addition ofl dicoumarol, which is a quinone reducellular factions of patelets. These data, which indicate an absence of the QR detoxifying pathway, suggest that platelets may be more susceptible to menadione-induced cytotoxicity than certain other cell, as hepatocytes.

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Manufacturing of Ag Nano-particle Ink-jet Printer and the Application into Metal Interconnection Process of Si Solar Cells (Si 태양전지 금속배선 공정을 위한 나노 Ag 잉크젯 프린터 제작 및 응용)

  • Lee, Jung-Tack;Choi, Jae-Ho;Kim, Ki-Wan;Shin, Myoung-Sun;Kim, Keun-Joo
    • Journal of the Semiconductor & Display Technology
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    • v.10 no.2
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    • pp.73-81
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    • 2011
  • We manufactured the inkjet printing system for the application into the nano Ag finger line interconnection process in Si solar cells. The home-made inkjet printer consists of motion part for XY motion stage with optical table, head part, power and control part in the rack box with pump, and ink supply part for the connection of pump-tube-sub ink tanknozzle. The ink jet printing system has been used to conduct the interconnection process of finger lines on Si solar cell. The nano ink includes the 50 nm-diameter. Ag nano particles and the viscosity is 14.4 cP at $22^{\circ}C$. After processing of inkjet printing on the finger lines of Si solar cell, the nano particles were measured by scanning electron microscope. After the heat treatment at $850^{\circ}C$, the finger lines showed the smooth surface morphology without micropores.

Effects of Panicum miliaceum L. extract on adipogenic transcription factors and fatty acid accumulation in 3T3-L1 adipocytes

  • Park, Mi-Young;Seo, Dong-Won;Lee, Jin-Young;Sung, Mi-Kyung;Lee, Young-Min;Jang, Hwan-Hee;Choi, Hae-Yeon;Kim, Jae-Hyn;Park, Dong-Sik
    • Nutrition Research and Practice
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    • v.5 no.3
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    • pp.192-197
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    • 2011
  • The dietary intake of whole grains is known to reduce the incidence of chronic diseases such as obesity, diabetes, cardiovascular disease, and cancer. To investigate whether there are anti-adipogenic activities in various Korean cereals, we assessed water extracts of nine cereals. The results showed that treatment of 3T3-L1 adipocytes with Sorghum bicolor L. Moench, Setaria italica Beauvois, or Panicum miliaceum L. extract significantly inhibited adipocyte differentiation, as determined by measuring oil red-O staining, triglyceride accumulation, and glycerol 3-phosphate dehydrogenase activity. Among the nine cereals, P. miliaceum L. showed the highest anti-adipogenic activity. The effects of P. miliaceum L. on mRNA expression of peroxisome proliferator-activated receptor-${\gamma}$, sterol regulatory element-binding protein 1, and the CCAAT/enhancer binding protein-${\alpha}$ were evaluated revealing that the extract significantly decreased the expression of these genes in a dose-dependent manner. Moreover, P. miliaceum L. extract changed the ratio of monounsaturated fatty acids to saturated fatty acids in adipocytes, which is related to biological activity and cell characteristics. These results suggest that some cereals efficiently suppress adipogenesis in 3T3-L1 adipocytes. In particular, the effect of P. miliaceum L. on adipocyte differentiation is associated with the downregulation of adipogenic genes and fatty acid accumulation in adipocytes.

Production of Plant Protein Concentrate and Yeast Biomass from Radish Greens (무청즙액을 이용한 녹엽단백질과 효모균체의 생산)

  • Rhee, Yeong-Sang;Kyung, Kyu-Hang;Yoo, Yang-Ja
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.3
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    • pp.263-269
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    • 1992
  • Radish green juice was used as a dual source for the production of plant protein precipitate and Candida utilis biomass. Precipitates ranging from 10.0 to 16.5g were obtained from a liter of radish green juice by heating at 80-10$0^{\circ}C$C for 1 to 10 min or by modification of the pH of radish green juice. Crude protein content of the precipitate was between 25 and 38%. The residue remaining after protein precipitation was used in turn for the cultivation of the yeast, C. utilis, in order to produce yeast biomass. C. utilis grew well in radish green residual juice and completed growth within 24 hr at 3$0^{\circ}C$ and 200rpm in shake flask experiments. Maximum dry cell weight obtainable from a liter of radish green residual juice was 19.5g, when the yeast was grown on the juice residue diluted 3 times or more with water to make sugar content be equal to or less than about 1.0%. Supplementation of 3-fold diluted radish green residual juice with yeast extract and (NH$_4$)SO$_4$ enhanced yeast biomass production and cell protein content significantly. Total high protein material obtainable from a liter of radish green juice was 33.0g.

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