• Title/Summary/Keyword: holding solution

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Antimicrobial Effect of Free Available Chlorine on Postharvest Life of Cut Rose 'Brut' (절화 장미 'Brut'의 절화수명에 미치는 유리염소의 항균효과)

  • Lee, Young Boon;Kim, Wan Soon
    • Horticultural Science & Technology
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    • v.31 no.3
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    • pp.317-321
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    • 2013
  • This study was conducted to investigate the antimicrobial effect of freely available chlorine (FAC) on the vase life of cut rose 'Brut' (Rosa hybrida L.). Postharvest treatments to extend the vase life of cut roses were divided into holding solution treatment and pulsing solution treatment. In holding solution treatment, the cut roses were treated with the preservative solutions containing FAC (0, 10, 20, and $40mg{\cdot}L^{-1}$) and sucrose (0 and 2%, w/v). In pulsing solution treatment, cut roses were dipped into the FAC solutions of 100, 200, and $400mg{\cdot}L^{-1}$ for 10 seconds. The longest vase life of cut roses was observed in the holding solution with FAC $20mg{\cdot}L^{-1}$ as 12 days, followed by pulsing with $400mg{\cdot}L^{-1}$ as 11 days, which were four or five days longer than the control. In addition, relative fresh weight and water uptake were the highest in the holding solutions with FAC 20 and $40mg{\cdot}L^{-1}$. The antimicrobial effect of FAC in vase solution was lasted for eight days after treatment, which was offset by sucrose addition. FAC contents in the FAC holding solution mixed with sucrose were exhausted by 88% two days after treatment, whereas only 15% of FAC was reduced in the holding solution without sucrose. This study indicated that FAC can be applied to extension of the postharvest life of cut roses by antimicrobial activity.

Effects of Earthenware Vase on Vase Life of Cut Gerbera Flowers and Microbial Multiplication (옹기 화병이 절화 거베라의 수명 및 미생물 증식에 미치는 영향)

  • Lim, Young-Hee;Oh, Wook
    • Horticultural Science & Technology
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    • v.29 no.2
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    • pp.110-115
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    • 2011
  • To examine the availability of onggi (earthenware) as a floral vase, we compared the vase life of cut gerberas (Gerbera jamesonii) with generally used vases made of glass and porcelain. After holding cut gerbera 'Honeymoon' and 'Golden Time' in vases made of glass, porcelain, and onggi containing holding solution (tap water or preservative solution), vase life, fresh weight, and water uptake of cut flowers, and total number of microbes in holding solution were collected. In onggi vase, vase life of both the cultivars was longer than the other vases and floral preservative prolonged vase life compared with tap water. Cut gerberas applied with preservative solution in onggi vase maintained their ornamental value for more than 14 days. Cumulative water uptake by cut gerberas was greatest in onggi vase, where 'Honeymoon' and 'Golden Time' absorbed water by 19.2 and 15.8 mL, respectively, for 10 days. Fresh weight of cut flowers in onggi vase was greater than those of the others, and the preservative solution delayed the reduction of fresh weight compared with tap water. Especially, onggi vase with preservative solution maintained 89% of the initial fresh weight at the 8th day of the treatments due to smooth water uptake with comparison with 71% in porcelain. Total number of microbes in the holding solution was smallest in onggi vase. At the 8th day, microbes number in onggi vase was 435 cfu/mL, that was 20-30% level of those of glass and porcelain vases. In conclusion, onggi vase inhibited microbial proliferation in holding solution, maintained water uptake by cut flower, and delayed bending of flower stalks due to vascular blockage, and then prolonged vase life in gerbera. Therefore, onggi with numerous micropores will be a good material for flower vase and preservative solution can improve its function.

An Optimization Method for Self-Boring Pressuremeter Holding Test to Determine a Horizontal Coefficient of Consolidation under Partial Drained Soil Conditio (부분배수가 발생하는 지반의 수평압밀계수 결정을 위한 자가굴착식 프레셔메터 유지시험의 최적화 해석법)

  • Kim, Young-Sang
    • Proceedings of the Korean Geotechical Society Conference
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    • 2005.03a
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    • pp.370-375
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    • 2005
  • This paper describes a systematic way of identifying the horizontal coefficient of consolidation for clayey soil under undrained condition and silty soil under partial drained condition by applying an optimization technique to the early part of dissipation data measured from the self-boring pressuremeter strain holding test. An analytical solution developed by Randolph & Wroth (1979) was implemented in normalized form to express the build-up and dissipation of excess pore pressures around a pressuremeter as a function of the rigidity index. Horizontal coefficient of consolidation was determined by minimizing the differences between theoretical and measured excess pore pressure curves using optimization technique. It was found that the proposed optimization technique can evaluate in-situ horizontal coefficient of consolidation rationally, which is similar with that obtained from the piezocone dissipation test. Furthermore, proposed method can evaluate appropriate coefficient of consolidation for soil under partially drained condition.

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Effect of Chitosan and Sucrose on the Vase Life of Cut Rose 'Cardinal' (장미 'Cardinal'의 절화수명에 미치는 키토산과 Sucrose의 영향)

  • Yoo, Yong Kweon;Park, Hyun Jin;Kang, Sang Wook;Kim, Hyun Kyung
    • Horticultural Science & Technology
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    • v.17 no.4
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    • pp.481-484
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    • 1999
  • This experiment was conducted to examine the effects of chitosan and sucrose on the vase life of cut rose (Rosa hybrida L. cv. Cardinal). Addition of 5 mg/L chitosan and 3% sucrose to the holding solution prolonged the vase life for 3 days as compared to the control, and improved quality of cut rose in flower diameter and fresh weight. Development of bent-neck was delayed by supply of 2 mg/L or 5 mg/L chitosan and 3% sucrose about 2 or 3 days. When 3% sucrose was added to the holding solution, the rate of water absorption more slowly decreased compared to the other treatments.

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Dissolution Behavior of Complex Carbonitrides in a Microalloyed Steel (Microalloyed 강에서 복합 탄질화물의 재용해 거동)

  • Jung, Jae-Gil;Park, June-Soo;Ha, Yang-Soo;Lee, Young-Kook;Bae, Jin-Ho;Kim, Kisoo
    • Journal of the Korean Society for Heat Treatment
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    • v.21 no.6
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    • pp.287-292
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    • 2008
  • Dissolution behavior of complex carbonitrides in a Nb-Ti-V microalloyed steel was quantitatively examined by electrical resistivity, transmission electron microscopy (TEM), and optical microscopy. The electrical resistivity increased with solution treatment temperature up to $1250^{\circ}C$ for a holding time of 15 min. But, an increasing rate of electrical resistivity with temperature was obviously decreased above $1150^{\circ}C$. As the solution treatment temperature increases, irregular shaped Nb-rich carbonitrides disappear and cuboidal Ti-rich carbonitrides are observed. Abnormal grain growth occurs above $1250^{\circ}C$ for a holding time of 15 min. The optimal solution treatment temperature of a Nb-Ti-V microalloyed steel was determined as $1200^{\circ}C$ for a holding time of 15 min.

Antimicrobial Effect of Chlorine Dioxide on Vase Life of Cut Rose 'Beast' (Chlorine Dioxide가 절화장미 수명연장에 미치는 항균효과)

  • Lee, Young Boon;Kim, Wan Soon
    • Horticultural Science & Technology
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    • v.32 no.1
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    • pp.60-65
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    • 2014
  • This study was conducted to investigate the antimicrobial effect of chlorine dioxide ($ClO_2$) on the vase life of cut rose 'Beast' (Rosa hybrida L.). Postharvest treatments to extend the vase life of cut roses were divided into two: holding solution treatment and pulsing solution treatment. In holding solution treatment, the cut roses were treated with preservative solutions containing tap water (TW, control), distilled water (DW), $ClO_2$ 2, 4, 6, and $8{\mu}L{\cdot}L^{-1}$, and compared with a commercialized antimicrobial compound of 8-HQS $200{\mu}L{\cdot}L^{-1}$. In pulsing solution treatment, cut roses were dipped into the $ClO_2$ solutions of 50, 100, 150, 200, and $250{\mu}L{\cdot}L^{-1}$ for 60 seconds and were placed in DW. The air temperature was $18.4^{\circ}C$, RH 51.5%, and light (photosynthetically active radiation, PAR) $3.6{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ with 12 hour day length. The longest vase life of cut roses was observed in the holding solution with $ClO_2$ $4{\mu}L{\cdot}L^{-1}$ as 13.8 days and pulsing with $200-250{\mu}L{\cdot}L^{-1}$ as 13.5-13.7 days, where vase life were extended four days longer than TW. Whereas, the inclusion of 8-HQS $200{\mu}L{\cdot}L^{-1}$ in vase solution resulted in phytotoxicity. The relative fresh weight and water uptake have similar tendencies. Bacteria inhibition by $ClO_2$ and 8-HQS were very effective. But bacteria at TW and DW treatments on cut flower with stem were detected in $3.7{\times}10^5CFU{\cdot}L^{-1}$ and $6.3{\times}10^5CFU{\cdot}L^{-1}$, respectively (without stem in DW $1.4{\times}10^4CFU{\cdot}L^{-1}$). The $ClO_2$ contents in holding solution of all treatments were scavenged in two-four days after treatment. This study indicated that $ClO_2$ $4{\mu}L{\cdot}L^{-1}$ holding solution treatment and $200-250{\mu}L{\cdot}L^{-1}$ pulsing solution treatment can be applied to extend the postharvest life of cut roses.

Effect of Cycloheximide and Holding Solution on Vase Life of Cut 'Blue Magic' Iris Flowers according to the Flower Development and Opening Stages (Cycloheximide 및 보존용액 처리가 개화단계에 따른 절화 아이리스 '블루매직'의 수명에 미치는 영향)

  • Kim, Young-A;Lee, Jong-Suk
    • Horticultural Science & Technology
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    • v.28 no.5
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    • pp.790-795
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    • 2010
  • This experiment was conducted to clarify the effects of cycloheximide and holding solution on vase life of cut 'Blue Magic' iris. The vase life of iris flowers held in 3% sucrose (S) + $100mg{\cdot}L^{-1}$ hydroxy quinoline sulfate (HQS) + $50mg{\cdot}L^{-1}$ $AgNO_3$ + $100mg{\cdot}L^{-1}$ Benzylaminopurine (BA), 3% S + $100mg{\cdot}L^{-1}$ HQS + $10{\mu}M$ cycloheximide (CHI), or 3% S + $100mg{\cdot}L^{-1}$ HQS + $50{\mu}M$ CHI were much longer than those held in distilled water. Squeeze stem phenomenon that showed at a holding solution containing $200mg{\cdot}L^{-1}$ HQS disappeared at a holding solution containing $100mg{\cdot}L^{-1}$ HQS. The holding solution containing 3% S + $100mg{\cdot}L^{-1}$ HQS + $50mg{\cdot}L^{-1}$ $AgNO_3$ + $100mg{\cdot}L^{-1}$ BA extended the most effective treatments on vase life, fresh weight, water balance, and flowering of cut iris flowers. However, the holding solution containing 3% S + $100mg{\cdot}L^{-1}$ HQS + $10{\mu}M$ CHI and 3% S + $100mg{\cdot}L^{-1}$ HQS + $50{\mu}M$ CHI was not effective in solution uptake or transpiration, but did result in high water balance. Iris flowers treated with CHI at the half-open flower stage showed increases in ornamental value, such as full flower opening and extended vase life. To improve flower quality and prolonging vase life of cut iris flowers, a holding solution containing $50{\mu}M$ CHI can be used continuously from the half-open stage.

Is Higher Land Holding Tax the Solution for Korea's Land Problems? (토지보유과세강화(土地保有課稅强化)의 당위성(當爲性)에 대한 검토(檢討))

  • Son, Jae-young
    • KDI Journal of Economic Policy
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    • v.14 no.3
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    • pp.49-72
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    • 1992
  • This paper examines the increasingly popular belief that higher holding tax will be the ultimate solution for Korea's land problems which include excessive concentration of ownership, high and rapidly increasing land prices, and rampant speculation. In principle, land holding tax can supplement capital gains tax in recapturing capital gains from land or suppress returns from land investment returns in line with other forms of asset. This paper shows, however, that the tax burden must be drastically increased for the tax to achieve such goals, and the resistance from tax payers is sure to be intense. As long as the price expectation remains high, as in Korea where land prices have increased 19% annually during the past 18 years, even such increase in the tax may have little impact on landlords' behaviors, the price trend, or the ownership structure. More effective solutions for Korea's land problems are relaxing land use regulations to encourage the supply for urban land and improving the performance of capital gains tax to recapture windfall gains from land. This paper also notes that the so-called "lock-in effect" of the capital gains tax seems to be exaggerated. Land holding tax should be viewed as a revenue raiser for local governments rather than an anti-speculative policy tool. Abandoning unattainable policy goals and adhering to the general principles of taxation, will make land holding tax much simpler, and will better function as a local revenue source.

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Determination of Horizontal Coefficient of Consolidation from the Self-boring Pressuremeter Holding Test by Considering Pore Pressure Dissipation Trend (간극수압 소산경향을 고려한 자가굴착식 프레셔메터로부터의 수평압밀계수 결정법)

  • 김영상
    • Journal of the Korean Geotechnical Society
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    • v.20 no.3
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    • pp.151-159
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    • 2004
  • This paper describes a systematic way of identifying the horizontal coefficient of consolidation of clayey soil by applying an optimization technique to the early part of dissipation data measured from the self-boring pressuremeter strain holding test. An analytical solution developed by Randolph & Wroth (1979) was implemented in normalized form to express the build-up of excess pore pressures as a function of the rigidity index and subsequent dissipation of excess pore pressures around a pressuremeter Horizontal coefficient of consolidation was determined by minimizing the differences between theoretical and measured excess pore pressure curves over 50% degree of dissipation range using optimization technique. The effectiveness of the proposed back-analysis method was examined against the real fled performances obtained from pressuremeter strain holding tests at Gimje and Yangsan site. It is shown that the proposed back-analysis method can evaluates the rational horizontal coefficient of consolidation, which is similar to those obtained from the piezocone dissipation test. Furthermore, proposed method can evaluate appropriate coefficient of consolidation for soil under partially drained condition.

Establishment of Freezing Conditions for Improving Cryosurvival in Miniature Pig Spermatozoa (미니돼지 정액의 동결-융해 후 생존율 향상을 위한 동결 조건 확립)

  • Lee, Yong-Seung;Yoo, Han-Jun;Cheong, Hee-Tae;Yang, Boo-Keun;Woo, Jea-Seok;Park, Choon-Keun
    • Reproductive and Developmental Biology
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    • v.35 no.3
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    • pp.329-334
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    • 2011
  • This study was conducted to establish a freezing method of miniature pig spermatozoa. The semen 더aculated from PWG M-type miniature pig was collected by gloved-hand method. The semen was diluted with same volume extender (m-Modena B). The frozen solution used frozen solution of four different (LEY, TCG, BF-5 and m-Modena+egg yolk) for find optimal frozen solution in miniature pig sperm. The diluted semen for frozen rate assay was added to LEY solution (solution I: 11% lactose+egg yolk; solution II: solution I+glycerol+OEP), and frozen depending on freezing rate by the three different freezing methods (A: until $5^{\circ}C$ for 1 hrs, holding at $-102^{\circ}C$ for 10 min; B: until $5^{\circ}C$ for 2 hrs, holding at $-102^{\circ}C$ for 10 min; C: until $5^{\circ}C$ for 3 hrs, holding at -80 and $-102^{\circ}C$ for 10 min). Semen cooled until $5^{\circ}C$ was added with glycerol 1, 3 and 5%, and take a equilibrium time for 0, 10 and 30min. Frozen-thawed sperm were evaluated for viability, acrosomal status and morphological abnormality. The results of frozen-thawed sperm ability by frozen solution, viability was higher in LEY solution compared to other three different frozen solution. AR pattern of LEY solution were lower than other three different frozen solution. The results of freezing rate, viability was higher in B method compared to other methods (p<0.05). Acrosomal statute was intacted in A and B methods than C method. The experiment for glycerol condition was showed that sperm viability was higher in extender with 1% and 3% glycerol and equilibrium time of 0 min. The acrosome damage was lower in extender with 1% glycerol and equilibrium time of 10 min than other conditions. In conclusion, the optimal conditions for cryopreservation of miniature pig spermatozoa obtained in LEY frozen solution, cooling rate of 1~2 hrs, 1~3% glycerol concentrations and glycerol equilibrium time of 0~10 min.