• 대한치과교정학회지
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• 제36권3호
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• pp.171-177
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• 2006

마이크로 임플랜트는 순티타늄과 티타늄 합금을 사용하고 있다. 순티타늄의 경우 grade가 낮을 수록 생체적합성은 증가하나 기계적 성질이 낮다. 이에 본 연구에서는 순티타늄 grade 2와 grade 4를 재료로 한 마이크로 임플랜트를 각각 토끼의 다리뼈에 식립한 후 2, 8, 12주의 시간경과에 따른 조직학적 소견의 차이와 식립, 제거 시의 최대토크의 측정으로 그 티타늄 종류에 따른 차이를 살펴보았다. 토끼의 경골에 식립한 티타늄의 종류에 따른 식립시 및 시간의 경과에 따른 제거시의 토크를 측정하여 다음과 같은 결론을 얻었다. 첫째, grade 2와 grade 4간의 식립 시 최대 토크의 차이는 없었다. 둘째, 식립 후 2, 8, 12주 후의 제거 시 최대토크 측정에서 전체 마이크로 임플랜트의 그룹간 차이는 없었다. 셋째, 2주에서 8주로 시간이 경과함에 따라 제거 시 최대토크가 유의하게 증가하였으며 8주와 12주간에는 유의한 상관관계가 없었다. 따라서 유지력은 grade 2 티타늄과 비슷하지만 물리적 성질이 더 단단한 grade 4 티타늄을 마이크로 임플랜트 재료로 사용하는 것이 좋은 것으로 생각된다.

• The Korean Journal of Pain
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• 제7권2호
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• pp.162-169
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• 1994

Blockade of cervicothoracic sympathetic ganglion (stellate ganglion controls pain on face, head, neck, shoulder, upper limbs, and upper chest, including their viscera and sympathetically maintained pain. This procedure also increases blood flow to the above areas and relieves hyperreactivity of sympathetic nervous system. Clinically, repeated stellate ganglion blocks with local anesthetic agent may become difficult with complications such as accidental intravascular or subdural injection, recurrent laryngeal nerve or bracheal plexus paralysis, pneumothorax and edema on injection site. Therefore, at times long-term cervicothoracic ganglion block with neurolytics is necessitated but its applications are prohibited by the critical structures surrounding ganglion. There are also few reports of neurolytic stellate ganglion block. This study was performed to observe the complications, gross changes of surrounding structures, and microscopic findings of ganglion cells after neurolytic block and to certify the possibility of clinical use of neruolytic stellate ganglion block. The unilateral superior cervical sympathetic ganglion of rabbit was blocked with absolute ethyl alcohol 0.4 ml at the level of cricoid cartilage. Normal ganglion was used as a control and 5 animals were sacrificed at each intervals of 7, 15 and 50 days after block. The results were as follows; 1) All experimental animals showed no specific changes of behavior, motor function. No necrotic tissues were present in the block area during the observation period. There were some gross scar tissues along the fascia of muscles surrounding the needle injection site, but gross atrophy of muscles or injured major vessels were not found. 2) Microscopically, structures of normal ganglion of rabbit were very similar to those of humans. Seven days after absolute ethyl achohol injection there were marked edema of ganglion cells and nuclei with irregular nuclear membrane. Some of the ganglion cells lost their nuclei and showed degenerative changes. Fifteen days after block, cell edema were decreased and loss of the Nissl's body was prominant. The ganglion cell structures looked close to normal but the cytoplasm and nucleus were generally contracted 50 days after block. These results suggest absolute ethyl alcohol injection on cervical sympathetic ganglion with above method mainly blocks pre- and post-synaptic fibers and the long-term neurolytic blockade of this ganglion may be possible in rabbits.

• 한국패류학회지
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• 제19권2호
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• pp.117-124
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• 2003

동해 중부 해안 하천에 서식하는 기수산 재첩의 자원관리와 인공종묘 생산을 위한 생식생물학적 조사를 위해 2000년 11월부터 2001년 10월까지 강원도 양양군 소재 남대천 하구에서 매월 30-40 개체씩 채집하여 성장, 생식소지수, 배우자 형성과정 및 생식주기, 성비 등을 조사하였다. 강원도 일본재첩은 자웅이체 난생종으로 암컷의 생식소는 회흑색, 수컷의 생식소는 유백색을 띄어 육안적으로 86% 이상의 구별가능성을 보였다. 성숙한 난모세포는 약 80${\mu}m$직경을 보였다 생식주기는 초기활성기 (4-6월), 후기활성기 (5-6월), 완숙기 (6-9월), 부분산란기 (6-9 월), 퇴화 (9-1월) 및 비활성기 (2-4월)의 연속적인 5 단계로 구분할 수 있었다. 비교적 긴 산란기를 보였으며, 방란,방정 후 생식소 자체가 완전히 퇴화되지 않고 새로운 조직에서 신생되면서 남부지방산보다 늦은 이듬해 4월까지 긴 비활성기를 거치고 4월 중순부터 다시 분화가 활발히 개시되었다 . 생식소지수는 생식주기의 변동과 일치하였으며 연중 365 개체 중 자웅동체 현상은 1 개체에서 관찰되었다. 95% 신뢰한계 내에서 암컷 대 수컷의 성비는 1:1로 유의한 차이를 보이지 않았다 (x$^2$ = 1.22, p > 0.05).

• 한국임상수의학회지
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• 제27권3호
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• pp.289-294
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• 2010

경남 산청지방에서 쏘가리를 양식하던 어장에서 대량폐사가 발생하였다. 주요 증상으로 전신 피부에서 궤양이 진행되었고 조직학적으로는 주요 실질 장기에서 세균의 집락과 주변부위로 염증 및 괴사가 관찰되었다. 분리된 세균을 API 20E 및 API 20NE를 이용하여 생화학적 검사를 실시한 결과 Aeromonas salmonicida subsp. salmonicida (typical A. salmonicida)로 진단할 수 있었다. 그러나 atypical A. salmonicida와 typical A. salmonicida로 구분할 수 있는 PCR 기법을 실시한 결과, 본 세균은 atypical A. salmonicida임을 확인할 수 있었다. 본 연구는 우리나라 쏘가리에서 A. salmonicida로 인한 발병의 최초 보고임과 동시에, A. salmonicida의 정확한 진단을 위해선 생화학적 검사가 아닌 PCR 검사를 이용한 진단을 실시해야 함을 제시하였다.

• 동의생리병리학회지
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• 제22권3호
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• pp.562-568
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• 2008

Theosteoporosis, which is occurred after menopause, is a kind of metabolic bone disorder. It develops when the bone mass begins to decrease radically and its main symptoms are the bone fracture and the height-shortening. This thesis aims at what effects the Phlomis umbrosa will have for the prevention of the osteoporosis in the SD-rat that is caused by the ovariectomy. The 24 female white rats, after their ovaries are removed, were divided into the Normals, the Control group, Phlomis umbrosa Administrated group. After then, for 8 weeks, D.W to the normals and the control, and the Phlomis umbrosa(46 mg/100 g) to the Phlomis umbrosa group were given in the mouths of them. After 8 weeks the rats were sacrificed. And the weight, albumin, AST, ALT, total-cholesterol, triglyceride, P, Ca, T4, estradiol, the weight of the femur, the amount of tibia ash, the area of trabecular bone, the thickness of trabecular bone were measured. The serum analysis shows that the total cholesterol of the Phlomis umbrosa Administrated group reduced remarkably, compared to that of the control group. AST, ALT, ALP, T4 of Phlomis umbrosa group were decreased, but not in distinct measure. Estradiol of Phlomis umbrosa group increased substantially compared to that of the control group, but albumin, triglyceride, Phosphorous, calcium of the group showed the tendency to increase, but not so remarkably. The histological observation shows that the area and the thickness of (trabecular bone), the number of osteoclast of the Phlomis umbrosa groups increased significantly compared to those of the control group. Conclusions: The above experiment shows that Phlomis umbrosa has the prevention effect by supplying estradiol.

• 생약학회지
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• 제28권3호
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• pp.104-111
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• 1997

The traditional herbal medicine, Jackyakgamcho-tang(JGT), was reported to decrease serum testosterone levels and make pregnancy possibel in anovulatory woman and rat. JGT contains Paeoniae Radix(PR) and Glycyrrhizae Radix(GR) in equal amount. This study was designed to investigate the effect of JGT and its components(PR, GR, paeoniflorin and glycyrrhizin) on uterine and ovarian responses, follicular development, and estrogen secretion in the immature rat. The samples(water extracts of JGT, PR, GR; pure compound of paeoniflorin and glycyrrhizin) were administered orally to rats from the 21th day of age to the 28th or 30th days of age for 7 or 9 days. JGT(400mg/kg) and PR(100mg/kg, 200mg/kg) treatments significantly increased serum estradiol above levels in control rats, but both GR and glycyrrhizin had no effect on this parameter. Gross observation and histological analysis revealed that an increased number of growing follicules was observed in the ovaries of JGT and PR treated rat. However the lutenized follicles and ova present in the oviducts were not observed in all rats except one treated with estrogen as a positive control. These results indicate that JGT stimulates the estrogen production and follicular maturation in the immature rat and PR is the main component to induce such reaction.

• 대한후두음성언어의학회지
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• 제4권1호
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• pp.33-41
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• 1991

1983년도부터 1990년까지 7년 동안 연세대학교 의과대학 영동세브란스병원 이비인후과에 내원하여 후두각화증으로 조직학적 확진을 거친 환자를 대상으로 후향적 조사를 실시하여 다음과 같은 결과를 얻었다. 1) 총 환자수는 26명으로서 남녀비는 22:4로 남자에서 월등히 높았으며 연령별로는 남자는 50대, 여자는 50대 및 60대에서 빈도가 높은 것으로 나타났다. 2) 침윤성 암 1례를 포함해서 이형성 후두각화증을 보인 예는 총 10례였는데 역시 남자에서 높은 빈도를 보였다. 3) 조직학적으로 크게 3가지로 분류하였는데 단순성 각화증이 16례(61.5%), 국소적 이형성이 있는 각화증이 7례(26.9%)이었고 상피내암(Carcinoma in situ)은 2례, 침윤성 암(Carcinoma) 1례로 확인되었다. 4) 주 증상으로는 쉰 목소리를 전례에서 호소하였으며 증상이 나타나면서 내원하기까지의 지속시간이 24개월 이하가 19례(73.1%)로 조사되었다. 5) 흡연 경력은 17례(65.4%)이며 10-20년이 10례(38.8)로 많은 빈도수를 보였다. 6) 재발은 모두 9례로서 1례가 침윤성 암으로 재발하였다.

• 한국가금학회지
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• 제13권2호
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• pp.197-208
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• 1986

본 시험은 6가지 다른 사양방법이 White Plymouth Rock 육용종 웅계의 생체중, 정소, 계관 그리고 뇌하수체의 발육 및 성성숙에 미치는 영향을 구명하기 위하여 부화후부터 22주령까지 2주간격으로 생체중, 정소, 계관 그리고 뇌하수체의 중량이 조사되었으며, 또한 정소조직과 정액성상이 조사되었다. 그 얻어진 결과를 요약하면 다음과 같다. 1. 생체중, 정소, 계관의 발육은 10주령 이후부터 무제한급이군들이 제한급이군들(무제한급이군들의 70%급이)보다 2주 더 빠름을 나타내었다. 정소 및 계관의 급증시기는 무제한급이군들에서 20주령이었고, 제한급이군들에서 22주령이었다. 2. 뇌하수체의 무게는 부화후부터 22주령까지 4주령, 14주령, 16주령을 제외하고 각 주령별, 각 처리군간에 유의성이 나타나지 않았다. 뇌하수체의 중량은 주령이 경과하면서 완만한 증가를 나타내었다. 3. 22주령까지에서 주령, 생체중, 정소, 계관 및 뇌하수체간에는 고도의 유의적인 상관관계를 나타내었다. 4. 세정관의 관경과 강경은 부화후부터 10주령까지 점진적으로 증가되다가 무제한 급이군들에서는 12주령부터 제한급이군들에서는 14주령부터 급증하기 시작하였고, 그 후에도 22주령까지 계속증가되었다.

• 대한핵의학회지
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• 제12권2호
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• pp.33-40
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• 1978

Clinical features of 147 patients with biopsy-proven thyroid carcinomas were investigated from January, 1972 to April, 1978 at the Seoul National University Hospital with the following results. 1) The incidence of thyroid carcinomas according to their histopathological classification revealed 76.2% of papillary carcinoma, 19.0% of follicular carcinoma, and 3 cases of occult sclerozing carcinoma, 1 case of giant cell carcinoma and 1 case of metastatic melanoma. 2) The ratio of male to femle patients was 1:8.3 and showed no difference between papillary and follicular carcinomas. 3) The age distribution showed the peak incidence in the fourth decade (29.3%) followed by the fifth and sixth decades. 4) The average duration of illness from the onset of symptoms was about 5 years while it was 4.4 years and 7.6 years in the papillary and follicular carcinomas respectively. 5) The diameter of the thyroid masses was smaller than 5 cm in 53.6% of the patients, from 5 cm to 10 cm in 40.0% and larger than 10 cm in 6.4%. 6) In 36.4% of the patients with thyroid carcinomas the thyroid masses were fixed to adjacent tissues. 7) Metastasis to the regional lymph nodes was noted in 40.0% of the total cases, and in 45.2% and 17.6% of the papillary and follicular carcinomas respectively, while the lung and bone metastases were found in 10.0% and 4.4% in each type respectively. 8) 88.9% of the patients showed cold areas in the thyroid scans using $^{131}I$. 9) Typical psammoma bodies were observed in 21.3% of the cases in the microscopic examination of the pathological specimens. 10) The initial diagnosis of thyroid malignancy could be made before histological confirmation in 64.5% of the patients. 11) The clinical staging slightly modified from Schulz method revealed 43.6% of the patients in stage I, 26.4% in stage II, 20.9% in stags III and 9.1 % in stage IV. 12) The association with Hashimoto's thyroiditis was noted in 4 cases, with nodular goiter in 3 cases, and with follicular adenoma in 1 case.

• 한국가축번식학회지
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• 제4권1호
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• pp.47-74
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• 1980

The present experiments were carried out to elucidate the effects of hypophysectomy and subsequent administration of sex hormone on thyroid, adrenal gland, gonads and blood plasma components in the rat. The jresults obtained were summarized as follows: The weight of the thyroid gland of both male and female hypophysectiomzed rats decreased markedly from 7 days up to 56 days after the hypophysectomy as compared to the control group. The administration of sex hormone (6 mg of testosterone propionate to male and 6 mg of hexestrol to female) to the hypophysectomized rat gave on effect on the change in the weight of the thyroid gland. The hopophysectomy decreased the uptake of radioactive iodine in the thyroid gland in both male and female rats with time. Subsequent administration of the sex hormone caused no effect. With regard to the histological changes of the thyroid gland, the hypophysectomy caused significant changes in the gland showing a remarkable degeneration. The function of the gland seemed to disa, pp.ar almost completely on 56th day after the hypophysectomy. Upon the administration of sex hormone after the hypophysectomy, however. the epithelia of the follicle which has changed to flat from has partly returned to its functional cubicfrom and nuclei recovered as nearly as normal. These recovery were more remarkable in the female than in the male. The hypophysectomy kept causing a significant decrease in the weight of the adrenal gland in male and female rats during the period of observation (up to 56 days) as in the case of thyroid gland. The administration of sex hormone has on effect in this respect either. The hypophysectomy also caused a marked morphological change in the gland: zona fasciculata and zona reticularis were dicreased in size quichly after the hypophysectomy. The administraton of the sex hormone to the hypophysectomized rat resulted in clear distinction among the three layers of the adrenal cortex which otherwise very diffused. In the male, this phenomenum was more remarkable than in the female and the pattern of the cell arrangements and the thickening of each layer became similar to those of normal rats. The gonads of both sexes have also kept decreasing in the weight and degenerated in morpohology after the hypophysectomy. However, the degenerate follicle became enlarged after the administration of hexestrol in the female. Furthermore, the vacuoles found in interstitial cells of hypophysectomized rat disa, pp.ared after the administration of testosterone in the male and the formation of spermatocytes seemed to be recovered. Hypophysectomy also caused a gradual increase in the contents of total protein, non-protein nitrogen, total lipid, cholesterol and calcium in the blood plasma with time. The concentrations of sodium, potassium and chloride in the blood did not change after the hypophysectomy. Sex hormone caused practically no change in above tendency.