• Korean Journal of Veterinary Research
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• v.44 no.3
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• pp.329-334
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• 2004

Ischemia/reperfusion(I/R) injury of the rat testis causes germ cell death and infiltration of inflammatory cells. To investigate the mechanism of germ cell death in torsion of the rat testis, apoptosis and macrophage activation were studied using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling(TUNEL) method and immunohistochemistry in the testes of Sprague-Dawley rats subjected to 1.5 h of ischemia, followed by 0, 1, 3, 6, 12, 24, 48 and 96 h of reperfusion. Apoptotic, TUNEL-positive cells were found at the base of the seminiferous epithelia after I/R. TUNEL-positive cells were significantly increased 6 h after repair of the torsion, and there was a significant peak in apoptosis 24 h after reperfusion, as compared with normal or sham-operated controls. In contrast, histological evidence of germ cell necrosis in the seminiferous tubules was first visible 24 h after reperfusion. In the testis of sham-operated rats, ED2-positive resident macrophages were found diffusely in the interstitial space, while ED1-positive monocyte-like macrophages were rarely found. After I/R, ED1-positive cells were significantly increased beginning 12 h after reperfusion, while ED2-positive immunoreactivity did not change during the experimental period. Together, the results of this study confirmed that increased numbers of ED1-positive macrophages, but not resident ED2-positive macrophages, infiltrated the interstitial space surrounding damaged tubules and induced germcell death.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.1
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• pp.27-34
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• 2006

Sexual maturation and reproductive cycle of the bat star Asterina pectinifera were investigated by histological method. Specimens were collected monthly in Donghwari, Gosunggun, Korea from January 2004 to February 2005. The bat star was dioecious. The gonads are composed of a number of gametogenic follicles. The gonadosomatic index (GSI) of female and male were reached the maximum in July (5.72, 4.54) and the minimum in December (0.89) and January, February (0.51), and the gonad index (GI) of female and male were reached the maximum in July (3.53, 3.91) and the minimum in August (0.95) and October to December (1.0), respectively. The main spawning was from August to September. The reproductive cycle of the bat star could be divided into five stages: in the female, inactive (November-February), early active (January February), late active (March-June), ripe (July), spent and degenerative (August-November) and in the male, inactive (November-February), early active (January-March), late active (April-June), ripe (July), spent and degenerative (August-October), respectively.

• Journal of Oral Medicine and Pain
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• v.19 no.1
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• pp.45-55
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• 1994

The purpose of this study was to observe the microscopic change of salivary gland tissues, which is the cause of xerostomia in diabetic condition: for this target the author injected STZ 0.1ml/100gm b.w. on rat to produce diabetes, and than observed microscopic change in submandibular gland through the histopathologic method, obtaining as follows : 1. All of the experimental specimens suffered diabetes after injection of STZ, but the blood glucose level was irregular. 2. There were not interrelationship between the blood glucose level and microscopic change on salivary gland tissues. 3. The salivary gland changed after diabetes initiation in lapse of times; after 14 days,suffered severe destruction, however after 17 days, it was regenerated. 4. Salivary glands showed congested, destructive acini cells, and hyperplastic ductal cells as well as salivary gland duct-like structures. 5. Then were accumulation of fat granules within the cytoplasm of the acini cells on mucous gland in diabetic condition. 6. According to insulin injection, there were no more changes on salivary gland tissues, even in the accumulation of fat granules. 7. Histological changes of the serous gland were obvious more than the mucous gland in this experimental condition.

• Journal of Aquaculture
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• v.16 no.4
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• pp.245-251
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• 2003

Sexual maturation and gametogenic cycle of the hard shelled mussel, Mytilus coruscus were investigated by means of histological method. Specimens were collected monthly in Hansan Bay of south coast, Korea from January to December 2002. Sex of the clam was dioecious. The gonads are composed of a number of gametogenic follicles. The gonad index (GI) was reached the maximum in February (3.40), and the minimum in August (1.18). The monthly variation of condition index (CI) coincides with gonad index. The main spawning occurs between February and March. The gametogenic cycle of the clam could be divided into six stages: resting (August to November), multiplicative (November to December), growing (December to January), maturation (January to February), partially spent (February to April) and completely spent/degenerative stage (April to July). Sex ratio (♀/♂) was 1:0.96.

• Clinical and Experimental Reproductive Medicine
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• v.13 no.2
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• pp.195-200
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• 1986

In order to know when the cumulus cells of mouse follicles get ability to expand in vitro, the oocyte cumulus complexes obtained from different growing ages of mice were cultured in the medium containing HCG and their rate of expansion were observed and at the same time their maturation rate was examined. The growth of follicles was also checked by histological method. It was impossible to isolate the oocyte-cumulus complexes from 13 or 15 days old mouse ovaries. The oocyte-cumulus complexes collected from 17 days old mouse were partially induced to expanded by HCG, and from 19 days, most of the complexes were induced to full expansion. The rate of cumulus cell expansion by HCG and the oocyte maturation increased steadly during the growing ages to adult. Thus, the time for follicles to get competence for expansion and maturation seems to be closely related. Antral follicles were appeared from 17 days old mice and Graafian follicles were seen from 21 days old mice. The competence for cumulus expansion increased during follicle growth up to 21 days old mice.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.18 no.1
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• pp.125-134
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• 1996

Mucoepidermoid carcinomas, first reported by Volkman(1895), form 6% to 9% of all salivary tumors. Two thirds affecting the parotid gland and the remaining third, the minor glands. As we could know from its name, mucoepidermoid carcinomas originate from ductal epithelium including squamous, mucous-secreting, and undifferentiated intermediate cells. Histologically, it is classified as well-differentiated (low grade), moderately-differentiated(intermediate grade), and poorly-differentiated (high grade) types and the treatment method and prognosis are influenced from its histological grade. We have experienced two cases of mucoepidermoid carcinoma treated surgically with good results and now would like to report these with review of literatures.

• The Korean Journal of Physiology
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• v.1 no.2
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• pp.181-184
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• 1967

Hypothalamus is one of the most important center regulating voluntary and involuntary function of the body. We studied on the blood glucose and eosinophilic responses induced by electrical hypothalamic stimulation using stereotaxic apparatus. Blood glucose was measured according to Somogyi Nelson method and circulating eosinophil was counted with Hinkleman Stain. For the histological confirmation of electrode placement frozen sections were cut along electrode tract and stained with Hematoxylin-Eosin. The results obtained were as follows: 1. Anterior hypothalamic stimulation mostly leads to hypoglycemia but in some cases lead to hyperglycemia. 2. Hypothalamic stimulation leads to eosinopenia in majority cases. 3. There is no relationship between eosinopenia and hyperglycemia. 4. It appears that sympathetic and pararympathetic areas are not separate part but it coincides each other.

• The Journal of Korean Physical Therapy
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• v.3 no.1
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• pp.175-188
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• 1991

A study was performed to investigate the effect of electrical stimulation on disused rat soleus muscle, of male rat. The animal's hindlimbs were immobilized 4weeks by plaster of paris, and stimulated with E. S. T for 4weeks (20min/day) The changes on soleus were examined with histochemical, histological, and morphometric method. The results are summarized as follows. 1. Disued atrophy group from immobilization, which margin of sarcolemma and myofibril in sarcoplasm were not cleared, also degenerated from necrosis with phagocytosis. 2. The numbers of nuclear were much increased and accumulation of nuclear were finded, and relatively muscular atrophic changed. 3. Increased inflammatory cyte, also finded neutrophil and macrophage. 4. Relatively atrophic changed from severe fibrosis by incleased connectivetissue. 5. The glycogen granules were much decreased in E. S. T group. It means that electrical stimulation effected the muscle exercise. 6, The activity of the NADH-TR reaction of E. S. T. Tgroup were white muscle group are transformed into red muscle fiber than normal group. 7. These results indicate that the electrical stimulation effected to soleus also prevention and delayed muscular atrophy.

• Animal cells and systems
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• v.4 no.4
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• pp.375-379
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• 2000

The appearance of some gastrointestinal endocrine cells in the Meckel's diverticulum (MD) of the bean goose, Anser fabalis Latham was observed using specific antisera against serotonin, gastrin, cholecystokinin (CCK)-8, glucagon, secretin, somatostatin and human pancreatic polypeptide (HPP) with the peroxidase antiperoxidase (PAP) method. Among these specific antisera, serotonin-, gastrin-, CCK-8-, somatostatin- and HPP-immunoreactive cells were demonstrated in this study. Serotonin-, gastrin- and somatostatin-immunoreactive cells were detected at moderate frequency and CCK-8- and HPP-immunoreactive cells was rare and low frequencies, respectively. These immunoreactive cells were located in the superficial epithelium, intestinal crvpt and intestinal glands with spherical or spindle shaped cells having long cytoplasmic processes (open typed-cell). Mucosal layer of MD was composed of simple columnar epithelium and numerous intestinal glands. In addition, numerous lymphatic tissues were also demonstrated. In conclusion, histological profiles of MD were similar to any parts of the large intestine, especially the cecum, but the appearance, distribution and relative frequency of gastrointestinal endocrine cells were similar to those of upper parts of the small intestine. Although the exact digestive functions were unknown, the finding that the appearance, distribution and relative frequency of gastrointestinal endocrine cells in MD is similar to small intestine may be considered as distinct evidence that this organ may have some digestive functions.

• Korean Journal of Veterinary Research
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• v.61 no.3
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• pp.25.1-25.7
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• 2021

The axolotl has extraordinary regeneration capacity compared to other vertebrates. This remarkable potential has been attributed to its life-long neoteny, characterized by the exhibition of embryonic characteristics at the adult stage. A recent study provided a detailed morphological analysis of the sperm morphology of the Ambystoma mexicanum using routine and detailed histological techniques. The primary purpose of the present study is to describe a simple and inexpensive method for evaluating the morphology of axolotl sperm. In this study, spermatophore structures were collected and spread on slides and air-dried. The slides were stained with periodic acid Schiff, toluidine blue, Masson's trichrome, Giemsa, Spermac, and Diff-Quik dye for a morphological examination. The slides were coated with gold/palladium for a scanning electron microscopy examination. The sperm of the axolotl consisted of an elongated head, a neck, and a flagellum covered with an undulating membrane. The lengths of the midpiece, tail, and head were 8.575 ㎛, 356.544 ㎛, and 103.661 ㎛, respectively. In the flagellum part, the wavy membrane structure, whose function has not been explained, surrounds the tail. The data obtained from this study will constitute an important step in designing future research on the reproductive and regeneration capacity of the axolotl.