• Title/Summary/Keyword: high-temperature shift

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Characterization of dnaK Mutants in Streptococcus pneumoniae

  • Kim, Seung-Whan;Pyo, Suhk-Neung;Rhee, Dong-Kwon
    • BMB Reports
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    • v.33 no.1
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    • pp.75-81
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    • 2000
  • DnaK is a major heat shock protein and known to be highly conserved in all species. Previously, the dnaK in Streptococcus pneumoniae was cloned and the immunogenic nature characterized. In this study, dnaK mutants were generated by insertion of duplication mutagenesis and their characteristics examined. They had defective growths at all temperatures ($20^{\circ}C-42^{\circ}C$)and cell divisions, and formed filaments after a temperature shift from 30 to 42. A unique feature of the dnaK mutants of S. pneumoniae, unlike those of E. coli and B. subtilis, was the growth capability at high temperature ($42^{\circ}C$) without producing the putative GroEL. Our results suggest that DnaK may serve as a regulator and/or modifier in GroEL gene expression.

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Thermal Properties of Graphene

  • Yoon, Du-Hee;Lee, Jae-Ung;Son, Young-Woo;Cheong, Hyeon-Sik
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.02a
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    • pp.14-14
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    • 2011
  • Graphene is known to possess excellent thermal properties, including high thermal conductivity, that make it a prime candidate material for heat management in ultra large scale integrated circuits. For device applications, the key parameters are the thermal expansion coefficient and the thermal conductivity. There has been no reliable experimental determination on the thermal expansion coefficient of graphene whereas the estimates of the thermal conductivity vary widely. In this work, we estimate the thermal expansion coefficient of graphene on silicon dioxide by measuring the temperature dependence of the Raman spectrum. The shift of the Raman peaks due to heating or cooling results from both the intrinsic temperature dependence of the Raman spectrum of graphene and the strain on the graphene film due to the thermal expansion mismatch with silicon dioxide. By carefully comparing the experimental data against theoretical calculations, it is possible to determine the thermal expansion coefficient. The thermal conductivity is measured by estimating the thermal profile of a graphene film suspended over a circular hole of the substrate.

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A Study on the Sulfur-Resistant Catalysts for Water Gas Shift Reaction I. TPR Studies of $Mo/\gamma -Al_2O_3$ Catalysts

  • 박진남;김준희;이호인
    • Bulletin of the Korean Chemical Society
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    • v.19 no.12
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    • pp.1363-1368
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    • 1998
  • Mo/γ-Al2O3 catalysts were prepared by impregnation method in various conditions to identify the states of surface Mo species. TPR (Temperature-Programmed Reduction) and Raman spectroscopy were applied to analyze the surface Mo species. TPR analysis revealed that MoO3 was reduced to Mo through MoO2, the intermediate state and the increase of Mo loading enhanced the reducibility of Mo oxide till the formation of monolayer coverage. High temperature calcination induced oxygen defects in MoO3 giving their unstable states for easier reduction. Raman spectroscopy analysis showed that the increase of Mo loading induced the polymeric Mo oxide.

Reliability Assessment and Prediction of Solder Joints in High Temperature Heaters (고온히터 솔더접합부의 신뢰성 평가 및 예측)

  • Park, Eunju;Kwon, Daeil;Sa, Yoonki
    • Journal of the Microelectronics and Packaging Society
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    • v.24 no.2
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    • pp.23-27
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    • 2017
  • This paper proposes an approach to predict the reliability of high temperature heaters by identifying their primary failure modes and mechanisms in the field. Test specimens were designed to have the equivalent stress conditions with the high temperature heaters in the field in order to examine the effect of stress conditions on the solder joint failures. There failures often result from cracking due to intermetallic compound (IMC) or void formation within a solder joint. Aging tests have been performed by exposing the test specimens to a temperature of $170^{\circ}C$ in order to reproduce solder joint failures in the field. During the test, changes in IMC formation were investigated by scanning electron microscopy (SEM) on the cross-sections of the test specimens, while changes in void formation were monitored both by resistance spectroscopy and by micro-computed tomography (microCT), alternately. The test results demonstrated the void volume within the solder increased as the time at the high temperature increased. Also, the phase shift of high frequency resistance was found to have high correlation with the void volume. These results implied the failure of high temperature heaters can be non-destructively predicted based on the correlation.

Highly Sensitive Integrated Photonic Temperature Sensor Exploiting a Polymeric Microring Resonator (폴리머 마이크로링 공진기를 이용한 고감도 집적광학형 온도센서)

  • Lee, Hak-Soon;Kim, Gun-Duk;Lee, Sang-Shin
    • Korean Journal of Optics and Photonics
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    • v.19 no.3
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    • pp.224-228
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    • 2008
  • A highly sensitive integrated photonic temperature sensor was proposed and developed incorporating a polymeric microring resonator. The change in the ambient temperature was estimated by observing the shift in the resonant wavelength of the resonator induced via the thermooptic effect. For the purpose of enhancing its sensitivity, the sensor was built by implementing a polymeric resonator exhibiting a high thermooptic coefficient on a silicon substrate with a small coefficient of thermal expansion. For the range of from $20^{\circ}C$ to $30^{\circ}C$ near the room temperature, the fabricated sensor yielded a sensitivity of as high as 165 ${\pm}/^{\circ}C$ and a resolution of better than $0.1^{\circ}C$. And its performance was found to be hardly affected by the variation in the refractive index of the target analyte, which was applied to the surface of the sensor. It is hence expected that the sensor could be integrated with other refractormetric optical sensors, thereby compensating for the fatal error caused by the change in the ambient temperature.

Cultural Conditions for the Production of Organic Acid During (Aspergillus awamori var. kawachii에 의한 쌀 Koji 제조시 유기산의 생산조건)

  • 소경환
    • The Korean Journal of Food And Nutrition
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    • v.6 no.4
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    • pp.287-293
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    • 1993
  • This study was carried out to investigate the influences of cultural conditions of koji on the production of organic acid during rice-koji making by Aspergillus awamori var. kawachii which is now widely used as koji-mold in brewing Takju and Yakju in Korea. The optimum temperature for the germination of the conidia of the mold was 35'8, and the time required for germination at this temperature was 8 hours. Rapid germination occurred when the water content of steamed rice was above 40%, but germination retardation occurred markedly below 35%. The optimum cultural temperature for the production of organic acid was 32$^{\circ}C$, and the production of organic acid was markedly restricted at 36$^{\circ}C$ and 4$0^{\circ}C$. It was effective for the high production of both saccharogenic amylase and organic acid to shift the cultural temperature from initial 36$^{\circ}C$ to 32$^{\circ}C$ after 20~25 hours of cultivation. Initial water content suitable to the production of organic acid was 40% in steamed rice, but its production was markedly restricted below 30% of water content. When the quantity of conidial inoculation was too small, the production of organic acid was low in initial phase, but it was retrived at later period. Acid production was markedly restricted together with the increase in koji thickness.

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Performance optimization of 1 kW class residential fuel processor (1 kW급 가정용 연료개질기 성능 최적화)

  • Jung, Un-Ho;Koo, Kee-Young;Yoon, Wang-Lai
    • 한국신재생에너지학회:학술대회논문집
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    • 2009.06a
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    • pp.731-734
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    • 2009
  • KIER has been developed a compact and highly efficient fuel processor which is one of the key component of the residential PEM fuel cells system. The fuel processor uses methane steam reforming to convert natural gas to a mixture of water, hydrogen, carbon dioxide, carbon monoxide and unreacted methane. Then carbon monoxide is converted to carbon dioxide in water-gas-shift reactor and preferential oxidation reactor. A start-up time of the fuel processor is about 1h and CO concentration among the final product is maintained less than 5 vol. ppm. To achieve high thermal efficiency of 80% on a LHV basis, an optimal thermal network was designed. Internal heat exchange of the fuel processor is so efficient that the temperature of the reformed gas and the flue gas at the exit of the fuel processor remains less than $100^{\circ}C$. A compact design considering a mixing and distribution of the feed was applied to reduce the reactor volume. The current volume of the fuel processor is 17L with insulation.

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Reference Electrode at Molten Salt: A Comparative Analysis of Electroceramic Membranes

  • Yoon, Seokjoo;Kang, Dokyu;Sohn, Sungjune;Park, Jaeyeong;Lee, Minho;Choi, Sungyeol
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.18 no.2
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    • pp.143-155
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    • 2020
  • A reference electrode is important for controlling electrochemical reactions. Evaluating properties such as the reduction potential of the elements is necessary to optimize the electrochemical processes in pyroprocessing, especially in a multicomponent environment. In molten chloride systems, which are widely used in pyroprocessing, a reference electrode is made by enclosing the silver wire and molten salt solution containing silver chloride into the membranes. However, owing to the high temperature of the molten salt, the choice of the membrane for the reference electrode is limited. In this study, three types of electroceramic, mullite, Pyrex, and quartz, were compared as reference electrode membranes. They are widely used in molten salt electrochemical processes. The potential measurements between the two reference electrode systems showed that the mullite membrane has potential deviations of approximately 50 mV or less at temperatures higher than 650℃, Pyrex at temperatures lower than 500℃, and quartz at temperatures higher than 800℃. Cyclic voltammograms with different membranes showed a significant potential shift when different membranes were utilized. This research demonstrated the uncertainties of potential measurement by a single membrane and the potential shift that occurs because of the use of different membranes.

Hydrogen Generation Characteristics of SMART System with Inherent $CO_2/H_2$ Separation ($CO_2/H_2$ 원천분리 SMART 시스템의 수소생산특성)

  • Ryu, Ho-Jung
    • Journal of Hydrogen and New Energy
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    • v.18 no.4
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    • pp.382-390
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    • 2007
  • To check the feasibility of SMART(Steam Methane Advanced Reforming Technology) system, an experimental investigation was performed. A fluidized bed reactor of diameter 0.052m was operated cyclically up to 10th cycle, alternating between reforming and regeneration conditions. FCR-4 catalyst was used as the reforming catalyst and calcined limestone(domestic, from Danyang) was used as the $CO_2$ absorbent. Hydrogen concentration of 98.2% on a dry basis was reached at $650^{\circ}C$ for the first cycle. This value is much higher than $H_2$ concentration of 73.6% in the reformer of conventional SMR (steam methane reforming) condition. The hydrogen concentration decreased because the $CO_2$ capture capacity decreased as the number of cycles increased. However, the average hydrogen concentration at 10th cycle was 82.5% and this value is also higher than that of SMR. Based on these results, we could conclude that the SMART system can replace SMR system to generate pure hydrogen without HTS (high tempeature shift), LTS (low temperature shift) and $CO_2$ separation process.

A Development of Hardware-in-the-Loop Simulation System of Automatic Transmission for the Simulation of Shifting Characteristics (자동변속기의 변속특성시뮬레이션을 위한 HILS시스템 개발)

  • 정규홍;이교일
    • Transactions of the Korean Society of Automotive Engineers
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    • v.9 no.6
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    • pp.143-151
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    • 2001
  • During the past several years, the major interests of car manufacturers in development of automatic transmission were in durability and shift quality. However, a large number of researches for improving shift quality that are based on dynamic characteristics of shifting mechanism have been rarely adopted in the developing process because it is quite difficult to predict the shifting performance from the dynamics simulation. One of the important reasons for the difference between simulation results and experiments arises from the automatic transmission hydraulic system that consists of many valves with high order model and shows a lot different dynamics to temperature variation. In this work, hardware-in-the-loop simulation system for automatic transmission was developed f3r improving the accuracy of simulated result by combining the real-time simulation model with the real hydraulic system. The real-time simulation for automatic transmission model excluding hydraulic system is executed with TI's TMS320C31 DSP and the interfacing board which includes 12bit A/D, PWM signal generator and driver, serial driver ,etc is designed for acquiring the simulation data and signal interface with hydraulic system. We verified the proper operation and correctness of shifting result by comparing the off-line simulation result with that of HILS and experimental result which was performed on transmission dynamometer driven by electric motor.

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