• Applied Biological Chemistry
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• 제20권2호
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• pp.242-246
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• 1977

보리(Hordeum vulgare cultivar Sedohadaka)초엽에 $1{\times}10^{-5}M$ Gibbrellic acid 및 $1{\times}10^{-5}M$ Abscisic acid를 처리(處理)하여 RNase활성(活性)의 경시적(經時的) 소장(消長)을 무처리구(無處理區)와 비교(比較)하고, 핵산(核酸)의 형태(形態)를 관찰하였으며 그 결과(結果)를 요약(要約)하면 다음과 같다. 1) GA는 RNase의 활성(活性)을 억제(抑制)시키는 반면(反面)ABA는 촉진(促進)시켰다. 2) 무처리구(無處理區)에서 정상적인 식물(植物)에 비(比)하여 r-RNA의 비(比)가 낮고 r-RNA의 비(比)가 높았는데 이것은 배양(培養)중 RNase의 작용(作用)에 의한 것 같다. 3) GA는 r-RNA의 분해(分解)를 완화시켰으나 ABA는 촉진(促進)시켰는데 이것은 RNase의 활성(活性)과 관계된 것 같다. 4) GA는 DNA-RNA 복합체(複合體)의 합성(合成)을 촉진(促進)시켰으나 ABA는 이를 억제시켰다. 5) ABA에 의한 s-RNA의 증가(增加)는 r-RNA의 분해산물(分解産物) 때문이라 생각된다.

• Clinical and Experimental Pediatrics
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• 제59권2호
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• pp.100-103
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• 2016

Patients with hemolytic-uremic syndrome (HUS) can rapidly develop profound anemia as the disease progresses, as a consequence of red blood cell (RBC) hemolysis and inadequate erythropoietin synthesis. Therefore, RBC transfusion should be considered in HUS patients with severe anemia to avoid cardiac or pulmonary complications. Most patients who are Jehovah's Witnesses refuse blood transfusion, even in the face of life-threatening medical conditions due to their religious convictions. These patients require management alternatives to blood transfusions. Erythropoietin is a glycopeptide that enhances endogenous erythropoiesis in the bone marrow. With the availability of recombinant human erythropoietin (rHuEPO), several authors have reported its successful use in patients refusing blood transfusion. However, the optimal dose and duration of treatment with rHuEPO are not established. We report a case of a 2-year-old boy with diarrhea-associated HUS whose family members are Jehovah's Witnesses. He had severe anemia with acute kidney injury. His lowest hemoglobin level was 3.6 g/dL, but his parents refused treatment with packed RBC transfusion due to their religious beliefs. Therefore, we treated him with high-dose rHuEPO (300 IU/kg/day) as well as folic acid, vitamin B12, and intravenous iron. The hemoglobin level increased steadily to 7.4 g/dL after 10 days of treatment and his renal function improved without any complications. To our knowledge, this is the first case of successful rHuEPO treatment in a Jehovah's Witness child with severe anemia due to HUS.

• Nutrition Research and Practice
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• 제16권sup1호
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• pp.57-69
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• 2022

BACKGROUND/OBJECTIVES: Vitamin D is produced in the skin during sun exposure and is also ingested from foods. The role of vitamin D needs to be considered in the prevention and management of various diseases. Moreover, since the majority of Koreans spend their days indoors, becoming susceptible to the risk of vitamin D deficiency. The current study aims to prepare a basis for determining dietary reference intake of vitamin D in Korea, by reviewing the evidence against various diseases and risks. MATERIALS/METHODS: Literature published in Korea and other countries between 2014 and 2018 was prioritized based on their study design and other criteria, and evaluated using the RoB 2.0 assessment form and United States Department of Agriculture Nutrition Evidence Library Conclusion Statement Evaluation Criteria. RESULTS: Of the 1,709 studies, 128 studies were included in the final systematic analysis after screening. To set the dietary reference intakes of vitamin D based on the selected articles, blood 25(OH)D levels and indicators of bone health were used collectively. Blood vitamin D levels and ultraviolet (UV) exposure time derived from the Korean National Health and Nutrition Examination Survey were analyzed to establish the dietary reference intakes of vitamin D for each stage of the life cycle. The adequate intake levels of vitamin D, according to age and gender, were determined to be in the range of 5-15 ㎍/day, and the tolerable upper intake level was established at 25-100 ㎍/day. CONCLUSIONS: The most important variable for vitamin D nutrition is lifestyle. A balanced diet comprising foods with high contents of vitamin D is important, as is vitamin D synthesis after UV exposure. The adequate intake level of vitamin D mentioned in the 2015 Dietary Reference Intakes for Korean (KDRI) remained unchanged in the 2020 KDRI for the management of vitamin D nutrition in Koreans.

• 한국지구과학회지
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• 제18권6호
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• pp.453-463
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• 1997

제6차 교육과정 개편에 따라 1996학년도부터 고등학교에서 가르치고 있는 '공통과학'과목의 교과 내용과 7종의 교과서를 대상으로 교과서 구성 체계 및 단원별 내용의 양적 분포, 교과 내용의 구성, 탐구 활동의 내용, Schwab의 탐구 활동 수준 분류, 부록의 구성, Slayer & Bay의 목적군을 이용한 분석, Romey의 정량적 분석 등을 실시했다. 교과서의 구성 체계는 모든 교과서가 교육부가 제시한 원칙에 크게 벗어나지 않았다. 교과서의 총 면 수는 7종의 교과서가 $471{\sim}519$면으로 구성되었고 '물질', '힘', '생명', '지구' 단원간의 배분을 적절히 유지하였으며 특히 통합 단원의 의미가 있는 '에너지'와 '환경'단원을 비중 있게 다루고 있다. '공통과학'은 통합 과학의 의미를 살리려는 의도는 엿보이나 아직도 교과 내용이 화학적 통합을 완전히 이루지 못하고 있다. 우리가 일상 생활에서 부딪히는 문제들을 주제로 그 원인과 과정을 밝히는 방법으로 구성하는 것도 하나의 대안일 것이다. 교과서에서 사용하고 있는 탐구 활동의 종류는 11가지 인데 대부분 '자료해석', '실험', '조사', '토의' 등이 많이 사용되었다. 탐구활동 중 실험은 96.1%가 Schwab의 탐구 요구 수준의 제1수준에, 3.9%가 제2수준에 해당하였고, 제3수준에 해당하는 실험은 없었다. 교과서의 부록은 총 26종이 제시되어 있는데 모든 교과서가 '교과서 내용 풀이'를 포함하고 있고 기타 특정 분야에 편중되는 등 다양한 자료를 담지 못하고 있다. Slayer & Bay가 제시한 종합 연구의 목적군을 이용해 분석한 결과 교과서의 내용이 대부분 학문적 준비에 치우쳐있다. 그러나 '환경' 단원의 경우는 사회적 필요에 따른 내용을 상당히 포함하고 있다. Romey의 정량적 분석 결과는 문장, 그림 및 도표, 장 끝의 질문, 학생의 학습 활동 수 등이 $0.4{\sim}1.5$ 사이의 값으로 학생 참여를 적절히 유도하는 발견 지향적 인 것으로 조사되었다. 그러나 장의 요약은 본문 내용을 반복하는 내용으로 구성되었다. 이와 같이 공통과학 과목은 새로운 현대 사회에 부응하는 교과 목표와 체계를 지향하고 있지만 아직도 통합과학으로서의 내용과 체계를 완전히 갖추고 있지 못할 뿐만 아니라 현재 사용되고 있는 7종의 교과서가 교육 목표를 충분히 반영하지 못하고 있다. 따라서 교사의 역할이 더욱더 중요하게 되었다.

• Journal of Microbiology and Biotechnology
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• 제11권6호
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• pp.986-993
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• 2001

Translation Initiation in prokaryotes involves the formation of a 30 S preinitiation complex, in which translation initiation factors play a role in the stimulation of fMet-tRNA (fMet) binding. However, the specific function and precise mechanism of initiation factor IF1 are still unclear. One a functionally active factor with a high purity. In the present study a large quantity of active IF was rapidly purified, obtained by the overexpression of the infA gene, and then used for a functional study. The induction of infA did not appreciably affect the growth rate of the protease-deficient strain E. coli AR68 harboring the IF1 overproducing plasmid. The level of IF1 obtained was approximately $1-2\%$ of the total cell protein, which enabled the yield of highly purified IF1 (>$98\%$ pure) to be increased to 0.15 mg of IF1/g of cells. The IF1 was isolated within one day by the centrifugatioin of the ribosomal washed fraction, by ammonium sulfate fractionation, chromatography on batch of phosphocellulose, and FPLC Mono S. The overexpressed IF1 was found to be comparable to the factor isolated from normal cells, as determined by migration in NEPHGE/SDS 2-D gels. For binding of fMet-tRNA(fMet) to the 30 S ribosomal subunitis, relatively high levels of binding were obtained when IF2 was present. The addition of IF1 up to 110 pmol proportionally stimulated the binding to a variable extent. This IF1-dependent stimulation of the 30 S preinitiation complex formation demonstrated that IF1 would appear to be exclusively essential for promoting the initiation phase of protein synthesis.

• 대한간호학회지
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• 제27권3호
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• pp.639-647
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• 1997

The purpose of this study was to identify how internal and external coping resources influenced active coping in the process of stress-coping. The model was established theoretically by comparing and integrating the following theories : Stress-Coping, Self-Care, and Resourcefulness. The subjects consisted of sixty eight patients undergoing chemotherapy(experimental group 34, control group 34) at two general hospitals from January to July, 1995. The results were as follows : After self-care education, the active coping score of the experimental group was significantly higher than that of the control group. The active coping score of the high resourcefulness group was significantly higher than that of the low resourcefulness group. The interaction effect between self-care education and resourcfulness was not significant statistically. Specifically as to such scores of seeking social support, problem-oriented strategy and self care behavior, there were significantly higher in the experimental group and high resourcefulness group than in each of the other groups. Considering them both, self-care education and resourcefulness are effective nursing strategies to promote active coping including self-care. Consequently, the synthesis and testing of theories of stress-coping, self-care, and resourcefulness in this study are mostly proven to enhance the explanation and prediction of the change of active coping including self-care. Therefore the result of this study will contribute in the development of practice theory of nursing. A further study is necessary to reevaluate the interaction effect between self-care education and resourcefulness and to identify the difference between resourcefulness and self-efficacy.

• Journal of Ginseng Research
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• 제40권2호
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• pp.113-120
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• 2016

Background: The present study aimed to compare the relative abundance of proteins and amino acid metabolites to explore the mechanisms underlying the difference between wild and cultivated ginseng (Panax ginseng Meyer) at the amino acid level. Methods: Two-dimensional polyacrylamide gel electrophoresis and isobaric tags for relative and absolute quantitation were used to identify the differential abundance of proteins between wild and cultivated ginseng. Total amino acids in wild and cultivated ginseng were compared using an automated amino acid analyzer. The activities of amino acid metabolism-related enzymes and the contents of intermediate metabolites between wild and cultivated ginseng were measured using enzyme-linked immunosorbent assay and spectrophotometric methods. Results: Our results showed that the contents of 14 types of amino acids were higher in wild ginseng compared with cultivated ginseng. The amino acid metabolism-related enzymes and their derivatives, such as glutamate decarboxylase and S-adenosylmethionine, all had high levels of accumulation in wild ginseng. The accumulation of sulfur amino acid synthesis-related proteins, such as methionine synthase, was also higher in wild ginseng. In addition, glycolysis and tricarboxylic acid cycle-related enzymes as well as their intermediates had high levels of accumulation in wild ginseng. Conclusion: This study elucidates the differences in amino acids between wild and cultivated ginseng. These results will provide a reference for further studies on the medicinal functions of wild ginseng.

• Applied Biological Chemistry
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• 제19권3호
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• pp.121-129
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• 1976

우유중에 상당량 존재하는 myo-Inositol의 생성을 알아보기 위하여 3% SE-52를 충진시킨 gas-liquid chromatography에 의해 myo-Inositol의 정량을 행하고 이 myo-Inositol의 유래를 feeding 실험과 $(^{14}C)-glucose$와 (3H)-myo-Inositol을 쥐에 주사한 실험으로 알아 븐 결과 다음과 같은 결과를 얻었다. 1. 쥐우유를 G.L.C. 방법으로 분석하여 우유 100gm.당 61-91mg.의 myo-Inositol (free)을 얻었다. 2. Feeding 실험 결과 우유 myo-Inositol의 85%는 diet에서 흡수되었고 나머지는 mammary gland에서 합성된 것으로 나타났다. 3. $(^{14}C)-glucose$에서 $(^{14}C)-myo-Inositol$ 생성은 lactose의 생성과 비슷하게 한시간이내에 최고에 달하였다. 4. $(^3H)-myo-Inositol$을 lactating rat에 주사하여 실험한 결과 우유 myo-Inositol의 대부분은 혈액 myo-Inositol에서 유래되었다. $(^{14}C)-glucose$를 주사한 후 우유중에 나타난 myo-Inositol의 비방사능(比放射能)은 4시간 후에 혈액 glucose의 8%였다.

• Asian-Australasian Journal of Animal Sciences
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• 제22권5호
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• pp.659-666
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• 2009

Metabolizable protein (MP) supply and amino acid balance in the intestine were manipulated through selection of highly digestible rumen-undegradable protein (RUP) sources and protected methionine (Met) supplementation. Four ruminallycannulated, multiparous Holstein cows averaging 193${\pm}$13 days in milk were used in a 4${\times}$4 Latin square design to assess N utilization and milk production responses to changes in RUP level, post-ruminal RUP digestibility and protected Met supplementation. Treatments were A) 14.0% crude protein (CP), 8.0% rumen degradable protein (RDP) and 6.0% RUP of low intestinal digestibility (HiRUP-LoDRUP); B) 14.1% CP, 8.1% RDP and 6.0% RUP of high intestinal digestibility (HiRUP-HiDRUP); C) 13.1% CP, 7.9% RDP and 5.2% RUP of high intestinal digestibility (LoRUP-HiDRUP), and D) 13.1% CP, 7.9% RDP and 5.2% RUP of high intestinal digestibility plus rumen escape sources of Met (LoRUP-HiDRUP+Met). Experimental diets were formulated to have similar concentrations of RDP, net energy of lactation ($NE_L$), neutral detergent fiber (NDF), acid detergent fiber (ADF), calcium, phosphorus and ether extract using the NRC model (2001). Results showed that dry matter intake (DMI), production of milk fat and protein were similar among treatments. Milk production was similar for diet HiRUP-LoDRUP, HiRUP-HiDRUP and LoRUP-HiDRUP+Met, and significantly higher than diet LoRUP-HiDRUP. Milk fat and protein percentage were higher for cows receiving HiDRUP treatments, with the greatest increases in the diet LoRUP-HiDRUP+Met. There was no significant change in ruminal pH, $NH_3g-N$ and volatile fatty acid (VFA) concentration among all treatments. Apparent digestibility of dry matter (DM), CP, NDF and ADF and estimated bacterial CP synthesis were similar for all treatments. Nitrogen intakes, blood and milk urea-N concentrations were significantly higher for cows receiving HiRUP diets. Urine volume and total urinary N excretion were significantly lowered by LoRUP diets. Lowering dietary RUP level while supplementing the highly digestible RUP source with rumen escape sources of Met resulted in similar milk production, maximal milk fat and protein concentration and maximum N efficiency, indicating that post-ruminal digestibility of RUP and amino acid balance in the small intestine can be more important than total RUP supplementation.

• 대한전자공학회논문지SD
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• 제43권12호
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• pp.23-32
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• 2006

다층 고속 디지털 보드에 대한 빠르고 정확한 전압 버스 설계 방법은 정확하고 정밀한 고속 보드에 전원 공급망 설계 방법을 위해 고안되었다. FAPUD는 PBEC(Path Based Equivalent Circuit)모델과 망 합성 방법의 두 중요 알고리즘을 기반으로 구성된다. PBEC 모델 기반의 회로 레벨의 2차원 전원 분배 망의 전기적 값으로부터 lumped 1차원 회로 모델로 간단한 산술 표현들을 활용한다 제안된 PBEC 기반인 회로 단계 설계는 제안한 지역 접근법을 이용해 수행된다. 이 회로 단계 설계는 온칩 디커플링 커패시터의 크기, 오프칩 디커플링 커패시터의 위치와 크기, 패키지 전압 버스의 유효한 인덕턴스를 직접 결정하고 계산한다. 설계 출력에 따라 모든 디커플링 커패시터가 포한된 lumped 회로 모델과 전압 버스의 레이아웃은 FAPUD 방법을 이용한 후 얻을 수 있다. 미세조정 과정에서, I/O Switching에 의해 덧붙여진 Simultaneous Switching Noise(SSN)를 고려한 보드 재 최적화가 수행될 수 있다 이는 전원 공급 잡음에 I/O 동작 효과가 lumped 회로 모델을 가지고 전 동작 주파수 범위에 대해 추산될 수 있기 때문이다. 게다가 만약 설계에 조정이 필요하거나 교체해야 한다면, FAPUD 방법은 다른 전면 설계변경 없이 디커플링 커패시터들을 대체하여 설계를 수정하는 것이 가능하다. 마지막으로 FAPUD 방법은 전형적인 PEEC 기본설계 방법과 비교해 정확하고 FAPUD 방법의 설계 시간은 전형적인 PEEC 기본 설계 방법의 시간보다 10배가 빠르다.