• Title/Summary/Keyword: high range

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Phylogentic Position, Pigment Content and Optimal Growth Condition of the Unicellular Hydrogen-Producing Cyanobacterial Strains from Korean Coasts (한국 연안산 단세포성 수소생산 남세균 종주들의 분류계통, 색소함량 및 최적성장 환경)

  • PARK, JONG-WOO;KIM, JU HEE;CHO, AE-RA;JUNG, YUN-DUK;KIM, PYOUNG JOONG;KIM, HYUNG-SEOP;YIH, WONHO
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.20 no.3
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    • pp.131-140
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    • 2015
  • To set up unicellular cyanobacterial strains with photo-biological $H_2$ production potential, live samples were repeatedly collected from 68 stations in the coastal zone of Korea for the four years since 2005. Among 77 cyanobacterial strains established six (KNU strains, CB-MAL002, 026, 031, 054, 055 and 058) were finally chosen as the excellent strains for $H_2$ production with $H_2$ accumulation over 0.15 mL $H_2\;mL^{-1}$ under general basic $H_2$ production conditions as well as positive $H_2$ production for more than 60 hr. To explore optimum procedures for higher $H_2$ production efficiency of the six cyanobacterial strains, the inter-strain differences in the growth rate under the gradients of water temperature and salinity were investigated. The maximum daily growth rates of the six strains ranged from 1.78 to 2.08, and all of them exhibited $N_2-fixation$ ability. Based on the similarity of the 16S rRNA sequences, all the test strains were quite close to Cyanothece sp. ATCC51142 (99%). The six strains, however, were grouped into separate clades from strain ATCC51142 in the molecular phylogeny diagram. Chlorophyll- a content was 3.4~7.8% of the total dried weight, and the phycoerythrin and phycocyanin contents were half of those in the Atlantic strain, Synechococcus sp. Miami BG03511. The growth of the six strains was significantly suppressed at temperatures above the optimal range, $30{\sim}35^{\circ}C$, to be nearly stopped at $40^{\circ}C$. The growth was not inhibited by high salinities of 30 psu salinity in all the strains while strain CB055 maintained its high growth rate at low salinities down to 15 psu. The euryhaline strains like CB055 might support massive biotechnological cultivation systems using natural basal seawater in temperate latitudes. base seawater. The biological and ecophysiological characteristics of the test strains may contribute to designing the optimal procedures for photo-biological $H_2$ production by unicellular cyanobacteria.

Development of Analysis Method of Gardenia Yellow as Natural Colorants and Content Survey in Commercial Foods by HPLC (HPLC를 이용한 천연착색료인 치자황색소의 분석법 개발 및 시판 식품중 함유량조사)

  • Kim, Hee-Yun;Kim, So-Hee;Hong, Ki-Hyoung;Lee, Chul-Won;Kim, Kil-Saeng;Ha, Sang-Chul;Jo, Jae-Sun
    • Korean Journal of Food Science and Technology
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    • v.31 no.4
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    • pp.945-951
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    • 1999
  • A simple and practical method for the determination of gardenia yellow in foods was developed. In this method, analysis of gardenia yellow in food products has been carried out by the detection of crocetin and/or geniposide as indicator compounds. As a new analytical method for gardenia yellow, we adopted crocetin, which is produced from colored components of gardenia yellow by alkaline hydrolysis, as an indicator compound. The analysis of gardenia yellow was performed by reverse phase high performance liquid chromatography using a Capcell pak $C_{18}$ column at wave length 240 nm (geniposide) and 435 nm (crocetin). The recovery rates of geniposide and crocetin were found to be 93.4% and 87.8% for Dan Mu Ji, 90.2% and 85.9% for milk, 92.8% and 86.5% for snack, respectively. With this method, the range of crocetin and geniposide contents $({\mu}g/g)$ were as follows: $ND{\sim}1.7$ and $ND{\sim}14.1$ for Dan Mu Ji, $ND{\sim}0.2$ and $ND{\sim}13.6$ for milk, $ND{\sim}1.6$ and $ND{\sim}0.9$ for snack, respectively. The detection limits of crocetin and geniposide were 0.07 ${\mu}g/g$ and 0.05 ${\mu}g/g$, respectively.

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Characteristics of Pinus densiflora-Dominant Community on the Mountain Ridges of the Nakdong-Jeongmaek - Focusing on the Baekbyeongsan, Chilbosan, Baegamsan, Unjusan, Goheonsan - (낙동정맥 마루금 일대의 소나무우점군락 특성 - 백병산, 칠보산, 백암산, 운주산, 고헌산을 중심으로 -)

  • Kang, Hyun-Mi;Park, Seok-Gon;Lee, Sang-Cheol
    • Korean Journal of Environment and Ecology
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    • v.30 no.4
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    • pp.751-761
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    • 2016
  • Since the position of Nakdong-Jeongmaek stretches from south to north, it shows clear changes of the climate compared to other mountain ranges. This study aimed to analyze the characteristics of vegetation in Pinus densiflora-dominant community (PDDC), which typically appeared on the ridge of this area, by each region and community. For this, the PDDCs in five representative areas of Nakdong-Jeongmaek (Baekbyeonsan, Chilbosan, Baegamsan, Unjusan and Goheonsan) were investigated. Communities were divided into four groups based on the characteristics of the growing environment for indicator species among the low vegetation, while the pinus densiflora was dominant on the canopy according to TWINSPAN. In community I, Fraxinus sieboldiana and Rhododendron schlippenbachii were dominant and there was a typical vegetation on the mountain ridge. In community II that was located on the upper slope, there was abundant inflow of sunlight to the inside of the forest which resulted from the disturbance, and it formed the secondary forest where deciduous broadleaf tree species such as Rhus cotinus appeared. Compared to the other communities, community III had a wider range of growing environment and various species of deciduous broadleaf trees appeared, so that changes in the vegetation structure were expected in the future. Finally, with the appearance of Salix hallaisanensis and Betula davurica, community IV are presumed to be located at a relatively high region with bountiful soil moisture. Reviewing by regions, PDDCs in Unjusan and Goheonsan were near big cities such as Pohang-si and Ulsan-si, so that the artificial disturbance resulted in high level of species diversity on a stage of vegetation development and the ages of Pinus densiflora were relatively young. On the other hand, PDDCs showed a stable vegetation status with low level of species diversity in other regions, and the ages of Pinus densiflora were relatively old.

Optimal Transplanting Time for 'Saemimyeon' Production in Youngnam Province (영남지방에서 쌀면용 새미면 최대생산을 위한 이앙적기 설정)

  • Bae, Hyun Kyung;Hwang, Jung Dong;Seo, Jong Ho;Kim, Sang Yeol
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.63 no.3
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    • pp.205-209
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    • 2018
  • 'Saemimyeon,' a Tongil-type rice variety (Indica x Japonica), containing high amylose content, is suitable for rice noodle production. Currently, the major parts of the rice processing industry that include products such as rice flour and noodles are expected to partially replace the wheat flour market. The volume of the rice noodle market is growing and can contribute to the rice surplus problems and farmer's income. This study was carried out to promote productivity of 'Saemimyeon' by finding the most suitable transplanting times in Youngnam Province. The transplanting times were May $10^{th}$, May $17^{th}$, May $24^{th}$, May $31^{th}$, June $7^{th}$, and June $14^{th}$ and the planting distance was $30{\times}12cm$. The field experiment was conducted in the Miryang region (southern plain region of Korea) from 2015 to 2017. Our results suggested that the optimum transplanting dates were from May $17^{th}$ to May $31^{th}$, which resulted in an average yield of 750 kg/10 a. The average grain filling rate before May $31^{th}$ was more than 83% and it declined to 75% after June $7^{th}$. The average temperature range from heading to harvesting time was $21{\sim}25^{\circ}C$ and the estimated optimal temperature was $24^{\circ}C$, which is similar to that of May $24^{th}$ by regression equation. It is suggested that low temperature at seed maturation time caused the lower grain filling rate, and therefore 'Saemimyeon' needed to be transplanted before May $31^{th}$ for higher productivity. Estimated optimal transplanting time based on temperature was 1 ~ 2 weeks earlier than the optimum transplanting time for common japonica rice cultivars in Youngnam Province.

A Study on Decision-Making Processes of Organic Foods (무공해식품의 구매의사결정에 관한 연구)

  • NamKung, Sok
    • Journal of the Korean Society of Food Culture
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    • v.9 no.4
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    • pp.379-394
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    • 1994
  • The purpose of this study was to identify the correlation between the factors influencing on housewives' decision-making processes of organic foods and the relating variables, and the 5 stages of decision-making processes of the EBK model is utilized in this study. The sample was selected from 411 housewives living in Seoul from 1st of September through 20th of September, 1993. Frequency, Percentage, Mean, Factor analysis, One-way ANOVA, Duncan's multiple range test, t-Test, Correlation, Multiple regression analysis and Path analysis were measured. Major results are as follows: 1. Purchasing motivation of the organic foods were in order of the health care, nutritive value and taste care. 2. The major informations source for the knowledge of organic foods were in order of TV/radio, newspaper/magazine, recommendations informations and advice through a family/friends/acquaintances. 3. Evalution criteria in shopping of organic foods, the total degree of consideration over the purchasing factors of organic foods was fairly high level: consumers thought much of the sanitation/freshness, nutritive value and the food safety. In this regard opinion leaders was dominantly mass media. Consumers have a tendency to purchase organic foods in consideration of their children and husband. 4. Major place to purchase organic foods are super markets and department stores. And When shopping organic foods, housewives by all means confirm the check points in their own mind, which were expiry date, manufactured date and packing condition, but unexpectedly manufactured company was out of concern. 5. Housewives usually satisfy with decision after purchasing organic foods, while they were fairly unsatisfied with the price, quality, incomplete description for ingredients and manufactured date. 6. The variables influencing to the sincerity when selecting the most desired organic foods is how be cares about the natural freshness of the foods and the types of residents in order. Another interesting tendency is the richer they are very considerate to decide. It is to say the people who cares more about the natural freshness is the sincerer when making decision and also the class who lives in the apartment house enjoying high income do not easily accept the product quality.

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Productivity Affected by Various Disease Conditions in Bovine (소의 질병감염이 생산성에 미치는 영향)

  • 이성재;이후식;노수일;김길수;이주묵
    • Korean Journal of Veterinary Service
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    • v.17 no.3
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    • pp.227-246
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    • 1994
  • Present investigations were undertaken in order to clarify the clinical status of Korean native cattle and dairy cattle(holstein). Blood, Feces and urine samples were collected from 247 Korean native cattle(222 adult and 25 calf), 224 dairy cattle(211 adult and 13 calf) at Chonbuk area and analyzed for clinical, serum chemical, hematological and urinary findings. In addition, we were examined the infection rate of Theileriosis, internal patasite and ring worm. The mean value for each component was calculated by statistical analysis using Excel computer program. From these investigations the following results were obtained. The mean values for RBC, PCV and etc in 433 adult cow(Korean native cattle and dairy cattle) were similar with other reports. But the mean values for MCHC of all species were lower than normal. Adult Korean native cattle and adult dairy cattle which showed hematologically normal levels were only 9.01% and 9.48%, respectively. Ahnormally high values for PCV, RBC and Hb were recorded in 7.66% of adult Korean native cattle, 20% of Korean native calf, 15.38% of dairy calf. Adult Korean native cattle and adult dairy cattle which showed hematologically anemia were 4.95% and 19.43% respectively, but all in Korean native calf and dairy calf showed normal values. Adult Korean native cattle, adult dairy cattle, Korean native calf and dairy calf which showed normal serum protein level were 84.0%, 90.8%, 50% and 44.4%, respectively. In present investigations, 50% of Korean native calf and 55.6% of dairy calf were decreased serum protein values under normal range. These abnormally decreased serum protein values mean the shortage of antibody, and these have a possibility to occrus to pneumonia and diarrhea. From these results, the economical loss caused by pneumonia was calculated as 124, 038, 833 won in the KNC and 742, 703, 430 won in the dairy calf rearing in Chonbuk area. Calculated economical loss caused by enteritis was 56, 658, 690 won in Korean native cattle. 476, 775, 799 won in dairy calf and the total loss amount to 533, 434, 488 won in Chonbuk area. Abnormally high values($21.7{\pm}4.0mg/dl) for serum calcium were recorded 49.6% in dairy cattle. The mean values of serum total cholesterol were $170.8{\pm}99.8mg/dl in Korean native cattle, $196.0{\pm}40.6mg/dl$ in Korean native calf, $202.9{\pm}86.0mg/dl$ in adult dairy cattle and $289.4{\pm}97.5mg/dl$ in dairy calf. The infection rate of internal parasite were as follows; adult Korean native cattle:21.2%, Korean native calf:80%, adult dairy cattle:67.8%. The estimated economical loss caused by internal parasites infection were 1, 120, 855, 837 won in Korean native calf, 4, 994, 959, 405 won in adult Korean native cattle, 3, 334, 751, 066 won in adult holstein, and the total loss amount to 9, 450, 566, 308 won. The infection rate of theileriosis were 1.4% in Korean native cattle and 6.6% in dairy cattle. The presumed Economical loss by T. sergenti infection were 154, 408, 482 won in Korean native cattle and 171, 577, 237 won in dairy cattle rearing at Chonbuk area. The infection rat of ringworm were 0.5% in Korean native cattle, 0.9% in adult dairy cattle and 7.7% in dairy calf. The presumed economical loss by dermatomycophyte were 12, 061, 532 won in Korean native cattle, 16, 895, 403 won in dairy cattle, and the total estimated loss amount to 28, 955, 935 won a year in Chonbuk area. The infection rate of ringworm were 0. 5% in Korean native cattle, 0.9% in adult dairy cattle and 7.7% in dairy calf. The presumed economical loss by dermatomycophyte were 12, 061, 532 won in Korean native cattle, 16, 895, 403 won in dairy cattle, and the total estimated loss amount to 28, 955, 935 won a year in Chonbuk area.

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Identification of the Transmissive Fractures in the Vicinity of waterway Tunnel (도수로터널 주변 지역의 지하수 유동성 단열 규명)

  • 이병대;이인호;추창오;함세영;성익환;황세호
    • Journal of Soil and Groundwater Environment
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    • v.7 no.3
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    • pp.33-44
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    • 2002
  • A field technique for assessing the transmissive fractures in an aquifer was applied to a fractured rock formation in Youngchun area Korea. Geological mapping and detailed acoustic borehole teleview(BHTV) logging were performed to obtain information about the fractures. The study area consists predominantly of two types of fractures. The fracture sets of low angle partings such as bedding and sheeting plains have strike N70-80$^{\circ}$W, 25$^{\circ}$-30$^{\circ}$SW and N3S$^{\circ}$W, 12$^{\circ}$NE, respectively. In areas of high fractures, on the other hand, the major fracture sets show strike N80$^{\circ}$W and dip 70$^{\circ}$-85$^{\circ}$SW, N10$^{\circ}$E.85$^{\circ}$SE in sedimentry rocks, N40-50$^{\circ}$E.85$^{\circ}$SE/85$^{\circ}$NE, N70$^{\circ}$E.80$^{\circ}$SE, and N7$^{\circ}$-75$^{\circ}$W.80$^{\circ}$SW in granites and volcanic rocks. Injection tests have been performed to identify discrete production zones and quantify the vertical distribution of hydraulic conductivity. The calculated hydraulic conductivities range from 3.363E-10 to 2.731E-6, showing that the difference between maximum and minimum value is four order of magnitude. Dominant section in hydraulic conductivity is extensively fractured. Geophysical logging was carried out to clarify characterization of the distribution of fracture zones. Transmissive fractures were evaluated through the comparison of the results obtained by each method. The temperature logs appeared to be a good indicator that can distinguish a high transmissive fractures from a common fractures in hydraulic conductivity. In numerous cases, evidence of fluid movement was amplified in the temperature gradient log. The fracture sets of N70-80$^{\circ}$W.60-85$^{\circ}$NE/SW N75-80$^{\circ}$W.25-30$^{\circ}$SW, N50-64$^{\circ}$W.60-85$^{\circ}$NE, N35-45$^{\circ}$E.65-75$^{\circ}$SE, and N65-72$^{\circ}$E.80$^{\circ}$SE/60$^{\circ}$NW were idenfied as a distinct transmissive fractures through the results of each tests.

Characteristics of Phosphorus Accumulation in Organic Farming Fields (유기농업실천농가 포장내 인산의 분포특성)

  • Kim, Pil-Joo;Lee, Sang-Min;Yoon, Hong-Bae;Park, Yang-Ho;Lee, Ju-Young;Kim, Suk-Chul;Choe, Suk-Chul
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.4
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    • pp.234-241
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    • 2000
  • Organic farming (OF) is regarded as alternative farming types against general farming system for sustainable agriculture, recently. However, there is little information on effects of OF on soil properties and watershed condition. In order to determine the effects of OF on soil properties, 36, 10 and 8 sites of organic farming (OF) fields of plastic film houses, paddy and orchard were selected in the national scale, respectively, to evaluate their chemical properties and phosphorus distributing characteristics. The average organic matter (OM) contents in organic farming fields were with $44g\;kg^{-1}$ in plastic film houses, $26g\;kg^{-1}$ in paddies and $39g\;kg^{-1}$ in orchard soils higher than the average OM contents in conventional farming (CF) soils. Available phosphates were accumulated to 986 in plastic film house soils and $754mg\;kg^{-1}$ in orchard soils, respectively, over the optimum range. Furthermore, total P (T-P) reached to $2.973mg\;kg^{-1}$ in plastic film houses and $2303mg\;kg^{-1}$ in orchards in OF soils. It could be attained by applying repeatedly low N/P ratio of manure-based compost. In two types of soils inorganic P was dominant with the ratio of 62~80% of T-P, and then residual and organic Ps followed. However. residual-P was dominant in paddy soils with the rate of 50% of T-P. Fractionation of soil extractable P showed that Ca-P was dominant with about $1,330mg\;kg^{-1}$ in upland soils in OF fields, which is affected by high soil pH of over 6.0. However. Fe-P of extractable P was dominant in paddy soils. Water-soluble P was very high with 65 and $26mg\;kg^{-1}$ in plastic film house and orchard soils in OF. From this results. OF regarded as an environment-friendly farming system may cause serious soil deterioration by accumulated phosphorus and may also cause water pollution.

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Bioecological Studies in the Upwelling Area of Cheju Island. ( I ) - Upwelling Phenomenon and Chemical Properties of Seawater in the Southwestern Coastal Area of Cheju Island (제주도 주변 용승역의 생물생태학적 기초연구 ( I )- 제주도 남서부 연안해역의 해수화학적 특성과 용승현상)

  • KANG Tae-Youn;CHOI Young-Chan;Go You-Bong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.5
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    • pp.603-613
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    • 1996
  • Hydrographic conditions (temperature, salinity, dissolved oxygen), nutrients, chlorophyll-a and suspended solid have been studied in the southwestern coastal area of Cheju Island from April 1993 to March 1994. Vertical profile of temperature, salinity and chemical properties (nutrients, chlorophyll-a) distribution in th southwestern sea of Cheju Island showed a upwelling feature. Although it was not clear in winter season, it seems to continued through out the year. In the surface water at the upwelling areas, the ranges of dissolved oxygen, nitrate, phosphate and silicate was $3.30\~8.43\;ml/l,\;0\~7.12{\mu}g/l,\;0.03\~1.75{\mu}g-at/l\;and\;2.75\~22.32\;{\mu}g-at/l$l, respectively. Nutrients was higher in the shore water than in the offshore water, because sufficient supply of nutrients from the bottom water by coastal upwelling. In November, especially high concentration silicate was observed at all the stations and depth in the study area. At all station of bottom water (down to the depth of 60 meter), concentration value of dissolved of gen was as high as 8 ml/l. Mean values of N/P was 8.0, lower than Redifield ratio of 16. The mean values of Si/P was observed to 46.3 in southwest of Cheju Island. Concentration of chlorophyll-a was in the range of $0.04\~2.36\;{\mu}g/l$. Concentration of chlorophyll-a in surface orator at all station was especially higher in spring than in other seasons. Mean concentration value of suspended solids was 3.14 mg/l $(0.75\~8.47\;mg/l)$. Ratio of the volatile suspended solids to the suspended solids was higher in the inshore water $(53\%)$ than in the offshore water $(46\%)$, and higher in the surface water than in the bottom water.

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THE EFFECT OF WASHING PHOSPHORIC ACID ETCHANT ON SHEAR BOND STRENGTH OF AN ORTHODONTIC ADHESIVE (인산 부식액의 수세가 교정용 접착레진의 전단결합강도에 미치는 영향)

  • Kim, Hee-Kyun;Lee, Ki-Soo;Park, Young-Guk
    • The korean journal of orthodontics
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    • v.26 no.5 s.58
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    • pp.497-507
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    • 1996
  • The aim of present study in vitro was to evaluate and compare the effects of different washing times of enamels etched with low phosphoric acid solution which makes unsoluble salts and etched but contaminated with saliva on shear bond strength of an orthodontic adhesive to enamel, and to observe the washing effect on the etched enamel surface by scanning electron microscope. All brackets were bonded with Mono-$Lok2^{TM)}$) on the labial surface of extracted human bicuspids after etching with $20w/w\%\;and\;37w/w$ and phosphoric acid solution for 60seconds and then washing for 0,5,10 and 20seconds respectedly. After etching with $37w/w\%$ phosphoric acid solution and contaminating with saliva for 30seconds and then washing for 0,5,20 and 30seconds and re-etching for 10seconds. After 24hours passed in the $37^{\circ}C$ water bath, the shear bond strengths were measured on Universal Test Machine. The data were evaluated and tested by ANOVA and Duncan's multiple range test, and those results were as follows. 1. There was no significant differences between (p>0.05) shear bond strength of bonded brackets with 5, 10, 20seconds washing etched enamel using $37{\%}w/w{\%}$ phosphoric acid solution. 2. The shear bond strength of bonded brackets with $20w/w\%$ phosphoric acid and then washing for 5seconds showed bonded strength durable to occlusal force but its coefficiency score was high and etched surface was not cleaned completely and therefore it was assumed that its clinical application is not applicable. 3. There was no significant differences between (p>0.05) shear bond strengths of bonded brckets with washing for 5seconds etched enamel using $37w/w\%$ phosphoric acid solution and 10,20 seconds washing etched enamel using $20w/w\%$ phosphoric acid solution. 4. The shear bond strength of washing for 5seconds etched enamel which was contaminated with saliva showed sufficient bonded strength durable to occlusal force but its coefficiency score was high and therefore its clinical application was not applicable. 5. After etching, the sample contaminated with saliva showed the sufficient shear bond strength even washing 20seconds without re-etching.

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