• 물과 미래
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• 제17권2호
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• pp.85-97
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• 1984

Measuring the river flow and water quality in the midstream of the Nakdong River, the natural purification status in examined through the analyses of the elements which affect the variation of dissolved oxygen, and DO model is evaluated to the midstream reach of the river. The major results of this study are as follows; the pruification factor of the of the river is relatively high, it is worried over eutrophication considering much production of algae, and it is evaluated that important factor affecting the DO value computed by the proposed DO model are in order of reaeration coefficient, carbonaceous BOD and deoxygenation constant.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.719-722
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• 2001

Silk proteins from Nephila clavipes are fibrous proteins containing repetitive sequences with both crystalline and amorphous domains. In order to obtain high-level production of silk protein, the synthetic genes had 16 contiguous units of the consensus repeat sequence of the silk protein were expressed in Escherichia coli BL21(DE3) under the strong inducible T7 promoter. For production of recombinant silk protein in large amounts, pH-stat fed-batch cultures were carried out. The recombinant silk protein was produced as soluble forms in E. coli, and the recombinant silk protein content was as high as 11% of the total protein. When cells were induced at $OD_{600}$ of 60, the amount of silk protein produced was 6.49 g/L. After simple purification steps, 9.2 mg of silk protein that was more than 80% pure was obtained from a 50 mL culture, and the recovery yield was 26.3%.

• 한국식품영양학회지
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• 제9권3호
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• pp.242-246
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• 1996

High viscous hyaluronic acid complex from Klebsiella sp. L-10 NTG 50 mutant was purified by two-phase extraction system using PEG-K2HP04 and its physicochemical properties were Investigated. Viscosity of the purified hyaluronic acid complex was decreased as temperature and salts concentration were Increased and also showed low viscosity at below pH 5.0 and above pH 11.0. Hardness, cohesiveness and adhesiveness of the purified hyaluronic acid complex were 1, 20kg, 1.91 and 0.62, respectively. Water holding capacity was 6.9ml per gram of the purified hyaluronic acid complex powder.

• Fisheries and Aquatic Sciences
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• 제15권3호
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• pp.191-195
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• 2012

We report here the use of high-speed countercurrent chromatography (HSCCC) in the preparative isolation and purification of the bioactive component, fucosterol, from Pelvetia siliquosa. A crude extract was obtained by ultrasonic extraction of powdered P. siliquosa using methylene chloride and was then subjected to separation and purification by HSCCC, coupled with evaporative light-scattering detection. Preparative HSCCC was performed successfully using a two-phase solvent system, n-heptane:methanol (3:2, v/v), to obtain 10.96 mg fucosterol with 96.8% purity from 50 mg of crude extract; the recovery rate was approximately 90.5%.

• 한국환경농학회지
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• 제26권1호
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• pp.25-35
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• 2007

인공습지나 수변녹지 및 완충 저류지와 같은 수질정화시설을 조성하는데 이용할 수 있는 수생식물을 선발하고자 낙동강 하류에 자생하는 수생식물과 외래 수생식물, 습생작물을 대상으로 부영양화 성분(T-N, T-P) 및 EC 관련 무기성분에 대한 정화능력을 검정하였다. 수생식물의 생육기간 동안 $225{\sim}444\;L\;m^{-2}$ 범위의 생활오폐수를 사용하였으며, 건물생산량이 많았던 벼와 부들, 줄, 비자루국화, 율무, 털물참새피 등이 350 L $m^{-2}$ 이상으로 가장 많은 생활하수를 소모하였다. 질소와 인의 체내흡수량과 실제 정화능력(정화효율 및 정화량) 및 수확 후 토양을 분석한 결과, 초장이 크면서 건물량도 많았던 벼, 비자루국화, 부들, 율무, 털물참새피, 줄 등이 부영양화성분에 대한 우수 정화식물로 선발되었다. 무기성분 중 K에 대한 정화력은 부영양화성분의 우수 정화식물로 선발된 수생식물과 더불어 물상추와 부레옥잠이 높은 결과를 보였다. EC와 관련이 높은 Ca와 Na, Cl에 대한 정화효과가 우수한 수생식물로는 물상추와 부레옥잠, 털물참새피가 선발되었으며, 특히 털물참새피는 $SO_4$에 대한 정화효과도 뛰어났다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.557-557
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• 2003

• 한국자기학회:학술대회 개요집
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• 한국자기학회 2008년도 Asian Magnetics Conference
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• pp.283.1-283.1
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• 2008

• BMB Reports
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• 제44권4호
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• pp.279-284
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• 2011

The glutathione S-transferase (GST) system is useful for increasing protein solubility and purifying soluble GST fusion proteins. However, purifying half of the GST fusion proteins is still difficult, because they are virtually insoluble under non-denaturing conditions. To optimize a simple and rapid purification condition for GST-pyruvate kinase muscle 2 (GST-PKM2) protein, we used 1% sarkosyl for lysis and a 1 : 200 ratio of sarkosyl to Triton X-100 (S-T) for purification. We purified the GST-PKM2 protein with a high yield, approximately 5 mg/L culture, which was 33 times higher than that prepared using a conventional method. Notably, the GST-high-temperature requirement A2 (GST-HtrA2) protein, used as a model protein for functional activity, fully maintained its proteolytic activity, even when purified under our S-T condition. This method may be useful to apply to other biologically important proteins that become highly insoluble in the prokaryotic expression system.

• Archives of Pharmacal Research
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• 제21권3호
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• pp.291-297
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• 1998

Cop protein has been overexpressed in Escherichia coli using a T7 RNA polymerase system. Purification to apparent homogeneity was achieved by the sequential chromatography on ion exchange, affinity chromatography, and reverse phase high performance liquid chromatography system. The molecular weight of the purified Cop was estimated as 6.1 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). But the molecular mass of the native state Cop was shown to be 19 kDa by an analytical high performance size exclusion chromatography, suggesting a trimer-like structure in 50 mM Tris-HCI buffer (pH 7.5) containing 100 mM NaCl. Cop protein Was calculated to contain $39.1% {\alpha}-helix, 16.8% {\beta}-sheet$, 17.4% turn, and 26.8% random structure. The DNA binding property of Cop protein expressed in E. coli Was preserved during the expression and purification process. The isoelectric point of Cop was determined to be 9.0. The results of amino acid composition analysis and N-terminal amino acid sequencing of Cop showed that it has the same amino acid composition and N-terminal amino acid sequence as those deduced from its DNA sequence analysis, except for the partial removal of N-terminal methionine residue by methionyl-aminopeptidase in E. coli.

• 한국결정성장학회지
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• 제29권6호
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• pp.276-282
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• 2019

본 연구의 목적은 공기청정기의 극세필터(프로필터), 집진필터, 헤파(HAPA-High Efficiency Particulate Air)필터, 탈취필터 등을 사용하는 일반적인 필터의 구조를 분석하고 초미세먼지, 유해가스 및 살균 정화성능을 향상시킬 수 있는 새로운 형태의 공기정화 필터 디자인을 제시하는 것이다. 기존 단계적 여과 필터 형태에 광촉매 필터 시스템을 추가하는 방법으로 연구하였으며, 광촉매 필터는 좌우측의 UV-LED 설치 프레임과 광촉매 코팅 허니컴(honeycomb)프레임 3단 결합구조의 디자인으로 제안했다. 광촉매 필터의 살균소독 효과에 대해서는 향후 연구가 필요하며, 본 연구에서는 공기청정기에 광촉매 필터 적용 및 구조에 대하여 보고하고자 한다.