• Mass Spectrometry Letters
• /
• 제4권2호
• /
• pp.25-29
• /
• 2013

Proteomics for biomarker validation needs high throughput instrumentation to analyze huge set of clinical samples for quantitative and reproducible analysis at a minimum time without manual experimental errors. Sample preparation, a vital step in proteomics plays a major role in identification and quantification of proteins from biological samples. Tryptic digestion a major check point in sample preparation for mass spectrometry based proteomics needs to be more accurate with rapid processing time. The present study focuses on establishing a high throughput automated online system for proteolytic digestion and desalting of proteins from biological samples quantitatively and qualitatively in a reproducible manner. The present study compares online protein digestion and desalting of BSA with conventional off-line (in-solution) method and validated for real time sample for reproducibility. Proteins were identified using SEQUEST data base search engine and the data were quantified using IDEALQ software. The present study shows that the online system capable of handling high throughput samples in 96 well formats carries out protein digestion and peptide desalting efficiently in a reproducible and quantitative manner. Label free quantification showed clear increase of peptide quantities with increase in concentration with much linearity compared to off line method. Hence we would like to suggest that inclusion of this online system in proteomic pipeline will be effective in quantification of proteins in comparative proteomics were the quantification is really very crucial.

• 한국정보처리학회:학술대회논문집
• /
• 한국정보처리학회 2020년도 춘계학술발표대회
• /
• pp.231-234
• /
• 2020

In the past few years, the industrial internet of things (IIoT) has received great attention in various industrial sectors which have potentially increased a high level of integrity, availability, and scalability. The increasing of IIoT is expected to create new smart industrial enterprises and build the next generation smart system. However existing IIoT systems rely on centralized servers that are vulnerable to a single point of failure and malicious attack, which exposes the data to security risks and storage. To address the above issues, blockchain is widely considered as a promising solution, which can build a secure and efficient environment for data storing, processing and sharing in IIoT. In this paper, we propose a decentralized, peer-to-peer platform for secure data storing in industrial IoT base on the ethereum blockchain. We exploit ethereum to ensure data security and reliability when smart devices store the data.

• 대한안전경영과학회:학술대회논문집
• /
• 대한안전경영과학회 2012년 춘계학술대회
• /
• pp.43-59
• /
• 2012

As contemporary building construction type is getting higher and deeper,construction equipment usage is getting more, and related fatal accidents are on an increasing trend. In these days, due to the deteriorating construction business circumstance, job finding problem of equipment operator, and dumping contract, equipment lease suppliers are putin jeopardy. In high-rise building construction, especially tower crane, mobile elevated work platform and other High place operation cars among construction equipment cause many critical accidents because of drop supply of construction biding bringing out dumping contract could cause unsafe and poor construction management. Because of this, a method was drawn which could grasp the present state of construction equipment management and manage safety of the equipment more easily for accident prevention by choosing 2 kinds of Construction equipment which cause safety accident frequently among the equipment mainly used in construction site. This study suggested a method about construction equipment safety management using "smart phone" base which could be used in safety management for construction equipment by whomever in construction site. After attachment of QR code included safety checklist, It became possible that site managers could check more efficiently by scanning with their smart phone when they inspect equipment. Moreover, by the construction interested who didn't know what and how they have to inspect could point out unsafe condition in the early stage of equipment entering or take unsafe one out of the site by using new smart phone safety checking system is installed, it became possible that critical accident caused by construction equipment was prevented in advance.

• 한국ITS학회 논문지
• /
• 제7권5호
• /
• pp.1-12
• /
• 2008

본 논문에서는 고속으로 이동하는 차량에 실시간 교통정보서비스를 연속적으로 제공하기 위하여 C&R 서비스에 대한 개념 및 서비스 시나리오를 제시하고, seamless 서비스 구현에 필요한 정보통신 요소기술들과 C&R 통신시스템의 구조와 제공방법에 대해서 기술하였다 향후, 스마트 하이웨이 서비스는 160km/h 이강 고속으로 이동하는 차량에 실시간 C&R 서비스가 이루어지는 정보통신 환경을 구축하는 개념이라 할 수 있다. 지금까지 DSRC 시스템을 기반하의 환경에서는 저속 차량과 통신이 가능하였으나, 고속 이동 환경에는 한계가 있다. 따라서 본 논문에서는 이러한 문제점을 해결하기 위해서 DSRC 기지국과 다수의 중계기의 핸드오버 기술을 이용함으로써, 고속이동 환경에도 적용 가능하며 스마트 하이웨이 환경에 대응할 수 있는 구조를 제안하였다.

• Genomics & Informatics
• /
• 제17권1호
• /
• pp.5.1-5.9
• /
• 2019

The chinstrap (Pygoscelis antarcticus) and gentoo (P. papua) penguins are distributed throughout Antarctica and the sub-Antarctic islands. In this study, high-quality de novo assemblies of blood transcriptomes from these penguins were generated using the Illumina MiSeq platform. A total of 22.2 and 21.8 raw reads were obtained from chinstrap and gentoo penguins, respectively. These reads were assembled using the Oases assembly platform and resulted in 26,036 and 21,854 contigs with N50 values of 929 and 933 base pairs, respectively. Functional gene annotations through pathway analyses of the Gene Ontology, EuKaryotic Orthologous Groups, and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were performed for each blood transcriptome, resulting in a similar compositional order between the two transcriptomes. Ortholog comparisons with previously published transcriptomes from the $Ad{\acute{e}}lie$ (P. adeliae) and emperor (Aptenodytes forsteri) penguins revealed that a high proportion of the four penguins' transcriptomes had significant sequence homology. Because blood and tissues of penguins have been used to monitor pollution in Antarctica, immune parameters in blood could be important indicators for understanding the health status of penguins and other Antarctic animals. In the blood transcriptomes, KEGG analyses detected many essential genes involved in the major innate immunity pathways, which are key metabolic pathways for maintaining homeostasis against exogenous infections or toxins. Blood transcriptome studies such as this may be useful for checking the immune and health status of penguins without sacrifice.

• International Journal of Internet, Broadcasting and Communication
• /
• 제13권4호
• /
• pp.112-120
• /
• 2021

Recently, the number of consumers using digital online distribution platforms is increasing. This caused the rapid growth of the e-commerce market and increased delivery volume in urban areas. The logistics system, designed ar006Fund the city center to handle the delivery volume, operates a delivery system from the outskirts of the city to the urban area using cargo trucks. This maintains an ecosystem of high-cost and inefficient structures that increase social costs such as road traffic congestion and environmental problems. To solve this problem, research is being conducted worldwide to establish a high-efficiency urban joint logistics system using urban railway facilities and underground space infrastructure existing in existing cities. The joint logistics system begins with linking unmanned delivery automation services that link terminal delivery such as cargo classification and stacking, infrastructure construction that performs cargo transfer function by separating from passengers such as using cargo platform. To this end, it is necessary to apply the device to the vertical and horizontal transportation machine supporting the vertical transfer in the flat space of the joint logistics terminal, which is the base technology for transporting cargo using the transfer robot to the destination designated as a freight-only urban railway vehicle. Therefore, this paper aims to derive holistic viewpoints needs for design requirements for vertical and vertical transportation machines and freight transportation standard containers, which are underground railway logistics transport devices to be constructed by urban logistics ecosystem changes.

• 한국과학예술포럼
• /
• 제25권
• /
• pp.103-118
• /
• 2016

ICT의 비약적인 발전으로 기업 비즈니스 모델이 빠르게 변화했으며 더불어 급진적인 IT기술의 성장으로 순차적 혹은 점진적인 생존이 어렵게 되었다. IT서비스와 같이 인터넷을 기반으로 둔 새로운 사업들은 기존에 존재하지 않는 융·복합적인 새로운 비즈니스 모델을 찾으며 더욱 경쟁력 있는 비즈니스 모델을 만들어 내고자 고민하고 있지만, 과거에 성공했던 비즈니스 모델은 그 경제적인 효율성에 소멸되고 있다. 그러나 인터넷을 통한 플랫폼의 경우 플랫폼의 가치가 급격히 높아지는 임계점 도달이 기존에 비해 빠른 속도로 일어나게 되었기 때문에 모든 비즈니스에 있어서 비즈니스의 급격한 팽창을 위한 플랫폼화가 매우 중요한 조건이 되었다. 본 연구에서는 IT서비스 기획에 있어서 플랫폼 비즈니스 모델 수립의 필요성을 분석하고, 플랫폼 비즈니스 모델 사례들을 분석함으로써 플랫폼 비즈니스 모델의 특징을 도출하였다. 연구결과, 도출된 특징들은 첫째, 충분한 구매자와 판매자를 확보해야하고 둘째, 고객들에게는 해당 플랫폼만의 차별적인 가치가 창출되어 제공되어야 하며 셋째, 플랫폼 주도 기업과 참여자, 또는 참여자 간 공동의 이익이 존재해야 한다. 그리고 넷째로, 참여자의 기반 확대와 업그레이드, 인접 분야 확장 등으로 지속적인 확장성을 가지고 있어야 하며, 진화가 지속되어야 한다는 것이다. 도출된 특징들은 플랫폼 비즈니스 모델 수립과 서비스기획의 도식화에 많은 영향력을 미칠 것을 기대하며, 본 연구를 시작으로 연구에 언급되지 않은 플랫폼 비즈니스 수익모델의 이론이 정립되어 플랫폼 기반 IT서비스 기획을 하는 기획자들이 기획 초안 다지기에 있어서 보다 더욱 시간을 줄이고 큰 그림을 그려갈 수 있는 길이 될 수 있길 기대해본다.

• 한국정보시스템학회지:정보시스템연구
• /
• 제29권2호
• /
• pp.131-147
• /
• 2020

Purpose The purpose of this study is to develop intelligent vehicle parking distribution system based on LoRa network at the circumstance of traffic congestion during cultural festival in a local city. This paper proposes a parking dispatch and distribution system using a Q-learning algorithm to rapidly disperse traffics that increases suddenly because of in-bound traffics from the outside of a city in the real-time base as well as to increase parking probability in a parking lot which is widely located in a city. Design/methodology/approach The system get information on realtime-base from the sensor network of IoT (LoRa network). It will contribute to solve the sudden increase in traffic and parking bottlenecks during local cultural festival. We applied the simulation system with Queuing model to the Yudeung Festival in Jinju, Korea. We proposed a Q-learning algorithm that could change the learning policy by setting the acceptability value of each parking lot as a threshold from the Jinju highway IC (Interchange) to the 7 parking lots. LoRa Network platform supports to browse parking resource information to each vehicle in realtime. The system updates Q-table periodically using Q-learning algorithm as soon as get information from parking lots. The Queuing Theory with Poisson arrival distribution is used to get probability distribution function. The Dijkstra algorithm is used to find the shortest distance. Findings This paper suggest a simulation test to verify the efficiency of Q-learning algorithm at the circumstance of high traffic jam in a city during local festival. As a result of the simulation, the proposed algorithm performed well even when each parking lot was somewhat saturated. When an intelligent learning system such as an O-learning algorithm is applied, it is possible to more effectively distribute the vehicle to a lot with a high parking probability when the vehicle inflow from the outside rapidly increases at a specific time, such as a local city cultural festival.

• 한국정밀공학회지
• /
• 제27권12호
• /
• pp.99-106
• /
• 2010

Gas turbines operation for power generation increased rapidly since 1990 due to the high efficiency in combined cycle, relatively low construction cost and low emission. But the operation and maintenance cost for gas turbine is high because the expensive superalloy hot gas path parts should be repaired and replaced periodically This study analyzed the initiation and propagation of the crack at the gas turbine blades which are coated with MCrAIY as a bond coat and TBC as a top coat. The sample blades had been serviced at the actual gas turbines for power generation. Total 7 sets of blades were analyzed and they have different EOH(equivalent operation hour). Blades were sectioned and the cracking distribution were measured and analyzed utilizing SEM(scanning electron microscope) and optical microscope. The blades which had 52,000 EOH of operation had cracks at the substrate and the maximum depth was 0.2 mm. Most of the cracks initiated at the boundary layer between TBC and bond coat and propagated down to the bond coat. Once bond coat is cracked, the base metal is exposed to the oxidation condition and undergoes notch effect. Under this environment, the crack branched at the inter-diffusion layer and propagated to the substrate. Critical cracks affecting the blade life were analyzed as those on suction side and platform.

• 한국방사성폐기물학회:학술대회논문집
• /
• 한국방사성폐기물학회 2005년도 Proceedings of The 6th korea-china joint workshop on nuclear waste management
• /
• pp.76-85
• /
• 2005

The conceptual study of Accelerator Driven System (ADS) had lasted for about five years and ended in 1999 in China. As one project of 'the major state basic research program (973)' in energy domain, which is sponsored by the China Ministry of Science and Technology (MOST), a five years program of basic research for ADS physics and related technology has been launched since 2000 and passed national review last month. CIAE (China Institute of Atomic Energy), IHEP (Institute of High Energy Physics), PKU-IHIP (Institute of Heavy Ion Physics in Peking University) and other institutions are jointly carrying on the research. The research activities are focused on HPPA physics and technology, reactor physics of external source driven sub-critical assembly, nuclear data base and material study. For HPPA, a high current injector consisting of an ECR ion source, LEBT and a RFQ accelerating structure of 3.5MeV has been built. In reactor physics study, a series of neutron multiplication experimental study has been carried out and is being carrying on. The VENUS facility has been constructed as the basic experimental platform for the neutronics study in ADS blanket. It's a zero power sub-critical neutron multiplying assembly driven by external neutron produced by a pulsed neutron generator. The theoretical, experimental and simulation study on nuclear data, material properties and nuclear fuel circulation related to ADS is carrying on to provide the database for ADS system analysis. The main results on ADS related researches will be reported.