• 한방재활의학과학회지
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• 제31권1호
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• pp.81-93
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• 2021

Objectives The objective of the study was to investigate effects of ChondroT by improvement of blood metabolites in high-fat diet (HFD)-induced hyperlipidemia rat model. Methods Sprague-Dawley rats were randomly assigned to intact, control, simvastatin, and CT100, CT200 and CT400 (each n=6). For observing cholesterol change, animals were first fed high fat diet for 5 weeks and then high fat diet and drugs for 3 weeks. At the end of the experiment, total cholesterol, triglyceride, high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) were analyzed by obtained blood collection. Further, amplified leptin, peroxisome proliferator activated receptor (PPAR) and adiponectin DNA were observed by reverse transcription polymerase chain reaction analysis. Results Observing the effect of ChondroT on the change of lipid metabolism in hyperlipidemia-induced rats, triglyceride and total cholesterol were significantly decreased in SV100 group, HDL-C was significantly increased in SV100, CT100 and CT200 groups, and LDL-C was significantly decreased in SV100, CT100, CT200 and CT400 groups, compared to the control group. Leptin level in hyperlipidemia-induced rats was significantly decreased in CT100 and CT200 groups, compared to the control group. The effect of ChondroT on adiponectin level in hyperlipidemia-induced rats was significantly increased in SV100, CT100 and CT200 groups. PPAR level in hyperlipidemia-induced rats was significantly decreased in SV100, CT200 and CT400 groups. Platelete activating factor level in hyperlipidemia-induced rats was significantly decreased in CT100 and CT200 groups. Conclusions Based on these results, it could be suggested that ChondroT has certain effects of improving blood metabolites in HFD-induced hyperlipidemia.

• Biomolecules & Therapeutics
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• 제9권2호
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• pp.104-111
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• 2001

Gamigwaruhaebaekwhanggum-Tang (GGHWT) have been evaluated for antihyperlipidemic effects on experimental hyperlipidemic rats and mice induced by Triton WR-1339, com oil and high cholersterol-diet. Especially, GGHWT is formulated with Trichosanthis Fructus, Pinelliae Tuber, Aurantii Immaturus Fructus, Magnoliae Cortex, Allii Macrostemi Bulbuls, Cinnamomi Ramulus and Scutellariae Radix. Oral administration of GGHWT at 500 mg/kg/day for 3 days significantly inhibited the increase of serum triglyceride and LDL-cholesterol, liver triglyceride in hyperlipidemic rats induced by Triton WR-1339. And, GGHWT significantly inhibited the increase of serum triglyceride in hyperlipidemic rats induced by corn oil. Also, administration of GGHWT (500 mg/kg, once daily for 1 week, p.o.) prevented the increase of serum total cholesterol, triglyceride, LDL-cholesterol, liver total cholesterol and triglyceride in 1% cholesterol-diet fed mice. These results suggest that GGHWT is effective for the treatment of hyperlipidemia.

• Journal of Nutrition and Health
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• 제30권6호
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• pp.614-621
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• 1997

The experiments were performed to investigate the effects of protein and protein hydrolysates on lipid metabolism in the hyperlipidemic/hypercholesterolemic rat model induced by feeding fat-enriched diet. In Except 1 male rats were fed four semi-purified high fat and cholesterol diets that contained different nitrogen source, casein(C), casein hydrolysate(CH), corn gluten(G) and corn gluten hydrolysate(GH), for 6 weeks. In Expt. 2 rats were fed high fat and cholesterol diet for 4 weeks to induce hyperlipidemia and hypercholesterolemia. Then the rats were divided into 4 groups and were fed the four kinds of above experimental diets for 4 weeks consecutively. The contents of total lipid , cholesterol and triglyceride in blood, liver and feces were determined. Serum lipid concentrations of CH, G and GH were significantly lower than that of C. Serum cholesterol concentrations of hydrolysate groups(CH and GH) were significantly lower than those of intact protein groups(C and G). Serum HDL -cholesterol concentration tended to increase by hydrolysate intake. The total lipid, cholesterol contents in liver showed similarity results as above. Fecal lipid excretions of CH, G, and GH groups were significantly higher than that of C group. These results indicate that hypolipidemic and /or hypocholesterolemic effect of corn gluten or protein hydrolysates were detected in the process of inducing hyperlipidemia by high-fat and cholesterol diet or after inducing hyperlipidemia.

• Journal of Nutrition and Health
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• 제30권2호
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• pp.187-194
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• 1997

The effects of diet induced hyperlipidemia on kidney function were studied in Sprague-Dawley rats fed high fat diet containing 20% beef tallow and high cholesterol diet containing 5% cholesterol for 8, 12, 16 weeks, respectively. The concentrations of serum total lipid, total cholesterol and LDL-cholesterol were significantly high cholesterol diet groups during all experimental periods (p<0.05). HDL-cholesterol concentration was the lowest value in high cholesterol diet group of 16 weeks(p<0.05). Triglyceride concentration was not affected by experimental diets. Serum total protein, albumin and creatinine concentration tended to higher in high cholesterol diet groups than those in high fat diet groups. And serum urea-N concentration was higher in high fat diet group of 16 weeks than that in other diet groups. Urinary total protein and urea-N were higher in high cholesterol diet groups than those in other diet groups regardless of experimental period period. There was no significant difference in urinary creatinine concentratin among diet groups(p<0.05). GFR was lower in high cholesterol diet groups than that in high fat diet groups at 8, 16 weeks, respectively. Wet weight per body weight, total lipid, triglyceride, total cholesterol concentations of liver tissue were apparently high in high cholesterol diet groups(p<0.05). Kidney wet weight per body weight wer not affected by experimently diets, total lipid concentration of kidney tissue was significantly high in high fat diet groups of 12 weeks(p<0.05), kidney tissue triglyceride concentrations of high cholesterol diet groups of 12, 16 weeks apparently low, and total cholesterol concentration of kidney tissue was higher in experimental diet groups than that of control groups at 12, 16 weeks(p<0.05). Fecal excretion, total lipid, triglyceride and cholesterol concentrations of feces were markedly high in all high cholesterol diet groups except high fat diet group of 16 weeks. The results of light microscopic examination indicated that glomerulosclerosis was not obsrved in rats fed experimental diets.

• Biomolecules & Therapeutics
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• 제5권1호
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• pp.43-47
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• 1997

Effects of Bupleuri Radix, Paeonie Radix or Uncariae Ramulus et rncus on experimental yperlipidemia were studied. Hyperlipidemia was induced on male Wistar rats by feeding them on high holesterol diet for one week, as previously described by the authors. Blood lipid profile was verified on these ats by measuring total cholesterol(TC), triglyceride(TG), high density lipoprotein cholesterol(HDL) and low ensity lipoprotein cholesterol(LDL). Then, the rats were followed by feeding normal diet. At the same time, ethanol extracts of the three individual herb medicines were orally administered to the rats for 4 weeks and the parameters above mentioned were monitored. Methanol extract of 5Bupleuri radix reduced the TC value significantly at 2 week point and Paeoneae Radix reduced the TC value at 4 week point in compare to control group, suggesting the antihyperlipidemic effect of the two herbal medicines in vivo. The extract of Uncariae Ramulus et Uncus however did not show antihyperlipidemic effect in our experiment.

• 대한본초학회지
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• 제33권5호
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• pp.89-103
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• 2018

Objectives : This study is aimed to compare the changes in Antioxidative capacity of Liriopis Tuber by steaming process and to compare the effects in hyperlipidemia induced rats fed high cholesterol diet between Simvastatin and Liriopis Tuber by steaming process. Methods : The SD rats were divided into six groups: normal diet (Nor), high cholesterol diet (Veh), high cholesterol diet plus Simvastatin 5 mg/kg (Sim), high cholesterol diet plus LT0 extract 200 mg/kg (LT0), high cholesterol diet plus LT6 extract 200 mg/kg (LT6) and high cholesterol diet plus LT9 extract 200 mg/kg (LT9). We compared the total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL), low density lipoprotein cholesterol (LDL) contents and reactive oxygen species (ROS) from each serums. Protein expression in liver tissues related to antioxidant and cholesterol was analyzed. Results : The Antioxidant activity of Liriopis Tuber increased by steaming process. In vivo, TC, TG, LDL-c, atherogenic index (AI) and cardiac risk factor (CRF) decreased and HDL-c increased with increasing steaming frequency. aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine, and blood urea nitrogen (BUN) decreased with increasing steaming frequency. ROS decreased only in LT9, and SOD, catalase and glutathione peroxidase (GPx) increased with increasing steaming frequency. phospho-AMP-activated protein kinase (p-AMPK) increased and sterol regulatory element-binding protein 2 (SREBP-2), Phospho-Acetyl-CoA Carboxylase (p-ACC) and HMG-CoA reductase (HMGCR) decreased with increasing steaming frequency. Liver staining showed a decrease in hepatic fat accumulation of LT9. LT9 showed significant results in all experiments. Conclusions : LT9 showed significance of anti-lipid effect and improved fatty liver of hyperlipemia induced rats fed on high cholesterol diet, In conclusion, LP9 can be effectively used for the treatment of hyperlipidemia.

• 동의생리병리학회지
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• 제17권4호
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• pp.1092-1096
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• 2003

This study was carried out to investigate the clinical effects of Tongbiyeum(TBY) ₂on hyperlipidemia. The hyperlipidemia of rats was induced by feeding high cholesterol diet for 4 weeks. We checked serum lipids and body weight weekly for 4 weeks. After the termination of treatment, we measured liver weight and observed histopathological change. We compared result of TBY group with control group. Thereafter, we made a study of 37 hyperlipidemic patients who visited us, the oriental medical center Daejeon and cheongju of Daejeon University, from November, 2001 to April, 2002. After administering TBY for 1 month, we followed up their total cholesterol, triglyceride and HDL-Cholesterol levels. The results were as follows; TBY treatment inhibited the liver weight gain induced by high cholesterol diet as compared with control group. TBY treatment inhibited lipid droplet accumulation and apoptotic change in liver as compared with control group. TBY treatment significantly inhibited the increasing of serum triglyceride and total cholesterol levels induced by high cholesterol diet as compared with control group but not affect HDL-cholesterol level. After administering TBY, the serum total cholesterol, triglyceride and LDL-cholesterol levels of hyperlipidemic patients were decreased significantly. After administering TBY, the serum HDL-Cholesterol level of hyperlipidemic patients had no significant changes. According to above mentioned results, we can infer that TBY has hypolipidemic effect to be applicable to artheriosclerosis.

• 동의생리병리학회지
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• 제18권2호
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• pp.431-435
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• 2004

The purpose of this study was to examine the effects of Salviae Miltiorrhizae Radix on the high fat diet-induced hyperlipidemia in rats. Sixty male Sprague-Dawley rats, around 270g of B.W., were divided into normal, control, sample A and sample B group. Normal and control groups were orally administrated with saline during 4 weeks, sample A and sample B with 252.5mg/kg(rat) and 420.1 mg/kg(rat) of solid extract of Salviae Miltiorrhizae Radix during 4 weeks respectively. Blood samples were collected from each of animals at 4 weeks, and used for the blood chemical analysis. Animals were sacrificed at 4 weeks, and chemical analysis was performed on the collected liver samples. The serum total cholesterol, HDL-cholesterol, LDL-cholesterol levels of sample A group were significantly decreased compared with the control group. On the basis of these of results, it is confirmed that Salviae Miltiorrhizae Radix has the curative effects on the hyperlipidemia in rats induced by high fat diet

• 동의생리병리학회지
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• 제19권2호
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• pp.495-500
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• 2005

To compare the efficacy of Stephaniae Tetrandrae Rhizoma and Sinomenii Caulis which are medicinal plants used as Bang Kee(防己), the plants were combined with Astragali Radix and Lycopii Herba into 6 different medicinal decoctions. The specimen decoctions prepared were each decoctions of Stephaniae Tetrandrae Rhizoma(St), Sinomenii Caulis(Si), Stephaniae Tetrandrae Rhizoma-Astragali Radix(StA), Stephaniae Tetrandrae Rhizoma-Lycopii Herba(StL), Sinomenii Caulis-Astragali Radix(SiA) and Sinomenii Caulis-Lycopii Herba(SiL). They were dosed to rats with induced hyperlipidemia. In the groups of rats with hyperlipidemia induced by high cholesterol diet, in the group which St was dosed to, there was no significant effect on the level of Tc, TG, HDLc. And in the group which Si was dosed to, the level of Tc, TG dropped and that of HDLc rose. In the groups of rats with hyperlipidemia induced by high cholesterol diet, in the group which StA was dosed to, there was no significant change in the level of Tc, TG, or HDLc. But in the group which SiA was dosed to, the level of Tc and TG dropped and that of HDLc rose. In the groups of rats with hyperlipidemia induced by high cholesterol diet, in the group which StL was dosed to, the level of TG dropped, but there was no significant change in the level of Tc or HDLc. And in the SiL group, there was no significant change in the level of Tc or TG, but the level of HDLc rose. On diseases such as hyperlipidemia, when using drug couple of Bang Kee-Astragali Radix as a treatment, Sinomenii Caulis is recommended rather than Stephaniae Tetrandrae Rhizoma, and when using drug couple of Bang Kee-Lycopii Herba as a treatment, Sinomenii Caulis and Stephaniae Tetrandrae Rhizoma neither showed significant effect. And as Lycopii Herba interrupts the effect of Sinomenii Caulis, drug couple of Sinomenii Caulis-Lycopii Herba is not recommended.

• 대한한방내과학회지
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• 제31권3호
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• pp.526-538
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• 2010

Objective : This study was performed to investigate the effect of Sunhwangigagambang(SHG) on hyperlipidemia in SD rats induced by high cholesterol diet. Method : After treatment with SHG, cytotoxicity, body weight, liver weight, AST, ALT, ALP, total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, glucose, albumin, total protein in serum, malondialdehyde, and gene expression for ACAT and HMG-CoA reductase in hepatic tissue were analyzed. Result : 1. SHG didn't show any cytotoxicity in both human fibroblast cell line and SD rats. 2. SHG significantly inhibited the increase of liver weight by high cholesterol diet compared to the control group. 3. SHG significantly ameliorated the increase of total cholesterol, LDL-cholesterol, and triglyceride and reduction of HDL-cholesterol compared to the control group. 4. SHG significantly reduced glucose level in serum compared to the control group. 5. SHG significantly reduced malondialdehyde in hepatic tissue compared to the control group. 6. SHG significantly down-regulated gene expression of ACAT and HMG-CoA reductase compared to the control group. Conclusion : These results suggest that SHG might be effective in treatment and prevention of hyperlipidemia.