Proceedings of the Korean Society of Grassland Science Conference
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한국초지조사료학회 2002년도 창립 30주년 International Symposium
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pp.159-183
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2002
China is the country with more people and more land, faced with the pressure of population growth and land decrement. The potential of developing animal husbandry by increasing feed grain per capita is less and less. "Planting forage to feed livestock" is a sustainable industry with grain-saying, high efficiency, good quality and safety, accorded with the conditions of China. First, the natural, social and economical condition in southern area of China was analyzed, and the characteristics of production and utilization of forage were introduced in this paper. The natural condition in southern area of China was well suited for forage production. Forage was mainly planted on famland. Main herbage cultivars were annual or hibernal annual. Most of forage was directly utilized by herbivorous animal and fish. Second, The present situation, developmental trend and existing problems of forage industrialization were reviewed. The level of forage industrialization was low with a bright future. At present, the chief existing problems were lack of herbage cultivars of good quality, backward equipment, lagged techniques and low level of mechanization in forage processing with a short time and so on. Finally, the situation of production and utilization of forage was summarized.
Barley has nutritional benefits due to its high dietary fiber content; therefore, the intake of whole barley grains is recommended. However, barley is often consumed in the fermented form because of the improved texture and digestibility. The present study was designed to elucidate the intracellular signaling pathway for macrophage activation by the polysaccharide BF-CP from fermented barley. BF-CP is a neutral polysaccharide, composed of neutral sugars, including glucose (70.7%), xylose (11.4%), and arabinose (9.0%). BF-CP exhibited macrophage-stimulatory activity by inducing the production of interleukin (IL)-6, tumor necrosis factor $(TNF)-{\alpha}$, and nitric oxide in RAW 264.7 macrophages. Further, BF-CP treatment strongly increased the IL-6 and $TNF-{\alpha}$ gene expression in a concentration-dependent manner. Signal transduction experiments using immunoblotting showed that BF-CP phosphorylated mitogen-activated protein kinases (MAPKs), such as c-Jun N-terminal kinase, extracellular signal-regulated kinase, and p38, and nuclear factor $(NF)-{\kappa}B$, in RAW 264.7 cells in a concentration-dependent manner. These results suggest that BF-CP activates the macrophages via MAPK and $NF-{\kappa}B$ pathways, and also induces an increase in the production of cytokines.
The regulation of HNS has been intensified by the international trends including OPRC-HNS Protocol and '96 Convention which was driven by the high amounts of cargo. Due to the characteristics of HNS that would possibly bring potential damage with personnel and assets, effective management and prompt actions are required definitely. In order to respond effectively against HNS accidents, Korea Coast Guard (KCG) is in the process q development of HNS accident response manual and information system which allows On-Scene Coordinator(OSC) and personnels for rescue including an information for hazardous materials, sensitive area to be affected, solution methods and more. Furthermore, KCG is also building up establishment of national and local contingency plans for HNS in accordance with OPRC-HNS Protocol. It is also advised to proceed for the government to solve the anticipated obstacles that include protection equipments to get close to the site, experts allowing to manage accidents and organizations specialized for overall HNS related matters. The proposed issues described above are planned to conducted on basis of government.
Oh, Jong Min;Lee, Jae Pil;Baek, Seung Cheol;Jo, Yang Do;Kim, Hoon
Journal of Microbiology and Biotechnology
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제28권5호
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pp.757-764
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2018
A cellulolytic fungus, YDJ14, was isolated from compost and identified as an Aspergillus sp. strain. Three extracellular ${\beta}$-glucosidases, BGL-A1, BGL-A2, and BGL-A3, were separated using ultrafiltration, ammonium sulfate fractionation, and High-Q chromatography. The molecular masses of the three enzymes were estimated to be 100, 45, and 40 kDa, respectively, by SDS-PAGE. The optimum pH and temperature of BGL-A3 were 5.0 and $50^{\circ}C$, respectively, whereas the optimum pH and temperature of BGL-A1 and BGL-A2 were identical (4.0 and $60^{\circ}C$, respectively). The half-life of BGL-A3 at $70^{\circ}C$ (2.8 min) was shorter than that of BGL-A1 and BGL-A2 (12.1 and 8.8 min, respectively). All three enzymes preferred p-nitrophenyl-${\beta}$-$\text\tiny{D}$-glucopyranoside (pNPG) and hardly hydrolyzed cellobiose, suggesting that these enzymes were aryl ${\beta}$-glucosidases. The $K_m$ of BGL-A3 (1.26 mM) for pNPG was much higher than that of BGL-A1 and BGL-A2 (0.25 and 0.27 mM, respectively). These results suggested that BGL-A1 and BGL-A2 were similar in their enzymatic properties, whereas BGL-A3 differed from the two enzymes. When tilianin (a flavone glycoside of acacetin) was reacted with the three enzymes, the inhibitory activity for monoamine oxidase, a target in the treatment of neurological disorders, was similar to that shown by acacetin. We conclude that these enzymes may be useful in the hydrolysis of flavone glycosides to improve their inhibitory activities.
The fluctuations of sea surface temperatures (SST) and their anomalies in the Tsushima Current region are studied by means of empirical orthogonal function (EOF) analysis of the monthly SST data for 30 years (1941-1970) at 8 coastal stations. The overall features of the seasonal variation of SST are described by the first EOF mode, which explains $97.2\%$ of the total variance. Annual ranges of seasonal variation of SST and root-mean-square amplitudes of SST anomalies in the downstream of the Tsushima Current are larger than those in the upstream. The SST anomalies in the Tsushima Current region consist of simultaneous fluctuations, which explain $40.9\%$ of the total variance, and 'see-saw' fluctuations of which rise and fall in the upstream are opposite to those in the downstream. The latter second EOF mode explains $19.3\%$ of the total variance. We generated the low-pass (periods longer than 24 months), band-pass (periods between 6 and 24 months) and high-pass (periods shorter than 6 months) SST anomaly series and analyzed them by EOF method. The spatial distributions of the first and second EOF modes of all filtered SST anomalies are similar to each other.
Journal of the Korea Academia-Industrial cooperation Society
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제19권7호
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pp.174-182
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2018
Given the ongoing debate in many aspects of finance, more attention may need to focus on corporate R&D expenditures. This study empirically tests financial determinants of R&D expenditures for NYSE-listed and KOSPI-listed firms. Three major hypotheses were postulated to test for corporate R&D outlay. First, proposed variables such as one-year lagged R&D expenditures, market value based leverage, profitability and cash holdings showed significant influence on corporate R&D costs for the sample firms. Moreover, financial factors inclusive of squared one-year lagged R&D expenditures, the interaction effect between one-lagged R&D expenditures and high-growth firm, non-debt tax shield, Tobin's q and a dummy variable to explain differences in accounting treatment between the U.S. and Korea, revealed significant differences between the two samples. Finally, in the conditional quantile regression (CQR) analysis for the R&D-related variables in relation to corporate growth rate, it was found that the NYSE-listed firms had a statistically significant linkage between growth potential and one-year lagged R&D expenditures at lower quantile levels. This study may shed new light on identifying financial factors affecting differences between the U.S. market (as an advanced market) and the Korean market (as an emerging market) regarding the optimal level of R&D investments for shareholders.
This study was done for the purpose of analyzing the job-satisfaction and its depressive factors in human relation of operating room nurses of university hospital. Therfore, it makes an offer the basic data to help the resolution and prevention of the problems in operating room nurses. Furthermore, this study was conducted in order to find out some kinds of scientific data for the better control of depressive factors of job-satisfaction expressed by the operating room nurses. The structred questionaire reports of 246 operating room nurses who were employed in 5 different university hospitals which have over 1, 000 beds located in Seoul, Korea were used, which wer collected from August 24th to August 30th of 1992. The author visited supervisors of operating room in each university hospital and explained the aim of this study. The most of them(90.0%) answered to the questionaires. Analysis of the collected data were done by mean, standard deviation, percentage, t-test, F-test, Q-test, correlation analysis, one-way ANOVA.and stepwise multiple regression analysis. Major findings of this study were as follows: 1. The job of the operating room nurses were remarkably related with the satisfaction in human relation, which was defined as the behavioral job with thoughtful action rather than with mechanical action. However, the degree of satisfaction in human relation with personnel in other departments was found to be the lowest and its the main depressive factors were appeared due to the absence of interaction and uncooperative attitudes. Therefore, it was required that the members of other job need more cooperative attitude to the actual works in the operating room nurses. 2. The depressive factors in the satisfaction degree of human relation with offical seniors wer significantly related with their irresponsibility and partialness. Moreover, the job attitude of the operating room nurses is abundantly required to be improved, 3. The depressive factors in the satisfaction of human relation with medical doctors were significantly related with their insincerity and the class-consciousness. It is clearly suggested that the medical doctors do more cooperative behaviors for the jobs of the operating room without the suggested that the medical doctors do more cooperative behaviors for the jobs of the operating room without the superiority feeling of class-consciousness. 4. There was a significantly positive association between the satisfaction degree of human relation of the nurses with the following charateristics. (1) The registered nurses which had a tendency to be satisfied about the registered nurses' job. (2) The registered nurses which had a tendency to be a technique-aptitued in the motivation of the registered nurses'job. (3) The registered nurses which had a tendency to be satisfied about the total hospital working carrier and the aspects of old ages. Finally, these findings indicated that the operating room nurses should require the professional education courses associated with a high-development techniques and setisfaction in their jobs.
Survey was initiated on insect pest species damaging the landscaping trees in golf courses to get the basic information for the development of insect management program. Four country clubs were selected as survey sites; Tongue Country Club and Youngwon Country Club in Gyeongnam, Daegu Country Club in Gyeongbuk, and Dongrae Benest Golf Club in Busan. We found that 60 species of 44 genera in 34 families of six orders from 88 species of 54 genera in 33 families of 22 orders surveyed. Most landscaping trees were damaged by one or two species of insect pests, but tree species such as Robinia pseudoacacia, Prunus leveilleana var. pendula, Castanea crenata, Quercus dentata, and Q. variabilis were damaged by more than five species of insects. Most insect species damaged one or two tree species. However, Adoretus tenuimaculatus(Coleoptera: Scarabaeidae) damaged 67 tree species of 41 genera. The Coleoptera and Honmoptera species were 36.4% and 36.3% of the total insect species, respectively. Insect pest occurring in high frequency was 9% of total insect species, while infrequent one was 73%. The insect species with chewing and sucking mouth part types were 49% and 51%, respectively.
Objective: This study evaluated the effects of different levels of protein concentrate supplementation on the growth performance of yak calves, and correlated the growth rate to changes occurring in the plasma- amino acids, -insulin profile, and signaling activity of mammalian target of rapamycin (mTOR) cascade to characterize the mechanism through which the protein synthesis can be improved in early weaned yaks. Methods: For this study, 48 early (3 months old) weaned yak calves were selected, and assigned into four dietary treatments according to randomized complete block design. The four blocks were balanced for body weight and sex. The yaks were either grazed on natural pasture (control diet) in a single herd or the grazing yaks was supplemented with one of the three protein rich supplements containing low (17%; LP), medium (19%; MP), or high (21%; HP) levels of crude proteins for a period of 30 days. Results: Results showed that the average daily gain of calves increased (0.14 vs 0.23-0.26 kg; p<0.05) with protein concentrates supplementation. The concentration of plasma methionine increased (p<0.05; 8.6 vs $10.1-12.4{\mu}mol/L$), while those of serine and tyrosine did not change (p>0.05) when the grazing calves were supplemented with protein concentrates. Compared to control diet, the insulin level of calves increased (p<0.05; 1.86 vs $2.16-2.54{\mu}IU/mL$) with supplementation of protein concentrates. Addition of protein concentrates up-regulated (p<0.05) expression of mTOR-raptor, mammalian vacuolar protein sorting 34 homolog, the translational regulators eukaryotic translation initiation factor 4E binding protein 1, and S6 kinase 1 genes in both Longissimus dorsi and semitendinosus. In contrast, the expression of sequestosome 1 was down-regulated in the concentrate supplemented calves. Conclusion: Our results show that protein supplementation improves the growth performance of early weaned yak calves, and that plasma methionine and insulin concentrations were the key mediator for gene expression and protein deposition in the muscles.
Zhang, Chen;Zhou, Zhengfu;Zhang, Wei;Chen, Zhen;Song, Yuan;Lu, Wei;Lin, Min;Chen, Ming
Journal of Microbiology and Biotechnology
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제25권12호
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pp.2125-2134
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2015
IrrE is a highly conserved global regulator in the Deinococcus genus and contributes to survival from high doses of UV radiation, ionizing radiation, and desiccation. Drad-IrrE and Dgob-IrrE from Deinococcus radiodurans and Deinococcus gobiensis I-0 each share 66% sequence identity. However, Dgob-IrrE showed a stronger protection phenotype against UV radiation than Drad-IrrE in the D. radiodurans irrE-deletion mutant (ΔirrE), which may be due to amino acid residues differences around the DNA-binding HTH domain. Site-directed mutagenesis was used to generate a Drad-IrrE A184S single mutant, which has been characterized and compared with the ΔirrE mutant complemented strain with Drad-irrE, designated ΔirrE-E. The effects of the A184S mutation following UV radiation and mitomycin C (MMC) shock were determined. The A184S mutant displayed significantly increased resistance to UV radiation and MMC shock. The corresponding A184 site in Dgob-IrrE was inversely mutated, generating the S131A mutant, which exhibited a loss of resistance against UV radiation, MMC shock, and desiccation. qPCR analysis revealed that critical genes in the DNA repair system, such as recA, pprA, uvrA, and ddrB, were remarkably induced after UV radiation and MMC shock in the ΔirrE-IE and A184S mutants. These data suggested that A184S improves the ability against UV radiation and MMC shock, providing new insights into the modification of IrrE. We speculated that the serine residue may determine the efficiency of DNA binding, leading to the increased expression of IrrE-dependent genes important for protection against DNA damage.
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