• Journal of Korean Society of Forest Science
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• v.90 no.4
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• pp.535-542
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• 2001

The objective of this study is to develop a spatial analysis modeling technique to evaluate the functional suitability of forest lands for land slide prevention. The functional suitability is classified into 3 categories of high, medium and low according to the potential of land slide on forest lands. The potential of land slide hazards is estimated using the measurements of 7 major site factors : slope, bed rock, soil depth, shape of slope, forest type and D.B.H. class of trees. The analytic hierarchical process is applied to determining the relative weight of site factors in estimating the potential of land slides. The spatial analysis modeling starts building base layers for the 7 major site factors by $25m{\times}25m$ grid analysis or TIN analysis, reclassifies them and produces new layers containing standardized attribute values, needed in estimating land slide potential. To these attributes, applied is the weight for the corresponding site factor to build the suitability classification map by map algebra analysis. Then, finally, cell-grouping operations convert the suitability classification map to the land unit function map. The whole procedures of the spatial analysis modeling are presented in this paper.

• Animal Bioscience
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• v.34 no.6
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• pp.957-966
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• 2021

Objective: Ovarian follicular development, which dependent on the proliferation and differentiation of granulosa cells (GCs), is a complex biological process in which miRNA plays an important role. Our previous study showed that miR-458b-5p is associated with ovarian follicular development in chicken. The detailed function and molecular mechanism of miR-458b-5p in GCs is unclear. Methods: The luciferase reporter assay was used to verify the targeting relationship between miR-458b-5p and catenin beta-1 (CTNNB1), which is an important transcriptional regulatory factor of the Wnt/β-catenin pathway. The cell counting kit-8 (CCK-8) assay, flow cytometry with propidium iodide (PI) and annexin V-fluorescein isothiocyanate (FITC) labeling were applied to explore the effect of miR-458b-5p on proliferation, cell cycle and apoptosis of chicken GCs. Quantitative real-time polymerase chain reaction and Western blot were used to detect the mRNA and protein expression levels. Results: We demonstrated that the expression of miR-458b-5p and CTNNB1 showed the opposite relationship in GCs and theca cells of hierarchical follicles. The luciferase reporter assay confirmed that CTNNB1 is the direct target of miR-458b-5p. Using CCK-8 assay and flow cytometry with PI and Annexin V-FITC labeling, we observed that transfection with the miR-458b-5p mimics significantly reduced proliferation and has no effects on apoptosis of chicken GCs. In addition, miR-458b-5p decreased the mRNA and protein expression of CD44 molecule and matrix metallopeptidase 7, which are the downstream effectors of CTNNB1 in Wnt/β-Catenin pathway and play functional roles in cell proliferation. Conclusion: Taken together, the data indicate that miR-458b-5p regulates ovarian GCs proliferation through Wnt/β-catenin signaling pathway by targeting CTNNB1, suggesting that miR-458b-5p and its target gene CTNNB1 may potentially play a role in chicken ovarian follicular development.

• Journal of the Korean Society of International Agriculture
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• v.31 no.4
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• pp.378-383
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• 2019

To determine whether FT-IR spectral analysis based on multivariate analysis for whole cell extracts can be used to discriminate papaya at metabolic level. FT-IR spectral data from leaves were analyzed by principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA). FT-IR spectra confirmed typical spectral differences between the frequency regions of 1,700-1,500, 1,500-1,300 and 1,100-950 cm^-1, respectively. These spectral regions were reflecting the quantitative and qualitative variations of amide I, II from amino acids and proteins (1,700-1,500 cm^-1), phosphodiester groups from nucleic acid and phospholipid (1,500-1,300 cm^-1) and carbohydrate compounds (1,100-950 cm^-1). The result of PCA analysis showed that papaya leaves could be separated into clusters depending on different growth temperature. In this case, showed discrimination confirmed according to metabolite content of growth condition from papaya. And PLS-DA analysis also showed more clear discrimination pattern than PCA result. Furthermore, these metabolic discrimination systems could be applied for rapid selection and classification of useful papaya cultivars.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.13-13
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• 2015

Rice blast fungus, Magnaporthe oryzae, is the most destructive pathogen of rice in the world. This fungus has a biotrophic phase early in infection and switches to a necrotrophic lifestyle after host cell death. During the biotrophic phase, the fungus competes with host for nutrients and oxygen. Continuous uptake of oxygen is essential for successful establishment of blast disease of this pathogen. Here, we report transcriptional responses of the fungus to oxygen limitation. Transcriptome analysis using RNA-Seq identified 1,047 up-regulated genes in response to hypoxia. Those genes were involved in mycelial development, sterol biosynthesis, and metal ion transport based on hierarchical GO terms and well-conserved among three different fungal species. In addition, null mutants of three hypoxia-responsive genes were generated and tested for their roles on fungal development and pathogenicity. The mutants for a sterol regulatory element-binding protein gene, MoSRE1, and C4 methyl sterol oxidase gene, ERG25, exhibited increased sensitivity to hypoxia-mimetic agent, increased conidiation, and delayed invasive growth within host cells, suggesting important roles in fungal development. However, such defects did not cause any significant decrease in disease severity. The other null mutant for alcohol dehydrogenase gene, MoADH1, showed no defect in the hypoxia-mimic condition and fungal development. Taken together, this comprehensive transcriptional profiling in response to a hypoxia condition with experimental validations would provide new insights on fungal development and pathogenicity in plant pathogenic fungi.

• Journal of Environmental Science International
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• v.24 no.5
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• pp.623-631
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• 2015

Microarray technology provides a unique tool for the determination of gene expression at the level of messenger RNA (mRNA). This study, the mRNA expression profiles provide insight into the mechanism of action of cadmium in Fleshy shrimp (Fenneropenaeus chinensis). The ability of genomic technologies was contributed decisively to development of new molecular biomarkers and to the determination of new possible gene targets. Also, it can be approach for monitoring of trace metal using oligo-chip microarray-based in potential model marine user level organisms. 15K oligo-chip for F. chinensis that include mostly unique sets of genes from cDNA sequences was developed. A total of 13,971 spots (1,181 mRNAs up- regulated and 996 down regulated) were identified to be significantly expressed on microarray by hierarchical clustering of genes after exposure to cadmium for different conditions (Cd24-5000 and Cd48-1000). Most of the changes of mRNA expression were observed at the long time and low concentration exposure of Cd48-1000. But, gene ontology analysis (GO annotation) were no significant different between experiments groups. It was observed that mRNA expression of main genes involved in metabolism, cell component, molecular binding and catalytic function. It was suggested that cadmium inhibited metabolism and growth of F. chinensis.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.42 no.12
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• pp.61-70
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• 2005

In this paper, we propose an efficient global placement algorithm which is an enhanced version of Hybrid Placer$^{[25]}$, a standard cell placement tool, which uses a middle-down approach. Combining techniques used in the well-known partitioner hMETIS and the RBLS(Relaxation Based Local Search) in Hybrid Placer improves the quality of global placements. Partitioning techniques of hMETIS is applied in a top-down manner and RBLS is used in each level of the top-down hierarchy to improve the global placement. The proposed new approach resolves the problem that Hybrid Placer seriously depends on initial placements and it speeds up without deteriorating the placement quality. Experimental results prove that solutions generated by the proposed method on the MCNC benchmarks are comparable to those by FengShui which is a well known placement tool. Compared to the results of the original Hybrid Placer, new method is 5 times faster on average and shows improvement on bigger circuits.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.11 no.2
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• pp.89-94
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• 2011

Today, the utilization of communication devices is being increased including information terminals, cell phones, handheld personal digital assistants (PDA) caused by the development of information and communication technology. The development of information and services is speeding up, whereas most communication devices have provided a inefficient hierarchical menu and sequential searching structure. In this study, the Adaptive User Interface is applied to the Bus Information Terminal(BIT) which is one of communication equipment installed in the bus stop. It will be based on analysis of unspecified individuals' preferences and user's directly personalization in the BIT prototype. We expect the results of this study to be possible to provide users with efficient and convenient information acquisition and contribute to the development of public transport use by improving the accessibility and usability of BIT.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.7
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• pp.1062-1071
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• 2018

The misuse of anabolic hormones or illegal drugs is a ubiquitous problem in animal husbandry and in food safety. The ban on growth promotants in food producing animals in the European Union is well controlled. However, application regimens that are difficult to detect persist, including newly designed anabolic drugs and complex hormone cocktails. Therefore identification of molecular endogenous biomarkers which are based on the physiological response after the illicit treatment has become a focus of detection methods. The analysis of the 'transcriptome' has been shown to have promise to discover the misuse of anabolic drugs, by indirect detection of their pharmacological action in organs or selected tissues. Various studies have measured gene expression changes after illegal drug or hormone application. So-called transcriptomic biomarkers were quantified at the mRNA and/or microRNA level by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) technology or by more modern 'omics' and high throughput technologies including RNA-sequencing (RNA-Seq). With the addition of advanced bioinformatical approaches such as hierarchical clustering analysis or dynamic principal components analysis, a valid 'biomarker signature' can be established to discriminate between treated and untreated individuals. It has been shown in numerous animal and cell culture studies, that identification of treated animals is possible via our transcriptional biomarker approach. The high throughput sequencing approach is also capable of discovering new biomarker candidates and, in combination with quantitative RT-qPCR, validation and confirmation of biomarkers has been possible. These results from animal production and food safety studies demonstrate that analysis of the transcriptome has high potential as a new screening method using transcriptional 'biomarker signatures' based on the physiological response triggered by illegal substances.

• Journal of Korean Academy of Nursing Administration
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• v.21 no.3
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• pp.277-286
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• 2015

Purpose: This study was done to identify the status of smartphone addiction among nursing students and to examine the mediating effects of Internet ethics on the relationship between smartphone addiction, physical symptoms and psychological well-being. Methods: Data were collected between May 20 and June 23, 2014. Participants were a convenience sample of 214 nursing students of one university in Seoul. Structured questionnaires were used to measure degree of smartphone addiction, physical symptoms, psychological well-being and Internet ethics. Data was analyzed using Independent t-test, One-way ANOVA, Pearson correlation coefficient, multiple hierarchical regression. Results: Of the nursing students, 14.5% had high level of risk of smartphone addiction and 16.8% had potential risk. Higher frequency of physical symptoms and lower level of Internet ethics were related to the higher level of smartphone addiction. Voluntary-control in Internet ethics had mediating effects on the relationship between smartphone addiction and physical symptoms. Conclusion: The results suggest that the level of smartphone addiction may lowered in nursing students by intensifying Internet ethics especially voluntary-control. Based on these findings, Internet ethics program for nursing students should be developed to facilitate the effective use of smartphones.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.16 no.1
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• pp.46-56
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• 1991

In this paper, 16word X 8bit Content Addressable and Reentrant Memory(CARM) is described. This device has 4 operation modes(read, write, match, reentrant). The read and write operation of CARM is like that of static RAM, CARM has the reentrant mode operation where the on chip garbage collection is accomplished conditionally. Thus function can be used for high speed matching unit of dynamic data flow computer. And CARM also can encode matching address sequentially according to therir priority. CARM consists of 8 blocks(CAM cell, Sequential Address Encoder(S.A.E). Reentrant operation. Read/Write control circuit, Data/Mask Register, Sense Amplifier, Encoder. Decoder). Designed DARM can be used in data flow computer, pattern, inspection, table look-up, image processing. The simulation is performed using the QUICKSIM logic simulator and Pspice circuit simulator. Having hierarchical structure, the layout was done using the 3{\;}\mu\textrm{m} n well CMOS technology of the ETRI design rule.