• 한방안이비인후피부과학회지
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• 제19권1호
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• pp.79-92
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• 2006

This study was carried out to evaluate antioxidative, cytotoxic and chemosensitizing effects for anti-tumor activities of Naesosungma-tang (NST). The results were as follows ; 1. The oxidative effects were measured by DPPH radical scavenging activity. NST water extract was showed more effective than ethanol extract and also various solvent fractions from NST showed effective in the following order : butanol fraction > ethyl acetate fraction > hexane fraction > aqueous fraction. 2. NST water extract was showed cytotoxic effects on the human cancer cells Calu-6 cell and SNU-601 cell, but not on MCF-7 cell. 3. The viability of cells was measured in 4 kinds of solvent fractions from water extract of NST by MTT assay. The results were as followings : 1) On the NIH3T3 cell, $IC_{50}$ value was $200{\sim}300\;{\mu}g/m{\ell}$ in three fractions except aqueous fraction. 2) On the Caco-2 cell, effective only in the butanol fraction. 3) On the Calu-6 cell, most effective in hexane fraction($IC_{50}:80\;{\mu}g/m{\ell}$) and effective in ethyl acetate and butanol fraction($IC_{50}:100{\sim}120\;{\mu}g/m{\ell}$). 4) On the MCF-7 cell, $IC_{50}$ value was similar in three fractions except aqueous fraction. 5) On the SNU-601 cell, $IC_{50}$ value was within $100\;{\mu}g/m{\ell}$ in three fractions except aqueous fraction. 6) On the HCT-1l6 cell, $IC_{50}$ value was $150{\sim}200\;{\mu}g/m{\ell}$ in three fractions except aqueous fraction. 7) On the AML-2/WT cell, not effective in four kinds of solvent fractions. 4. In combined effects of NST and vincristine on AML-2/D100 cell, water and ethanol extracts of NST and hexane fraction of water extracts of NST more effectively inhibited proliferation of AML-2/D100 cell. These results Suggest that NST has antioxidative and cytotoxic effects against Caco-2, Calu-6, MCF-7, SNU-601, HCT116 and NIH3T3 cell especially Calu-6 and SNU-601 cell, and also chemosensitizing effects against AML-2/D100 cell in combined vincristine.

• 생명과학회지
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• 제9권3호
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• pp.248-252
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• 1999

Effects of the ultrasonic treatment on plant represents antifeeding activity and chitin synthesis inhibition activity against diamondback moth (Plutella xylostella L.) by a leaf-dipping method at a concentration of 5,000ppm. Methanol extracts of leaves of the ultrasonic treatment on plant was sequentially partitioned with n-hexane, chloroform, ethlyacetate, butanol and water fractions, and bioassayed. Antifeeding activities were observed in n-hexane, chloroform and ethlyacetate fractions. N-hexane fraction has 94.6% of strongest antifeeding activity against P. xylostella. From the n-hexane sequentially partitioned showed H6 and H8 fractions of antifeeding activities against P. xylostella. And chitin synthesis inhibition activity against P. xylostella was obtained the methaol, n-hexane and water fractions.

• 한국균학회지
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• 제45권3호
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• pp.175-187
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• 2017

Laetiporus sulphrueus var. miniatus is widely distributed worldwide, and has commonly been used as a medicinal mushroom. In the present study, we investigated the effects of water extract and solvent fractions from the Laetiporus miniatus as possible antioxidant, anti-thrombin and anti-invasive agents against phorbol 12-myristate 13-acetate (PMA)- or thrombin-induced matrix metalloproteinase-2 (MMP-2) and MMP-9 activities. Samples were fractionated into n-hexane, $CHCl_3$, ethyl acetate, n-butanol, and water fractions, and individually analysed. The water fraction had the highest extraction yield at 34.90% (w/w), while the n-butanol fraction demonstrated the highest anti-oxidative activity at 81.44%. In the thrombin inhibitory activity test, the water fraction exhibited the highest activity at 94.64%. Even at the concentration of $40{\mu}g/mL$, evaluation of anti-proliferating activity in YD-10B cells did not reveal any cytotoxic effects. Although MMP-9 expression in YD-10B cells increased after the addition of PMA and thrombin, MMP-2 did not. Additionally, MMP-2/-9 levels in PMA-treated YD-10B cells (i.e., both mRNA expression and protein activation) were highly inhibited in the hexane and chloroform fractions. Compared with MMP-2 levels, MMP-9 mRNA expression and proteolytic activity were inhibited to a greater extent by the hexane and chloroform fractions in thrombin-treated YD-10B cells. Taken together, these results support that thrombin induces tumor invasion through MMP-2/9 and suggest that the L. miniatus may act as an effective functional food, conferring anti-oxidative, anti-thrombotic and anti-cancer activities.

• Asian Pacific Journal of Cancer Prevention
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• 제15권7호
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• pp.2993-2999
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• 2014

Saccharina japonica is a family member of Phaeophyceae (brown macro-alga) and extensively cultivated in China, Japan and Korea. Here, the potential anti-cancer effect of n-hexane fraction of S. japonica was evaluated in SK-Hep1 human hepatocellular carcinoma cells. The N-hexane fraction reduced cell viability and increased the numbers of apoptotic cells in a both dose- and time-dependent manner. Apoptosis was activated by both caspase-dependent and independent pathways. The caspase-dependent cell death pathway is mediated by cell surface death receptors and activated caspase-8 amplified the apoptotic signal either through direct activation of downstream caspase-3 or pro-apoptotic proteins (Bad, Bax and Bak) subsequently leading to the release of cytochrome c. On the other hand, caspase-independent apoptosis appeared mediated by disruption of mitochondrial membrane potential and translocation of AIF to the nucleus where they induced chromatin condensation and/or large-scale DNA fragmentation. In addition, the n-hexane fraction induced endoplasmic reticulum (ER)-stress and cell cycle arrest. The results suggested that potential anti-cancer effects of n-hexane extract from S. japonica on SK-Hep1 cells.

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 1999년도 Pre-symposium of the 10th ISWPC Recent Advances in Paper Science and Technology
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• pp.254-258
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• 1999

The distribution of anionic groups in the fibers, the fines, the colloidal fraction and the dissolved fraction, respectively, of thermomechanical pulp (TMP) suspensions was determined and peroxide bleaching of spruce TMP were also studied. Spruce TMP was extracted with hexane, treated with alkali, or bleached with peroxide. Suspensions made at pH 5.5 were fractionated into long fibres, large fines, small fines, a colloidal fraction and a dissolved fraction. The charge of the fractions was determined using polyelectrolyte titration. To determined the origin of the charges, the contents of fatty acids, resin acids and acidic units in hemicelluloses in the different fractions were determined by has chromatography. Extraction of TMP with hexane prior to fractionation increased the measured charge of the fibres. The removal of the wood resin probably uncovered some carboxyl groups on the fibre surfaces, or improved th e penetration of polybrene into the pores of the fibres. The charge of the fines and the colloidal fraction was lower when the wood resin had been removed. Alkaline treatment of the TMP increased the charge of the fibres and fines, mainly because of demethylation of pectins. Alkaline treatment increased the charge also of the dissolved fraction, because of the release the charge also of the dissolved fraction, because of the release of pectic acids into the water phase. Alkaline peroxide bleaching further increased the charge of fibres and the dissolved fraction, most likely because of lignin oxidation. The charge of the colloidal fraction, consisting mainly of wood resin, was only slightly affected by alkaline treatment and peroxide bleaching. The anionic groups in TMP suspensions were mainly free uronic acids in the hemicelluloses. The contribution from the fatty and resin acids was substantial only for the colloidal fraction.

• 한국산림과학회지
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• 제87권2호
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• pp.260-269
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• 1998

본 연구는 전통적인 민간요법으로 많이 이용되고 있는 옻나무의 옻칠액의 주성분인 urushiol의 암세포증식억제 효과를 검정하기 위하여 L1210(mouse 혈액암세포), PC-9(인체폐암세포), A427(인체폐암세포), KATO III(인체위암세포)에 대한 urushiol의 세포독성을 검정하였다. Urushiol은 column chromatography에서 hexane과 acetone을 혼합하여 step-wise법으로 용출한 분획을 암세포주 L1210, PC-9, A427, KATO III에 투여했다. 그 결과 암세포 증식억제효과는 hexane : acetone(9 : 1) 분획이 가장 높았고, 그외 50%억제농도($IC_{50}$)는 A427 에서 $0.018{\mu}g/m{\ell}$로 가장 우수하였다. 이들 암세포에 대한 urushiol의 hexane: acetone(9 : 1)분획의 50%억제농도($IC_{50}$)를 시판용 tetraplatin 비교한 결과 urushiol이 tetraplatin에 대하여 L1210에서 3.4배, PC-9 에서 3.9배, A427에서 105.5배의 낮은 농도에서도 동일한 억제효과를 나타냈고, KATO III에서는 다소 높았다.

• 한국식품과학회지
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• 제34권1호
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• pp.123-127
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• 2002

Echinacea angustifolia의 줄기와 뿌리 methanol 추출물과 용매별 분획물의 인간유래 백혈암세포인 HL60과 폐암세포인 3LL 세포를 대상으로 항암 활성을 검색한 결과는 다음과 같다. HL60 세포의 경우에 뿌리 추출물은 저농도(0.125 mg/mL)에서 ethylacetate, acqueous, buthanol 분획물의 순으로 $77%{\sim}72%$의 세포독성효과를 나타내었고, hexane 분획물이 저농도에서는 독성효과가 낮았으나 점진적으로 독성효과가 커짐(1.0 mg/mL, 82%)을 알 수 있었다. 3LL 세포주에 대해서는 뿌리의 경우 저농도(0.25 mg/mL)에서 buthanol, ethylacetate 분획물이 $43.5%{\sim}26.3%$의 낮은 세포독성효과를 나타내었고, 1.0 mg/mL 농도에서는 chloroform, hexane 분획물이 $68.4%{\sim}58.5%$의 세포독성을 나타내었다. 에키네시아의 줄기보다 뿌리의 세포독성이 컸고, HL60이 3LL보다 세포독성이 크게 나타났다.

• 한국식품영양과학회지
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• 제33권5호
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• pp.785-790
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• 2004

배추김치, 갓김치, 부추김치의 용매획분 추출물을 제조한 뒤 항산화관련 물질들의 함량을 측정한 결과 카로테노이드는 갓김치의 hexane층에서, 클로로필 함량은 부추김치의 hexane층에서 가장 높았고, 비타민 C함량은 갓김치의 EtOAc층과 BuOH층에서 가장 높게 나타났다. 총 폐놀화합물은 부추김 치의 CH$_2$Cl$_2$층과 EtOAc층, 배추김치 CH$_2$Cl$_2$층, 갖김치 EtOAc층과 CH$_2$Cl$_2$층에 많이 함유되어 있었다. DPPH법을 이용한 유리기 소거능을 조사한 결과 부추김치 CH$_2$Cl$_2$층과 EtOAc층, 배추김치 CH$_2$Cl$_2$층, 갓김치 EtOAc층에서 효과가 큰 것으로 나타났다. 김치종류별 용매획분을 hairless mouse의 피부균질액에 첨가하여 자외선 B를 조사한 후 지질과산 화물 함량을 측정한 결과 부추김치의 CH$_2$Cl$_2$층과 EtOAc층, 배추김치, 갓김치의 EtOAc층에서 낮게 나타나 항산화성이 높은 획분에서 피부의 광산화 억제효과가 큰 것을 알 수 있었고 김치 용매획분을 크림에 첨가하여 hairless mouse의 등에 직접 도포한 후 자외선 B를 조사하였을 때 대조군에 비해 갓김치의 hexane층, 부추김치의 hexane층과 BuOH층을 제외한 모든 용매획분이 피부홍반의 생성을 억제하였고 특히 갖김치 EtOAc층, 배추김치 CH$_2$Cl$_2$층, 부추김치 물층에서 피부보호효과가 큰 것으로 나타났다.

• 한국수산과학회지
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• 제49권5호
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• pp.678-682
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• 2016

Antibacterial activity of 80% methanol extract from 13 commercial herb medicines was measured against fish pathogens Streptococcus iniae, causing several diseases in various kind of fish. Siegesbeckia pubescens showed the strongest antibacterial activity against S. iniae. Methanol extract of S. pubescens was further extracted using several organic solvents having different polarity. Extract from n-hexane and ethyl acetate fraction showed strong activity. Minimal inhibitory concentration, MIC of S. pubescens extract was measured and resulted showing 8 μg/mL with n-hexane fraction and 32 μg/mL with ethyl acetate fraction against S. iniae. The growth of S. iniae was fully inhibited by adding 50 μg/mL (final concentration) of n-hexane or ethyl acetate fraction in the liquid media. It is needed that, from these results, purification and isolation of responsible active compound(s) of these activities and further study on the synergy effect using combination with commercial antibiotics against fish pathogenic bacteria.

• Biomolecules & Therapeutics
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• 제4권4호
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• pp.437-442
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• 1996

Hypoglycemic effect of Tabebuia avellandae was investigated in the streptozotocin(STZ)-induced diabetic rats. Diabetes was induced in male Sprague-Dawley rats by injections of STZ (45 mg/kg, i.v.). Rats weighing 200-250 g were divided into 6 groups: normal, STZ-control, hexane fr., CHCl$_3$fr., BuOH fr. and $H_2O$ fr. group. Normal and STZ-control rats received 3% Tween 80 only. Four groups of diabetic rats were administered orally at doses of 100, 400, 300 and 400 mg/kg/day of hexane, CHC1$_3$, BuOH and $H_2O$ fr. respectively. Fractions were administered orally to the rats for 7 days after STZ injection. All rats were anesthetized with ether, blood samples were taken by cardiac puncture for clinical chemistry and the rats were killed by exsanguination. Liver, kidney, heart and spleen were removed, weighed and analyzed. We measured glucose, protein, cholesterol and triglyceride levels in the plasma and glycogen, cholesterol and triglyceride levels in liver. The extent of blood glucose decrement in rats administered $H_2O$ fraction was greater than that in the STZ-control rats. The serum cholesterol and triglyceride levels were significantly lowered by administration of $H_2O$ fraction compared with those of STZ-control group. Treatment of rats with Tabebuia avellandae fractions caused decreases in STZ-induced elevation of cholesterol and triglyceride. Liver triglyceride level was significantly lowered hexane and BuOH fraction group compared with STZ-control group. These results suggest that $H_2O$ fraction of Tabebuia avellandae has the hypoglycemic action against STZ-induced diabetic rats.