• 대한한방내과학회지
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• 제34권4호
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• pp.375-383
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• 2013

Objectives : This study was performed to investigate the metabolic site of some medicinal herbs in the liver associated with CYP (Cytochrome P450). Methods : Cytochrome P450 is the major enzymes involved in drug metabolism and bioactivation. CYP3A4 and CYP2D6, the major CYP isoforms in humans, catalyse the major proportion of drugs available on the market. Scintillation proximity assay (SPA) is often used in studies to identify compounds that inhibit CYP3A4 and CYP2D6. 28 herbal extracts and radioisotopes were attached competitively to SPA beads, and followed by measuring the remaining radioisotopes in the medium. Erythromycin and dexamethasone, inhibitors of CYP3A4 and CYP2D6, were used as controls respectively. Results : Most of the 28 herbal extracts showed dose-dependent affinity to the CYP3A4 while some of the herbs showed affinity to the CYP2D6. Conclusions : These results suggest that most of the 28 herbal extracts are metabolized safely in the liver, combined with CYP3A4 and CYP2D6.

• 대한한의학방제학회지
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• 제16권1호
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• pp.95-107
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• 2008

Objectives : Hwangnyeonhaedok-tang(HNHDT) is the one of well-known traditional herbal formula and has been widely used for the treatment of various diseases in Korea. For the quality improvement of commercial traditional herbal formula, we investigated the quality of prepared HNHDT. Methods: We measured the contents of baicalin, berberine and geniposide in the HNHDT Ex preparations on the market by HPLC and compared with the official notice of ingredients. Results: In 3 preparations of HNHDT covered with the national health insurance(NHI), the content of baicalin was various from 32.83mg to 185.05mg, the total berberine was $12.0{\sim}50.75mg$ and geniposide was $12.57{\sim}44.84mg$. Contents of marker ingredients in NHI Preparations were below the standard and showed a large variation. In 7 non NHI Preparations of HNHDT and the standard(STD) HNHDT manufactured in laboratory, the content of baicalin was $37.16{\sim}115.69mg$, the total berberine was $5.62{\sim}28.43mg$ and geniposide was $10.98{\sim}113.47mg$. But, because composition of non-NHI preparations is different from NHI preparations, every maker's contents were up to the notice for baicalin and berberine. There was newly enforced standard regulations of geniposide in non-NHI preparations, so contents of geniposide was up to that standard only in JI and STD. Conclusions: There was a large variation of contents in the HNHDT Ex preparations on the market and the quality differences were existed between NHI and non-NHI preparations of HNHDT. Further studies for evaluation of efficacy differences and standardization of manufacturing processes are necessary.

• Natural Product Sciences
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• 제6권3호
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• pp.151-153
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• 2000

Kampilla is an important herbal drug of indigenous system of medicine. Fruit dust of Mallotus philippensis Muell-Arg constitutes the genuine drug Kampilla. However, due to limited distribution of this plant and its high medicinal value, the drug is heavily adulterated with the cheaper substances. Hence the present study was undertaken to evaluate both the authentic and market samples. The drug consists of resin glands and trichomes. Resin glands are lined by a delicate yellowish thin membrane and bear a pore at the tip. Quantitative standards of the drug powder showed 82.50% yield in total ash while alcohol soluble extract of market and authentic sample exhibit 0.97% and 3.458% respectively. The main adulterant noticed in the market sample is brick powder. Simple methods are suggested to identify the genuine drug.

• 한방재활의학과학회지
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• 제28권1호
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• pp.133-144
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• 2018

Objectives Since the health insurance coverage for traditional Korean medicine is very low, some physicians and researcher have suggested that government's institutional support is needed for korean medicine field. Therefore We examine the use of traditional medicine and western medicine in Taiwan, which operates a similar health insurance system to Korea. Methods We selected several studies from Pubmed and NHIRD, that could be used to numerically evaluate the use of traditional medicine. We reviewed the current status of medical use in Taiwan and compare it with that of Korea. Results Through a total of 87 studies, We found that 26.59%~31% of Taiwanese use Traditional medical institutions more than once a year, and the use of traditional medicine has been increasing since 2000. In adults than children, in women than men, the use rate of traditional medicine was high. Especially, herbal medicine was the most common intervention, accounting for 70% of traditional medical care. Conclusions The core of low insurance coverage for traditional Korean medicine is in lack of coverage for herbal medicine. Taiwan's case shows that the unmet demand for traditional Korean medicine of the Korean population can exist widely.

• Advances in Traditional Medicine
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• 제7권3호
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• pp.211-216
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• 2007

Swollen and painful extremities have been treated with herbal baths in attempts to alleviate the symptoms. A popular herbal bath used in China, contains a component called 'bone-penetrating herb' which is believed to facilitate the penetration of herbal substances across the skin to the swollen site, so swollen site, so that pain and swelling will be improved. A search from the Chinese pharmacopedia revealed that 22 different herbs have been traditionally used as 'bone-penetrating herb'. Five of these herbs were available in market and were chosen for experimental studies. Standard diffusion experiments were done to identify the most effective herb among the five, in the penetration facilitation. Glechoma longituba at a concentration of 20% was found to give the best results in the facilitation of Bromophenol blue diffusion across artificial and biological membranes. When compared with one commonly used diffusion facilitator, viz. azone, azone was found to be more effective than glechoma longituba. The encouraging observations support future studies on the basic science behind the use of herbal components as topical agents to treat pain and swelling.

• 식물병연구
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• 제14권3호
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• pp.205-209
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• 2008

배 만수 품종에서 발생되는 수확 후 과피 갈변 발생원인을 구명하기 위해 2년간 시험한 결과를 요약하면 다음과 같다. 배 만수 품종의 과피 갈변은 수확 후 선과시 발생되는 미세한 물리적 상처 부위에서 갈변이 발생되었다. 따라서 과피에 인위적 상처를 낸 후 품종간 과피 갈변 정도를 비교한 결과 신고 품종에 비해 만수 품종이 과피 갈변 발생정도가 심하여 품종간 차이를 나타내었다. 또한 만수 품종은 신고 품종에 비해 과피 조직이 얇았으며, 과피내황산화능이 높고 함량이 많을수록 과피 갈변 발생이 적었다. 결과적으로 만수 품종의 과피 갈변은 수확후 선과주에 발생하는 물리적인 미세상처 발생을 억제하기 위해 수확 후 봉지를 씌운채 선과하면 과피의 상처 발생감소로 갈변 발생이 현저히 억제되었다.

• 한국환경과학회지
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• 제13권12호
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• pp.1109-1115
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• 2004

This study was investigated the residual contents of sulfur dioxide on the 373 kinds of herbal medicine distributed from Korea, China, and Japan. A modified Monier-Williams method was described for the determination of $SO_{2}$ contents in herbal medicines. The residual contents of $SO_{2}$ were not detected at 221 products$(59.8{\%})$ in total 373 products. Regardless of region, $SO_{2}$ contents were not found at Farfarae Flos, Zizyphi Spinosi Semen, Castanea crenata, and Strychni Ignatii Semen. But it's found at Asparagi Radix, Codonopsitis Radix, Lilii Bulbus, and Kaempferiae Rhizoma of every region collected the samples. Also, $SO_{2}$ contents were not detected at the herbal medicines which collected cultural fields of dometic. $SO_{2}$ contents ranged 11$\~$3990 mg/kg(mean 152 mg/kg) at domestic samples, 11$\~$3440 mg/kg(mean 603 mg/kg) at imported samples. After treated with water wash and hot water extraction, the reduction rates of sulfur dioxide were appeared with $24.4{\%}\~68.7{\%}\;and\;83.8{\%}\~100.0{\%},$ respectively. These data will be used to establish a criteron of residual sulfur dioxide in herbal medicines.

• 한국버섯학회지
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• 제16권4호
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• pp.239-249
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• 2018

The global mushroom industry has grown rapidly in recent years in terms of beneficial effects, market value, and demand. India has a wide range of agro-climatic conditions and is largely an agricultural country with a cultivated area of about 4.37 %, generating about 620 million tons of agro waste annually. Mushroom cultivation not only helps recycle agro wastes, but also fills the nutritional gap prevalent among a large population of India. Recently, government industrial policy and creative innovation has promoted research and other endeavors aiming towards the cultivation of mushrooms. Mushroom cultivation in India was initiated in Solan, in the mid-sixties. Mushroom cultivation has been successful in temperate regions of the Himalayas, the Western Ghats, and the hills of northeast India. Recently, many unemployed people have begun to adopt mushroom cultivation as a means of self-employment. It is high time that Indian mushroom cultivators and consumers became aware of the nutritional and medicinal values of cultivated and wild species of mushrooms. The total mushroom production in India between 2010 and 2017 was approximately 0.13 million tons, accounting for a 4.3% increase in the average growth rate of mushrooms per annum. In particular, the total production of white button mushrooms is the highest, with a share of about 73% of total mushroom production. In this review article, we have analyzed the current scenario of the Indian mushroom industry and its contribution to the economic growth of the country.

• 한국버섯학회지
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• 제19권3호
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• pp.256-259
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• 2021

현재 양송이 품종의 개발은 1980년대에 개발된 방법에 의존하여 진행되고 있다. 유전자가위를 이용한 유전자교정 기술이 다양한 분야에서 각광받고 있고, 이 기술을 버섯 육종에도 적용하기 위하여 진행된 이 연구에서는, CRISPR/Cas9 활용에 필수적인 원형질체 분리 효율을 1.0 × 10⁸/mL까지 안정적으로 끌어올렸고, spermidine을 이용하여 PEG 형질전환의 효율 또한 기존 방법에 비해 100배가량 끌어올렸음을 보고한다.

• 대한본초학회지
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• 제35권1호
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• pp.9-18
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• 2020

Objectives : We tried to establish standards for genuine by discriminating 3 kinds of pollen and spore herbs that are highly to be mixed with each other. Using an scanning electron microscope, we prepare a standard for discriminating external morphological characters of minute herbs, thereby contributing to the stable supply of genuine herbal medicines. Methods : Standard samples were confirmed by literature review on external morphological characteristics of original plants and herbal medicines, and collection and identification of original plants. The herbal medicines on the market were purchased and classified with using naked eye observation and magnifier. Finally, micromorphological identifications were conducted using an scanning electron microscope. Results : 1. Pini Pollen was clearly distinguished by its relatively medium size and a pollen grain with two swollen reticulate sacci at both ends. The verrucate ornamentation on the exine surface of the corpus and a sunken leptoma germ pore may be used as a discrimination criteria. 2. Typhae Pollen was distinguished by its relatively small size and a saccus on the end of a pollen grain. Reticulate ornamentation of exine surface of the corpus, and a slightly clear ulcerate germ pore can be used as a discrimination criteria. 3. Lygodii Spora was distinguished by its relatively large triangular-ovate shape and trichotomous fissure. Verrucate-tuberculate ornamentation of exine surface and trilete aperture could be used as a discrimination criteria. Conclusion : These results indicate that the use of electron microscopy is very effective for discriminating the external morphology of minute herbal medicines.