• The Plant Pathology Journal
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• v.36 no.4
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• pp.323-334
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• 2020

Lysin motif (LysM) proteins are reported to be necessary for the virulence and immune response suppression in many herbaceous plant pathogens, while far less is documented in woody plant pathogens. In this study, we preliminarily characterized the molecular function of a LysM protein LtLysM1 in woody plant pathogen Lasiodiplodia theobromae. Transcriptional profiles revealed that LtLysM1 is highly expressed at infectious stages, especially at 36 and 48 hours post inoculation. Amino acid sequence analyses revealed that LtLysM1 was a putative glycoprotein with 10 predicted N-glycosylation sites and one LysM domain. Pathogenicity tests showed that overexpressed transformants of LtLysM1 displayed increased virulence on grapevine shoots in comparison with that of wild type CSS-01s, and RNAi transformants of LtLysM1 exhibited significantly decreased lesion length when compared with that of wild type CSS-01s. Moreover, LtLysM1 was confirmed to be a secreted protein by a yeast signal peptide trap assay. Transient expression in Nicotiana benthamiana together with protein immunoblotting confirmed that LtLysM1 was an N-glycosylated protein. In contrast to previously reported LysM protein Slp1 and OsCEBiP, LtLysM1 molecule did not interact with itself based on yeast two hybrid and co-immunoprecipitation assays. These results indicate that LtLysM1 is a secreted protein and functions as a critical virulence factor during the disease symptom development in woody plants.

• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.281-287
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• 1999

Codonopsis lanceolata ("Deoduck" in Korea) is a perennial herb, and belongs to family, Campanulaceae. Its taproot is used a good source of a wild vegetable as well as an herbaceous medicine. In this study, to develop a bialaphos-resistant transgenic Codonopsis, seed germination mechanism and somatic embryogenesis of the plant were investigated, and Agrobacterium-mediated transformation with bar gene encoding phosphinothricin acetyltransferase (PAT) was performed. Attempt were made to regenerate plant from cells via somatic embryogenesis. When the cotyledons, nodes and leaf disks were cultured on MS medium containing 2,4-D and zeatin, embryogenic calli were induced. Upon transferring the somatic embryos to N6 solid medium without plant growth regulators, they developed into plantlets under continuous illumination. All plants were dead on MS basal medium containing 10 mg/L phosphinothricin (PPT) and Basta, respectively. The explants did not produce calli in the medium containing 200 mg/L kanamycin. The explants were cocultured with Agrobacterium tumefaciens for 2 days, and transformants were selected in MS basal medium containing 1.0 mg/L 2,4-D, 100 mg/L kanamycin and 500 mg/L carbenicillin. After the selection, embryogenic calli were induced and then somatic embryos were produced by subsequent subculturing. The somatic embryos were germiated on N6 basal medium containing 200 mg/L kanamycin and 500 mg/L carbenicillin. PCR analysis showed that nptII and bar genes were introduced in the Deoduck transformants. After the confirmation of bar gene expression in RNA and protein level, the transgenic Deoduck will be used to study the genetics of filial generation with the herbicide control gene, bar.gene, bar.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.4
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• pp.11-23
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• 2018

The purpose of this study is to identify the ecological characteristics and biological interactions between species of the abandoned rice paddy field in mountainous areas and to suggest a management strategy for stable food chain formation and biodiversity enhancement. The study site is located in Uldae wetland of Songchu district Bukhansan National Park, site characteristics and biological habitat characteristics were identified through site survey and literature survey. With regard to physical environment, among geographical features, the Uldae Wetland and the neighborhood inside the basin was a gently sloping area($5{\sim}15^{\circ}$). And 64.0% of basin faced the north. With regard to water environment, the Uldae Wetland was wetland of rainfed paddy field depending on precipitation and the system of stream flowing into the wetland from valley. According to the results of examining flora in plant ecology, in general, they were herbaceous wetland species. 88.6% of existing plants inside the Uldae Wetland basin was a forest in the mountain. And Quercus spp. community and Pinus densiflora community accounted for 64.6% of that, and was dominant. Except for that, Salix koreensis community was distributed. The existing vegetation of Uldae Wetland inhabited wetland species and terrestrialization indicator species, and it was thought that partial terrestrialization inside the Uldae Wetland was in progress after the discontinuation of paddy cultivation, such as the expansion of Salix koreensis distribution area. In the status of appearing faunae in the Uldae Wetland with regard to wildbirds of appearing principal species, The Uldae wetland was based on a abandoned rice paddy field various wildlife, and was a wildlife feeding, spawning, and resting place. The water environment was an important factor in maintaining the wetland living creatures function, habitat of waterbirds and benthic macroinvertebrates, amphibians and odonate are spawning ground and habitat, it was affecting the vegetation ecosystem based on wetlands. In order to maintain the diversity of wildlife, it was important to maintain smooth water supply and water level. A stable food chain will be formed and the Uldae wetland biodiversity will be abundant by establishing the relationship between the species of Uldae wetland, which is abandoned rice paddy field, and the habitat environment favored by species belonging to the ecosystem stepwise linkage. The ecological characteristics of the Uldae wetlands and the relation between the species were analyzed and the environmental conditions were reflected in the planning and management plan of Uldae wetland ecology.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.10 no.6
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• pp.130-138
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• 2007

To protect the surface of ground-cutting slopes occurring at the national road substitute detour [roundabout road]construction jobsite between Munduk and Wubokgu within the jurisdiction of Pohang-si and environment-friendly road construction, a test execution of re-vegetation measures on the major slopes was done and the results are as follows : As a result of finding out the number of sprouting individuals on the test construction site, the average number of sprouting individuals on the three test spots by the method of revegetation measures D, revegetation measures A, and revegetation measures B was 1,292 number of per square meter, 1,190 number of per square meter, and 1,095 number of per square meter respectively, which statistics were higher than those of test spot by the revegetation measures C. As a result of finding out living plant's breeding index [weight in dried state], the average dried weight of living plant at the test spot by the method of revegetation measures B, in case of foreign herbal species, was the highest marking 8.96 grams per square meter, and the next was 6.86grams per square meter by the method of revegetation measures D, and the next after was 6.80 grams per square meter by the method of revegetation measures A, and the last 5.93 grams per square meter by the method of revegetation measures C. As a result of finding out the covering degree on the slopes, the same average covering degree of 80% and 77.5% in revegetation measures A and revegetation measures D individually, which showed a somewhat higher covering degree than those of revegetation measures B and revegetation measures C. As a result of finding out appearing plants on the test construction site, seeding plant life was found to be sprouting on all test spot while native and foreign herbal species and herbaceous plant as well as shrubs were growing in good harmony with each other. However, in case of revegetation measures B and revegetation measures C method, foreign plant species are dominantly growing. As a result of inspecting rifts on the slopes and the excavated state by water, there existed cracks in some of base materials only in revegetation measures C method applied spot.

• Asian Journal of Turfgrass Science
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• v.18 no.1
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• pp.15-27
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• 2004

For the study of ecophysiological characteristics of ground cover plant with shade tolerance, the material productivity and the growh characteristics were analyzed in Hosta clausa community at experimental farm of Kemyung College from March to October. The installation of experimental plots were split into control plot (full sunlight), 30%, 50%, 70%, shading rate by completely randomized design method. After the transplant, to take into account the edge effect of plant population, the plants were selected from each plot by random sampling every 10days. The following results were obtained; Then number of leaf, length of leaf and width of leaf length of leafstack remarkably showed high tendency under 30%, 50% shading plot as compared with control, 70% shading plot for the exmination as the process of develpment. They showed characteristics as herbaceous ground with shade tolerance under conparatively low the light condition and they ettain a stabilized development.

• Korean Journal of Environment and Ecology
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• v.25 no.3
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• pp.404-411
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• 2011

The purpose of this study was to analyze the characteristics of plant covering in the extensive green roof site. Eighteen herbaceous perennial grass such as Caryopteris incara and sedum species such as Sedum Kamtschaticum were planted on an experimental green roof with 10cm substrate depth in 2007. This study investigated vegetation change over 3 growing seasons 2007-2009. The covering rates of planted species mostly increased in 2008, but declined in 2009 except 6 species such as sedum specis and Thymus quinquecostatus var. japonica etc. There were four categories of covering characteristics generated from the results of this study. These include the type of sustain planting site, the type of encroach adjacent site, the type of creeps and spreads, and the type of scatters and spreads. The covering models of eight planted species were drawn by simple regression analysis. However more monitoring of various plants will be needed to establish the information for sustainable roof planting plan.

• Journal of Korean Society on Water Environment
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• v.33 no.5
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• pp.511-524
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• 2017

This study was carried out for the purpose of creating a restoration plan to improve the ecological quality of the Changwon and Nam streams. Based upon the results of comprehensive diagnostic assessment, restoration priority was given to the upstream reach, where conservation status is relatively superior. Restoration level was usually determined to practice active restoration as conservation, and the states of both Changwon and Nam streams were not so good. Restoration plans, by reach, were classified into "upstream", "midstream", and "downstream" were suggested in both terms of horizontal section frame and vegetation-based on the result of diagnostic assessment and the reference information. "Upstream", "mid-stream" and the "downstream" of Changwon and Nam streams were classified into "small-gravel- mountainous", "small-sand-plain", and "small-clay-plain streams" respectively (based on scale, and substrate and slope of river bed). The spatial arrangement of vegetation was laid out in diagram form by reflecting micro-topography and the water level of the horizontal section of river. Information regarding species composition was recommended as dominant species, which appear frequently in three vegetation zones composed of herbaceous plants, shrubs and trees and sub-tree- dominated zones divided by reflecting disturbance regime, depending on position on the horizontal section of river. Moreover, there have been prepared not only plans to improve the terrestrial ecosystems around the streams but also plans to create ecological networks, which can serve to improve the ecologic quality of the whole regional environment by serving to connect streams and terrestrial ecosystems, a process probably necessary and definitely recommended to realize true (genuine) restoration. Plans for ecological parks and networks were prepared by mimicking the species composition of Alnus japanica community, Zelkova serrata community, Carpinus laxiflora community, Quercus aliena community, and Q. serrata community.

• Journal of the Korean Institute of Landscape Architecture
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• v.39 no.2
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• pp.91-102
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• 2011

This study was carried out to analyze the problems on several steps of the establishment of the Nature Ecological Forest in Yeouido Park, Seoul, and also to suggest improved approaches on each step. For execution drawing, planting models and plans seemed to be uncertain, and the quantity and size of planting trees seemed to be impractical. For construction, the woody plants planted on the site were different in species and size from the planting plan. Ecological planting was somewhat limited because of the inappropriate soil properties. For management, replacement of the dead trees was not executed properly, and no management scheme was prepared after the replacement period. We suggested improved approaches for the establishment of ecological forests in urban areas as follows: for execution drawing, overstory, understory and herbaceous ground cover layers should be composed based on standard plant community structures. Trees that are available from tree markets should be specified in the planting plan. For construction, trees for planting need to be tagged to identify species and size. When tree species and size are changed, they should be checked to ensure that they are proper to the plant community model. Soil information should be collected to check that they fit the target plant community model. For management, the proper amount of trees needs to be specified in the planting plan by applying regular discount rates, especially for trees supplied from the government sector. The replacement period should be extended from two years to five years. The change of plant communities should be monitored during first five years after establishment.

• Korean Journal of Plant Taxonomy
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• v.40 no.3
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• pp.135-144
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• 2010

This study intended to investigate environmental factors including soil and vegetation in order to understand the environmental and ecological characteristics of four different habitats of Abelia tyaihyoni. These habitats, according to investigations, are mostly located at elevations of 203 m to 297 m with angles of inclination ranging from 9 degrees to 17 degrees. The litter depth of habitats is 2 cm to 5 cm. A total of 113 vascular plant taxa are identified in seven quadrates of the four habitats. The life form of 113 species is H-$D_4$-$R_5$-e type. The importance value of Abelia tyaihyoni is 39.61%, and 4 highly ranked species such as Quercus dentata (6.27%), Spiraea blumei, Spiraea prunifolia for. simpliciflora (4.04%) and Ulmus davidiana var. japonica (3.84%) are considered to be an affinity with Abelia tyaihyoni in their habitats. The dominant species of woody plants in the four habitats are represented as Pinus densiflora (21.22%) and Quercus dentata (16.82%) in the subtree layer (T2), and Carex humilis var. nana (18.95%) and Carex lanceolata (17.63%) in the herbaceous layer (H). The degree of their average species diversity is 1.42, and that of dominance and evenness are 0.07 and 0.86, respectively. The type of soil is sandy loam, clay loam and loam, and the average field capacity of soil is 22.49%. Their average organic matter is 9.39%, soil pH 6.75, and available phosphorus is $1.23 {\mu}g/g$.

• Journal of Life Science
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• v.31 no.7
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• pp.677-685
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• 2021

Cannabis sativa L. belongs to the Cannabaceae family and is an annual herbaceous flowing plant. The plants can be classified into narcotic marijuana and nonnarcotic hemp. Different parts of C. sativa L. have been used as food, medicine, cosmetics, fiber and textile. However, the use of leaf, flower, and seed of C. sativa L was forbidden in Korea in January 1977 as a result of the Cannabis Control Act due to the narcotic properties. The plant's mature stems have limited uses for the production of fiber and sheets. Recently, various cannabinoids, terpenes and essential fatty acids were identified from C. sativa L., and their safety and useful bio-activities, such as neuroprotective, anti-inflammation, antithrombosis, antiepileptic, and antimicrobial activities, and the relief of pain, have been highlighted. Furthermore, the process of reduction of tetrahydrocannabinol, a representative narcotic compound, and the isolation of cannabidiol, a nonnarcotic active compound in C. sativa L., have been determined. These findings resulted in the legalization of C. sativa L. in Korea for medical use in December 2018 and the exclusion of C. sativa L. from the narcotic list of the UN Commission on Narcotics Drugs (UNCND) in December 2020. Therefore, developments of various high-value added products have commenced worldwide. Additionally, in 2021, the Korean government deregulated special zones based on hemp. In this study, the current status and the prospect of the hemp industry, as well as essential techniques for developing new hemp products, are provided for the activation of the Korea Green-Rush.