• 한국작물학회지
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• 제49권4호
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• pp.354-357
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• 2004

A simplified technique which cryoprotects zygotic embryos by encapsulation-dehydration was developed for the germplasm conservation of herbaceous peony (Paeonia lactiflora Pall.). The highest survival rate $(85\%)$ was obtained from embryos treated by encapsulation-dehydration. The zygotic embryos were precultured on MS medium containing 0.3mg/L $GA_3$ for 1 day. The precultured embryos were encapsulated in $3\%$ (w/v) alginate beads and immersed for 1 h in MS medium containing 2 M glycerol and 0.5 M sucrose. The encapsulated embryos were dehydrated for 5h by air drying prior to direct immersion in liquid nitrogen. This encapsulation-dehydration method appears to be a promising technique for germplasm cryopreservation of a herbaceous peony.

• 한국약용작물학회지
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• 제4권3호
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• pp.187-192
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• 1996

작약(芍藥)의 개화조절(開花調節)을 위(爲)한 기초자료로 삼고자 꽃눈의 분화과정(分化過程)과 동계(冬季) 가온재배(加溫栽培)시의 적정(適定) 가온시사(加溫時斯) 및 편온처리(偏溫處理) 효과(效果)를 조사한 결과는 다음과 같다. 1. 작약(芍藥)의 꽃눈분화 과정을 보면, 6월(月)17일(日) 조사시(調査時) 눈의 분화는 이미 시작되어 있었고, 화기의 분화는 9월중순(月中旬)에, 꽃잎의 분화는 10월(月) 에 관찰되었으며, 이른 봄눈이 지상부(地上部)로 출현(出現)하면서 암술이 분화된 개체가 관찰되었다. 2. 동계(冬季)에 시기(時期)를 달리하여 가온재배(加溫栽培)한 결과, 밀양지역(密陽地域)에서 작약(芍藥)의 촉성재배(促成栽培)시 12月 상순(上旬)부터 가온재배(加溫栽培)하면 낙화(駱花)가 가능하였으며 12월(月) 하순(下旬) 가온낙시구(加溫駱始區)까지 출현소요(出現所要) 일(日) 수(數)가 현저히 감소(減少)하다가 그 후에는 差(差)가 없었다. 3. $5^{\circ}C$에서 1주간(週間) 저온처리를 한 후 가온재배(加溫栽培)를 하면 잎은 출현(出現)하였으나 개화(開花)하지 않았고 2과(過)이상 처리시 개화(開花)하였다. 2주(週) 이상 저온처리시 처리기간에 따른 폐화소요일수(閉花所要日數)의 차(差)는 미미(微微)하였으나 출현경수(出現莖數)와 폐화률(閉花率)은 처리기간이 증가함에 따라 증가하였다. 4. 저온처리후 가온재배(加溫栽培)하면 동일가온재배(同一加溫栽培)시기의 무처리(無處理)에 비해 출현소요일수(出現所要日數)가 크게 감소하였다. 저온처리에 의한촉성재배(促成栽培)의 경우빨리 개화(開花)한 것은 1월(月) 20일(日)이었고, 저온처리를 하지않은 것을 12월(月)에 촉성재배(促成栽培)할 경우 2월(月) 하旬(下旬)까지 개화(開花)가 가능하였다.

• 원예과학기술지
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• 제31권3호
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• pp.322-327
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• 2013

The genetic diversity and phylogenetic relationships of eleven herbaceous peonies grown in Korea were analyzed by random amplified polymorphic DNA (RAPD). Twenty-four decamer RAPD primers were used in a comparative analysis of these Korean peony species. Of the 142 total RAPD fragments amplified, 124 (87.3%) were found to be polymorphic. The remaining 18 fragments were found to be monomorphic (12.7%) shared by individuals of all 11 peony species. Cluster analysis based on the presence or absence of bands was performed by Jaccard's similarity coefficient, based on Unweighted Pair Group Method with Arithmetic Averages. Genetic similarity range was 0.39 to 0.90 with a mean of 0.64. This study offered a rapid and reliable method for the estimation of variability among different peony species which could be utilized by the breeders for further improvement of the local peony species. Also, the results propose that the RAPD marker technique is a useful tool for evaluation of genetic diversity and relationship amongst different peony species.

• Journal of Plant Biotechnology
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• 제29권3호
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• pp.193-198
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• 2002

작약의 약 및 소포자 배양 효율을 향상시키기 위하여 배지 내에 첨가되는 PAA의 농도별 배형성 정도와 배의 형태적 변이 등에 대한 실험을 수행하여 얻어진 결과를 요약하면 다음 과 같다. 작약의 약배양에서 배지 내에 첨가되는 PAA의 농도에 따라 소포자 유래의 배형성률은 다르게 나타났는데 2 mg/L의 PAA가 첨가된 배지에서 배형성률이 가장 높게 나타났으며, PAA의 농도가 증가됨에 따라 배형성를은 낮아진 반면 캘러스 형성률은 증가하는 경향을 나타내었다. 소포자에서 유래된 배의 형태는 PAA의 농도에 따라 큰 변이를 나타내었는데 2개의 자엽을 가진 정상배의 출현빈도는 2 mg/L의 PAA가 첨가된 배지에서 가장 높게 나타났으며 PAA의 농도가 그 이상으로 증가하면 오히려 비정상배의 출현빈도가 높아지는 경향이었다. 작약의 소포자 배양에서도 2 mg/L의 PAA 첨가 배지에서 10일간 전배양된 약을 PAA (2 mg/L)를 함유한 MS액체 배지에 배양했을 때 누출된 소포자 (shed microspore)의 수도 많았고 분열률도 높았으며, 이들 소포자로부터 형성된 배의 수도 가장 많았다.

• 한국자원식물학회지
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• 제31권6호
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• pp.641-651
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• 2018

Floral scents and metabolites from cut flowers of 14 peony cultivars (Paeonia lactiflora Pall.) were analyzed to discriminate different cultivars and to compare the Korean cultivar with the other cut peonies imported to Korea using electronic nose (E-nose) and Fourier transform infrared (FT-IR) spectroscopy combined with multivariate analysis, respectively. Principal component analysis (PCA) and discriminant function analysis (DFA) dendrogram of peony floral scents were not precisely same but there were 3 groups including same cultivars. PCA and partial least squares-discriminant analysis (PLS-DA) dendrograms of peony metabolites showed that different cut peony cultivars were clustered into two major groups including same cultivars. Fragrance pattern of Korean 'Taebaek' was classified to same group with 'Jubilee' on the PCA and DFA results and its metabolite pattern was clearly discriminated by the PCA and PLS-DA compared to the other cultivars. These results show that the 14 peony cut flowers could be discriminated corresponding to their chemical relationship and the metabolic profile of Korean 'Taebaek' has distinctive characteristics. Furthermore, we suggest that these results could be used as the preliminary data for breeding new cut peony cultivars and for improving the availability of Korean cut peony in cosmetic industry.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2005년도 춘계학술발표회 요지
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• pp.292-293
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• 2005

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2004년도 생명공학 실용화를 위한 비젼
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• pp.160-160
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• 2004

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2004년도 춘계학술대회
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• pp.64-64
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• 2004

• 한국작물학회:학술대회논문집
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• 한국작물학회 2004년도 춘계 학술대회지
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• pp.92-93
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• 2004

• Journal of Life Science
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• 제12권1호
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• pp.19-21
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• 2002

The pathway of embryos formed anther culture in herbaceous peony was influenced by addition of 2,4-D. MS medium with 2,4-Dichlorophenoxy acetic acid (2,4-D) alone did not arise direct embryogenesis, but was proliferate callus. Embryos through calli were produced on medium containing 0.2 mg/1 zeatin or without growth regulators. Direct embryogenesis was obtained from MS basal medium. However, after the anthers were cultured on medium with 0.1 mg/1 2,4-D, 3 g/1 AC, 30 g/1 sucrose, 2 g/1 gelrite for 40 days. Its efficiency (32.3 %) was markedly improved when anthers cultured on medium without 2,4-D. Embryo morphology was also affected by the 2,4-D used in medium. The induction of normal embryos with two cotyledons was higher in the embryos formed through direct embryogenesis than those formed callus. The embryos formed from calli were mainly showed abnormal embryo with one, three, four cotyledons or hors and bowling pin type.