• 한국식품영양과학회지
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• 제24권5호
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• pp.713-719
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• 1995

본 연구는 3주간 $CCl_4$를 투여한 랫트를 이용하여 각 장기 및 혈액 생화학치의 변화를 조사, 과산화지질가를 감도가 좋은 형광발광 액체 크로마토그래피를 이용해 측정, 간세포를 전자현미경적으로 관찰 등의 방밥으로 간기능에 미치는 PRC의 효과를 알아보기 위하여 실시하였다. 그 결과, PRC투여군은 정상대조군에 비하여 체중은 감소하고, 상대적 간장 중량의 감소경향, GOT 및 GPT의 수치가 감소하였으며, 특히 과산화물가가 유의하게(p<0.001) 감소하였다. 이로 인한 결과를 명확히 뒷받침해 주고 있는 간세포에 대한 전자현미경적 관찰의 결과는 RPC투여의 효과를 간세포의 형태학적 관찰에서 간세포에 대한 PRC의 효과를 나타내 주었다. 이 결과는 칡 카테킨이 사염화탄소로 유도된 간장의 과산화지질의 형성을 방지해서 간기능을 회복시키는데 효과적이라는 것을 시사한다 하겠다.

• Korean Journal of Radiology
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• 제21권10호
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• pp.1115-1125
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• 2020

Combined hepatocellular-cholangiocarcinoma (cHCC-CCA) is a primary liver cancer (PLC) with both hepatocytic and cholangiocytic phenotypes. Recently, the World Health Organization (WHO) updated its histological classification system for cHCC-CCA. Compared to the previous WHO histological classification system, the new version no longer recognizes subtypes of cHCC-CCA with stem cell features. Furthermore, some of these cHCC-CCA subtypes with stem cell features have been recategorized as either hepatocellular carcinomas (HCCs) or intrahepatic cholangiocarcinomas (ICCs). Additionally, distinctive diagnostic terms for intermediate cell carcinomas and cholangiolocarcinomas (previous cholangiolocellular carcinoma subtype) are now recommended. It is important for radiologists to understand these changes because of its potential impact on the imaging-based diagnosis of HCC, particularly because cHCC-CCAs frequently manifest as HCC mimickers, ICC mimickers, or as indeterminate on imaging studies. Therefore, in this review, we introduce the 2019 WHO classification system for cHCC-CCA, illustrate important imaging features characteristic of its subtypes, discuss the impact on imaging-based diagnosis of HCC, and address other important considerations.

• 대한수의학회지
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• 제53권1호
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• pp.11-17
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• 2013

In this study, to understand the pathogenesis of new rabbit hemorrhagic disease virus (RHDVa) serotype, we carried out to administrate RHDVa to rabbits, and to examine sequential electron microscopic changes and relationship between pathogenesis and apoptosis. TUNEL-positive cells began to be observed from 24 hours after inoculation (HAI) and the number of positive cells was slightly increased with the course of time. Whereas marked increase of positive cells was seen in the liver from the rabbits died acutely. Typical viral particles with cup-like projections and a diameter of 30~40 nm were detected in homogenized liver samples and tissues at 36 and 48, and 48 HAI, respectively. Ultrastructurally, glycogen deposition was observed from the first stage of hepatocellular degeneration by RHDVa infection and then, swelling and disruption of cristae of mitochondria by viral particles, swelling of smooth endoplasmic reticulum, vacuoles and vesicles were detected. Condensation, margination and fragmentation of chromatin were observed in degenerative hepatocytes at 36 and 48 HAI, indicating apoptotic bodies. These data offer that hepatocytic apoptosis by RHDV infection could be closely related with mitochondrial impairment in the hepatocytes.

• 한국발생생물학회지:발생과생식
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• 제16권1호
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• pp.9-18
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• 2012

The effects of the estrogenic compound 4-nonylphenol (4-NP) on vitellogenesis in juvenile olive flounder ($Paralichthys$ $olivaceus$) and rockfish ($Sebastes$ $schlegeli$) exposed continuously at 10, 50 and $100{\mu}g{\ell}^{-1}$ levels for 7 days were compared. The expression of VTG mRNA level and protein using specific probes were examined. The levels of plasma estradiol-17 ${\beta}(E_2)$ and testosterone (T) were assessed by radioimmunoassay (RIA). Plasma $E_2$ concentrations increased significantly in two female fish species exposed to $100{\mu}g{\ell}^{-1}$ of 4-NP over concentrations in control fish. Plasma T concentrations increased in $P.$ $olivaceus$. Four days after exposure, the level of VTG mRNA expression increased in $P.$ $olivaceus$ and $S.$ $schlegeli$ exposed to $20{\mu}g{\ell}^{-1}$ of 4-NP. In addition, plasma VTG protein expression was seen in $P.$ $olivaceus$ and $S.$ $schlegeli$. In $S.$ $schlegeli$ and $P.$ $olivaceus$ exposed to 4-NP, the changes were noticed mainly in hepatocytic vaculation after 7 days of exposure. Thus, 4-NP may disrupt vitellogenesis in immature fish both directly and indirectly via disrupted steroidogenesis and liver pathology. Immature $S.$ $schlegeli$ were the most sensitive to 4-NP exposure in vitellogenesis.

• Applied Microscopy
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• 제31권1호
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• pp.37-47
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• 2001

화상에 의한 피부 손상이 간에 미치는 영향을 알아보기 위하여 흰쥐를 이용하여 피부 화상을 유도한 다음 각각 5시간, 24시간 후 생화학적 정량법과 형태학적 관찰을 통해 간 조직 손상의 발병기전을 검토해 보고자 하였다. 흰쥐의 화상 유발은 등쪽면의 털을 깎고(total burn surface area $20\sim25%$) $100^{\circ}C$ 물로 10초간 흡입손상 없이 피부 화상만을 가하였다. 생화학적 정량으로는 혈청 내 xanthine oxidase(XO)와 aniline aminotransferase (ALT)의 활성변화, 그리고 혈장 단백질 함량 변화를 측정하였고, 형태학적 관찰은 혈액 중 다형핵 백혈구 수의 산정과 간 세포의 미세구조 변화를 관찰하였다. 실험 결과, 화상 후 혈청 내 XO의 활성 증가(P<0.01)와 함께 체중 당 간 무게(p<0.05)와 혈청 내 ALT의 활성이 증가되었다. 화상 직후 allopurinol의 복강투여로 XO활성, 간 무게, 그리고 ALT의 활성은 모두 감소되었다. 화상 손상에 의한 간 조직의 미세구조적 변화로는 소포체 종창, 리보솜 탈락, 지방소적의 축적, 그리고 담모세관과 세포간질의 확장이 관찰되었다. 뿐만 아니라 염증세포인 호중구의 침윤과 함께 혈관 내피세포의 손상, 쿠퍼세포의 활성화, 그리고 미세융모의 손상들이 관찰되었다. 또한 혈 중 다형핵 백혈구의 수적인 변화에서 화상 유발 5시간 후에 현저히 감소되어 내부 장기에 호중구 침윤의 가능성을 알 수 있었다. 하지만 allopurinol의 투여로 이러한 미세구조의 변화를 예방할 수 있는 것으로 나타났다. 이러한 결과들을 종합해 볼 때, 피부 화상으로부터 간 손상을 유발하는데 있어서 혈 중 XO의 활성증가가 매우 핵심적인 역할을 담당하는 것으로 사료되었다.

• Toxicological Research
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• 제21권2호
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• pp.141-150
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• 2005

1,3-Dichloro-2-propanol (1,3-DCP) is known as chloride chemicals and causes severe hepatotoxic agent. The Ito cells and Kupffer's cells of the liver in the 5 old F344 Rats were exposed to 1,3-DCP gas chamber for 6 hours/ a day, 5 days/ a week, and 13 weeks, in the 0, 5, 20, 80 ppm, respectively. After then the body weights, liver weights, and relative liver weight to body weight were measured, and the hepatic tissues were prepared by the routine and Immunostain method, and observed by the LM, and EM. In the results, there were severe body weight decrease (p<0.05) in the 80 ppm of the male and female rats. The relative liver weights to the body weight were increased relate with exposed 1,3-DCP concentration (P<0.001). Inflammatory cells, infiltration was observed at the perivascular area in the 20 ppm exposed group, and bilirubin pigment infiltration, bile duct hyperplasia, inflammation hepatocytic necrosis, fibrosis were observed in the 80 ppm exposure group. In the 80 ppm exposure group, disarrangement of the endothelial cells, erythrocytes and hepatic cell fragment in the Disse space and numerous migration macrophages were observed in the necrotic area by EM observation. In the immunostained hepatic tissues positive stained ED1 cells were extremely increased (P<0.05) in central vein area, but ED2 was weakly positive immunostained in the 80 ppm exposed group. Immunostained desmin was observed in the Ito cell. It was no difference in the low and medium exposed group but it was typical increase in the necrotic area. In conclusion, These results suggest that NOAEL of 1,3-DCP may be 5 ppm in rats and the Immunostained of desmin, ED1 and ED2 positive cells activated in the inflammatory liver were related to the exposure volume and density. The increase of the Ito cells were related to the severe phagocytosis of the Kupffer's cells.