• 한국산학기술학회논문지
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• 제20권7호
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• pp.145-150
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• 2019

본 연구는 동물복지 인증기준의 개선을 위해 농가 현장조사를 실시하였으며, 더불어 국내 농가환경에 적합한 동물복지 수준평가 방법을 개발하고자 일부 인증 농가를 대상으로 동물복지 수준의 기초자료를 확보하고자 조사를 실시하였다. 연구조사의 대상은 동물복지 산란계 인증농가 10개소를 선정하였으며, 산란계 인증농가 사육시설 조사는 사육형태, 사육두수, 사료와 음수 공간, 사육밀도, 횃대의 길이 및 모양을 수집하였다. 동물복지 수준 평가는 모래목욕의 유무, 산란상 사용, 환경풍부화물 제공, 방사장 제공, 깃털손상, 쪼기에 의한 상처 유무를 조사였다. 연구결과는 동물복지 산란계 인증농가들의 대부분이 인증기준을 준수하고 있었다. 일부 농가에서 과도한 인증기준을 지적하였는데 실제 농가내부에 급이기, 급수기 및 횃대의 시설로 인해 닭들이 움직일 수 있는 공간이 제한적인 것으로 판단되었다. 모든 농가들에서 산란상을 제공하고 있었으나 방란율이 20%이상인 농가도 있어 산란상 활용에 문제가 있음을 확인하였다. 횃대의 경우 길이는 충족하고 있었으나 10개 농가 중 3개 농가에서만 둥근형태의 횃대를 사용하고 있었다. 모든 농가에서 깔짚을 제공하고 있었으나 보충 또는 교체가 이루어지지 않아 바닥이 단단해져 닭들이 모래목욕행동을 보이지 않는 농가도 2개소나 되었다. 환경풍부화물의 경우 사료 외에 풀사료 등을 제공하는 농가는 4개소로 조사되었다. 대부분의 조사농가에서 깃털손상이 확인되었는데 이것은 조사농가 모두 유정란을 생산하고 있어 수탉에 의한 깃털손상일 것으로 판단된다. 본 연구에서 10개소의 농가만 조사하였기 때문에 전체 인증농가를 대표할 수는 없으나 일부항목에서 조사농가들 사이에서도 큰 차이를 보이고 있어 동물복지 수준평가에 면밀한 분석이 이루어져야 할 것으로 판단된다.

• Animal Bioscience
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• 제37권4호
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• pp.689-696
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• 2024

Objective: Our study examined the impact of propriety blends of Bacillus strain probiotics on the performance, egg quality, and faecal microflora of laying hens. Methods: A total of 183 Institut de selection Animale (ISA) brown laying hens aged 23 weeks with an average body weight of 1,894±72 g were randomly allocated into 3 groups as control (corn-soybean meal based diet, CON), 0.5 g/kg Enterosure probiotics (ET1, 3×10⁸ colony-forming unit [CFU]/kg feed), and 5 g/kg Enterosure probiotics (ET2, 3×10⁹ CFU/kg feed) administered in mashed form. At the completion of each phase hen day egg production (HDEP), average egg weight (AEW), feed intake, and faecal microbiota were evaluated. Results: HDEP and AEW were higher (p<0.05) in the ET2-supplemented diet in phase 3 (week 9 to 12) compared with CON. Egg mass (EM) was higher (p<0.05) in phase 2 at ET2, and also higher (p<0.05) in phase 3 at the ET1 and ET2-supplemented diets compared with CON. Feed conversion ratio was lower (p<0.05) in phase 3 at the ET1 and ET2-supplemented diets, with ET2 being the lowest compared with ET1 and CON. Yolk colour was higher (p<0.05) in the ET-supplemented diets at phase 3 compared with CON. Bifidobacterium spp. was higher (p<0.05) in the ET2- supplemented diet compared with CON in phase 2, while in In phase 3, Lactobacillus spp. and Bifidobacterium spp. were higher (p<0.05) in the ET-supplemented diets compared with CON. Coliforms were lower (p<0.05) in the ET-supplemented diets compared with CON in phase 3. Conclusion: The propriety blends of Bacillus strain probiotics supplements at 0.5 g/kg and 5 g/kg could improve the production and quality of eggs with more significance at 5 g/kg for HDEP, AEW and EM, which was achieved via the increase of beneficial microbiomes such as Lactobacillus spp., Bifidobacterium spp., and the decrease of pathogenic microbiomes like Escherichia coli and Coliforms which was speculated to improve gut barrier function and the reproductive hormone.

• Asian-Australasian Journal of Animal Sciences
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• 제31권5호
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• pp.705-711
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• 2018

Objective: This experiment was conducted to evaluate the effects of golden needle mushroom (Flammulina velutipes) stem waste (FVW), on organic eggs production, calcium utilization, antibody response, serum immunoglobulin, and serum cytokine concentration at early phase of production in laying hens. Methods: A total 210, 19 weeks old aged ISA Brown layers were randomly assigned into 5 equal treatment groups, with 7 replications of 6 hens each. Dietary treatment included a standard basal diet as control; antibiotic (0.05% flavomycin); 2% FVW; 4% FVW; and 6% FVW. The experimental duration was 10 weeks. Results: There was no significant differences (p>0.05) on hen day egg production, egg weight, egg mass, feed intake, and feed conversion ratio (FCR) among experimental groups. Unmarketable eggs were significantly lower (p<0.05) both in 4% FVW and 6% FVW fed groups than control group. The calcium retention and calcium in egg shell deposition were significantly higher (p<0.05) in FVW inclusion groups than control and antibiotic groups. Antibody titers against Newcastle diseases were significantly higher (p<0.05) in 6% FVW fed group (except combined with 4% FVW at day 147) and infectious bronchitis were significantly higher (p<0.05) in FVW fed groups (except 2% FVW and 4% FVW at day 161) than control and antibiotic groups. Serum immunoglobulin sIgA was significantly higher (p<0.05) in all levels of FVW and IgG was significantly higher (p<0.05) in 4% FVW than control and antibiotic groups. Serum cytokine concentration interleukin-2 (IL-2) was significantly higher (p<0.05) in 6% FVW; IL-6 and tumor necrotic $factor-{\alpha}$ were significantly higher (p<0.05) both in 4% FVW and 6% FVW than control and antibiotic groups; IL-4 was significantly higher (p<0.05) in antibiotic, 2% FVW and 4% FVW fed groups than control. Conclusion: F. velutipes mushroom waste can be used as a novel substitute for antibiotic for organic egg production and sound health status in laying hens.

• 한국가축번식학회지
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• 제5권1호
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• pp.36-42
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• 1981

This experiment was carried out to determine the optimum interval between inseminations in artifical insemination of hens. Two hundred and forty hens of Hisex commercial stock at 25 weeks of age and 20 cocks of the Rhode Island Red at 40 weeks of age were used for the experiment, and a total of 6,784 eggs were obtained. The intervals between inseminations compared in this study were: 3 days (T1), 5 days (T2), and 7 days(T3). Mixed raw semen was inseminated and the semen does was 0.03ml per insemination per hen. The inseminations were conducted at 15:00 at each time. The total number of insemination performed was 9 for the T1, 6 for the T2 and 5 for the T3, and eggs were collected over a period of 31 days, 32 days and 35 dyas, respectively. The average egg production of the hens during the experiment was 85.9% and the average temperature during the experiment was around 30$^{\circ}C$. The average sperm count was 3.69 billion per ml. The results obtained in this experiment can be summarized as follows: The fertility over the entire experimental period bythe treatment was 91.7% for the T1, 84.4% for the T2, and 75.2% for the T3. The difference between T1 and T3 in fertility was significant at 5% level. The average fertility on the second, third and fourth day after the insemination in the T2 and T3 was maintained at a relatively high level, but it tended to decline rapidly from the fifth day after the insemination. The average fertility for one week after the last insemination was 88.8% for the T1, 88.8% for the T2 and 78.6% for the T3, and none of the differences among the treatments were statistically significant. On the basis of the results from this study, it is recommended to adjust the insemination intervals within the range from the 3 to 5 days in order to maintain a highest level of fertility in the hens at an early stage of egg production as in the case of the hens used in this experiment. An insemination interval of 3 days is recommended, especially at an initial stage of insemination. For the hens with a low fertility, shortening, of the insemination interval to 3 or 2 days is desirable.

• 한국가금학회지
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• 제29권3호
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• pp.151-156
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• 2002

백색란 계통인 Hy-line W-98과 갈색란 계통인 Hy-line Brown을 이용하여 주요 계란형질들과 산란율에 대한 닭의 계통간의 차이를 구명하고자 시험한 결과, 각 시험주령별 평균 난중에 있어서 백색란 계통과 갈색란 계통간에 유의한 차이는 나타나지 않았으나, 전체 평균 난중은 백색란 계통이 63.57 g 이었고, 갈색란 계통이 64.43 g으로서 갈색란 계통의 계란이 0.86 g이 더 무거운(P<0.05) 것으로 나타났다. 전체 난중에 대한 난백의 비율은 54주령시를 제외한 나머지 모든 주령에서, 그리고 전체 평균 난백의 비율에서도 갈색란 계통은 백색란 계통보다 유의하게 (P<0.05) 더 높은 것으로 나타난 반면에, 난중에 대한 난황의 비율은 모든 시험주령 및 전체 평균치에 있어서 갈색란 계통이 백색란 계통보다 유의하게(P<0.05) 더 낮았다. 한편 난중에 대한 난각의 비율 및 난각의 두께는 갈색란 계통이 백색란 계통보다 유의하게(P<0.05) 더 높거나 더 두터운 것으로 나타났다. 전체 난중에 대한 난황 : 난백의 비율은4개 시험주령중 3개 시험주령에서 갈색란 계통이 백색란 계통보다 유의하게(P<0.05) 더 낮은 것으로 나타났다. 초산시(20주령)부터 56주령시까지의 전체 평균 Hen-day 산란율은 백색란 계통이 63.7%이었던 반면에, 갈색란 계통은 70.3%로서 갈색란 계통이 백색란 계통보다 6.6% 정도 산란율이 유의하게(P<0.05) 더 높았다. 결론적으로 계란소비자가 상대적으로 더 선호하는 갈색란 생산 계통을 사육하는 것이 성계의 체중과 가금티푸스와 같은 질병문제를 개량한다면 난중, 난백의 비율, 난각의 두께 및 산란율에 있어서 백색란 생산계통의 사육보다 더 바람직함을 알 수 있었다.

• 한국응용과학기술학회지
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• 제34권3호
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• pp.577-586
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• 2017

본 연구의 목표는 분변토로부터 분리한 Bacillus subtilis외 2종의 복합 생균제를 이용하여 제조한 발효사료가 산란계 생산성, 계란품질 및 양계장 악취저감에 미치는 효과를 조사하는 것이었다. 산란계 240마리를 4개의 처리구로 구분하여 완전임의 배치하였다. 실험 처리구는 대조구, 분변토발효사료 0.3, 0.5% (FECF3, FECF5), 일반생균제 0.2% (CP)로 구분하였다. 산란율은 일반사료를 섭취한 대조구와 비교할 때 FECF 처리구는 유의하게 높았으나 CP 처리구는 차이가 없었다. 호우유닛, 난각두께, 파란강도, 난황색 지수 및 농후난백 높이는 대조구와 비교할 때 FECF 처리구가 증가하였으나 CP 처리구와 차이는 없었다. 계란의 n-3 함량은 대조구와 비교할 때 FECF 처리구가 높았으나 n-6/n-3 비율은 낮았으며 CP 처리구와의 차이는 없었다. 계분을 실온에서 5일간 방치 후 암모니아 발생량은 대조구와 비교할 때 CP와 FECF 처리구가 낮았다.

• 한국가금학회지
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• 제15권2호
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• pp.73-79
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• 1988

본 시험은 육용종계의 사료급여시간 및 급여누수가 산란율. 난중 사료요구율, 1일 중 산란분포와 종란의 수정율 및 부화율과 난각질 등에 미치는 영향을 조사하기 위하여, 마니커 육용종계 1,248수를 공시하여, 사료의 급여시간 (08：00시와 14：00시)과 급여구수(1일 1회, 1일 2회, 2일 1회)의 효과를 36주간 비교시험 하였는바 얻어진 결과는 다음과 같이 요약된다. 1 육용종계의 산란율과 사료요구율은 사료의 급여시간이나 급여회수에 영향을 받지 않았고, 난중은 42주령이전에는 사료를 오후에 급여함으로서 증가하였으나 (p＜0.01) 42주령 이후에는 사료급여시간이나 급여회수에 따라 차이가 없었다. 2. 1일 중 산란분포는 격일급여구 (시험2)를 제외하고 오후사료급여구는 오전사료급여구에 비해 오전 산란수는 적고 오후산난수는 많은 경향이었다 (p＜0.01). 3 36주령과 60주령에 각각 조사한 종란의 수정률과 부화율은 처리간에 차이가 없었고, 종란의 난각질은 36주령시에는 차이가 없었으나 60주령시에는 사료를 오후에 급여함으로서 난각강도가 증가하였다. (p＜0.05).

• 한국축산식품학회지
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• 제30권4호
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• pp.609-616
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• 2010

This study was conducted to determine the effects of dietary low grade soybean, fermented with Aspergillus oryzae (FSB 1) or Bacillus subtilis var. natto (FSB 2), on egg production and quality, fat and cholesterol content, and the fatty acid (FA) profile of eggs by lipid layer. A total of 18 Hi-Line strain layers, 22 wk of age, were randomly assigned to three dietary treatments: no fermented soybean (control), control with 15% FSB 1 (C + FSB 1), and control with 15% FSB 2 (C + FSB 2). The rate of egg production and egg weight were evaluated between two periods: one was from the 1st to 4th wk and the other was from the 5th to 8th wk. At the 8th wk, a total of 30 eggs were randomly selected from each treatment group and analyzed for physical quality, fat content, fatty acid composition and cholesterol content. The results showed that egg production was increased in hens fed with diets containing fermented soybeans from the 5th to 8th wk period (p<0.01). A similar tendency was observed through eight weeks' cumulative egg production (p<0.05). There were no significant differences in egg production between the C + FSB 1 and C + FSB 2 treatment groups (p>0.05). Egg weight and other physical properties did not vary between treatment groups (p>0.05). Egg yolks among different treatment groups were similar in fat content, but egg yolks in the C + FSB 1 and C + FSB 2 groups had lower oleic acid (p<0.05), higher linoleic, ${\alpha}$-linolenic, and arachidonic acids (p<0.01), and lower cholesterol content (p<0.05) than those in the control group. In conclusion, supplementation of fermented low grade soybeans might be useful as a functional feedstuff to improve egg production and quality for a healthy human diet.

• Asian-Australasian Journal of Animal Sciences
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• 제26권6호
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• pp.831-837
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• 2013

An experiment was conducted to investigate the effects of dietary supplementation of Rhodobacter capsulatus KCTC-2583 on egg-yolk and serum cholesterol, egg production and quality parameters during the late laying periods in hens. A total of 160 Hy-Line Brown layers (54 wk-old) were randomly allotted to 4 treatment groups on the basis of laying performance. Each treatment had 4 replicates with 10 birds each (40 birds per treatment). Two hens were confined individually with cage size $35{\times}35{\times}40$ cm and each 10 birds (5 cages) shared a common feed trough between them forming one experimental unit. Dietary treatments were; basal diet supplemented with 0 (control), 0.05, 0.10 and 0.15% R. capsulatus KCTC-2583. Experimental diets were fed in meal form for 56 d. Dietary supplementation of increasing levels of R. capsulatus KCTC-2583 reduced (linear, p<0.05) egg-yolk cholesterol and triglycerides (d 28, 42 and 56) concentrations. Also, serum cholesterol and triglycerides (d 21, 42 and 56) concentrations were linearly reduced (p<0.05) with increasing dietary R. capsulatus KCTC-2583. Laying hens fed a diet supplemented with increasing levels of R. capsulatus KCTC-2583 had increased (linear; p<0.05) overall egg production, egg weight, egg mass and feed efficiency. However, dietary treatments had no effect (linear or quadratic; p>0.05) on feed intake of laying hens. At d 28 and 56, breaking strength and yolk colour of eggs were linearly improved (p<0.05) in laying hens fed dietary increasing levels of R. capsulatus KCTC-2583. Dietary treatment had no effects (linear or quadratic; p>0.05) on albumin height, shell thickness and shell weight at any period of experiment. These results indicate that dietary supplementation of R. capsulatus KCTC-2583 has the potential to improve the laying hen performance and lead to the development of low cholesterol eggs during late laying period in Hy-Line Brown hens.

• Journal of Animal Science and Technology
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• 제55권2호
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• pp.115-122
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• 2013

This study was conducted to evaluate the effect of dietary ${\beta}$-mannanase supplementation and palm kernel meal (PKM) inclusion (5%) on laying performance, egg quality and nutrient utilizability of laying hens with 73 weeks of age. A total of 240 Lohmann brown laying hens with average 77.5% egg production were randomly allocated with 60 hens per treatment, 4 replicates per treatment and 15 hens per replicate. Experimental design was a completely randomized design with $2{\times}2$ factorial arrangement, with the factors being (1) two levels of PKM (0 vs. 5%) and (2) with or without dietary ${\beta}$-mannanase (480 IU/kg of diet CTCzyme$^{(R)}$) supplementation. All hens were housed in cages ($35cmW{\times}35cmD{\times}40cmH$) with 2 hens per cage for six weeks feeding trial. Laying performance was recorded daily during feeding trial. Egg quality, nutrients utilizability and blood assays were done at the end of feeding trial. Egg production was improved (P<0.05) by both dietary PKM inclusion and ${\beta}$-mannanase combined supplementation. Either ${\beta}$-mannanase or PKM did not affect feed intakes and feed conversion ratio of all diets. Egg weight of hens fed diet containing 5% of PKM had heavier (P<0.05) eggs compared with hens fed without PKM. Albumen height was improved (P<0.05) by dietary mannanase supplementation. Crude fat utilization of 5% PKM diet was higher than that of no PKM diet regardless of ${\beta}$-mannanase supplementation. Both DM and total carbohydrate utilization were decreased (P<0.05) in hens fed 5% PKM diet. Serum IgG and yolk IgY contents of PKM groups were lower (P<0.05) than those of no PKM groups. This result showed that 5% PKM diet, independent of dietary ${\beta}$-mannanase supplementation, was able to improve egg production. In addition, dietary ${\beta}$-mannanase supplementation could be used for improving the albumen height of eggs.