• Biomolecules & Therapeutics
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• 제20권5호
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• pp.433-445
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• 2012

During the course of evolution, animals encountered the harmful effects of fungi, which are strong pathogens. Therefore, they have developed powerful mechanisms to protect themselves against these fungal invaders. ${\beta}$-Glucans are glucose polymers of a linear ${\beta}$(1,3)-glucan backbone with ${\beta}$(1,6)-linked side chains. The immunostimulatory and antitumor activities of ${\beta}$-glucans have been reported; however, their mechanisms have only begun to be elucidated. Fungal and particulate ${\beta}$-glucans, despite their large size, can be taken up by the M cells of Peyer's patches, and interact with macrophages or dendritic cells (DCs) and activate systemic immune responses to overcome the fungal infection. The sampled ${\beta}$-glucans function as pathogen-associated molecular patterns (PAMPs) and are recognized by pattern recognition receptors (PRRs) on innate immune cells. Dectin-1 receptor systems have been incorporated as the PRRs of ${\beta}$-glucans in the innate immune cells of higher animal systems, which function on the front line against fungal infection, and have been exploited in cancer treatments to enhance systemic immune function. Dectin-1 on macrophages and DCs performs dual functions: internalization of ${\beta}$-glucan-containing particles and transmittance of its signals into the nucleus. This review will depict in detail how the physicochemical nature of ${\beta}$-glucan contributes to its immunostimulating effect in hosts and the potential uses of ${\beta}$-glucan by elucidating the dectin-1 signal transduction pathway. The elucidation of ${\beta}$-glucan and its signaling pathway will undoubtedly open a new research area on its potential therapeutic applications, including as immunostimulants for antifungal and anti-cancer regimens.

• Archives of Plastic Surgery
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• 제39권4호
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• pp.323-328
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• 2012

Background Among the various methods for correcting nasal deformity, the composite graft is suitable for the inner and outer reconstruction of the nose in a single stage. In this article, we present our technique for reconstructing the ala and columella using the auricular chondrocutaneous composite graft. Methods From 2004 to 2011, 15 cases of alar and 2 cases of columellar reconstruction employing the chondrocutaneous composite graft were studied, all followed up for 3 to 24 months (average, 13.5 months). All of the patients were reviewed retrospectively for the demographics, graft size, selection of the donor site and outcomes including morbidity and complications. Results The reasons for the deformity were burn scar (n=7), traumatic scar (n=4), smallpox scar (n=4), basal cell carcinoma defect (n=1), and scar contracture (n=1) from implant induced infection. In 5 cases of nostril stricture and 6 cases of alar defect and notching, composite grafts from the helix were used ($8.9{\times}12.5$ mm). In 4 cases of retracted ala, grafts from the posterior surface of the concha were matched ($5{\times}15$ mm). For the reconstruction of the columella, we harvested the graft from the posterior scapha ($9{\times}13.5$ mm). Except one case with partial necrosis and delayed healing due to smoking, the grafts were successful in all of the cases and there was no deformity of the donor site. Conclusions An alar and columellar defect can be reconstructed successfully with a relatively large composite graft without donor site morbidity. The selection of the donor site should be individualized according to the 3-dimensional configuration of the defect.

• Archives of Plastic Surgery
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• 제36권2호
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• pp.167-173
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• 2009

Purpose: The key points of treatment of cryptotia are the elevation of invaginated ear helix and the correction of deformed cartilage. Prevention of stabilized cartilage contouring from returning to the previous state is also important. The authors carried otoplasty by modified Onizuka's method or Ohmori's method that conchal cartilage graft or high density polyethylene implant(MEDPOR$^{(R)}$) graft served as fixation after spreading posterior aspect of adhered antihelix and a splint for prevention of recurrence of cartilage deformities. The aim of this study is to reveal the availability of the high density polyethylene implant(MEDPOR$^{(R)}$) graft for the correction of cryptotia. Methods: We have repaired 17 cryptotic deformities using cartilage graft from cavum of concha(12 cases) or high density polyethylene implant(5 cases) for correction of deformed cartilage. We investigate the operative time, complications, and satisfaction of postoperative ear shape on both autogenous cartilage graft group and high density polyethylene implant graft group. Results: There was 1 case of reinvagination on autogenous cartilage graft group. Implant exposure was occurred on high density polyethylene implant graft group, as 1 case. These were statistically no differences between autogenous cartilage graft group and high density polyethylene implant graft group to the satisfaction of ear shape. Conclusion: High density polyethylene implant(MEDPOR$^{(R)}$) present an alternative to autogenous material as they allow of fibrovascular ingrowth, leading to stability of the implant and decreased infection rates. The correction of deformed cartilage by using the high density polyethylene implant(MEDPOR$^{(R)}$) is a good option for the treatment of cryptotia.

• Fibers and Polymers
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• 제7권2호
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• pp.129-138
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• 2006

Using a strain-controlled rheometer, the steady shear flow properties of aqueous xanthan gum solutions of different concentrations were measured over a wide range of shear rates. In this article, both the shear rate and concentration dependencies of steady shear flow behavior are reported from the experimentally obtained data. The viscous behavior is quantitatively discussed using a well-known power law type flow equation with a special emphasis on its importance in industrial processing and actual usage. In addition, several inelastic-viscoplastic flow models including a yield stress parameter are employed to make a quantitative evaluation of the steady shear flow behavior, and then the applicability of these models is also examined in detail. Finally, the elastic nature is explained with a brief comment on its practical significance. Main results obtained from this study can be summarized as follows: (1) Concentrated xanthan gum solutions exhibit a finite magnitude of yield stress. This may come from the fact that a large number of hydrogen bonds in the helix structure result in a stable configuration that can show a resistance to flow. (2) Concentrated xanthan gum solutions show a marked non-Newtonian shear-thinning behavior which is well described by a power law flow equation and may be interpreted in terms of the conformational status of the polymer molecules under the influence of shear flow. This rheological feature enhances sensory qualities in food, pharmaceutical, and cosmetic products and guarantees a high degree of mix ability, pumpability, and pourability during their processing and/or actual use. (3) The Herschel-Bulkley, Mizrahi-Berk, and Heinz-Casson models are all applicable and have equivalent ability to describe the steady shear flow behavior of concentrated xanthan gum solutions, whereas both the Bingham and Casson models do not give a good applicability. (4) Concentrated xanthan gum solutions exhibit a quite important elastic flow behavior which acts as a significant factor for many industrial applications such as food, pharmaceutical, and cosmetic manufacturing processes.

• 한국지반신소재학회논문집
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• 제16권4호
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• pp.1-11
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• 2017

본 연구에서는 헬리컬 파일의 관입시간과 극한하중에 관한 연구를 진행하고자 1/6 축소모형 실험을 진행하였다. 균질한 상대밀도의 사질토 지반을 형성하기 위하여 강사법을 사용하였다. 실험 조건으로 헬리컬 파일의 나선형 원판(heilx plate) 간격을 50mm와 150mm 두 Type으로 제작하였고, 헬리컬 파일의 회전관입 시 회전속도를 15rpm과 30rpm으로 진행하였다. 관입 완료된 헬리컬 파일에 콘 장비를 항타하여 관입하였다. 콘 장비 관입으로 헬리컬 파일의 극한하중 증가와 폐색의 정도를 확인 할 수 있었다. 위의 실험 결과를 토대로 헬리컬 파일의 관입시간은 "회전속도 30rpm-나선형 원판 간격 50mm"에서 단축 되었고, 극한하중은 "회전속도 30rpm-나선형 원판 간격 150mm-콘 장비 유"인 조건에서 높은 극한하중을 보였다.

• 한국전산구조공학회논문집
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• 제25권2호
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• pp.119-127
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• 2012

와이어 로프(wire rope)는 여러 가닥의 얇고 긴 철사를 감아서 밧줄과 같이 만든 것으로 굽힘과 비틀림 하중에 비해 축 하중에 더 크게 저항할 수 있는 특징을 가지고 있다. 해상 크레인, 갠트리 크레인, 크롤러 크레인 등과 같이 선박 또는 해양 구조물의 탑재를 위해 사용되는 조선용 탑재 크레인은 이러한 와이어 로프를 이용해 큰 중량의 블록들을 들거나 내리고 있다. 따라서 블록의 안전한 탑재를 위해서는 와이어 로프에 대한 동역학적 특성을 잘 파악해야 한다. 본 연구에서는 블록 탑재 시 크레인의 와이어 로프에 작용하는 장력과 비틀림 모멘트에 대한 계산식을 유도하고, 이를 기반으로 한 조선용 탑재 크레인의 동역학 시뮬레이션을 수행하였다. 그 결과, 개발된 시뮬레이션 방법이 실제 조선소의 안전한 블록 탑재 과정에 충분히 적용할 수 있음을 확인하였다.

• 한국결정학회:학술대회논문집
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• 한국결정학회 2002년도 정기총회 및 추계학술연구발표회
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• pp.4-4
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• 2002

PDZ domains bind to short segments within target proteins in a sequence-specific fashion. GRIP/ABP family proteins contain six to seven PDZ domains and interact via its sixth PDZ domain (class Ⅱ) with the C-termini of various proteins, including liprin-α. In addition the PDZ456 domain mediates the formation of homo- and heteromultimers of GRIP proteins. To better understand the structural basis of peptide recognition by a class Ⅱ PDZ domain and DZ-mediated multimerization, we determined the crystal structures of the GRIPI PDZ6 domain, alone and in complex with a synthetic C-terminal octapeptide of human liprin-α, at resolutions of 1.5 Å and 1.8 Å, respectively. Remarkably, unlike other class Ⅱ PDZ domains, Ile736 at αB5 rather than conserved Leu732 at αB1 makes a direct hydrophobic contact with the side chain of the Tyr at the -2 position of the ligand. Moreover, the peptide-bound structure of PDZ6 shows a slight reorientation of helix αB, indicating that the second hydrophobic pocket undergoes a conformational adaptation to accommodate the bulkiness of the Tyr's side chain, and forms an antiparallel dimer through an interface located at a site distal to the peptide-binding groove. This configuration may enable formation of GRIP multimers and efficient clustering of GRIP-binding proteins.

• Journal of the Korean Wood Science and Technology
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• 제15권2호
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• pp.79-89
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• 1987

This study was executed to examine helical structure and other feature, of Taxus cuspidata S. et Z. and Larix gmelinii var. principis-ruprechtii Pilger through photomicroscopic and scanning electron microscopic observation, and the obtained result, were summarized as follows: 1. Tracheid transition from springwood to summerwood was abrupt, mel slightly gradual in Taxus cuspidata S. et Z. and summerwood width of Larix gmelinii var. principis-ruprechtii Pilger was wider than that of Taxus cuspidata S. et Z. 2. Normal vertical and horizontal resin canals and surrounding thick epithelial cells were present in Larix gmelinii var. principis-ruprechtii Pilger but not present in Taxus cuspidata S. et Z. 3. Instead of helical thickening, helical chicks were observed only in summerwood trachieds of Larix gmelinii var. principis-ruprechtii Pilger. However, helical thickenings forming S, Z., and horizontal helix perpendicular to long axis of tracheid were observed in springood and summerwood tracheids of Taxus cuspidata S. et Z. and these helical thickenings were considered as analogous to the innermost layer of secondary wall. 4. Uniseriate and fusiform rays were appeared on tangential surface of Larix gmelinii var. principis-ruprechtii Pilger but oly uniserate ray was observed on tangential surface ot Taxus cuspidata S. et Z. The fusiform rays of Larix gmelinii var. principis-ruprechtii Pilger usually contained one horizontal resin canal bot occasionally two horizontal resin canals were contained in a fusiform ray. 5. Trabeculae and crassulae were observed in the tracheids of Larix gmelimii var. principis-ruprechtii Pilger but not observed in the tracheids of Taxus cuspidata S. et Z.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2003년도 정기총회 및 학술발표회
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• pp.34-34
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• 2003

Protein unfolding is a key step in several cellular processes, including protein translocation across some membranes and protein degradation by ATP-dependent proteases. C1pAP protease and the proteasome can actively unfold proteins in a process that hydrolyzes ATP, These proteases catalyze unfolding by processively unraveling their substrates from the attachment point of the degradation signal. As a consequence, the ability of a protein to be degraded depends on its structure as well as its stability. An ${\alpha}$-helix is easier to unravel than a ${\beta}$-strand. In multidomain proteins, independently stable domains are unfolded sequentially. The steric constraints imposed on substrate proteins during their degradation by the proteasome were investigated by constructing a model protein in which specific parts of the polypeptide chain were covalently connected through disulfide bridges. The cross-linked model proteins were fully degraded by the proteasome, but two or more cross-links retarded the degradation slightly. Our results suggest that the pore of the proteasome allows the concurrent passage of at least three stretches of a polypeptide chain, and also explain the limited degradation by the proteasome that occurs in the processing of the transcription factor NF-KB, and also implicate difficulty in degradation of amyloidal aggregates by the proteasome

• 한국경제지리학회지
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• 제13권4호
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• pp.601-622
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• 2010

본 연구는 대구문화콘텐츠산업 클러스터를 대상으로 가치사슬 변화가 지역경제에 미치는 영향을 분석하고 이에 대한 정책대안을 모색하고자 한다. 이를 위해 가치사슬의 부가가치 창출구조의 변화와 가치사슬의 변화에 영향을 미치는 제도적 환경과 혁신 주체들의 네트워크 변화를 분석하였다. 그 결과 대구문화콘텐츠산업 클러스터의 가치사슬은 직간접적 부가가치 창출 활동의 범위가 확대되었고 지역 내 혁신주체들과의 네트워크도 증가하였다. 이러한 가치사슬의 변화는 지역경제에 긍정적인 영향을 미치는 것으로 평가된다. 그러나 지역 문화콘텐츠 산업클러스터가 지속적인 경쟁력을 갖기 위해서는 기업의 역량 강화뿐만 아니라 문화콘텐츠산업을 둘러싼 제도적 문화적 환경의 창출과 혁신주체들 간의 트리플 힐릭스체제의 강화가 요구된다.