• International Journal of Oral Biology
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• v.39 no.4
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• pp.169-176
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• 2014

A positional scanning synthetic peptide combinatorial library (PS-SCL) was screened in order to identify antimicrobial peptides against the cariogenic oral bacteria, Streptococcus mutans. Activity against Streptococcus gordonii and Aggregatibacter actinomycetemcomitans was also examined. The library was comprised of six sub-libraries with the format $O_{(1-6)}XXXXX-NH_2$, where O represents one of 19 amino acids (excluding cysteine) and X represents equimolar mixture of these. Each sub-library was tested for antimicrobial activity against S. mutans and evaluated for antimicrobial activity against S. gordonii and A. actinomycetemcomitans. The effect of peptides was observed using transmission electron microscopy (TEM). Two semi-mixture peptides, RXXXXN-$NH_2$ (pep-1) and WXXXXN-$NH_2$ (pep-2), and one positioned peptide, RRRWRN-$NH_2$ (pep-3), were identified. Pep-1 and pep-2 showed significant antimicrobial activity against Gram positive bacteria (S. mutans and S. gordonii), but not against Gram negative bacteria (A. actinomycetemcomitans). However, pep-3 showed very low antimicrobial activity against all three bacteria. Pep-3 did not form an amphiphilic ${\alpha}$-helix, which is a required structure for most antimicrobial peptides. Pep-1 and pep-2 were able to disrupt the membrane of S. mutans. Small libraries of biochemically-constrained peptides can be used to generate antimicrobial peptides against S. mutans and other oral microbes. Peptides derived from such libraries may be candidate antimicrobial agents for the treatment of oral microorganisms.

• BMB Reports
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• v.43 no.11
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• pp.744-749
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• 2010

The aim of this work was to determine whether intrinsically bent DNA sites are present at, or close to, the mammalian replication origins oriGNAI3 and oriB in the Chinese hamster AMPD2 locus. Using an electrophoretic mobility shift assay and in silico analysis, we located four intrinsically bent DNA sites (b1 to b4) in a fragment that contains the oriGNAI3 and one site (b5) proximal to oriB. The helical parameters show that each bent DNA site is curved in a left-handed superhelical writhe. A 2D projection of 3D fragment trajectories revealed that oriGNAI3 is located in a relatively straight segment flanked by bent sites b1 and b2, which map in previously identified Scaffold/Matrix Attachment Region. Sites b3 and b4 are located approximately 2 kb downstream and force the fragment into a strong closed loop structure. The b5 site is also located in an S/MAR that is found just downstream of oriB.

• KSBB Journal
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• v.18 no.5
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• pp.352-355
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• 2003

${\beta}$-(1,6)-Branched ${\beta}$-(1,3)-D-glucans are known to enhance the immune system in human body, and in most cases have higher molecular weights over 1 MDa. In order to enhance the efficacy of glucans by decreasing their molecular weights, sonication, acid treatment, and enzymatic hydrolysis were tested and compared in this work. Treatment of sonication was effective to decrease the molecular weight to the extent of several dozens of kilo-daltons, but have a risk to disorder the triple helical structure of the glucans. Acid treatment was also an effective method to degrade polysaccharides, but ${\beta}$-(1,6)-branched of the glucan molecules was found to be also hydrolyzed. Treatment of ${\beta}$-(1,3)-glucanase was an effective method to decrease the molecular weight in mild conditions, but could not hydrolyse the highly ${\beta}$-(1,6)-branched ${\beta}$-(1,3)-glucans efficiently.

• Journal of the Korea Convergence Society
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• v.2 no.2
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• pp.27-34
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• 2011

This study is making a product for the development of one process worm wheel of making a shape for gear for worm wheel without hobbing manufacturing process. Because of removing a hobbing process, plastic worm wheel for increased productivity and equivalent quality is produced in the result. As the result, this product is selling to Hyundai Mobis, Mando, TRW, KOYO/NSK/Showa(Japan), Delphai(America). The core technology and different strategy are as follows. The technology protection for molding of worm whee is currently patent process "Molding process of helical gear(No. 10-2008-0105908). Further patent procedure for "molding system for positioning decision of inserting boss is currently prepared. As gear molding procedure in hobbing machine without gear machining procedure, most of all, core development technology which is making a gear tooth is main topic. So that, in case of currently developed worm wheel, because core and mold base are not developed in the first procedure, gear is machining in hobbing M/C as the second procedure. In the later, patent for mold base structure will be prepared in this study results.

• International Journal of Aeronautical and Space Sciences
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• v.6 no.2
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• pp.84-96
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• 2005

Helical tip vortex is known as stable vortex structure, however the specific frequency component of far wake perturbation induces the vortex pairing in hover and axial flight. It is expected that the tip vortex pairing phenomena may happen in transition flight and very low advance ratio flight so that inflow may be most nonuniform in the low advance ratio flight. The objectives of this paper are that a tip-vortex instability during the transition from hover into very low advance ratio forward flight is numerically predicted to understand a physics by using a time-marching free-wake method. To achieve the objectives, numerical method is firstly validated in typical axial and forward flights cases. Present scheme with trim routine can predict airloads and inflow distribution of forward flight with good accuracy. Then, the transition flight condition is calculated. The rotor used in this wake calculation is a small-scale AH-1G model. By using a tip-vortex trajectory tracking method, the tip-vortex pairing process are clearly observed in transient flight($\mu$=0.03) and disappears at a slightly higher advance ratio($\mu$=0.05). According to the steady flight simulation at $\mu$=0.03, it is confirmed the tip-vortex pairing process is continued in the rear part of rotor disk and not occurs in the front part. Time averaged inflow in this case is predicted as smooth distribution.

• Proceeding of EDISON Challenge
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• 2015.03a
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• pp.14-23
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• 2015

Alzheimer's disease is one of the most common types of degenerative dementia. As a considerable cause of Alzheimer's disease, neurotoxic plaques composed of 39 to 42 residue-long amyloid beta($A{\beta}$) fibrils have been found in the patient's brain in large quantity. A previous study found that erythrosine B (ER), a red color food dye approved by FDA, inhibits the formation of amyloid beta fibril structures. Here, in an attempt to elucidate the inhibition mechanism, we performed molecular dynamics simulations to demonstrate the conformational change of $A{\beta}40$ induced by 2 ERs in atomistic detail. During the simulation, the ERs bound to the surfaces of both N-terminus and C-terminus regions of $A{\beta}40$ rapidly. The observed stacking of the ERs and the aromatic side chains near the N-terminus region suggests a possible inhibition mechanism in which disturbing the inter-chain stacking of PHEs destabilizes beta-sheet enriched in amyloid beta fibrils. The bound ERs block water molecules and thereby help stabilizing alpha helical structure at the main chain of C-terminus and interrupt the formation of the salt-bridge ASP23-LYS28 at the same time. Our findings can help better understanding of the current and upcoming treatment studies for Alzheimer's disease by suggesting inhibition mechanism of ER on the conformational transition of $A{\beta}40$ at the molecular level.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.25 no.3
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• pp.237-243
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• 2012

The thickness optimization of the gearbox housing for 5MW wind turbine is carried out with the help of the efficient structure analysis model and the approximation model of objective function. Wind turbine gearbox is a complex structural system composed of a number of gear trains, shafts, bearing and gearbox housing, requiring a tremendous number of elements for the structural analysis and design. In this paper, an effective analysis and design model considering the tooth stiffness of helical gears is proposed. It enables to significantly reduce the total element number and the analysis time. Through the numerical optimization of housing thickness making use of the effective gearbox model and the approximate model of objective function, the total weight of the gearbox housing is minimized. It has been observed from the numerical experiment that the approximation model is reliable and the optimization result is acceptable and verified analysis.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.550-551
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• 2013

Large-area RF-driven ion source is being developed at Germany for the heating and current drive of ITER plasmas. Negative hydrogen (deuterium) ion sources are major components of neutral beam injection systems in future large-scale fusion experiments such as ITER and DEMO. RF ion sources for the production of positive hydrogen ions have been successfully developed at IPP (Max-Planck- Institute for Plasma Physics, Garching) for ASDEX-U and W7-AS neutral beam injection (NBI) systems. In recent, the first NBI system (NBI-1) has been developed successfully for the KSTAR. The first and second long-pulse ion sources (LPIS-1 and LPIS-2) of NBI-1 system consist of a magnetic bucket plasma generator with multi-pole cusp fields, filament heating structure, and a set of tetrode accelerators with circular apertures. There is a development plan of large-area RF ion source at KAERI to extract the positive ions, which can be used for the second NBI (NBI-2) system of KSTAR, and to extract the negative ions for future fusion devices such as ITER and K-DEMO. The large-area RF ion source consists of a driver region, including a helical antenna (6-turn copper tube with an outer diameter of 6 mm) and a discharge chamber (ceramic and/or quartz tubes with an inner diameter of 200 mm, a height of 150 mm, and a thickness of 8 mm), and an expansion region (magnetic bucket of prototype LPIS in the KAERI). RF power can be transferred up to 10 kW with a fixed frequency of 2 MHz through a matching circuit (auto- and manual-matching apparatus). Argon gas is commonly injected to the initial ignition of RF plasma discharge, and then hydrogen gas instead of argon gas is finally injected for the RF plasma sustainment. The uniformities of plasma density and electron temperature at the lowest area of expansion region (a distance of 300 mm from the driver region) are measured by using two electrostatic probes in the directions of short- and long-dimension of expansion region.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.68-68
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• 2003

HP(2-20) (AKKVFKRLEKLEKLFSKIQNDK) derived from the N-terminus of Helicobacter pylori Ribosomal Protein L1 shows potent antimicrobial activity against bacterial, fungi and cancer cells without cytotoxic effect. In order to investigate the relationships between antimicrobial activity and the structures, several analogues have been designed and synthesized. The structures of these peptides in SDS micelles have been investigated using NMR spectroscopy and they revealed that analogue 3 has the longest, well-defined alpha-helix from Val5 to Trp19. NOESY experiments performed on HP and its analogues in nondeuterated SDS micelles show that protons in the indole ring of Trp16 are in close contact with methylene protons of SDS micelles. In order to probe the position of HP and its analogues relative to the SDS micelles, spin-labeled stearate was added. Large effects are observed for the chemical shifts and the intensities of Phe5, Glu9, Phe12, and Trp16 within the helix region by 16-doxylstearate. This result implies that 16-doxylstearate is located in the center of the micelles and the hydrophobic phase of the amphiphilic ${\alpha}$-helix is located in contact with the acyl chains of the micelles. Also, Lys3 and Lys4 at N-terminus and Lys20 at C-terminus may produce an optimal arrangement for electrostatic interactions between the sulfate head groups of the SDS and the positively charged lysyl N$\sub$3/$\^$+/. Interactions between the indole ring of Trp and the membrane, as well as the amphiphilic ${\alpha}$-helical structure of HP induced by Trp at the C-terminus may allow HP to span the lipid bilayer. These structural features are crucial for their potent antibiotic activities.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.18 no.12
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• pp.2823-2830
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• 2014

The antenna miniaturization technique involves the increment of the electrical length of the resonator the variation of the physical appearance of the antenna. The most typical method of size reduction is designing helical antenna, meander antenna, and fractal antenna. Size reduction using fractal antenna is proposed in this paper. Fractal koch curve has been etched in microstrip patch antenna to downsize the antenna at ISM (Industrial Scientific and Medical) frequency band of 2.45 GHz koch curve microstrip patch antenna ha FR4 epoxy substrate with dielectric constant 4.7, loss tangent equal to 0.02 and dielectric high of 1.6 mm. The designed antenna is fabricated using etching process. The fabricated antenna has return loss of 2.45 GHz, VSWR of 1.1492, and impedance is matched to $46{\Omega}$.