• Journal of Navigation and Port Research
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• v.40 no.3
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• pp.147-157
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• 2016

The trend of globalization and the development of the communication-Information technology have led the organization of a complex supply chains which are more vulnerable to risks. The impact of risk on the supply chain can be adverse so importance of risk management on a supply chain has increased. In order to analyze the risk factors of transport system, this study described about the definition of transport risk and investigated the relationship between likelihood index and effect index of each risk factor. We identified risk factors on transport system and measured likelihood index and effect index of each risk factor. Finally, a numerical risk index, which is a value of total transport system, has been resulted by aggregating all indices. In addition, a case study using the proposed method has done on a heavy vehicle transport context with a transport company.

• Journal of Microbiology and Biotechnology
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• v.13 no.2
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• pp.256-262
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• 2003

To enhance the production of heavy chain immunoglobulin G (HC IgG) in the suspension cultures of transgenic tobacco cells (Nicotiana tabacum), the effects of adding various cytokinins (i.e., growth regulators) and organic nitrogen sources to culture media were investigated. Four different cytokinins including kinetin, isopentenyladenine (IPA), 6-benzylaminepurine (BA), and zeatin were tested with or without dichlorophenoxyacetic acid (2,4-D), which is a typical growth regulator supplemented in the standard Murashige and Skoog (MS) medium. The productivity of intracellular HC IgG was increased by 36 and $42\%$, compared to the control, especially when IPA (2 mg/l) or BA (0.2 mg/l) was added to the media in the presence of 2,4-D, respectively. In the study of organic nitrogen sources, addition of each casein hydrolysate and tryptone to the culture media at a final concentration of 0.01 and 1 g/l, respectively. increased the productivity or he IgG as much as 68 and $67\%$, respectively, in comparison with the control, which was is MS medium without supplementation of any organic nitrogen sources. This study shows that the optimization of media composition could offer significant improvements in the production of foreign proteins in the suspension cultures of transgenic plants.

• Journal of the Society of Naval Architects of Korea
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• v.57 no.1
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• pp.45-51
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• 2020

In this study, we developed an allocation optimization system for supply chain management in the shipbuilding and offshore construction industry. Supply chain operation is a way of operating manufacturing company responsible for the procurement of outfitting parts. The method about how to allocate the manufacturing volume to each partner company includes important decisions. According to the allocation method, the stability of the material supplied to the final installation process is guaranteed. We improved the allocation method that was previously decided by the person in charge. Based on the optimization engine, a system is developed that can automatically allocate the production volume. For optimization model configuration, factors affecting the volume allocation were analyzed and modeled as constraint factors. A target function is defined to minimize the difference in the load variance of each partner company. In order to use the same type of volume allocation engine for various outfitting products, the amount of work done by the partner company was standardized. We developed an engine that can allocate the same production load of each production partner. Using this engine, the operating system was developed and applied to the actual offshore project. It has been confirmed that the work load variance of suppliers can be maintained uniformly using the optimization engine rather than manual method. By this system, we stabilize the manufacturing process of partner suppliers.

• The Journal of Internal Korean Medicine
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• v.39 no.6
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• pp.1168-1180
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• 2018

Objective: As the number of sarcopenic patients worldwide is increasing, the need for the treatment of sarcopenia is increasing. Ginseng has been reported to be a major herbal supplement. We tested whether red ginseng would be effective for sarcopenia using red ginseng preparation which can be easily obtained locally in Korea. Methods: 30 rats were randomly divided into three groups: the control group (n=10) (Group C), the group with Dexamethasone -induced sarcopenia (n=10) (Group D), and the group to which red ginseng was administered group after induced sarcopenia with Dexamethasone (n=10) (Group DH). Dexamethasone was intraperitoneally administered to group D and group DH for 7 days to make sarcopenic model. After that, the red ginseng tablets prepared by Korea Ginseng Corporation were diluted in distilled water and administered orally to the DH group for 2 weeks. Body weight and grip strength were measured 8 times during the experiment. At the end of the experiment, blood was collected by cardiac puncture. In addition, the tibialis muscle was extracted, a myofibril cross section was measured by immunohistochemical staining and MyHC (myosin heavy chain) was quantified by Western blotting. Results: The ratio of the area on myofibril cross-section showed significant differences after administration of the red ginseng tablet. Conclusions: Red ginseng has a significant effect on the recovery of myofibril cross-section on sarcopenia. This experiment will be helpful for future clinical studies on drug effects in sarcopennia.

• Journal of Animal Science and Technology
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• v.62 no.6
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• pp.893-902
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• 2020

This study was conducted to compare the mRNA expression levels of myogenic-adipogenic makers in the skeletal muscle and adipocytes formation, body weight, rumen weight, and papilla length on Hanwoo calves at newborn and 6 months of age. Animals used three newborn Hanwoo calves (NC) and three Hanwoo calves 6 months of age (SC). Body weight and rumen weight were significantly increased in SC compared to NC (p < 0.01), and papilla length was longer about 10-fold in SC than NC. Adipocytes was possible to visually identify more adipocytes in SC compared to NC, and were mainly formed around the blood vessels. mRNA expression of myogenin, myosin heavy chain 1 and myosin heavy chain 2A in both longissimus dorsi (LD) and semimembranosus (SM) was found to increase with calves growth (p < 0.01), and it was confirmed that have higher levels of mRNA expression in SM than LD. In LD tissues, the mRNA expression of stearoyl-CoA desaturase (SCD, p < 0.03) and peroxisome proliferator activated receptor γ (PPARγ, p < 0.04) was significantly higher in SC than NC. In SM tissues, mRNA expression levels of SCD (p < 0.02) and CCAAT/enhancer binding protein β (C/EBPβ, p < 0.01) were higher in SC than NC, and also mRNA expression levels of PPARγ increased, but there was no significant difference. Thus, the calves period suggests that it is an important step in the development of the rumen and the myogenesis and adipogenesis.

• Journal of The Korean Society of Agricultural Engineers
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• v.63 no.2
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• pp.75-84
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• 2021

As the weather changes become frequent, weather disasters are increasing, causing more damage to plastic greenhouses. Among the damage caused by various disasters, damage by snow to the greenhouse takes a relatively long time, so if an alarm system is properly prepared, the damage can be reduced. Existing greenhouse design standards and snow warning systems are based on snow depth. However, even in the same depth, the load on the greenhouse varies depending on meteorological characteristics and snow density. Therefore, this study aims to secure the structural safety of greenhouses by developing sensors that can directly measure snow loads, and analysing the warning criteria for load using a stochastic model. Markov chain was applied to estimate the failure probability of various types of greenhouses in various regions, which let users actively cope with heavy snowfall by selecting an appropriate time to respond. Although it was hard to predict the precise snow depth or amounts, it could successfully assess the risk of structures by directly detecting the snow load using the developed sensor.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.58 no.3
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• pp.230-240
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• 2022

In general, a high tension on the anchor and chain is placed when a ship at anchor is subjected to heavy weather. Mariners have to pay attention to whether dragging anchor occurs to keep the safety of the ship at anchorage since it is difficult to maintain the stable motion of ship and it causes collisions with other ships nearby. In this paper, the ship motion against the external forces was shown to obtain the fundamental data about characteristic of holding power due to nature of seabed at anchor, so practical trials were carried out in rocky area and muddy area using a trial ship around coastal area of South Korea. In muddy seabed, holding power showed reasonable tension values depending on the distance from anchor position of continuing swing motions of a ship corresponding to wind force. Meanwhile in rocky seabed, tension values on the chain appeared very high occasionally regardless of the distance from the anchor position and seemed to exceed its holding power to be the breaking strain of the chain although weather was not in a severe condition. Therefore, some of the cables laid on the seabed were presumed to be caught in a crack on the rock. It is assumed that even a small amount of external force may cause the chain to break in a moment in rocky seabed. Additionally, wind and current forces had a somewhat contradictory effect on holding power of the ship between them.

• Journal of Life Science
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• v.26 no.6
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• pp.698-704
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• 2016

The intracellular transport of organelles and protein complexes is mediated by kinesin superfamily proteins (KIFs). The first kinesin, kinesin 1, was identified as a molecular motor protein that moves various organelles and protein complexes along the microtubule rails in cells. Kinesin 1 is a tetramer of two heavy chains (KHCs, also called KIF5s) and two kinesin light chains (KLCs). KIF5s interact with many different proteins through their tail region, but their binding proteins have not yet been fully identified. To identify the interaction proteins for KIF5A, we performed yeast two-hybrid screening and found a specific interaction with ferritin heavy chain (Frt-h), which has a role in iron storage and detoxification. Frt-h bound to the amino acid residues between 800 and 940 of KIF5A and to other KIF5s in the yeast two-hybrid assay. The coiled-coil domain of Frt-h is essential for interaction with KIF5A. In addition, ferritin light chain (Frt-l) interacted with KIF5s in the yeast two-hybrid assay. In addition, these proteins showed specific interactions in the glutathione S-transferase (GST) pull-down assay. An antibody to KHC specifically co-immunoprecipitated Frt-h and Frt-l from mouse brain extracts. These results suggest the kinesin 1 motor protein may transport the ferritin complex in cells.

• KSBB Journal
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• v.21 no.3
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• pp.204-211
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• 2006

For the production of the recombinant human interferon-gamma(rhIFN-${\gamma}$) in Escherichia coli, human glucagon and ferritin heavy chain were used as fusion partners. Even though rhIFN-${\gamma}$ is expressed as an inclusion body form in E. coli because of strong hydrophobicity of itself, over 50% of fused rhIFN-${\gamma}$ was expressed as soluble form in E. coli $Origami^{TM}$(DE3) harboring pT7FH(HE)-IFN-${\gamma}$ which encodes ferritin heavy chain-fused rhIFN-${\gamma}$. In the case of using glucagon-ferritin heavy chain hybrid mutant as a fusion partner, 6X His-tag was additionally introduced to N-terminus of GFHM(HE)-IFN-${\gamma}$ for enhancing purification yields of rhIFN-${\gamma}$. Fusion protein HGFHM(HE)-IFN-${\gamma}$ with two 6X His-tag was more effectively bound to Ni-NTA agarose bead than GFHM(HE)-IFN-${\gamma}$ with a 6X His-tag. rhIFN-${\gamma}$ was completely purified from enterokinase-treated HGFHM(HE)-IFN-${\gamma}$ by Ni-NTA affinity column. For high-level production of rhIFN-${\gamma}$, glucose was used as the sole carbon source with simple exponential feeding rate($2.4{\sim}7.2g/h$) in fed-batch process. The effective lactose concentration for the expression of the rhIFN-${\gamma}$ was $10{\sim}20mM$. Under the fed-batch culture conditions, rhIFN-${\gamma}$ production yield reached 11 g DCW/L for 6 hours after lactose induction.

• Korean Journal of Food Science and Technology
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• v.50 no.2
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• pp.138-142
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• 2018

The physical properties and heavy metal content in rices that were grown in different conditions were compared; two paddy rices grown in irrigated water and a Jeju rice grown in dry field without irrigation. The pasting properties, color, or amylopectin chain length distribution were different, however, they were independent of growing conditions. For heavy metals such as Pb or Cd, Jeju rice showed similar content to paddy rices. However, total and inorganic arsenic (As) contents were much lower; for Jeju rice, the total arsenic content was only 10.1-17.9% of that in two paddy rices, and the inorganic arsenic was just corresponding to 15% of that in paddy rice. The inorganic arsenic content decreased by 34.1, 23.8, and 17.5% in paddy rice by soaking, cooking, and dripping with hot water, respectively.