• Journal of Microbiology and Biotechnology
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• v.17 no.1
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• pp.29-36
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• 2007

The xynA gene encoding the xylanase A of Paenibacillus sp. DG-22 was isolated with a DNA probe obtained by PCR amplification, using degenerated primers deduced from the amino acid residues of the known N-terminal region of the purified enzyme and the conserved region in the family 11 xylanases. The positive clones were screened on the LB agar plates supplemented with xylan, by the Congo-red staining method. The xynA gene consists of a 630-bp open reading frame encoding a protein of 210 amino acids, and the XynA preprotein contains a 28-residues signal peptide whose cleavage yields a l82-residues mature protein of a calculated molecular weight of 20,000Da and pI value of 8.77. The cloned DNA fragment also has another ORF of 873 nucleotides that showed 76% identity to the putative transcriptional activator of Bacillus halodurans C-125. Most of the xylanase activity was found in the periplasmic space of E. coli. The xynA gene was subcloned into pQE60 expression vector to fuse with six histidine-tag. The recombinant xylanase A was purified by heating and immobilized metal affinity chromatography. The optimum pH and temperature of the purified enzyme were 6.0 and $60^{\circ}C$, respectively. This histidine-tagged xylanase A was less thermostable than the native enzyme.

• Transactions of the Korean Society of Automotive Engineers
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• v.7 no.6
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• pp.123-134
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• 1999

Nowadays since the automobile is regarded as the third living space, comfortable conditions are required in the passenger compartment. The customer's concern on air-conditioning/heating, ventilation and demisting/defrosting performances has been much increased. Both ventilation and demisting /defrosting performances are directly influenced by register location, shape of regist guide vane, ventilation flow rate, air distribution , and air circulation pattern. Diffuse plume of air from the register is desirable not only to maintain comfort when the comfort when the comfortable condition has been satisfied but to improve demisting /defrosting performance. In this study, experimental and numerical investigation about the flow field of six different register vane types were carried out , respectively. The numerical analysis, based upon the $textsc{k}$-$\varepsilon$ turbulence model , was applied to the air flow field. The results show that the shape of register guide vane should be considered as an important design paramter.

• 한국연소학회:학술대회논문집
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• 2005.10a
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• pp.58-63
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• 2005

As fuel cells approach commercialization, hydrogen production becomes a critical step in the overall energy conversion pathway. Reforming is a process that produces a hydrogen-rich gas from hydrocarbon fuels. Hydrogen production via autothermal reforming (ATR) is particularly attractive for applications that demand a quick start-up and response time in a compact size. However, further research is required to optimize the performance of autothermal reformers and accurate models of reactor performance must be developed and validated. The design includes the requirement of accommodating a wide range of experimental set ups. Factors considered in the design of the reformer are capability to use multiple fuels, ability to vary stoichiometry, precise temperature and pressure control, implementation of enhancement methods, capability to implement variable catalyst positions and catalyst arrangement, ability to monitor and change reactant mixing, and proper implementation of data acquisition. A model of the system was first developed in order to calculate flowrates, heating, space velocity, and other important parameters needed to select the hardware that comprises the reformer. Predicted performance will be compared to actual data once the reformer construction is completed. This comparison will quantify the accuracy of the model and should point to areas where further model development is required. The end result will be a research tool that allows engineers to optimize hydrogen production via autothermal reformation.

• Journal of the Korean Institute of Rural Architecture
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• v.14 no.1
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• pp.9-19
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• 2012

The object of this study is to investigate the satisfaction level of group homes for the elderly living alone in rural areas. Sixty-four elderly people, living alone, were selected from ten regions operated by the Gimje city council in Jeonlabuk-do, and then a survey was conducted during January 17 to 26 in 2011. The results of the investigation are as follows: first, roughly 90.6 percent of the participants responded that they were satisfied with cohabitation. Second, average point of satisfaction was going to bed together, which is 4.39, also each unit in all the ten regions was recorded above 4.00. Third, the satisfaction of eating together averaged highly at 4.37. Fourth, 95 percent of them were satisfied with the conditions of the residential space. Fifth, the satisfaction with the amenities, such as electricity, phone, heating and sanitation, averaged 96.9 and 98.4 percent each, which was rated highest. Through analytical research, elderly people who live in 'group homes' are satisfied with their lives. It is thought that this 'group home' can play the role of an alternative family better than existing 'senior citizen centers' and 'welfare centers for the elderly'. The study means that 'group homes' in rural areas could be established successfully.

• KIEAE Journal
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• v.15 no.2
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• pp.117-122
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• 2015

Purpose: Generally, 30% of the total energy consumption in office building is used for artificial indoor lightings, and almost 75-85% of electric power in fluorescent and Light-Emitting Diode (LED) lightings can be dissipated as a form of heat into indoor environment. The heat generated by indoor lightings can cause the increase of cooling load in office buildings. Thus, it its important to consider indoor lightings as a heat and light source, simultaneously. Method: In this study, we installed two kinds of indoor lightings including fluorescent and LED lightings and measured surface temperature of both indoor lightings. In addition, we obtained ambient temperature of indoor space and finally calculated total heat dissipated from plenum area and surface of lightings. Result: Total indoor heat gain was 87.17Wh and 201.36Wh in cases of six 40W-LED lightings and 64W-fluorescent lightings, respectively.

• 한국태양에너지학회:학술대회논문집
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• 2009.04a
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• pp.83-90
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• 2009

As a series of 'Performance Assessment of Building Envelopes I: Double Skin Facade', three types (interior, exterior, mixed (int.&ext.)) of lightshelves and RetroLux were examined in terms of $CO_2$ emissions. It is shown that the exterior lightshelf could achieve the most energy savings (9.6-38.7%) in general office buildings due to blocking solar radiation before entering the indoor space. However, the interior lightshelf is the worst (1.4-5.2%) among three of them. The RetroLux has two components: (1) sun-reflector (first louver component), (2) light shelf for improving daylight induction (second louver component). Due to these two components, solar radiation from windows is filtered depending on seasonal variation (solar altitude). Therefore, the RetroLux can reduce 18.0-27.9% of annual energy consumption (both cooling and heating), and $552-3,290Won/m^2{\cdot}yr$ of operation cost is saved.

• 한국태양에너지학회:학술대회논문집
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• 2008.04a
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• pp.104-110
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• 2008

This paper presents demonstration study results derived through field testing of a part load solar energized cooling system for the library of a cultural center building located in Gwangju, Korea. First operating demonstration system was set up in Gwangju in 2005. These system comprises the $200m^2$ evacuated tubular solar collector, a $6m^3$ heat storage tank. In a 2006, daily average of insolation showed about $506W/m^2$, the solar collector efficiency was 44%. In a 2007, daily average of insolation showed about$507W/m^2$, the solar collector efficiency was 42%. As a result, evacuated tubular solar collector kept the high efficiency for two years.

• 한국태양에너지학회:학술대회논문집
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• 2008.04a
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• pp.184-189
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• 2008

These days, Apartment is altered oversized and high-rise through construction techniques development. Also, the cost of cooling and heating load would be expected by balcony expansion into inner space caused by legalization. In specially, HVAC systems of high-rise residential building almost apply to pre-existence techniques of middle and low residential building except for consideration and methods of the air inflow minimization into indoor through mechanical ventilation is used in ventilation system. From these cause, window systems of high-rise apartments stands high in estimation of components. Thus, purpose of this study is to make a comparative study through difference of window systems.

• KIEAE Journal
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• v.14 no.6
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• pp.105-110
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• 2014

The basic factors determining the amount of energy used in hospital buildings are weather conditions and building factors. But the real energy consumer is central plant equipment such as boilers and chillers that produce thermal energy for heating and cooling. Inaccurate decision of the primary equipment's size can cause a high initial-cost, an excessive equipment space, a wasted energy by low operation-efficiency and shortening of the machine's life. In this reason, the decision of optimal size for central plant equipment is very important. There are several factors for the decision such as an operation factor, a factor (equipment factor), piping losses and a simultaneous usage factor applied in the sizing process except a basic cooling load. But there is no standard method for applying those factors. Usually, factors are applied individually by an experience or custom of each engineer. In this study, the authors emphasize the meaning and the problem of those factors, examine them by analyzing factors which were applied to actual practices, and propose the recommendation value of safety, load, operation factors and application methods.

• KIEAE Journal
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• v.7 no.4
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• pp.57-62
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• 2007

At the moment, the reduction of building energy consumption is a unavoidable task of mankind for conserving global environment. Decreasing overall U-value of building envelope and air infiltration, especially in Korean climate condition with clear four seasons, are the obvious solutions for the objective. Thus low glazing ratio with small window openings are required for heating and cooling load reduction in buildings. Using larger window openings could provide better natural ventilation but it also increases the direct solar radiation penetration into indoor space, heat gain in summer and heat loss in winter. On the other hand, the ventilation rates decreasing problem with smaller window openings could be occurred. As a solution for it, the use of casement window can cause increasing natural ventilation rates by wing wall effect. This paper focuses on deduce the most efficient opening type of casement window in Korean climate. To estimate ventilation performance of each opening types, CFD simulation was used. The best performance of opening type in every wind direction is opening both windows to the center and the most appropriate opening type for Korean climate is also opening both windows to center.