• Title/Summary/Keyword: heat and irradiation

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Effect of Far-Infrared Irradiation and Heat Treatment on the Antioxidant Activity of Extracts from Citrus Pomaces (감귤박 추출물의 항산화능에 대한 원적외선과 열처리 효과)

  • Kim, Jong-Wan;Jeon, You-Jin;Lee, Jonh-Hwa;Lee, Seung-Cheol
    • Applied Biological Chemistry
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    • v.49 no.1
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    • pp.60-64
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    • 2006
  • The effect of far-infrared (FIR) irradiation and heat treatment on the antioxidant activity of extracts from citrus pomaces (CP) was evaluated. CP were placed in pyrex petri dishes (8.0 cm diameter) and irradiated at $100^{\circ}C$ and $150^{\circ}C$ for 5, 10, 15, 20, 40 or 60 min with a FIR heater or simple heater, respectively. After FIR irradiation or simple heat treatment, 70% ethanol extracts (EE) and water extracts (WE) of CP were prepared and radical scavenging activity (RSA) and reducing power of the extracts were determined. The antioxidant activities of the extracts increased as the time of heating or FIR-irradiation increased. When CP were FIR-irradiated at $100^{\circ}C$ far 30min, the values of RSA and reducing power of EE increased from 14.9% to 44.2%, and 0.290 to 0.886, respectively, compared to the untreated control. Simple heat treatment of CP at $100^{\circ}C$ for 60 min also increased RSA and reducing power of EE to 44.7%, and 1.045, respectively. FIR irradiation and heat treatment increased RSA and reducing power of WE from CP, too. These results indicated the antioxidant activity of CP extracts was significantly affected by FIR irradiation and heating temperature and duration on CP, and that the FIR irradiation and heat treatment process can be used as a tool for increasing the antioxidant activity of CP.

Effects of Sterilizing Methods on the Content of Index .Constituents of Herbal Medicines (품질 보증을 위한 멸균법이 수종 생약의 지표성분 함량에 미치는 영향)

  • Jeong, Choon-Sik;Cho, So-Yean;Lee, Yong-Soo
    • YAKHAK HOEJI
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    • v.50 no.6
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    • pp.415-420
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    • 2006
  • The purpose of this study was to develop the best methods to sterilize herbal medicines which is frequently used and known to have high susceptibility to microbial contamination. We used dry heat, gamma irradiation and alcohol gas treatment for sterilization, and evaluated these methods in terms of the followings; i) the efficacy of sterilization, and ii) the chemical alteration of index constituents of herbal medicines. Treatment with dry heat effectively eliminated the contaminated microorganisms, and did not significantly alter the content of berberine chloride, paeoniflorin and amygdalin in Phellodendron Bark, Peony Root and Apricot Kernel, respectively: However it seriously changed the color and morphology which are essential criteria to estimate a measure of quality of herbal medicines. Treatment with gamma irradiation showed a strong sterilizing effect, and no alteration of the content of index constituents, color and morphology: Alcohol gas treatment resulted in similar effects as those in gamma irradiation. Collectively; these results suggest that treatment with gamma irradiation or alcohol gas may be useful methods for sterilizing herbal medicines without a decrease in their microbial quality.

Study on the Chemical and Radiation Crosslinking of Poly(vinyl alcohol) Hydrogels for an Improvement of Heat Resistance (내열특성 개선을 위한 폴리(비닐 알코올) 수화젤의 화학 가교와 방사선 가교에 관한 연구)

  • Park, Kyoung-Ran;Nho, Young-Chang
    • Polymer(Korea)
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    • v.29 no.1
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    • pp.91-95
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    • 2005
  • The PVA hydrogels were prepared by the chemical and irradiation method to improve the heat resistance of these hydrogels at the high temperature. The physical properties such as the gel content, the degree of swelling and the gel strength for the synthesized hydrogels were examined. Gel content increased as the chemical reaction time and the irradiation dose increased, and gel content of the hydrogels were higher when the two-steps of chemical and irradiation method were used rather than only the chemical method. Gel strength increased as the chemical reaction time increased, and as the irradiation dose decreased. The hydrogels prepared by the chemical reaction for 5 hours and the two-steps had the heat resistance at the high temperature.

The Combined Effect of Heat Treatment and Irradiation on the Inactivation of Major Lactic Acid Bacteria Associated with Kimchi Fermentation (김치의 숙성관련 주요 젖산균 살균에 대한 가열처리와 방사선 조사의 병용효과)

  • Byun, Myung-Woo;Cha, Bo-Sook;Kwon, Joong-Ho;Cho, Han-Ok;Kim, Woo-Jung
    • Korean Journal of Food Science and Technology
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    • v.21 no.2
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    • pp.185-191
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    • 1989
  • The combined effects of heat treatment and ${\gamma}-irradiation$ on the inactivation of major lactic acid bacteria associated with Kimchi fermentation were investigated. The radiosensitivities $(D_{10}\;values)$ of lactic acid bacteria in case of a single treatment of irradiation were 0.61 kGy in Lactobacillus brevis, 0.60 kGy in Lactobacillus plantarum, 0.50 kGy in Leuconostoc mesenteroides, 0.4 kGy in Pediococcus cerevisiae, 0.39 kGy in Streptococcus faecalis. The heat sensitization $(D_{min}\;values)$ by a single treatment of heat ranged 9.2-15.6 at $50^{\circ}C$ and 3.7-5.5 at $60^{\circ}C$. Synegistic effects were shown in the radiosensitivities of Streptococcus faecalis, Pediococcus cerevisiae, Lactobacillus plantarum, and Lactobacillus brevis by the combined treatment(Dose multiplying factors ranged $1.20{\sim}1.56$). It seems, therefore, that the combined treatment can be applied to the radiation preservation of Kimchi, minimizing the side-effects like physical changes induced by the high dose irradiation or heat treatment.

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Improved Detection of ${\gamma}-Irradiated$ Vibrio vulnificus after Heat and Cold Shock Treatment by Using Ethidium Monoazide Real-time PCR

  • Lee, Jung-Lim;Levin, Robert E.
    • Food Science and Biotechnology
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    • v.18 no.3
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    • pp.788-792
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    • 2009
  • Gamma $({\gamma})-irradiation$ can be used to control pathogens such as Vibrio vulnificus in seafood. The effects of irradiation on microbial cell populations (%) have been studied in order to develop detection methods for irradiated foods. The method used in this study was ethidium bromide monoazide (EMA) real-time polymerase chain reaction (PCR), using V. vulnificus specific primer, EMA, and $SYBR^{(R)}$ Green to discriminate between ${\gamma}-irradiated$ and non-irradiated cells. Confocal microscope examination showed that ${\gamma}-irradiation$ damaged portions of the cell membrane, allowing EMA to penetrate cells of irradidated V. vulnificus. ${\gamma}-Irradiation$ at 1.08 KGy resulted in log reduction ($-1.15{\pm}0.13$ log reduction) in genomic targets derived from EMA real-time PCR. The combination cold/heat shock resulted in the highest ($-1.74{\pm}0.1$ log reduction) discrimination of dead irradiated V. vulnificus by EMA real-time PCR.

Inactivation of Spore-Forming Bacteria by Gamma Irradiation (감마선 조사에 의한 유포자 세균의 불활성화)

  • 변명우;권오진;육홍선
    • Journal of Food Hygiene and Safety
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    • v.11 no.3
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    • pp.197-201
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    • 1996
  • D10 values obtained for radiation alone in Bacillus subtilis and Clostridium perfrigenes were 0.35-0.48 kGy in vegetative cells, and 2~2.08 kGy in spores, respectively. Irradiation dose of 24 kGy completely inhibited spores. In the case of heat treatment, D50, 60 values ranged from 10 to 14 minutes in vegetative cells, and D70, 80, 90 values ranged from 10 to 140 minutes in spores. In the case of combined treatment with heat and radiation, D10 values ranged form 1 to 1.25 kGy in vegetative cells, and from 3.42 to 3.61 kGy in spores. Thus, resistance of cells to gamma radiation did not seem to be influences by pre-heating.

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Effects of Annealing and Neutron Irradiation on Micostructural and Mechanical Properties of High Burn-up Zr Claddings (고연소도 신형 Zr피복관의 미세조직과 기계적 특성에 미치는 열처리 및 중성자 조사의 영향)

  • Baek, Jong Hyuk;Kim, Hyun Gil;Jeong, Yong Hwan
    • Journal of the Korean Society for Heat Treatment
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    • v.17 no.3
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    • pp.151-164
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    • 2004
  • The changes of microstructural and mechanical properties were evaluated for the high burn-up fuel claddings after the neutron irradiation of $1.8{\sim}3.1{\times}10^{20}n/cm^2$ (E>1.0 MEV) in HANARO research reactor. After the irradiation, the spot-type dislocations (a-type dislocations) were easily observed in most claddings, and the density of the dislocations was different depending on the grains and was higher at grain boundaries than within grains. As the final annealing temperature increased, the density of spot-type dislocations increased and the line-type dislocations (c-type dislocations) which was perpendicular to the <0002> direction, appeared sporadically in some claddings. However, the types of precipitates in the fuel claddings after the irradiation were not changed from that in unirradiated claddings. The mechanical properties including the hardness, strength and elongation after the irradiation were changed due to the formation of spot-type dislocations. That is, the increase in hardness and strength as well as the decrease in elongation after the irradiation was occurred simultaneously with increasing the final annealing temperature. Owing to the Nb contribution to the formation of spot-type dislocation during the irradiation, the increase in hardness and strength in higher Nb-contained Zr alloys after the irradiation was higher than that in lower Nb-contained Zr alloys.

The Cold Function Test of a Main Cooling Water System for a Nuclear Fuel Test Loop Installed in HANARO (하나로 핵연료 시험장치의 주냉각수 계통 상온기능시험)

  • Park, Young-Chul;Lee, Young-Sub;Chi, Dai-Yong
    • Proceedings of the KSME Conference
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    • 2008.11b
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    • pp.2505-2510
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    • 2008
  • A nuclear fuel test loop (after below, FTL) is installed in IR1 of an irradiation hole in HANARO for testing neutron irradiation characteristics and thermo hydraulic characteristics of a fuel loaded in a light water power reactor or a heavy water power reactor. When HANARO is normally operated, the fuel loaded in the irradiation hole has a nuclear reaction heat generated by a neutron irradiation. To remove the generated heat and to maintain an operation condition of the test fuel, a main cooling water system (MCWS) is installed in the OPS of the FTL. This paper describes the cold function test results of the MCWS. It was confirmed through the test results that the system met the design requirements under a cold operation condition.

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Preparation and Characterization of $Ge_{20}As_{20}Se_{60}$ Amorphous Chalcogenide Thin Film by Spin Coating (Spin-coating에 의한 $Ge_{20}As_{20}Se_{60}$ 비정질 chalcogenide 박막의 제조 및 광특성 분석)

  • 이강구;최세영
    • Journal of the Korean Ceramic Society
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    • v.37 no.3
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    • pp.219-226
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    • 2000
  • Amorphous Ge20As20Se60 chalcogenide thin films were prepared by spin coating technique from mixed solutions of As40Se60 and Ge40Se60 dissolved in ethylenediamine. Films were prepared at a roating speed of 3500 rpm and spinning time was 10 second and heat-treateed at 27$0^{\circ}C$ for 1 hour. The resulting film thickness and RMS roughness were approximately 340 nm and 15$\AA$. Photostructure changes were investigated with 514.5nm Ar+ laser irradiation and heat-treatment. After Ar+ laser irradiation, transmittance and transmission efficiency decreased respectively up to 24.9% at 2.43 eV and 67.5% at 3.27 eV, and absorption edge shifted toward long wavelength. Optical bandgap changed from 2.03 to 1.83 eV, and absoprtion coefficient and absorption efficiency increased up to 0.33$\times$105cm-1 at 3.37eV and 88.3% at 1.31 eV, respectively. These photodarkening state were recovered reversibly by heat-treatment at 27$0^{\circ}C$ for 1 hour. Photodarkening and thermal bleaching effects by laser irradiation and heat-treatment revealed reversible amorphous-to-amorphous transition varying only coordination number.

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Inhibitory Effects of Tannic Acid on the Skin Toxicity and Heat Shock Protein Induction by UVB Irradiation in Hairless Mouse (자외선 B 파로 유도된 Hairless Mouse에서 타닌의 피부 독성 억제효과 및 Heat Shock Protein 70의 생성억제 효과)

  • 이세윤;이민경;장동덕;안령미;안형수
    • Toxicological Research
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    • v.13 no.1_2
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    • pp.79-86
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    • 1997
  • Inhibitory effects of tannic acid on skin toxicity and heat shock protein induced by UVB were investigated. Tannic acid was administered either topically or orally for 3 days to hairless mice, which were previously irradiated with UVB. UVB was found to cause skin erythema . However, the skin erythema was decreased when tannic acid was administered either topically or orally. The heat shock proteins, Hsp-78 kDa and 70 kDa, were induced by UVB irradiation, but the induction was decreased by treatment of tannic acid in both topically and orally administered groups. The hsp induction was more prominent in orally administered groups than in topically administerd groups. However, the difference between two groups was not statistically significant. The route of administrations, topical and oral, does not affect the activity of tannic acid. In the skin tissue observation, tannic acid regenerated the epithelial cells with 7-9 cell layers which were injured by UVB. In conclusion, tannic acid has an ability to protect against UVB irradiation and regenerate the skin.

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