• 대한의생명과학회지
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• 제21권4호
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• pp.227-232
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• 2015

Epstein-Barr virus (EBV) has a pathogenic role in several lymphomas including diffuse large B-cell lymphoma (DLBCL). In this study, we detected EBV from formalin-fixed paraffin embedded (FFPE) tissues of DLBCL patients by RT-PCR and compared the sensitivity of the RT-PCR method to in situ hybridization (ISH) method. The RNA was extracted from 91 FFPE samples with DLBCL and amplified with primers targeting EBV-encoded small RNA (EBER) by RT-PCR. When using the RT-PCR method, 13 of 91 patients (14.3%) were positive and among these 13 cases, 7 cases (7.7%) were from > 50-year-old patients that is classified as EBV positive DLBCL of the elderly. In previous results using ISH method, 3 of 91 patients (3.3%) were positive and 2 case (2.4%) were older than 50-year-old. These results indicate that RT-PCR method used in this study shows a higher sensitivity than ISH method. The ratio of male versus female among the EBV positive samples was 1.2:1 with the ratio of male higher. If RT-PCR method having high sensitivity is used simultaneously as well as the ISH method providing the information of the EBV positive cellular location, it is expected that EBV will be more accurately detected.

• Molecules and Cells
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• 제23권3호
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• pp.357-362
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• 2007

There is an increasing demand for high throughput (HTP) methods for gene analysis on a genome-wide scale. However, the current repertoire of HTP detection methodologies allows only a limited range of cellular phenotypes to be studied. We have constructed two HTP-optimized expression vectors generated from the red fluorescent reporter protein (RFP) gene. These vectors produce RFP-tagged target proteins in a multiple expression system using gateway cloning technology (GCT). The RFP tag was fused with the cloned genes, thereby allowing us localize the expressed proteins in mammalian cells. The effectiveness of the vectors was evaluated using an HTP-screening system. Sixty representative human C2 domains were tagged with RFP and overexpressed in HiB5 neuronal progenitor cells, and we studied in detail two C2 domains that promoted the neuronal differentiation of HiB5 cells. Our results show that the two vectors developed in this study are useful for functional gene analysis using an HTP-screening system on a genome-wide scale.

• 한국환경과학회지
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• 제28권3호
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• pp.329-339
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• 2019

The National Institute of Environmental Research (3rd KONEHs) conducted a survey of 280 residents in Ulsan, Suwon and Asan, and the concentration of heavy metals in the blood and urine were also analyzed. In case of blood lead concentration, Ulsan $2.27{\mu}g/dl$, Suwon $2.08{\mu}g/dl$, and Asan $1.75{\mu}g/dl$, the high peak in Ulsan, and the low peak in Asan. In case of cotinine concentration, Ulsan smoking($609.16{\mu}g/g{\_}ct$) is higher than nonsmoking($74.07{\mu}g/g{\_}ct$), as Suwon and Asan smoking($416.72{\mu}g/g{\_}ct$, $903.21{\mu}g/g{\_}ct$) is higher than nonsmoking($72.72{\mu}g/g{\_}ct$, $18.06{\mu}g/g{\_}ct$), smoking group is higher than nonsmoking group in all areas revealed statistically significant correlation(p<0.01). In considering results synthetically, these study results are an aid to constructing environmental health science-side heavy metal management measure education programs for normal residents.

• 대한의생명과학회지
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• 제28권3호
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• pp.186-191
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• 2022

The use of earphones has recently been widely used around the world. In currently, students wear earphones a lot in a daily life. The types of earphones are open-earphones, Canalphones, and headphones. Many students don't periodically to sterilization their earphones. Therefore, it can be an incubator that can induced ear infections. The objective of this study was to detect the pathogenic bacteria from the earphones used by the students. A total of 3 type earphones swabs were collected by sterile cotton swabs. The swabs were inoculated onto BHI agar and incubated aerobically 48 hour at 37℃. 16s rRNA PCR, electrophoresis and sequencing were performed to confirm the identification of all the bacterial isolates. As a result, 24 pathogens were identified in sequencing. Three types of earphones were sterilized in three ways: ultraviolet (UV), 70% ethyl alcohol, and antibacterial wet tissue. If you use earphones for a long time without disinfecting them for a long time, it causes various diseases such as external ear infections. The findings of this study the users periodically to sterilization their respective earphones.

• 대한의생명과학회지
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• 제29권1호
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• pp.48-52
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• 2023

Formaldehyde use is associated with serious health risks, which can affect medical personnel and technicians. Therefore, we investigated the efficacy of an alternative fixative, with respect to two types of formalin fixatives, by hematoxylin and eosin (H&E) staining, periodic acid Schiff (PAS) staining, immunohistochemical (IHC) staining, and RNA extraction. For H&E staining, the circular nucleus was stained dark blue by the basic dye hematoxylin and the cytoplasm was stained red by the acid dye eosin in all three fixative samples. No difference was found in the Duksan General Science (DGS), Sigma-Aldrich, and Core-Fix fixative samples (Corebiotech) used to fix kidney tissue, after PAS staining. IHC staining showed that CD4 was significantly increased in the lippolysaccharide (LPS)-treated group compared to the control group (vehicle), confirming the changes in specific molecules. The quantity and quality of RNA from tissues fixed in the three types of fixatives were evaluated. The average concentration of RNA was 106 ng/µL and average purity at A 260/280 ratio was 1.7~2.0, regardless of fixative used. For quality of protein, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was confirmed by Western blotting. In conclusion, Core-Fix can be used as a fixative for pathological tissues, in histological and molecular diagnoses.

• 치위생과학회지
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• 제15권1호
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• pp.12-17
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• 2015

본 연구에서는 치과 CAD/CAM 시스템을 이용하여 SM 가공 방식으로 코핑을 제작 후 주조한 금속 코핑과, AM 가공 방식으로 코핑을 제작 후 주조한 금속 코핑과의 변연 및 내면 적합도를 비교 평가하여 다음과 같은 결과를 얻었다. SM 가공 방식과 AM 가공 방식의 변연 적합도 부분에서는 $120{\mu}m$ 임상적 허용 범위에 존재하였다. SM 가공 방식과 AM 가공 방식의 변연 적합도 및 내면 적합도는 통계적으로 유의한 차이가 있는 것으로 나타났다(p<0.05). SM 가공 방식과 AM 가공 방식으로 제작된 두 그룹의 전체 적합도와 4부분 부위별 적합도 측정은 SM 가공 방식보다 AM 가공 방식이 우수하였다. 결론적으로 금속 코핑의 변연 및 내면 적합도는 SM 가공 방식보다 AM 가공 방식이 우수하였고, 그 결과 값들은 임상적 허용범위 안에 존재하였다. 앞으로 치과분야에서 AM 가공 방식에 관한 연구는 계속되어야 하며, 보다 완성도 높은 금속 코핑 제작이 가능하다고 생각된다.

• 한국환경보건학회지
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• 제40권3호
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• pp.171-177
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• 2014

Background: The development of technology can be beneficial for the life and health of human society. Crowdsourcing refers to drawing upon a large pool of individuals in order to seek services, ideas, or other contributions. With the development of information communication technology, crowdsourcing is able to provide powerful results in environmental health research. Methods: We searched 'crowdsourcing' and 'citizen science' for keywords related to the environmental health field and only selected journal articles and conference proceedings material, such as research reports and WHO reports. Results: This paper reviewed environmental health research using crowdsourcing. Examples of such research based on crowdsourcing included practices in environmental disasters, noise monitoring, global positioning system (GPS) technology, smart phones, attached portable devices and information delivery by web. Crowdsourcing methods can provide notably distinct approaches for future environmental health research. However, it is also important to protect personal information whenever crowdsourcing is applied to data generation and information dissemination. Conclusion: We expect that this review may provide useful information for the development of new environmental health research methods using crowdsourcing and citizen science.

• Journal of Periodontal and Implant Science
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• 제52권3호
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• pp.181-182
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• 2022

• 여성건강간호학회지
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• 제9권2호
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• pp.170-178
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• 2003

To identify osteoporosis and examine the relationship between health behavior and the self-efficacy of middle-aged women, a descriptive survey was conducted. The subjects were 465 healthy female residents of Kyunggido, Korea, who underwent ultrasound measurement and a health examination at the Kangmeung-Ci Health Center between July and August 2000. They were 40 to 60 years old, with a mean age of 46.7 years. The subjects completed a self-reported questionnaire that consisted of seven items concerning health behavior and twelve items concerning self-efficacy related to osteoporosis. Bone density was evaluated from ultrasound measurements of the right heel. All the data were analyzed using the program SAS-PC. The study found that the mean T score related to bone density was -1.30, and ranged from -3.52 to 3.06. Based on the T score, 74.8% of the subjects were normal, 12.9% had osteopenia, and 12.3% had osteoporosis. The mean osteoporosis self-efficacy score was 41.17 and ranged from 12 to 60. Osteoporosis self-efficacy differed significantly with health behavior related to health supplementary food (t=5.63, p=.018), exercise (t=6.65, p=.010), alcohol drinking(t=10.80, p=.001), and smoking (t=10.23, p=.001). A community-based health promotion program should be developed to prevent osteoporosis in middle-aged women.

• Perspectives in Nursing Science
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• 제3권1호
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• pp.61-72
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• 2006