• Proceedings of the Korea Society of Environmental Toocicology Conference
• /
• 2000.12a
• /
• pp.85-85
• /
• 2000

• BMB Reports
• /
• v.36 no.5
• /
• pp.468-474
• /
• 2003

The molecular mechanism of hypoxia-induced apoptosis has not been clearly elucidated. In this study, we investigated the involvement of extracellular signal-regulated protein kinase (ERK 1/2) in hypoxia-induced apoptosis using cobalt chloride in HeLa human cervical cancer cells. The cobalt chloride was used for the induction of hypoxia, and its $IC_{50}$ was $471.4\;{\mu}M$. We demonstrated the DNA fragmentation after incubation with concentrations more than $50\;{\mu}M$ cobalt chloride for 24 h, and also evidenced the morphological changes of the cells undergoing apoptosis with electron microscopy. Next, we examined the signaling pathway of cobalt chloride-induced apoptosis in HeLa cells. ERK1/2 activation occurred 6 and 9 h after treatment with $600\;{\mu}M$ cobalt chloride. Meanwhile, the pretreatment of the MEK 1 inhibitor (PD98059) completely blocked the cobalt chloride-induced ERK 1/2 activation. At the same time, the activated ERK 1/2 translocated into the nucleus and phosphorylated its transcriptional factor, c-Jun. In addition, the pretreatment of PD98059 inhibited the cobalt chloride-induced DNA fragmentation and apoptotic cell death. These results suggest that cobalt chloride is able to induce apoptotic activity in HeLa cells, and its apoptotic mechanism may be associated with signal transduction via ERK 1/2.

• Biomolecules & Therapeutics
• /
• v.25 no.5
• /
• pp.504-510
• /
• 2017

Inhibitor of nuclear factor kappa-B kinase beta ($IKK{\beta}$) plays a critical role in cell proliferation and inflammation in various cells by activating $NF-{\kappa}B$ signaling. However, the interrelationship between peroxisome proliferator-activated receptor ${\alpha}$ ($PPAR{\alpha}$) and $IKK{\beta}$ in cell proliferation is not clear. In this study, we investigated the possible role of $PPAR{\alpha}$ in the hepatic cell death in the absence of $IKK{\beta}$ gene using liver-specific Ikkb-null ($Ikkb^{F/F-AlbCre}$) mice. To examine the function of $PPAR{\alpha}$ activation in hepatic cell death, wild-type ($Ikkb^{F/F}$) and $Ikkb^{F/F-AlbCre}$ mice were treated with $PPAR{\alpha}$ agonist Wy-14,643 (0.1% w/w chow diet) for two weeks. As a result of Wy-14,643 treatment, apoptotic markers including caspase-3 cleavage, poly (ADP-ribose) polymerase (PARP) cleavage and TUNEL-positive staining were significantly decreased in the $Ikkb^{F/F-AlbCre}$ mice. Surprisingly, Wy-14,643 increased the phosphorylation of p65 and STAT3 in both Ikkb and $Ikkb^{F/F-AlbCre}$ mice. Furthermore, BrdU-positive cells were significantly increased in both groups after treatment with Wy-14,643. Our results suggested that $IKK{\beta}-derived$ hepatic apoptosis could be altered by $PPAR{\alpha}$ activation in conjunction with activation of $NF-{\kappa}B$ and STAT3 signaling.

• Journal of the Korean Institute of Gas
• /
• v.24 no.3
• /
• pp.11-19
• /
• 2020

The Occupational Health and Safety Management System (OHSMS) has been divided into OHSAS 18001 and KOSHA 18001. However as it was upgraded to ISO 45001, the international standard, on March 12, 2018, the existing OHSAS 18001 was changed to ISO 45001. In addition, the KOSHA 18001 of the Korea Occupational Safety and Health Agency was changed to KOSHA-MS, leading to conversion of the certification standard to the new certification standard. In this study, the application status of the OHSMS, introduced in Korea since 1999, was analyzed and ISO 45001 certification standard and KOSHA-MS certification standard were compared and analyzed with existing systems. Based on this, a activation plan of establishment of autonomous OHSMS when converting new standard was suggested.

• The Journal of Korean Physical Therapy
• /
• v.22 no.4
• /
• pp.73-81
• /
• 2010

Purpose: The purpose of this study was (1) to examine the effects of a Health Promotion Exercise Program (HPEP) on functional improvement, physical strength (PS) and muscle activation (MA), and the interactive influence with activities of daily living (ADL) and instrumental activities of daily living (IDAL) in the elderly, and (2) to develop and provide a HPEP for the elderly in order to prevent declines and impairments in integrated nerve function and physical capacity. Methods: Our study included relatively healthy elderly people aged 65 years or older. The experimental group, which was composed of 30 people, participated in a HPEP 50~60 minutes a day, 3 times a week, for a total of 12 weeks. Results: Confirmatory factor analysis (CFA) validated the measurement models for MA, physical strength, ADL and IADL with a p<0.01. This confirms (i) the beneficial effects of the ADFP on their MA and PS, and (ii) that their improvement in PS, in turn, can improve their IADL. The results of this study indicates that ADFP can help Korean seniors 65 years or older improve their MA, PS, ADL, and IADL, and do so synergistically. Conclusion: An HPEP, when organized in such a way that the elderly can easily do it, and when exercise items and intensity can be programmed and reorganized accordingly to individual physical and physiological characteristics by presenting 5 categorized health domains of physical strength, may be useful, especially because it can be practiced irrespective of time and place.

• Korean Journal of Applied Biomechanics
• /
• v.25 no.2
• /
• pp.219-229
• /
• 2015

Objective : The purpose of this study was to analyze EMG according to surface type and elastic band usage during the pilates Superman movement. Method : The subjects were 10 female university students with a mean age of 27. The independent variables were surface type (yoga mat, air filled equipment, elastic mat) and elastic band usage. In order to measure muscle activity, the Noraxon(USA) was used. Eight muscles (upper trapezius, lower trapezius, thoracic spine, lumbar spine, gluteus maximus, gluteus medius, biceps femoris, semitendinosus) activation were analyzed. For the statistical analysis, MANOVA, independent t-test and Scheffe test for the post-hoc via SPSS 20.0 was used. Results : The left and right upper trapezius muscle activities were significantly reduced when using the elastic bands. In addition gluteus medius muscle activities significantly increased with the elastic band as well. Conclusion : According to the usage of the elastic band and the different types of surfaces, different muscle groups were recruited. Elastic bands were found to have more activation on the gluteus medius muscle meanwhile there was less activation on the upper trapezius during the pilates Superman movement.

• Journal of Environmental Health Sciences
• /
• v.2 no.1
• /
• pp.29-31
• /
• 1975

This experiment Was carried out under two points of view, from May 1st to the end of 1973. One was the comparative determination of the activation factor of Lead dioxide by kinds in measuring of Sulfur oxides concentration by $PbO_2$ cylinder method, and the other was the comparison that result with the record of auto analyzer. Five measuring sites were selected out of Seoul City. Three kinds of Lead dioxide made in Japan (B,C and D) were compared with Standard $PbO_2$ (A for use in Determination of Sulphur in the atmosphere, purity 99% up) made in British Institution, and monthly measuring was conducted at every sampling site. The recording by auto analyzer (Beckman Model 906-A $SO_2$ Analyzer) was conducted once or twice a month for 24 hours at each sampling site during the same period. And some significant results were obtained. 1. In comparative experiments to determine the activation degree of three kinds of Lead dioxide (B,C and D), the obtained correction factor of B reagent was 1.09, 1.16 in C and 1.30 in D against Standard $PbO_2$ (A). Therefore, it should be in need of standardization or clear statement about the reagents for use, in determination sulfur oxides by $PbO_2$ cylinder method. 2. Generally, the concentration of Sulfur dioxide by wilkins' convertion method from $SO_3$ showed about 20-30% higher than those by Auto analyzer.

• Molecules and Cells
• /
• v.28 no.6
• /
• pp.589-593
• /
• 2009

In this study, the roles of the p38 MAPK, ERK1/2 and JNK signaling pathway in IGF-I-induced AR induction and activation were examined. C2C12 cells were treated with IGF-I in the absence or presence of various inhibitors of p38 MAPK (SB203580), ERK1/2 (PD98059), and JNK (SP600125). Inhibition of the MAPK pathway with SB203580, PD98059, or SP600125 significantly decreased IGF-I-induced AR phosphorylation and total AR protein expression. IGF-I-induced nuclear fraction of total AR and phosphorylated AR were significantly inhibited by SB203580, PD98059, or SP600125. Furthermore, IGF-I-induced AR mRNA and skeletal ${\alpha}-actin$ mRNA were blocked by those inhibitors in dose-dependent manner. Confocal images showed that IGF-I-induced AR nuclear translocation from cytosol was significantly blocked by SB203580, PD98059, or SP600125, suggesting that the MAPK pathway regulates IGF-I-induced AR nuclear localization in skeletal muscle cells. The present results suggest that the MAPK pathways are required for the ligand-independent activation of AR by IGF-I in C2C12 skeletal muscle cells.

• The Journal of Korean Physical Therapy
• /
• v.27 no.1
• /
• pp.1-6
• /
• 2015

Purpose: The purpose of this study was to examine the effects of a supplementary shouting technique on abdominal muscle activation to the rectus abdominis (RA) and external oblique (EO) muscles during performance of crunch exercise in healthy subjects. Methods: Sixty-four subjects were randomly allocated to two groups, crunch exercise with shouting group (SG) and crunch exercise with non-shouting group (NSG), with 32 subjects in each group, respectively. The interventions were conducted over three trials in each group, and measurements were performed on each subject by one examiner in three trials. Muscle activation of RA and EO was evaluated using electromyography (EMG) during performance of crunch exercise with or without shouting. Results: Our results showed a significantly greater increase in the EMG patterns of the RA and EO muscles during performance of crunch exercise in the SG compared to the NSG (p<0.01). Conclusion: These findings suggest that addition of the shouting technique during performance of crunch exercise, at the same time, would suggest positive evidence for improving activation of abdominal muscles.

• The Journal of Korean Physical Therapy
• /
• v.23 no.2
• /
• pp.85-90
• /
• 2011

Purpose: The contribution of the supplementary motor area (SMA) to the control of voluntary movement has been revealed. We investigated the changesin the SMA for motor learning of the reaching movement in stroke patient using functional MRI. Methods: The subject was a right-handed 55 year-old woman with left hemiparesis due to an intracerebral hemorrhage. She performed reaching movement during fMRI scanning before and after reaching training in four weeks. The motor assessment scale and surface EMG were used to evaluate the paretic upper limb function and muscle activation. Results: In the fMRI result, contralateral primary sensorimotor cortex (SM1) was activated before and after training. SMA was only activated after training. In addition, muscle activation of the paretic upper limb was similar to that of the unaffected upper limb after training. Conclusion: These findings suggest SMA is related to the execution of a novel movement pattern resulting in motor learning in stroke patients.