• Molecules and Cells
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• 제40권2호
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• pp.143-150
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• 2017

Homologous recombination (HR) is necessary for maintenance of genomic integrity and prevention of various mutations in tumor suppressor genes and proto-oncogenes. Rad51 and Rad54 are key HR factors that cope with replication stress and DNA breaks in eukaryotes. Rad51 binds to single-stranded DNA (ssDNA) to form the presynaptic filament that promotes a homology search and DNA strand exchange, and Rad54 stimulates the strand-pairing function of Rad51. Here, we studied the molecular dynamics of Rad51 and Rad54 during the cell cycle of HeLa cells. These cells constitutively express Rad51 and Rad54 throughout the entire cell cycle, and the formation of foci immediately increased in response to various types of DNA damage and replication stress, except for caffeine, which suppressed the Rad51-dependent HR pathway. Depletion of Rad51 caused severe defects in response to postreplicative stress. Accordingly, HeLa cells were arrested at the G2-M transition although a small amount of Rad51 was steadily maintained in HeLa cells. Our results suggest that cell cycle progression and proliferation of HeLa cells can be tightly controlled by the abundance of HR proteins, which are essential for the rapid response to postreplicative stress and DNA damage stress.

• 아동학회지
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• 제33권2호
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• pp.111-127
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• 2012

This study compared a number of related variables according to children's stress types using the data mining method. The sample population was taken from the Korean Youth Panel Survey (KYPS) data (2688, sixth-grade elementary students). The results of the decision tree model revealed that : (1) Parental expectations in terms of study, life satisfaction, self-esteem, parental attachment, aggression, the spousal relationship, other cognition (one's own misdeeds), and study related worries were all related to parent stress. (2) Life satisfaction, study related worries, admitting one's own misdeeds, gender, other cognition (one's own misdeeds), aggression, the spousal relationship, and a sense of alienation in the school were all related to appearance stress. (3) Study related worries, parental expectations in terms of study, aggression, life satisfaction, self-esteem, parental attachment, satisfying parental expectations, parental attachment, and teacher attachment were all related to academic stress. (4) A sense of alienation in the school, mixing with peers in the school, aggression, self-esteem, other cognition (one's own misdeeds), study related worries, parental abuse, and life satisfaction were all significantly related to friend stress. These results suggested that children's diverse conditions should be considered according to the stress types if we are to understand and cope with these stress types more efficiently.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.97-97
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• 2003

Embryonic stem (ES) cells, derived from preimplantation embryos, are able to differentiate into various types of cells consisting the whole body, or pluripotency. In addition to the plasticity, ES cells are expected to be different from terminally differentiated cells in very many ways, such as patterns of gene expressions, ability and response of the cells in confronting environmental stimulations, metabolism, and growth rate. As a model system to differentiate these two types of cells, human ES (hES, MB03) cells and terminally differentiated cells (HeLa), we examined the ability of these two types of cells in confronting a severe oxidative insult, that is $H_2 O_2$. Ratio of dying cells as determined by the relative amount of dye neutral red entrapped within the cells after the exposures. Cell death rates were not significantly different when either MB03 or HeLa were exposed up to 0.4 mM $H_2 O_2$. However, relative amount of dye entrapped within the cells sharply decreased down to 0.12% in HeLa cells when the cells were exposed to 0.8 mM $H_2 O_2$, while it was approximately 54% in MB03. Pretreatment of cells with BSO (GSH chelator) and measurement of GSH content results suggest that cellular GSH is the major defensive mechanism of hES cells. Induction of apoptosis in hES cell was confirmed by DNA laddering, induction of Bax, and chromatin condensation. In summary, hES cells 1) are extremely resistant to oxidative stress, 2) utilize GSH as a major defensive mechanism. and 3) experience apoptosis upon exposure to oxidative stress.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.78-78
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• 2003

Embryonic stem (ES) cells, derived from preimplantation embryo, are able to differentiate into various types of cells consisting the whole body, or pluripotency. In contrast, terminally differentiated cells do not usually alter their nature but frequently die or transform if they are exposed to inappropriate external stimulations. In addition to the plasticity, ES cells are expected to be different from terminally differentiated cells in very many ways, such as patterns of gene expressions, ability and response of the cells in confronting environmental stimulations, metabolism, and growth rate. As a model system to differentiate these two types of cells, human ES cells (MB03) and terminally differentiated cells (HeLa), we examined the ability of these two types of cells in confronting a severe oxidative insult, that is $H_2O$$_2$. Approximately 1$\times$10$^4$ cells were plated in 96 well plate and serum starved for overnight. The conditioned cells were exposed to a various concentration of $H_2O$$_2$ fur 24 hrs and loaded with neutral red (50$\mu\textrm{g}$/ml) for 4 hrs, washed with PBS for 2 min three times, and entrapped dye was dissolved out using acetic ethanol. Cytotoxicity was determined by reading the amount of dye in the medium using microplate reader. equipped with 575 nm filter. Relative amount of the dye entrapped within MB03 or HeLa were not significantly different when cells were exposed up to 0.4 mM $H_2O$$_2$. However, this sharply decreased down to 0.12% in HeLa cells when the cells were exposed to 0.8 mM $H_2O$$_2$, while it was approximately 54% in MB03 suggesting that this concentration of $H_2O$$_2$ is the defensive threshold for HeLa cells. The resistance to oxidative stimulation reversed, however, when cells were co-treated with BSO (L-buthionine- 〔S, R〕-sulfoximine) which chelates intracellular GSH. This result suggests that cellular GSH is the major defensive mechanism of human ES cells. Induction of enzymes involved in GSH metabolism and type of cell death is currently being studied.

• 한국학교보건학회지
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• 제11권1호
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• pp.63-74
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• 1998

The purposes of this study are to show in interpersonal relations what factors male students are stressed from, how they experience stress, how they cope With it in each situation, and what this results in It attempt to gam basic materials to promote male student health and positive coping methods, The subject of this study was 15 male students of C University located in Seoul and Kyeonggui-Do They were composed of 5 sophomores, 5 Juniors and 5 seniors The period for collecting materials was October 1997 to January 1998, and the interview time ranged from 50 minutes to two hours The interview frequency was one to three times as occasion demanded, The materials were analyzed by the methods and theory suggested by Strauss & Corbin (1990) The results were 130 categories grouped into 33 divisions by similarity Finally, they were united into 9 higher categories In interpersonal relations the core category of male student stress is "affliction", and it follows the course of generation-coping-resolution The types showed in the course of material analysis are as follows, (1) When the subject student is on good terms with the other and the stress is repeated- "affliction" is strong and continuous-and his ability sense is strong, he copes with "affliction" With his own will and solves It affirmatively, (2) When the subject student is on good terms with the other and the stress is repeated-so "affliction" is strong and continuous-and his ability sense is weak, he copes with "affliction" with an emotional bias and solves it negatively (3) When the subject student is on good terms with the other and the stress is temporary-"affliction" is strong and temporary-and his ability sense is strong, he copes with "affliction" with his own will and solves it affirmatively (4) When the subject student becomes estranged from the other and the stress is temporary-"affliction" is weak and temporary-and his ability sense is weak, he copes with "affliction" with an emotional bias and solves it negatively, (5) When the subject student becomes estranged from the other and the stress is repeated-"affliction" is strong-and continuous and his ability sense is strong, he copes with "affliction" with his own will and solves it affirmatively (6) When the subject student becomes estranged from the other and the stress is repeated-"affliction" is strong and continuous-and his ability sense is weak, he copes with "affliction" with an emotional bias and solves it negatively. According to the above results, the conditions of cause and effect for male students to generate "affliction" should be understood in order to help cope with stress caused by interpersonal relations A program for education and counseling should be developed for male students to strengthen their 'ability sense' in choosing coping strategies In addition, the individual estimation for ability sense should be performed when education and counseling them.

• 한국지역사회생활과학회지
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• 제19권4호
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• pp.547-558
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• 2008

The purpose of this study was to identify a couple's parental efficacy combination and, then, analyze parental role-sharing and parenting stress according to this combination type. The subjects were 307 couples who have a child in 1st or 2nd grade. The main results were as follows: First, fathers and mothers, as a group, did not differ in terms of parental efficacy. Secondly, some demographic variables related to parental efficacy. Thirdly, there are more couples who have similar levels of parental efficacy than those who have different levels of parental efficacy. Fourth, no significant difference in parental role-sharing was found among the 4 types of couple's parental efficacy. Fifth, mothers had low parental stress when both of the couple had high levels of parental efficacy while fathers perceived low parental stress if only he, himself, had a high level of parental efficacy.

• 구강회복응용과학지
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• 제19권3호
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• pp.219-237
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• 2003

he purpose of this study was to compare the fracture resistance of the IPS Empress ceramic crown with 1.0mm width rounded shoulder, which is usually recommended in all ceramic crown, and 0.5mm width chamfer finish lines on the maxillary first premolar. 30 sound maxillary first premolars were selected and then storaged in 5% NaOCl and saline. 15 teeth were performed preparation for each group(1.0mm rounded shoulder, 0.5mm chamfer). After 30 stone dies were made for each group, the IPS Empress ceramic crowns were fabricated and cemented with resin cement(Bistite resin cement, Tokuyama Soda Co. LTD., Japan) on the natural teeth. The cemented crowns were mounted on the positioning jig and the universal testing machine(Zwick Z020, Zwick Co., Germany)was used to measure the fracture strength, with stress loading on the occlusal surface between buccal and lingual cusp. And also, three-dimensional finite element model was used to measure the stress distribution with two types of the finish lines(1.0mm rounded shoulder, 0.5mm chamfer) and two loading conditions(both buccal and lingual cusp inclination, lingual cusp inclination only). The result of the this study were as follows. In the fracture resistance experiment according to the finish line, the mean fracture strength of rounded shoulder(842N) showed higher value than that of the chamfer(590N) (p<0.05). In the three dimensional finite element analysis of all ceramic crown, metal die and natural teeth model did not show any differences in stress distribution between finish lines. Generally, when force was loaded on the occlusal inclination of buccal and lingual cusp, the stress was concentrated on the loading point and the central groove of occlusal surface. When force was loaded only on the occlusal inclination of lingual cusp, the stress was concentrated on the lingual finish line and loading point.

• 대한치과보철학회지
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• 제31권4호
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• pp.611-624
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• 1993

The purpose of this study was to analyze the stress distribution of the natural teeth, the implant, the prosthesis and the supporting tissue according to the types of implant and connection modality in the five-unit fixed partial denture with a implant pier abutment. A Two dimensional stress analysis model was constructed to represent a mandible missing the first and second premolars and first molar. The model contained a canine and second molar as abutment teeth and implant pier abutments with and without stress-absorbing element. Finite element models were created and analyzed using software ANSYS 4.4A for IBM 32bit personal computer. The results obtained were as follows. 1. Implant group, compared to the natural teeth group, showed a maximum principal stress at the superior portion of implants and a stress concentration at :he neck and end portion. 2. Maximum principal stress and maximum Von Mises stress were always lower in the case of rigid connection than nonrigid connection. 3. A cylinder type implant with stress absorbing element and screw type implant were generally similar in the stress distribution pattern. 4. A screw type implant, compared to the cylinder type implant, showed a relatively higher stress concentration at both neck and end portion of it. 5. Load B cases showed higher stress concentration on the posterior abutments in the case of nonrigid connector than rigid connector. 6. A maximum displacement was always lower in the case of rigid connection than nonrigid connection. These results suggest that osseointegrated implant can be used as an intermediate abutment.

• 대한치과교정학회지
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• 제34권2호
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• pp.121-129
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• 2004

이 연구의 목적은 세 가지 형태의 헤드기어를 이용하여 상악 제1대구치의 후방이동 시 치아 주위 조직에 나타나는 응력분포를 광탄성법을 이용하여 관찰하는 것이다. 광탄성 상악모형에 장착한 헤드기어에 high-pull, straight-pull, cervical-pull의 세 가지 방향에서, face bow의 inner bow와 outer bow를 평행하게 한 상태에서 outer bow의 길이를 세 가지로 다르게 하여 적용시킨 다음 원편광기를 이용하여 응력분포를 분석하였다. 사진기를 이용하여 응력분포를 기록하고, 등색선상의 무늬차수를 이용, 응력분포를 분석하여 이차원적인 광탄성 응력분석을 시행하였다. 그 결과는 다음과 같다. 1. high-pull 헤드기어의 경우, 중간 길이의 outer bow일 때 치체이동에 유리한 응력 분포가 나타났다 2. straight-pull 헤드기어의 경우, 긴 길이의 outer bow일 때 좀더 치체이동에 유리한 응력 분포가 나타났으나, outer bow의 길이에 따른 응력분포의 변화는 적었다. 3. cervical-pull 헤드기어의 경우, 긴 길이의 outer bow일 때 치체이동에 유리한 응력 분포가 나타났다. 임상에서 헤드기어를 이용하여 상악 제1대구치를 후방으로 치체이동시키기 위해서는 facebow outer bow의 높이를 조정하지 않는 경우에는 high-pull 헤드기어에서는 outer bow와 inner bow를 같은 길이로, straight-pull 및 cervical-pull 헤드기어에서는 outer bow를 더 길게 하여 사용하는 것이 추천된다.

• 대한간호학회지
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• 제20권2호
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• pp.214-226
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• 1990

For a man to maintain attention, he needs to keep a certain level of arousal. An inordinate increase or decrease in the level of arousal eventually has a negative influence on attention. Precedent research has shown that the degree of attention changes when an experience of stress is related to anxiety resulting in a rise in arousal. This research was done to examine this hypothesis by looking at the 27 female students, 14 of whom had failed in the annual examination. The results of the investigation are as follows : The stress of failure in the examination was seen to raise the level of physiological arousal. Although pulse and electromyography showed no significant change, further inquiries should be made based on other types of methodology. In spite of the rise of arousal, the performance of selective task was degraded. This suggests those students failed to give moderate attention to given information for that kind of task. But the exact reason of that failure was not identified : that is it was difficult decide whether they gave too much attention to the anxiety brought about by stress. Performance of integral tasks, however, did not show any degradation. Judging from these results, stress seems to exert significant influence on attention in the selection of the appropriate information among the various options given. This offers an important hint in relation to the health care situation where nursing information is offered. Clients who receive nursing information in stressful situations may have difficulty in separating and selecting this helpful information from other options which they have acquired through their life experience. The content and terminology of nursing information may be strange and unintelligible to clients, although they are quite familiar and distinct to nurses. So, it is desirable for nurses to give, in addition and at the same time when nursing information is given, some certain related information as devices for selection, instead of merely giving nursing informations as such. So far it is not clear whether the concepts of information processing theory can be suitably applied to nursing. However, it is obvious, according to this research, that the quality of attention is disturbed in the stress situation. This is why further inquiries should be made into attention in practical nursing situation.