• Journal of Microbiology
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• 제34권2호
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• pp.179-183
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• 1996

Streptoccus Pneumoniae (pneumococcus), the most common cause of bacterial pneumonia, has an ample polysaccharide (PS) capsule that is highly antigenic and is the source of PS vaccine. This investigation was undertaken to optimize the culture conditions for the production of capsulard PS by type 1 pneumococcus. Among several culture media, brain heart infusion (BHI) and Casitone based media were found to support luxuriant growth of pneumococcus type 1 at the same level. Because BHI medium is rather expensive and more complex than the Casitone based media, the Casitone based media was uwed to study optimization of the culture condition. The phase of growth which accomodated maximum PS production was logarithmic phase. Concentrations of glucose greater than 0.2% did not ehnahce growth or PS production. Substitution of netrogen sources with other resources or supplementation of various concentrations of metal ion (with the exception of calcium ion) had adverse affects on growth and PS production. On the other hand, low level aeration was beneficial for increased PS production. Addition of 3 mg/1 concentration of methionine, phenylalanine, and threonine were found to enhance growth and PS production. The synerigistic effect of all the favorable conditions observed in pneumococcal growth assays provided a two-fold cummulative increase in capsular PS production.

• 대한의용생체공학회:의공학회지
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• 제32권3호
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• pp.185-190
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• 2011

The purpose of this study is to investigate the influence of organism vigor information solution(OVIS) on the animal cell growth and to set up a condition for cell culture. We investigated the reactions on the MG-63 and MCF-7 cell line in mixture culture media with various amount of REVIEW solution, which is an example among various OVIS. DMEM-HG was used as basic media. The concentration range of the mixture was limited from 0% to 15%. MTT assay is used for cell viability test. The cell was incubated for 14days and the MTT assay was performed on day 1, 3, 7, 10 and 14 throughout the experiment. We used the ELISA reader to measure the Optical Density(O.D) at 595 nm wavelength filter. MCF-7 was linearly proliferated according to culture time and concentrations of OVIS. On the other hand, MG-63 cells were measured the highest O.D value at 12%. The growth rates of both cells cultured in mixed culture media with OVIS are much higher than those in only basic media, DMEMHG, after 14days. It was confirmed that cell cultured at OVIS grows rapidly at certain period although cells showed a negative effect in initial stage.

• Journal of Microbiology and Biotechnology
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• 제22권3호
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• pp.400-406
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• 2012

To improve the hydrogen yield from biological fermentation of organic wastewater, a co-culture system of dark- and photo-fermentation bacteria was investigated. In a pure-culture system of the dark-fermentation bacterium Clostridium butyricum, a pH of 6.25 was found to be optimal, resulting in a hydrogen production rate of 18.7 ml-$H_2/l/h$. On the other hand, the photosynthetic bacterium Rhodobacter sphaeroides could produce the most hydrogen at 1.81mol-$H_2/mol$-glucose at pH 7.0. The maximum specific growth rate of R. sphaeroides was determined to be 2.93 $h^{-1}$ when acetic acid was used as the carbon source, a result that was significantly higher than that obtained using either glucose or a mixture of volatile fatty acids (VFAs). Acetic acid best supported R. sphaeroides cell growth but not hydrogen production. In the co-culture system with glucose, hydrogen could be steadily produced without any lag phase. There were distinguishable inflection points in a plot of accumulated hydrogen over time, resulting from the dynamic production or consumption of VFAs by the interaction between the dark- and photo-fermentation bacteria. Lastly, the hydrogen production rate of a repeated fed-batch run was 15.9 ml-$H_2/l/h$, which was achievable in a sustainable manner.

• 패션비즈니스
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• 제17권4호
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• pp.22-39
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• 2013

This thesis aims to investigate the characteristics of the civil costume in Netherlands women and the way how the civil culture was reflected on these by analyzing the women's costume depicted in the works of the Johannes Vermeer regarded as the representative painter of Netherlands in 17th century. The method of study was to select 24 pieces of Vermeer's works among the 30 pieces, and were analyzed in detail. These were approved to be common civil costumes by researching the works of other painters in that era on the other hand. The result shows that the civil costume of Netherlands women from 1653 until 1675 was in the simple form of two pieces dress, and minimal decorations with simple hair style and headdress would take the constitution of the frugality, chastity and practicality as the mainstream, along with using the red, yellow and blue as the primary three colors on to the costumes. These characteristics might be ascribed to the Netherlands civil culture influenced by the Calvinism that emphasized the frugality and chastity, denouncing the luxury with supporting the religion and morality. This trend was also noted in the men's costume, giving evidence of the intimate relationship between the costume, religion and civil culture. This thesis might be a help to elucidate the relationship between the costume and cultural society, and be a affordable tools to study the contemporary costume.

• 한국산학기술학회논문지
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• 제17권1호
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• pp.252-263
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• 2016

본 논문에서 유교문화콘텐츠를 기반으로 도시 브랜드화 전략을 통해 도시가 활성화된 사례를 분석하고 도시 브랜드의 가치 향상과 도시의 활성화 요인을 고찰하고자 한다. 유수의 도시들이 문화자원을 통해 경쟁력을 확보하고 있으며 특히 전통문화는 차별성을 확보해주는 중요한 자산으로 그 가치가 더욱 부각되고 있다. 우리나라 문화 전반에 흐르고 있는 유교는 건축학적인 문화유산 뿐 만 아니라 예법, 문묘제례, 교육, 음식 등의 다양한 콘텐츠를 담고 있어 활용가치가 높은 문화자원이다. 그러나 대다수의 유교문화자원은 보존 및 운영에 어려움을 겪고 있으며, 무형의 자산 또한 활성화되지 못하고 방치되고 있는 실정에 있어 이를 지역의 특색에 맞게 특성화하여 도시의 브랜드화 전략에 적극적인 활용이 필요하다. 이를 위해 대표적인 유교문화도시인 안동시와 중국 산동 곡부시의 사례를 살펴봄으로서 유교문화콘텐츠를 활용한 도시브랜드 활성화 방안을 도출하고자 한다.

• Parasites, Hosts and Diseases
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• 제49권3호
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• pp.295-298
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• 2011

Lactobacillus species in the female genital tract are thought to act as a barrier to infection. Several studies have demonstrated that lactobacilli can adhere to vaginal epithelial cells. However, little is known about how the adherence of lactobacilli to vaginal epithelial cells affects the acidity, cell viability, or proliferation of the lactobacilli themselves or those of vaginal epithelial cells. Lactobacillus acidophilus was co-cultured with immortalized human vaginal epithelial cells (MS74 cell line), and the growth of L. acidophilus and the acidity of the culture medium were measured. MS74 cell density and viability were also assessed by counting cell numbers and observing the cell attachment state. L. acidophilus showed exponential growth for the first 6 hr until 9 hr, and the pH was maintained close to 4.0-5.0 at 24 hr after culture, consistent with previous studies. The growth curve of L. acidophilus or the pH values were relatively unaffected by co-culture with MS74 cells, confirming that L. acidophilus maintains a low pH in the presence of MS74 cells. This co-culture model could therefore potentially be used to mimic vaginal conditions for future in vitro studies. On the other hand, MS74 cells co-cultured with L. acidophilus more firmly attached to the culture plate, and a higher number of cells were present compared to cells cultured in the absence of L. acidophilus. These results indicate that L. acidophilus increases MS74 cell proliferation and viability, suggesting that lactobacilli may contribute to the healthy environment for vaginal epithelial cells.

• 정보관리학회지
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• 제40권4호
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• pp.329-349
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• 2023

정보통신 기술이 빠르게 발전하면서 데이터의 생산 속도가 급증하였고, 이는 빅데이터라는 개념으로 대표되고 있다. 단시간에 데이터 규모가 급격하게 증가한 빅데이터에 대해 품질과 신뢰성에 대한 논의도 진행되고 있다. 반면 스몰데이터는 품질이 우수한 최소한의 데이터로, 특정 문제 상황에 필요한 데이터를 의미한다. 문화예술 분야는 다양한 유형과 주제의 데이터가 존재하며 빅데이터 기술을 활용한 연구가 진행되고 있다. 하지만 문화예술기관의 기본정보가 정확하게 제공되고 활용되는지를 탐색한 연구는 부족하다. 기관의 기본정보는 대부분의 빅데이터 분석에서 사용하는 필수적인 근거일 수 있고, 기관을 식별하기 위한 출발점이 된다. 본 연구는 문화예술 기관의 기본정보를 다루는 데이터를 수집하여 공통 메타데이터를 정의하고, 공통 메타데이터를 중심으로 기관을 연계하는 지식그래프 형태로 스몰데이터를 구축하였다. 이는 통합적으로 문화예술기관의 유형과 특징을 탐색할 수 있는 방안이 될 수 있다.

• 한국의료질향상학회지
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• 제21권1호
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• pp.36-49
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• 2015

In Intensive Care Units (ICUs), where severely ill patients are treated, importance of reducing Hospital Acquired Infection (HAI) cannot be overstated. One of the simplest and most effective actions against HAI is proper hand hygiene (HH) behavior of Health Care Workers (HCWs). However, compliance varies across different cultures and different job types of HCWs (physicians, residents and nurses). This study aims to understand determinants of HH behavior by HCWs' job types in Korea. Qualitative analysis was performed based on Reasoned Action Approach style interviews with staff physicians, residents and nurses across 7 teaching hospitals. We found that all HCWs strongly believe HH is important in reducing HAI. There were, however, job type-specific HH behavior modifying factors; staff physicians stated feeling pressure to be HH behavior role model. Residents identified Quality Improvement team that measured compliance as a facilitator; a notable barrier for residents was senior physicians not washing their hands, because they were afraid of appearing impudent to their seniors. Nurses designated their chief nurse as a key referent. All participants mentioned heavy workload and lack of access to alcohol-based sanitizer as situational barriers, and sore and dry hand as deterrents to HH compliance.

• 한국의류학회지
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• 제31권1호
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• pp.57-67
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• 2007

The purpose of this study is to find out the better washing condition, especially on detergent contents to satisfy the consumer's concern on fabric hand using Drum Type Washer. The hand and preference of washed fabrics by various detergent contents were analyzed through subjective evaluation using questionnaire method in dry and wet state. Wine rank's semantic differential scale questions were developed with 27 kinds of adjective pairs and seven rank's scale questions were to evaluate preference of washed fabrics oil holistic touch, washing and rinsing effect and purchase intention of detergent. Group of trained panelists and untrained women panelists of $30{\sim}40$ years old were participated. The factors affecting consumer's taste for the washed fabrics were analyzed by SPSS 12.0. Smoothness showed relatively higher correlation with preference of washed fabrics on holistic touch, washing and rinsing effect and purchase intention of detergent. There were significant differences in preference of washed fabrics on holistic touch, washing and rinsing effect and purchase intention of detergent by detergent contents when tested in wet state. Fabrics washed with detergents of non-zeolite were appeared to be the preferred ones.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2009년도 제38회 동계학술대회 초록집
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• pp.44-45
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• 2010

We are currently conducting studies on culturing and biocompatibility assessment of various cells such as neural stem cells and induced pluripotent stem cells(IPS cells) on carbon nanotube (CNT), on nerve regeneration electrodes, and on silicon wafers with a focus on developing nerve integrated CNT based bio devices for interfacing with living organisms, in order to develop brain-machine interfaces (BMI). In addition, we are carried out the chemical modification of carbon nanotube (mainly SWCNTs)-based bio-nanosensors by the plasma ion irradiation (plasma activation) method, and provide a characteristic evaluation of a bio-nanosensor using bovine serum albumin (BSA)/anti-BSA binding and oligonucleotide hybridization. On the other hand, the researches in the case of "novel plasma" have been widely conducted in the fields of chemistry, solid physics, and nanomaterial science. From the above-mentioned background, we are conducting basic experiments on direct irradiation of body tissues and cells using a micro-spot atmospheric pressure plasma source. The device is a coaxial structure having a tungsten wire installed inside a glass capillary, and a grounded ring electrode wrapped on the outside. The conditions of plasma generation are as follows: applied voltage: 5-9 kV, frequency: 1-3 kHz, helium (He) gas flow: 1-1.5 L/min, and plasma irradiation time: 1-300 sec. The experiment was conducted by preparing a culture medium containing mouse fibroblasts (NIH3T3) on a culture dish. A culture dish irradiated with plasma was introduced into a $CO_2$-incubator. The small animals used in the experiment involving plasma irradiation into living tissue were rat, rabbit, and pick and are deeply anesthetized with the gas anesthesia. According to the dependency of cell numbers against the plasma irradiation time, when only He gas was flowed, the growth of cells was inhibited as the floatation of cells caused by gas agitation inside the culture was promoted. On the other hand, there was no floatation of cells and healthy growth was observed when plasma was irradiated. Furthermore, in an experiment testing the effects of plasma irradiation on rats that were artificially given burn wounds, no evidence of electric shock injuries was found in the irradiated areas. In fact, the observed evidence of healing and improvements of the burn wounds suggested the presence of healing effects due to the growth factors in the tissues. Therefore, it appears that the interaction due to ion/radicalcollisions causes a substantial effect on the proliferation of growth factors such as epidermal growth factor (EGF), nerve growth factor (NGF), and transforming growth factor (TGF) that are present in the cells.