• Title/Summary/Keyword: hand culture

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Production of Pigments and $\alpha$-Tocopherol by Cell Cultures in Safflower (Carthamus tinctorius L.)

  • Gao, Wen-Yuan;Seon, Jeong-Hoon;Son, Sung-Ho;Maurice Moloney;Paek, Kee-Yeoup
    • Journal of Plant Biotechnology
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    • v.1 no.2
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    • pp.69-77
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    • 1999
  • Safflower is an important medicinal plant that has been used in China, Korea and Japan for thousands of years. The red and yellow pigments obtained from the petals of safflower can invigorate blood, release stagnation and promote menstruation. In addition, these pigments are used safely in processed foods and soft drinks as naturally harmless rotor additives. On the other hand, the seed of safflower contains 30-40% oil with higher level of mono- and poly-unsaturated fatfy acid profiles and elevated levels of $\alpha$-tocopherol. In this paper, we describe advances in the production of pigments and $\alpha$-tocopherol by cell culture in safflower.

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The Linkage between the U.S. Commercial Media System and the Crisis of the Korean Media Industry

  • Shim, Doo-Bo
    • International Journal of Contents
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    • v.6 no.2
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    • pp.28-32
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    • 2010
  • Reports on the Korean culture's gaining popularity among foreign audiences have filled the Korean news media for more than a decade by now. On the other hand, some observers have begun to argue that the hyper-commercialism has brought the Korean cultural industries to a crisis. By examining a brief history of U.S. commercial media system's global spread, this paper makes an effort to understand the Korean media industry within an international and historical framework. Secondly, it analyzes the processes of Korean media industry losing its competitiveness following the Korean Wave success. Based on the research findings, this paper warns against the potentially detrimental effects of commercial media system.

The Content Analysis of the Korean Food Menu Naming Standard (한식 메뉴 명명 기준에 대한 내용분석)

  • Han, Kyung-Soo;Lee, Jin-Young
    • Journal of the Korean Society of Food Culture
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    • v.26 no.6
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    • pp.629-640
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    • 2011
  • This research analyzed the naming standard of Korea menu names divided into two groups, main dish and side dish. The research was conducted by contents analysis of selected literature articles and multiple-response cross tabulation analysis. The result demonstrated that the naming standard of Korea food consisted of the main ingredient name - sub ingredient name - main condiment name and main recipe. On the other hand, the menu name that is in native language or has a historical origin is exempt from this classification. Therefore, this study proposes a new standard, 'Hansik Menu Naming', to assist the food service industry and correct the names of unknown foreign dishes.

RNN NARX Model Based Demand Management for Smart Grid

  • Lee, Sang-Hyun;Park, Dae-Won;Moon, Kyung-Il
    • International Journal of Advanced Culture Technology
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    • v.2 no.2
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    • pp.11-14
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    • 2014
  • In the smart grid, it will be possible to communicate with the consumers for the purposes of monitoring and controlling their power consumption without disturbing their business or comfort. This will bring easier administration capabilities for the utilities. On the other hand, consumers will require more advanced home automation tools which can be implemented by using advanced sensor technologies. For instance, consumers may need to adapt their consumption according to the dynamically varying electricity prices which necessitates home automation tools. This paper tries to combine neural network and nonlinear autoregressive with exogenous variable (NARX) class for next week electric load forecasting. The suitability of the proposed approach is illustrated through an application to electric load consumption data. The suggested system provides a useful and suitable tool especially for the load forecasting.

Studies on Cellulase Induction in Myriococcum albomyces (Myriococcum albomyces에 있어서 Cellulase 유도생성에 관한 연구)

  • Chung, Dong-Hyo
    • Korean Journal of Food Science and Technology
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    • v.3 no.1
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    • pp.1-5
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    • 1971
  • 1. Formation of cellulase in Myriococcum albomyces was investigated using shaking culture with addition of CMC or Avicel as an inducer to 5% wheat bran medium. 2. Three different types of cellulase fraction I, fraction II and fraction III in the culture filtrate were purified by elution column chromatography on a DEAE-Sephadex A-25. 3. By the addition of CMC as an inducer, CMCase activity was stronger than that of Avicelase. On the other hand, the addition of Avicel increased Avicelase activity.

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Effects of Edible Herbs on the Growth of In Vitro Intestinal Microorganisms (산채류가 장내세균의 In Vitro 생육에 미치는 영향)

  • 한복진
    • Journal of Nutrition and Health
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    • v.27 no.7
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    • pp.717-728
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    • 1994
  • This study was aimed to screen edible herbs which control the composition of intestinal microflora. With in vitro experiments, we screened the water or ethanol extracts of about 60 edible herbs and wild plants in terms of the inhibition activity on the growth of the harmful Clostridium perfringens and growth promoting activity for the beneficial Bifidobacteria. The water extracts of mugwort and small water dropwort inhibited the growth of Cl.perfringens both in agar diffusion method and broth culture. On the other hand, the water extracts of petasites, mugwort, yellow day-lily and bitter cress have shown the promotion effect on the growth of Bifidobacterium longum. In the culture test using human feces as starter, the extracts of the above selected herbs increased the population of Bifidobacteria and Lactobacillus while they reduced the numbers of Cl.perfringens and E.coli.

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Ginseng Tissue Culture (인삼(人蔘)의 조직배양(組織培養))

  • Lee, Jae-Du
    • Korean Journal of Pharmacognosy
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    • v.3 no.2
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    • pp.65-72
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    • 1972
  • Tissues of $Panax\;Schinseng\;N_{EES}\;root$ were cultured on the synthetic agar media to investigate the nutrient efficiency on the callus induction and organ formation. The differentiation pattern of the callus mass and the structure of the induced organ (root) were observed internally. On White's medium, callus formation needed the supplement of 2,4-D (5mg/l) and kinetin (1.0mg/l), and on MS medium the root induction NAA (0.2mg/l) and kinetin (0.1mg/l). In order to investigate the effect of inorganic components on callus formation, the inorganic part of White' medium was substituted with those of Heller, Murashige Skoog, and Earle. As the result culture Earle's was most effective. On the other hand, the roots were induced from the meristem in the deep region of callus mass. Since this meristem is similar to the pericambium of tap root, they are the same on the pattern of morphogenesis.

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A Study on the Splitting Methods in Mouse and Bovine Embryos (생쥐 및 소 수정란의 분할방법에 관한 연구)

  • 조남기
    • Korean Journal of Animal Reproduction
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    • v.11 no.2
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    • pp.127-131
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    • 1987
  • This study was carried out to obtain the basic information on splitting and culture of mouse and bovine embryos. Two-, four-, eight-, cell and morula mouse embryos were digested with pronase, splitted in vitro by micro-glass needel with hand, and bovine embryos were splitted by micromanipulator. The splitted embryos were cultured under 5% of CO2 gas in air at 37$^{\circ}C$ for 48-72 hours. The results obtained in this study were summarized as follows: 1. The mouse and cattle were superovulated by 5IU of PMS and HCG, and 2500IU of PMS and 25mg of PGF2$\alpha$, respectively. The average number of embryos after superovulation were 32.5$\pm$8.2 and 7.5$\pm$3:1, respectively. 2. Out of total 122 embryos splitted, the successful splitting rate was 75.0%, 66.7%, 68.4% and 71.4% in 2-, 4-, 8- and morula embryos in mouse, respectively. There was no different splitting rate between mouse(71.4%) and bovine embryos(66.7%) in morula. 3. The successful culture rate of splitted embryos was 68.0% and 67.9% in mouse and bovine embryos, respectively.

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On a highly proteolytic mutant strain of Aspergillus flavus (Aspergillus flavus의 강력 protease생성 돌연변이의 유발)

  • 이영녹;박용근;고상균
    • Korean Journal of Microbiology
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    • v.18 no.2
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    • pp.51-58
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    • 1980
  • Mutational experiments were performed to improved to improve the protease productivity of Aspergillus flavus KU 153, which is selected among the wild strains. A UV-induced mutant strain having high protease productivity was obtained by the use of the clear zone method as a simple criterion for a primary screening test. Neutral and alkaline protease activities of hte mutant strain were higher than 1.8 times, comopared with those of the parental strain, respectively, while in the case of acid protease, it was 2.7 times. The mutant strain selected was more powerful in the production of cellulase and amylase, as well s protease in wheat bran, compared with those of the parental strain. protease production of the parental strain has reached maximum level at 3 days culture, while alkaline nad neutral protease production of the mutantstrain has reached at 2 days culture. On the other hand, the mutant strain formed the spore slowly, compared with the parental strain. Column chromatography of the neutral protease on DEAE-Sephadex A-50 showed that the mutant strain was not induced the formation of another neutral protease isozyme, but induced the variation in the function of regulatory gene.

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Managerial Factors Affecting the Community Spirit and Participation of Medical and Psychiatric Social Workers (보건의료영역 사회복지사의 전문직 공동체의식과 참여의식에 대해 조직 관리적 요인이 미치는 영향)

  • Kim, Dong Won;Cho, Nam-Shin
    • Korea Journal of Hospital Management
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    • v.18 no.2
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    • pp.104-126
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    • 2013
  • The goal of the study is to find what managerial characteristics affect the community spirit and participation of medical and psychiatric social workers. To do this, managerial characteristics were divided into organizational structure and culture as well as job satisfaction. As a result of an empirical study, while Herzberg's motivators or mental rewards, such as autonomy, sense of accomplishment, and sense of respect, improved the levels of community spirit and participation, Herzberg's hygiene factors or economic rewards did not have any relationship with them statistically. This means that mental rewards equivalent to those of other professions in hospitals will improve the professional identity and activities of social workers, who are working with diverse professions in hospitals. On the other hand, organizational structure and culture were not significant statistically.

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