• 한국미생물·생명공학회지
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• 제27권3호
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• pp.203-207
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• 1999

V. alginolyticus 138-2, a marine halophilic bacterium, produced an extracellular amylase with a molecular weight of ca. 56,000. The analysis of the digestion products of soluble starch by thin layer chromatography(TLC) revealed that the extracellular amylase of V. alginolyticus 138-2 is a saccharifying-type alpha-amylase. The alpha-amylase activity of the culture supernatant of soluble starch was optimal at pH 6.0 and 45$^{\circ}C$. Ca2+ slightly increased the alpha-amylase activity, whereas Hg2+, An2+, Cu2+, Ni2+, Fe2+, and Mn2+inhibited the enzymatic activity. Alkylating thiol group agent, iodoacetic acid did not affect the alpha-amylase activity, but reduced thiol reagents such as dithiothreitol, cysteine, and beta-mercaptoethanol stimulated theenzymatic activity. On the other hand, even if V. alginolyticus 138-2 is a marine halophilic bacterium, its alpha-amylase activity was significantly inhibited by NaCl.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.710-712
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• 2003

A new species of Gram-negative halophilic cartenoid producing bacterium was isolated from the Haeundae Coast, Korea. This strain is non-motile, aerobic, orange-pigmented, rod-shaped, and produced carotenoids, mainly astaxanthin. All the type strains of the genus Paracoccus were compared with this strain using 16S rDNA sequence analysis, fatty acid patterns, and physiological reaction profiles. From the results obtained, this strain is classified as a new species, Paracoccus sp..

• 미생물학회지
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• 제41권2호
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• pp.112-116
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• 2005

호염성세균인 Kordia algicida gen. nov,, sp. nov.은 규조류인 Skeletonema costatum에 의한 적조발생동안 마산만의 표층수로부터 분리되었다. 본 균주는 숙성해수가 공급된 ZoBell 2216e 배지에서 성장이 가능하나 $3\%$ NaCl만이 공급된 동 배지에서는 성장하지 않았다. 13종의 주된 재수성분을 대상으로 한 염류 요구성 조사에서 $Na^+,\;Mg^{2+}$ 및 $Ca^{2+}$ 이온이 제한된 배지에서는 성장을 하지 않아, 성장을 위해 필수적으로 3조의 이온을 요구하였다. 3종의 이온을 대상으로 한 Kinetic 조사에서 $Na^+,\;Ca^{2+}$ 및 $Mg^{2+}$ 의 Km 간은 각각 0.202 M, 0.089 mM, and 0.189 mM로 결정되었으며 $V_{max}({\mu}max)$는 또한 0.442h, 0.411h 및 0.316h로 결정되었다. 현미경적 관찰에서 K. algicida는 각 이온들을 제한하였을 때 $2\~8$시간에 내에 빠른 사멸을 나타내었다. 이러한 결과로 Kordia algicida는 해양으로부터 유래된 전형적인 호염성세균임을 알 수 있었으며 해양유래 미생물의 분리 시 각별한 주의가 요구됨을 시사한다.

• Journal of Microbiology and Biotechnology
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• 제16권12호
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• pp.1862-1867
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• 2006

A moderately halophilic, Gram-positive, spore-forming bacterium was isolated from the roots of Blutaparon portulacoides, a plant found in sandy soil parallel to the beach line in Restinga de Jurubatiba, Rio de Janeiro, Brazil. The strain, designated $M9^T$, was motile and strictly aerobic with rod-shaped cells. It grew in the absence of NaCl and up to 20% NaCl, and was able to hydrolyze casein and starch. Strain $M9^T$ had a cell-wall peptidoglycan based on L-Orn-D-Asp, the predominant menaquinone present was menaquinone-7 (MK-7), diaminopimelic acid was not found, and anteiso-$C_{15:0}$ and iso-$C_{15:0}$ were the major fatty acids. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain $M9^T$ belonged to the genus Halobacillus and exhibited 16S rRNA gene similarity levels of 97.8-99.4% with the type strains of the other nine Halobacillus species. The DNA-DNA relatedness of strain $M9^T$ with H. trueperi, the closest relative as regards 16S rRNA gene similarity, and H. locisalis was 21% and 18%, respectively. Therefore, on the basis of phenotypic, genotypic, and phylogenetic data, strain $M9^T$ (=ATCC BAA-$1217^T$, =CIP $108771^T$, =KCTC $3980^T$) should be placed in the genus Halobacillus as a member of a novel species, for which the name Halobacillus blutaparonensis sp. nov. is proposed.

• 한국미생물·생명공학회지
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• 제19권2호
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• pp.153-157
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• 1991

Intracellular enzymes of an extreme halophilic bacterium, Halobacterium sp. HE10, isolated from a saltern in Korea was investigated. The membrane-bound enzyme, NADH dehydrogenase, involved in electron transport system was stimulated by the addition of 2.0 M NaCl. The respiratory enzyme activities such as NADH oxidase and NADH dehydrogenase was decreased on removal of $Na^+$ ion and restored when replaced with cations like $K^+$, $Li^+$and $NH_{4}^{+}$ ions. Furthermore, their activities were affected by the anions such like carbonate, acetate, sulfate, chloride and nitrate at the presence of $Na^+$ion. Lactate dehydrogenase activity was highest at the asturated solution of NaCl and isocitrate dehydrogenase activity was a maximum level at 1.0 M NaCl. These results suggested that the enzyme activites of the respiratory chain in Halobacterium sp. EH10 was stimulated by the presence of $Na^+$ ion.

• 한국수산과학회지
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• 제16권2호
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• pp.68-74
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• 1983

부산 수영만의 조간대 저질에서 moderate halophile인 Listeria denitrificans HB-38을 분리하여 생육에 필요한 NaCl 요구도를 조사한바, 해양환경조건의 배지에서는 $4\%$ NaCl에서 육상환경조건의 배지에서는 $10\%$ NaCl에서 최적농도였으며, 균생육 최적 온도는 $40^{circ}C$였고, 생육최적 pH는 7.5이었다. 그리고 비호염성인 E.coli, KPM 105와 protoplast안정성을 비교해본 결과, 더 높은 stabilizer의 농도를 요구하지는 않았으며, stabilizer로서 NaCl이sorbitol이나 sucrose보다 protoplast에 더 안정성을 주는 것으로 나타났다. HB-38균주의 succinic dehydrogenase 활성에는 NaCl 농도가 증가함에 따라 그 활성 이 증가하다가 $9\%$에서 최대에 달하였다. 반면에 이 호염성효소의 $(NH_4)_2\;SO_4$와 NaCl에 의한 염석은 비호염성인 E.coli 및 bovine liver 세포의 경우보다 더 높은 농도의 염에 의하는 것은 아니였다. 그리고 ethidium bromide에 의한 curing과 agarose gel 전기영동의 결과 HB-38은 extrachromosomal DNA를 가지지 않는 것으로 나타났다.

• 미생물학회지
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• 제29권1호
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• pp.56-62
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• 1991

Extremely halophilic bacteria isolated from salterns at Mado, Kyunggido, Korea, were identified and investigated on their salt requirements. The results have shown that six strains were identified to be belonged to the genus Halobacterium and three strains identified as the fenus Halococcus. Among them, the optimal NaCl concentration for growth of Halobacterium sp. EH10 was at 4.2M and no growth occurs below 2.0M NaCl. The strain, EH10, is nonmotile and showed acid production from glucose, fructose and maltose while H. salinarum is motile and does not produce acid from any carbohydrates. On the other hand, the strain EH10 does not utilize readily glucose while a number of sugars are readily utilized for growth with acid production by H. saccharovorum. Thus, the isolate, EH10, was classified into the genus Halobacterium and could be a novel species of the genus by its main morphological and physiological features including G+C content. The optimal temperature for growth of the isolate, EH10, was 50.deg.C. But this strain did not grow when NaCl was replaced with KCl.

• 한국미생물·생명공학회지
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• 제24권6호
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• pp.671-677
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• 1996

An Extremely halophilic bacterium was isolated from solar salts. The isolated strain was Gram-negative, facultatively anaerobic, and motile bacterium. The colony was circular, smooth, and red-orange color. The strain showed pleomorphism depending on magnesium ion concentrations. The range of temperature and pH for growth of the isolate were 35 -45$\circ$C and 7.0 - 9.0. NaCl concentration for growth of it was 4.3 - 5.0 M. The isolate was catalase and oxidase positive, and sensitive to bacitracin. It showed starch hydrolyzing and acid forming characteristics. DNA G+C content was 62.7 mol%. The morphological, physiological and biochemical characteristics of the isolate resembled those of the Haloarcula vallismortis, therefore it was identified as Haloarcula sp. EH-1.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.309-312
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• 2000

A halophilic bacterium was isolated from domestic marine. The bacterium was gram negative and motile. Transmission electron micrograph after cultivating for 6-, 20-, 72-, and 144 hrs showed that it was bacilli and contained intracellular granules, which were pleomorphic, larger in density by the time and considered to be PHB since they were positive on the sudan black B staining. The presence of sodium chloride was critical, because the isolated marine bacterium could not multiply and even produce any immunostimulant in the deficiency of sodium chloride. The strain produced an immunostimulant, which was investigated for the biological characteristics. The optimal conditions for the production of the immunostimulant were 1 % dextrose and 1 % yeast extract in artificial sea water for carbon and nitrogen sources, respectively. The initial pH and growth temperature for the production were 8.0 and $30^{\circ}C$ under the presence of oxigen, respectively.

• 생명과학회지
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• 제13권4호
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• pp.505-510
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• 2003

멸치 젓갈에서 분리된 파아지의 일반적인 성질과 파아지 DNA 서열을 분석한 결과는 다음과 같다. 멸치 젓갈에서 분리된 파아지의 플라그는 대체로 작고 선명한 핀 형태로 직경 0.5∼l.0 mm이었으며, 파아지의 형태는 지름 68 nm의 대칭형 두부와 길이 100 nm의 contractile 미부를 가지고 있었고 base plate가 관찰되었다. 파아지 DNA의 크기는 약 20 Kbp 정도이었으며, DNA 염기서열은 H. salinarium 파아지 hp 32와 52.87%의 상동성을 나타내었으나 보다 정확한 분류를 위해 파아지 DNA의 전체 염기서열에 대한 분석이 이루어져야 할 것이다.