• 자연과학논문집
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• 제14권1호
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• pp.25-37
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• 2004

T7 RNA 중합효소는 두 종류의 인자 독립형 전사 종결신호를 인지하여 전사연장을 종결하게 되는데 이 중 머리핀 구조가 만들어지지 않는 PHT와 CJ 전사종결 부위는 ‘ATCTGTT' non-template 염기서열 다음에 T염기가 많은 부분이 존재하는 형태로 이루어져 잇다. 이와 같은 전사종결을 민감하게 인지하는 돌연변이 T7 RNA 중합효소인 X4, X19, BG8과 이와 반대로 둔감하게 인지하는 R173C를 제조하여 전사활성도를 측정하였다. 이결과 야생형 T7 RNA 중합효소에 비해 X4는 8%, X19는 33%, BG8은 34%의 전사활성도를 보여 전사활성도가 상당히 감소하는 결과를 보였다. 반면에 R173C는 야생형에 비해 112%의 전사활성도를 보여 야생형과 거의 유사한 전사활성도를 보였다. 또한 PTH 및 CJ 전사종결 부위를 전사주형으로 이용하여 전사하였을 때 X4, X19, BG8은 야생형 RNA중합효소에 비해 종결 부위에서의 종결이 증가하였으나 R173C는 거의 PTH 및 CJ 전사종결부위에서 전사종결이 일어나지 않고 전사가 진행되는 것을 확인하였다.

• 복식
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• 제55권7호
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• pp.142-155
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• 2005

This study shows historical Gagye(가계) The process of change and Characteristics through analysis on the old documents, related papers, and visual data, and represents its meaning in Korean Clothing history through analysis on originality of Korean Gagye(가계) style. Gagye(가계) is the cubic hair style which add other accessories on hair and is divided into two; Gagye(가계) and Chegye(체계) according to its material, role, and function. And Korean Gagye(가계) has been transformed suited to the times and showed various features according to pattern, wearing, and decoration aspect. In its style aspect, Korean Gagye(가계) style shows Hwangye type, Sseugae type, Gogye type, Dagye type, and Braided & coiled hair type. Each shows historical features. In its Wearing aspect, Gagye(가계) style shows Wearing ornamental hairpin on the head, Wearing Rag Ribbon on the head, and Attaching (Detaching) Wearing. And in its Decoration aspect, Gagye(가계) style shows decoration with accessories, one with flower arrangement, and one with shaking.

• Molecules and Cells
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• 제38권11호
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• pp.959-965
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• 2015

Inducible and reversible gene silencing in desired types of cells is instrumental for deciphering gene functions using cultured cells or in vivo models. However, efficient conditional gene knockdown systems remain to be established. Here, we report the generation of an inducible expression system for short hairpin RNA (shRNA) targeted to PTEN, a well-documented dual-specificity phosphatase involved in tumor suppression and ontogenesis. Upon induction by doxycycline (DOX), the reverse tetracycline transcriptional activator (rtTA) switched on the concomitant expression of GFP and a miR-30 precursor, the subsequent processing of which released the embedded PTEN-targeted shRNA. The efficacy and reversibility of PTEN knockdown by this construct was validated in normal and neoplastic cells, in which PTEN deficiency resulted in accelerated cell proliferation, suppressed apoptosis, and increased invasiveness. Transgenic mice harboring the conditional shRNA-expression cassette were obtained; GFP expression and concurrent PTEN silencing were observed upon ectopic expression of rtTA and induction with Dox. Therefore, this study provides novel tools for the precise dissection of PTEN functions and the generation of PTEN loss of function models in specific subsets of cells during carcinogenesis and ontogenesis.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
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• pp.185.2-185.2
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• 2016

The plasma density is an essential plasma parameter describing plasma physics. Furthermore, it affects the throughput and uniformity of plasma processing (etching, deposition, ashing, etc). Therefore, a novel technique for plasma density measurement has been attracting considerable attention. Microwave probe is a promising diagnostic technique. Various type of cutoff, hairpin, impedance, transmission, and absorption probes have been developed and investigated. Recently, based on the basic type of probes, modified flat probe (curling and multipole probes), have been developing for in situ processing plasma monitoring. There is a need for comparative study between the probes. It can give some hints on choosing the reliable probe and application of the probes. In this presentation, we make attempt of numerical study of different kinds of microwave probes. Characteristics of frequency spectrum from probes were analyzed by using three-dimensional electromagnetic simulation. The plasma density, obtained from the spectrum, was compared with simulation input plasma density. The different microwave probe behavior with changes of plasma density, sheath and pressure were found. To confirm the result experimentally, we performed the comparative experiment between cutoff and hairpin probes. The sheath and collision effects are corrected for each probe. The results were reasonably interpreted based on the above simulation.

• 한국전자파학회논문지
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• 제18권5호
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• pp.547-552
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• 2007

본 논문에서는 두 마이크로스트립 라인으로 이루어진 헤어핀 형태의 결합 선로를 이용하여 특성이 개선된 링 하이브리드를 설계하였다. 제안한 구조는 링 하이브리드의 $\lambda/4$ 라인에 헤어핀 형태의 결합 선로를 삽입하여 두 선로가 가까워짐에 따라 선로간의 커플링으로 세 개의 감쇠극이 생겨 넓은 저지대역을 갖는 결합 선로 구조를 이용하여 구성함으로써 링 하이브리드의 크기 감소뿐만 아니라 고조파 제거 특성도 부가하였다. 중심 주파수 2.55 GHz에서 일반적인 링 하이브리드와 특성이 같고 3차 고조파를 포함한 12 GHz까지 -20 dB 이하의 고조파 저지 특성을 가지고 있다. 또한 링 부분의 크기는 기존 링 하이브리드의 1/4 크기로 줄어들었다. 제작된 링 하이브리드의 측정된 주파수 특성 결과는 시뮬레이션 결과와 잘 일치한다.

• 복식문화연구
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• 제9권5호
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• pp.712-722
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• 2001

The purpose of this study is to classify of the Hair ornaments in old tombs of the Three Kingdom States. Hair ornaments was divided into Combs, Rod-like hairpins, Chae[;釵], Boyo[;步搖] Combs were 2 type in according to the tooth's space, wide-tooth comb[;梳] and fine-tooth comb(;比). The general name of two type was called Jeul(;櫛). Combs were excavated from the only ancient Silla tombs. Rod-like hairpins were called Gha(叉) or Jam(:簪). They were found in most of the old tombs in Goauryeo, Baekje, ancient Silla tombs. The style was L-type and embellished circular head-type. Chae(;釵) was understand Cha(:叉) that had two tongs. Chae(:釵) was generally U-type and rarely hairtweezers -type. Jakchae(;爵釵) was understand distinctive Chae(;釵) hanged with phoenix. This type was excavated from the only Baekje tombs of King of Muryeong. Boyo(;步搖) was originally a hair decoration of the northern nomadic tribes which had been introduced to the Wijin period. Boyo gained its popularity by the women in Dang Dynasty, hair ornaments were considered indicators of status and wealth. Boyo(;步搖) hair accessory features of which are suspended from a elaborate hairpin structure. Such pieces were designed to sway as their wearer walked. The bequest was not found, but we catch the Boyo(; 步搖) that was women'head decoration in painting of old tombs in Goguryeo.

• 한국ITS학회 논문지
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• 제6권1호
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• pp.38-46
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• 2007

본 논문에서는 평면형 MMIC 공진기를 이용하여 X대역에서 동작하는 낮은 위상잡음 특성의 HBT 발진기를 설계하였다. 제안한 공진기는 기존의 분포소자를 이용한 헤어핀 공진기 면적의 1/4 수준으로 크기를 소형화를 하였다. 발진기의 측정결과 발진주파수는 8.295 GHz, 출력전력은 4.8 dBm, 위상잡음은 100 kHz 옵셋 주파수에서 -106.8 dBc/Hz와 1 MHz 옵셋 주파수에서 -121.7 dBc/Hz를 보였다.

• Wind and Structures
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• 제10권2호
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• pp.135-151
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• 2007

This paper examines the extreme gust profiles obtained by conditionally sampling full-scale velocity data obtained in the lower part of the atmospheric boundary layer. It is demonstrated that three different types of behaviour can be observed in the streamwise component of velocity. In all cases the corresponding vertical velocity component illustrates similar behaviour. An idealised horseshoe vortex model and a downburst model are investigated to examine if such structures can explain the behaviour observed. In addition, an empirical model is developed for an isolated gust corresponding to each of the three types of behaviour observed. It is possible that the division of the gust profile into three different types may lead to an improvement in the correlation of extreme gust events with respect to type.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1999년도 하계학술대회 논문집 D
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• pp.1852-1854
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• 1999

In order to enhance satellite communication system performance, filters are required with the characteristics of sharp skirt, low insertion loss, and high power handling capability. But the performance of microwave passive filters is significantly declined by the conduction losses, especially in case of planar structures using film conductors. By using high temperature superconducting(HTS) film material as the conductor, higher performance could be expected. We have designed and developed narrow bandpass filters using haripin-type superconducting microstrip line for satellite communication. High quality superconducting YBCO thin films have been grown on MgO substrates by pulsed laser deposition(PLD) The deposited YBCO films were patterned by conventional wet-etching process. The transition temperatures of these films had shown 86 - 89 K. The film thicknesses were about 500 nm. Experimental results are presented for the insertion loss and return loss of the filter at 60 K.

• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.72-76
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• 2017

Detection of exon 19 deletion mutation in the epidermal growth factor receptor (EGFR) gene, which results in increased and sustained phosphorylation of EGFR, is important for diagnosis and treatment guidelines in non-small-cell lung cancer. Here, we have developed a simple and convenient detection system using the interaction between G-quadruplex and fluorophore thioflavin T (ThT) for discriminating EGFR exon 19 deletion mutant DNA from wild type without a label and quencher. In the presence of exon 19 deletion mutant DNA, the probe DNAs annealed to the target sequences were transformed into G-quadruplex structure. Subsequent intercalation of ThT into the G-quadruplex resulted in a light-up fluorescence signal, which reflects the amount of mutant DNA. Due to stark differences in fluorescence intensity between mutant and wild-type DNA, we suggest that the induced G-quadruplex structure in the probe DNA can report the presence of cancer-causing deletion mutant DNAs with high sensitivity.