• Asian Pacific Journal of Cancer Prevention
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• 제15권14호
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• pp.5577-5582
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• 2014

Objective: To observe the effects of metastasis-associated tumor gene family 2 (MTA2) depletion on human breast cancer cell proliferation and metastasis. Methods: A short-hairpin RNA targeting MTA2 was chemically synthesized and transfected into a lentivirus to construct Lv-shMTA2 for infection into the MDA-MB231 human breast cancer cell line. At 48 hours after infection cells were harvested and mRNA and protein levels of MTA2 were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting, respectively. Cell viability and metastasis were assessed by CCK-8, wound-healing assay and Transwell assay, respectively. In addition, a xenograft model of human breast cancer was constructed to investigate cancerous cell growth and capacity for metastasis. Results: After infection with Lv-shMTA2, mRNA and protein levels of MTA2 was significantly reduced (p<0.05) and MDA-MB231 cell proliferation and metastasis were inhibited (p<0.05). In addition, mean tumor size was smaller than that in control group nude mice (p<0.05) and numbers of metastatic deposits in lung were lower than in control group mice (p<0.05). Depletion of MTA2 affected MMP-2 and apoptosis-related protein expression. Conclusions: For the first time to our knowledge we showed that MTA2 depletion could significantly inhibit human breast cancer cell growth and metastasis, implying that MTA2 might be involved in the progression of breast cancer. The role of MTA2 in breast cancer growth and metastasis might be linked with regulation of matrix metalloproteinase and apoptosis.

• 한국전자파학회논문지
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• 제18권10호
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• pp.1126-1135
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• 2007

본 논문에서는 기존에 제안된 소형화 기법을 응용하여 단일 제어 전압을 갖는 소형화된 튠어블 필터를 제안하였다. 기존에 제안된 소형화 기법은 양쪽이 접지된 구조와 캐패시터를 이용하여 임의의 평행 결합 선로 필터를 매우 간단하게 소형화 할 수 있다. 이때 각 단에서 소형화를 위해 사용되는 캐패시턴스의 값이 소형화된 길이에 상관없이 항상 동일한 비를 유지하는 것을 이용하여 단일 제어 전압을 가지며, 동일한 특성을 유지하는 튠어블 필터를 제안하였다. 제안된 기법을 검증하기 위해 900 MHz 대역의 0.5 dB 리플을 갖는 3단 체비셰프(Chebyshev) 필터를 튠어블 필터로 제작하였다. 제작은 Toshiba사의 1SV277 가변 용량 다이오드를 이용하여 타코닉사의 CER-10 기관에 구현하였다. 제어 전압이 $0.5{\sim}4V$로 변함에 따라 필터의 중심 주파수가 $606{\sim}944MHz$로 338 MHz 변하였다. 제안된 튠어블 필터는 제어 전압이 증가함에 따라 손실이 증가하며, 대역폭이 약간 변하는 것을 제외하고 본래의 특성을 잘 유지하였다.

• 한국전자파학회논문지
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• 제18권2호
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• pp.126-135
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• 2007

본 논문은 기존에 제시된 집중 소자 캐패시터와 접지를 이용한 평행 결합 선로 필터의 소형화 기법이 갖는 대역폭 감소 문제를 해결하는 기법을 제안하였다. 평행 결합 선로 필터는 그 설계 및 제작이 쉬워 RF(Radio Frequency) 필터로 많은 응용이 이루어지고 있다. 이러한 평행 결합 선로 필터에 대하여 기존에 제시된 집중 소자 캐패시터와 접지를 이용한 소형화 기법은 적은 수의 캐패시터만을 이용하여 필터를 소형화할 수 있으며, 더불어 고조파 특성의 개선 및 스컷 특성의 개선 등의 부가적인 장점이 있는 기법이나 제시된 기법을 이용하여 필터를 소형화할 경우 대역폭이 감소한다는 문제점을 가지고 있었다. 본 논문에서는 이러한 대역폭의 감소를 필터를 구성하는 각 단의 평행 결합 선로의 군지연 변화를 계산하여 대역폭의 감소의 정도를 유추하고, 역으로 대역폭이 감소하는 만큼 사전에 필터의 대역폭을 크게 설계함으로 소형화로 인한 대역폭의 감소를 해결하는 방법을 제시하였다. 제안된 기법에 대한 검증을 위해 테프론(${\varepsilon}_r=2.2$) 기판을 사용하여 무선 랜 대역인 5.2 GHz대역의 FBW(Fractional Band Width) 10%의 필터를, 제안한 기법을 적용하여 공진기의 길이를 ${\lambda}/4$로 줄인 헤어핀 형태로 제작 및 측정하여 제안된 기법의 타당성을 확인하였다.

• 한국전자파학회논문지
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• 제18권5호
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• pp.547-552
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• 2007

본 논문에서는 두 마이크로스트립 라인으로 이루어진 헤어핀 형태의 결합 선로를 이용하여 특성이 개선된 링 하이브리드를 설계하였다. 제안한 구조는 링 하이브리드의 $\lambda/4$ 라인에 헤어핀 형태의 결합 선로를 삽입하여 두 선로가 가까워짐에 따라 선로간의 커플링으로 세 개의 감쇠극이 생겨 넓은 저지대역을 갖는 결합 선로 구조를 이용하여 구성함으로써 링 하이브리드의 크기 감소뿐만 아니라 고조파 제거 특성도 부가하였다. 중심 주파수 2.55 GHz에서 일반적인 링 하이브리드와 특성이 같고 3차 고조파를 포함한 12 GHz까지 -20 dB 이하의 고조파 저지 특성을 가지고 있다. 또한 링 부분의 크기는 기존 링 하이브리드의 1/4 크기로 줄어들었다. 제작된 링 하이브리드의 측정된 주파수 특성 결과는 시뮬레이션 결과와 잘 일치한다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1999년도 하계학술대회 논문집 D
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• pp.1852-1854
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• 1999

In order to enhance satellite communication system performance, filters are required with the characteristics of sharp skirt, low insertion loss, and high power handling capability. But the performance of microwave passive filters is significantly declined by the conduction losses, especially in case of planar structures using film conductors. By using high temperature superconducting(HTS) film material as the conductor, higher performance could be expected. We have designed and developed narrow bandpass filters using haripin-type superconducting microstrip line for satellite communication. High quality superconducting YBCO thin films have been grown on MgO substrates by pulsed laser deposition(PLD) The deposited YBCO films were patterned by conventional wet-etching process. The transition temperatures of these films had shown 86 - 89 K. The film thicknesses were about 500 nm. Experimental results are presented for the insertion loss and return loss of the filter at 60 K.

• Asian Pacific Journal of Cancer Prevention
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• 제16권6호
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• pp.2341-2345
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• 2015

This study aimed to investigate the relationship between prognosis and protein and mRNA expression of an apoptotic inhibitor gene, survivin, in patients with nasopharyngeal carcinoma. Furthermore, functions of the survivin gene in the CNE2 nasopharyngeal carcinoma cell line were assessed. Immunohistochemistry and in situ hybridization were used in detecting the survivin protein and mRNA in 44 nasopharyngeal carcinoma specimens, and 30 chronic nasopharyngitis samples as controls. Survivin gene expression in CNE2 cell line was suppressed with an shRNA (short hairpin RNA). The positive ratios of expression for survivin protein and mRNA in nasopharyngeal carcinoma were 79.5% and 75.0% respectively, obviously higher than in the control group (p<0.01), and there is very good consistency between the two methods. The mean survival time of patients with higher survivin protein or mRNA expression was shorter than in patients with lower levelsv(p<0.01). Proliferation of the CNE2 cell line was distinctly inhibited by the shRNA. The results indicate that overexpression of the survivin gene plays an important role in onset and development of nasopharyngeal carcinoma, and it may be helpful for prognostic appraisal.

• Biomolecules & Therapeutics
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• 제27권6호
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• pp.553-561
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• 2019

Rab25, a member of the Rab11 small GTPase family, is central to achieving cellular polarity in epithelial tissues. Rab25 is highly expressed in epithelial cells of various tissues including breast, vagina, cervix, the gastrointestinal tract, and skin. Rab25 plays key roles in tumorigenesis, mainly by regulating epithelial differentiation and proliferation. However, its role in skin physiology is relatively unknown. In this study, we demonstrated that Rab25 knock-out (KO) mice show a skin barrier dysfunction with high trans-epidermal water loss and low cutaneous hydration. To examine this observation, we investigated the histology and epidermal differentiation markers of the skin in Rab25 KO mice. Rab25 KO increased cell proliferation at the basal layer of epidermis, whereas the supra-basal layer remained unaffected. Ceramide, which is a critical lipid component for skin barrier function, was not altered by Rab25 KO in its distribution or amount, as determined by immunohistochemistry. Notably, levels of epidermal differentiation markers, including loricrin, involucrin, and keratins (5, 14, 1, and 10) increased prominently in Rab25 KO mice. In line with this, depletion of Rab25 with single hairpin RNA increased the expression of differentiation markers in a human keratinocyte cell line, HaCaT. Transcriptomic analysis of the skin revealed increased expression of genes associated with skin development, epidermal development, and keratinocyte differentiation in Rab25 KO mice. Collectively, these results suggested that Rab25 is involved in the regulation of epidermal differentiation and proliferation.

• 한국콘텐츠학회논문지
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• 제6권4호
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• pp.1-10
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• 2006

본 논문에서는 2.4GHz 대역에서의 응용을 위한 광대역 RF 모들을 설계하고 제작하였다. 무선 주파 신호를 중간 주파수로 변환하기 위한 RF 모듈은 3단 증폭기로 이루어진 저잡음 증폭기(LNA), 단종단 게이트 믹서, 정합 회로, 헤어핀 라인 대역 통과 필터, 쳬비셰프 저역 통과 필터로 구성하였다. 저잡음 증폭기는 높은 이득과 안정도를 갖도록 설계하였으며, 단종단 게이트 믹서는 높은 변환이득과 넓은 동작 영역을 갖도록 설계하였다. 광대역 RF모듈의 해석에서는 복합화된 하모닉 밸런스드 기법을 사용하여 RF모듈의 동작 특성을 해석하였다. 설계된 RF 모듈은 55.2dB의 변환이득, 1.54dB의 낮은 잡음 특성, $-120{\sim}-60dBm$의 넓은 RF전력 동작 영역, -60dBm의 낮은 고조파 성분 그리고 RF, IF, LO포트 간에 우수한 분리 특성을 갖는다.

• 대한간호학회지
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• 제5권1호
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• pp.79-86
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• 1975

It had been thought the role of Nursing administrator was still not started along the right line in Korea because of the Lacking of full understanding and recognition of the real meaning of administrator's role, and because there are several Rinds of problems and difficulties in actual role. The present study is an attempt to clarify some existing relationships between the chief Nurse's Leadership style and the organizational climate of Hospitals. The problems of the study are specifically started as follows ; 1) What influence does the individual behavior have on the formation of the organizational climate of Hospital? 2) From what do the difference of climate arise? 3) How are the individual be heavier and organizational climate of Hospital measured ? In order to see the relationships or the interactions between the two factors, the chief nurse's Leadership style and organizational climate of hospital , the researcher has classified. He former into; 1) effective Leadership style. 2) initiating structure leader ship style. 3) Consideration structure Leadership style, and 4) ineffective Leadership style, and the latter in to. 1) open climate and 2) closed climate The Chief Nurses Leadership style has been. Classified into consideration-human relations approaches and initiating structure approaches according to L. B. D. Q by Hapin. Organizational climate of hospital has been classified into open-closed continuum according to O. C. H Q. by Hairpin and Croft. The results obtained are as follows : 1) The chief nurse's Leadership style is closer to Initiating structure Leadership style than Consideration structure lure Leadership style. 2) The organizational climate of hospital is closer to open climate than closed climate. 3) The chief nurse's Leadership sty]e and the organizational climate of hospital to not show any significant relations.

• Molecules and Cells
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• 제37권12호
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• pp.857-864
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• 2014

Astrocyte elevated gene-1 (AEG-1) is a recently discovered oncogene that has been reported to be highly expressed in various types of malignant tumors, including renal cell carcinoma. However, the precise role of AEG-1 in renal cancer cell proliferation and apoptosis has not been clarified. In this study, we transfected the renal cancer cell line Caki-1 with a plasmid expressing AEG-1 short hairpin RNA (shRNA) and obtained cell colonies with stable knockdown of AEG-1. We found that AEG-1 down-regulation inhibited cell proliferation and colony formation and arrested cell cycle progression at the sub-G1 and G0/G1 phase. Western blot analysis indicated that the expression of proliferating cell nuclear antigen (PCNA), cyclin D1 and cyclin E were significantly reduced following AEG-1 down-regulation. In addition, AEG-1 knockdown led to the appearance of apoptotic bodies in renal cancer cells, and the ratio of apoptotic cells significantly increased. Expression of the antiapoptotic factor Bcl-2 was dramatically reduced, whereas the pro-apoptotic factors Bax, caspase-3 and poly (ADPribose) polymerase (PARP) were significantly activated. Finally, AEG-1 knockdown in Caki-1 cells remarkably suppressed cell proliferation and enhanced cell apoptosis in response to 5-fluorouracil (5-FU) treatment, suggesting that AEG-1 inhibition sensitizes Caki-1 cells to 5-FU. Taken together, our data suggest that AEG-1 plays an important role in renal cancer formation and development and may be a potential target for future gene therapy for renal cell carcinoma.