• 제목/요약/키워드: hair growth activity

검색결과 118건 처리시간 0.03초

익기보혈탕(益氣補血湯)이 C57BL/6 마우스의 육모(育毛)촉진 및 Cytokine 변화에 미치는 영향 (Effect of Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-tang) on Hair Regrowth and Cytokine Changes on Hair-Removed C57BL/6 Mice)

  • 홍정애;송미연;최인화;손락원;정석희
    • 대한한의학회지
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    • 제31권1호
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    • pp.138-152
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    • 2010
  • Objective: Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-t$\bar{a}$ng) is one of the representative prescriptions for invigoration of vitality and nourishing of the blood. This study was carried out to investigate the effects of Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-t$\bar{a}$ng) on hair regrowth and cytokine changes in a shaving model of C57BL6 mice. Method: Five-week-old mice were acclimated for 1 week at a temperature between $21-23^{\circ}C{\acute{E}}$, 40-60% relative humidity, and 12h of a light/dark cycle before beginning of the experiment. There were two groups including normal saline (control) and a positive control of oral intake of Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-t$\bar{a}$ng) extract (sample) in 18 female mice. The test compounds were topically treated once a day over 14 days. The hair regrowth was photographically and histologically determined during the experimental period of 14 days. Revelation of TGF-${\beta}1$ and EGF were also determined using immunohistochemistry. In addition to that, IFN-$\gamma$, IL-4 and IL-10 were determined in serum. Results: Hair regrowth in the sample group was promoted earlier and faster than the control group, as shown by concentrations of hairs and thick-hair ratio in the sample group. TGF-${\beta}1$ was not revealed in either control or sample group. EGF was strongly positive in out root sheath of some thick hair of the sample group. Serum IFN-$\gamma$ was significantly decreased in the sample group compared with the control group at 7 experimental days. However, it was not significantly decreased at 14 experimental days. Serum IL-4 was significantly increased in the sample group compared with the control group at 7 experimental days. However, it was not significantly decreased at 14 experimental days. Serum IL-10 was decreased in the sample group compared with the control group, but with no real statistical significance. Conclusion: These results suggest that Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-t$\bar{a}$ng) has hair growth promoting activity and it can be used for treatment of alopecia. Also, these effects relate to EGF revelation of hair roots, a decrease in serum IFN-$\gamma$, and an increase of serum IL-4.

칸나비디올(CBD)의 항산화 활성 및 인간 모유두 세포 증식에 미치는 영향 (Antioxidant Activity of Cannabidiol (CBD) and Effect on Its Proliferation in Human Dermal Papilla Cells)

  • 김수현;심규상;천정윤;장재웅;정수진;서예희;안혜명;송봉근;권기석;이중복
    • 생명과학회지
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    • 제33권3호
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    • pp.234-241
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    • 2023
  • 최근 세계 여러 나라에서 대마초 및 대마제품을 합법화하고 대마를 이용한 다양한 치료법에 대한 연구가 활발히 진행되고 있다. 그러나 대마에는 생물학적 효과가 아직 확립되지 않은 여러 화합물들이 포함되어 있다. 본 연구에서는 인간 모유두 세포(HDPC)의 모발 성장에 대한 칸나비디올(CBD)의 효과를 조사하였다. 2,2'-Azino-bis (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) 및 2,2-diphenyl-1-picrylhydrazyl (DPPH) 라디칼 소거 분석법을 활용하여 CBD의 항산화 활성을 측정하였다. 모유두 세포에서 CBD의 세포생존률은 WST-1 분석법으로 측정하였다. CBD 처리에 의한 모유두 세포에서 모발 성장과 관련된 인자의 발현은 real-time PCR 및 western blot으로 측정하였다. CBD의 항산화 활성 측정결과, DPPH 및 ABTS 자유 라디칼 소거 활성의 IC50 값은 각각 15.46±0.24 μM 및 13.90±0.06 μM으로 뛰어난 활성 산소 제거능을 나타냈다. CBD 처리군은 대조군에 비해 세포 증식이 증가하는 경향을 나타냈다. 또한 모유두 세포에서 Real-Time PCR과 Western blotting을 통해 모발 성장 관련 인자를 측정한 결과, CBD 처리로 인하여 성장 관련 인자들이 증가하는 것으로 나타났다. 종합적으로, 항산화 활성이 높은 CBD는 모유두 세포에서 세포 증식을 증가시키고 모발 성장 관련 인자들을 긍정적으로 조절합니다. 이러한 결과는 CBD가 탈모증에 대한 잠재적으로 활용될 수 있음을 시사한다.

Paraquat에 의해 유도된 Superoxide Dismutase 결핍 효모의 산소 독성 (Oxygen Toxicity of Superoxide Dismutase-Deficient Saccharomyces cerevisiae by Paraquat)

  • 김지면;남두현용철순허근
    • KSBB Journal
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    • 제10권5호
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    • pp.561-567
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    • 1995
  • 활성산소 소거 효소인 superoxide dismutase (SOD)가 결핍된 효소 변이주들을 대상으로 하여, 활성산소 유발물질인 paraquat을 배지에 첨가하여 배양하면서 산소 독성을 관찰하였다. 호기 상태에서 는 MnSOD (mitochondria SOD) 결핍 효모는 CuZnSOD (세포질 SOD) 결핍 효모보다 성장이 많 이 저하되었지만, 혐기 상태에서는 이들 SOD 결핍 효모 모두 성장 속도에서 야생 효모와 큰 차이를 보이지 않았다. Paraquat으로 처 리한 결과, 호기 배양 에서 CuZnSOD 결핍 효모는 O.OlmM 이상에서 성 장하지 않음을 알 수 있었다. 따라서 O.OOlmM paraquat을 배지에 첨가한 후 호기적으로 배양하면서 세포내 성분의 변화를 관찰하였더니, CuZnSOD 결핍 효모에셔는 catalase 역가가 떨어진 반면 glutathion peroxidase 역가와 세포막 지질의 과산 화물이 증가하였으며, MnSOD 결핍 효모에서는 catalase 역가와 glutathion peroxide 역가가 모두 조금씩 증가하면서 세포막 지질의 과산화물은 그다지 변화하지 않았다. 이러한 사실로부터 CuZnSOD 가 없는 경우 활성산소 소거계로써 catalase 보다 glutathion peroxidase가 훨씬 활성화되어지지만, 이렇게 활성화된 glutathion peroxidase로는 세포질내 산소 radical을 완벽히 제거하는데에는 다소 불충분 하다는 사실을 알 수 았었다. 한편, 혐기적 배양에서 는 SOD 결핍 효모들의 catalase 역가는 모두 감소 한 반면, glutathion peroxidase 역가는 다소 증가하였고, 또한 세포막 지질의 과산화물은 다소 감소하는 추세를 보였다. 이는 혐기 상태에서 산소 radical 이 소량밖에 생기지 않으므로, 활성화된 glutathion p peroxidase에 의해 어느 정도 극복되어지기 때문인 것으로 사료된다. 두 집단 간에 어느 정도 방향성은 있는 것으로 판단된다.ds for eagerness for lasting life not only in this world but also in the other world., Keun-Kap, Scarf, Pee-Bak, Hung-Kap, Pok-Kap, Yang-Dang-Kap, We-Yo-Kap, Kum-Kap, and caries arms. Lower-level officials wore Pe-Bal, Kun-Mo, gae, won-leung, very small sleve jacket, a long coat reaching up to the knee length, slacks, belt, loin cloth and apron. 5) Children's bind their hair up angle shape and wore a half long jacket raching up to the hip and slacks. 나) Women's wear; 1. hair style and hair dress; 1) High rank women's hair style was very extravaganceful. They made their hair top knot (one, two, or more knots) and decoraed precious stone, pan shape head dress, wheel shape head dress, and flower shape precious stone decorated head dress. 2. Clothes ; 1) High rank ladi's wore Kun-Kyun attached jacket, and jacket sleeves decorated pleats, and pleats decorated long skirt, apron, back apron, knot belt, scarf, this type is the same

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Isolation and Antimicrobial Activity of Dichlororinated Bibenzyl Compound

  • Na, Young-Soon;Lee, Jae-Sook;Baek, Seung-Hwa
    • 동의생리병리학회지
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    • 제21권1호
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    • pp.231-234
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    • 2007
  • Dichlororinated bibenzyl compound (4) has been isolated from the New Zealand liverwort. This compound was elucidated using 1D/2D-NMR and mass spectral method. The compound (3) inhibited the growth of the Gram positive bacterium Bacillus subtilis ATCC 19659, (2 mm inhibition zone and 2 mm inhibition zone at 30 ${\mu}$g/disc), Candida albicans ATCC 14053, (2 mm inhibition zone and 2 mm inhibition zone at 30 ${\mu}$g/disc), and the dermatophytic fungi Trichophyton mentagrophytes ATCC 28185, (12 mm inhibition zone at 30 ${\mu}$g/disc) and Cladosporium resinae ATCC 52833 (2 mm inhibition zone at 30 ${\mu}$g/disc). This bibenzyl compound (4) exhibited antimicrobial activity.

부챗말 Glucopyranosyldiacylglycerol의 탈모방지 효능 (The Effect of Glucopyranosyldiacylglycerol from Padina arborescens on the Prevention of Hair-Loss)

  • 강정일;김정은;김상철;한상철;이지혁;이재현;노의준;전유진;유은숙;강희경
    • 생약학회지
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    • 제51권1호
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    • pp.41-48
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    • 2020
  • This study was conducted to evaluate the effects of Padina arborescens and 1-O-myristoyl-2-O-oleoyl-3-O-(α-D-glucopyranosyl)-glycerol(MOGG), its active component, on the prevention of hair loss. The P. arborescens extract and MOGG inhibited the activity of 5α-reductase, which converts testosterone to dihydrotestosterone(DHT), a main cause of androgenetic alopecia. When immortalized rat vibrissa dermal papilla cells were treated with MOGG, the proliferation of dermal papilla cells significantly increased. In addition, we found that the P. arborescens extract and MOGG could open the KATP channel, which may contribute to increase hair growth. Furthermore, MOGG promoted PGE2 production in HaCaT cells. The results suggest that MOGG from the P. arborescens extract has the potential to treat alopecia via 5α-reductase inhibition, the proliferation of dermal papilla, the opening of the KATP channel and/or increase of PGE2 production.

곡물과 적하수오를 혼합한 에탄올 추출물이 멜라닌 합성에 미치는 영향 (Effect of Ethanolic Extracts Mixed with Grains and Fallopia multiflora on Melanogenesis)

  • 이은빈;김문무
    • 생명과학회지
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    • 제29권4호
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    • pp.461-469
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    • 2019
  • 본 연구의 목적은 흑모 성장과 관련 있는 천연물을 개발하기 위하여 흑미, 흑임자, 현미, 서목태 및 적하수오의 에탄올 추출혼합물의 melanin 합성에 대한 효능을 조사한 것이다. 흑모가 백모로 변화되는 원인으로 $H_2O_2$가 모낭 주위에 축적되어 melanin 합성이 저하되어 백모가 유발되는 것으로 보고된 바 있다. MIXEE는 DPPH radical에 대한 소거능력과 활성산소에 대한 환원력이 있는 것으로 관찰되었다. 뿐만 아니라 세포내 $H_2O_2$ 수준을 감소시켰는데, 이러한 결과는 MIXEE는 산화를 억제하여 $H_2O_2$에 의하여 감소된 melanin 합성을 촉진 시킬수 있다는 것을 나타내었다. 한편 in vitro에서 MIXEE는 tyrosinase 활성에는 영향을 주지 않았지만 L-dopa에서 melanin 합성을 촉진하였다. 더욱이, B16F1세포에서 MIXEE는 melanin 생성을 세포수준에서 촉진하였다. Western blot을 이용한 단백질 발현 분석에서 MIXEE는 SOD-2 및 SOD-3. 특히 DOPA에서 melanin 이 생성되는 과정에 관여하는 효소인 TRP-2 발현에 긍정적인 영향을 미치는 것으로 입증되었다. 이상의 결과는 MIXEE가 백발화과정을 가역화시키는 과정에서 주요한 역할을 하는 melanin 생성을 촉진하는 하나의 약학 소재로 응용될 수 있다는 것을 시사하고 있다.

Pharmacological Screening of Crude Extracts from Medicinal Plants (II)

  • Yook, Chan-Nam;Lee, Jae-Sug;Baek, Seung-Hwa
    • 동의생리병리학회지
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    • 제21권4호
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    • pp.1004-1009
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    • 2007
  • The effects of crude extracts from medicinal plants on biological activity were investigated. The crude ethanol extract of H. paucistipula inhibited the growth of the Gram positive bacterium Bacillus subtilis ATCC 19659, (2 mm inhibition zone at 150 ${\mu}g$/disc) and the dermatophyte Trichophyton mentagrophytes ATCC 28185, (7 mm inhibition zone at 150 ${\mu}g$/disc), and toxic to P388 murine leukaemia cells ($IC_{50}\;2.48\;{\mu}g/ml$ at 75 ${\mu}g$/disk). This crude ethanol extract of H. paucistipula is the strongest antimicrobial and cytotoxic activities against P388 murine leukaemia cells (ATCC CCL 46 P388D1).

Antifungal Activity of the Crude Extract from Quintinia acutifolia on the Dermatophytic fungus

  • Lee, Jae-Sook;Chung, Jong-Gab;Oh, Hyun-Ju;Na, Young-Soon;Baek, Seung-Hwa
    • 동의생리병리학회지
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    • 제19권2호
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    • pp.508-510
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    • 2005
  • The crude extract of Quintinia acutifolia Kirk inhibited the growth of the Gram positive bacterium Bacillus subtilis ATCC 19659, (3 mm inhibition zone at $150\;{\mu}g/disc$) and the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185, (3 mm inhibition zone at $150\;{\mu}g/disc$), and cytotoxic to P388 murine leukaemia cells ATCC CCL 46 P388D1, ($IC_{50}$ $50,000\;{\mu}g/mL$ at $150\;{\mu}g/disc$). However, Candida albicans (ATCC 14053) did not observed the antimicrobial activity and the cytotoxic activity to BSC monkey kidney cells ($({\alpha})$ 5 mg/mL, $150\;{\mu}g/disc$).

Preventable effect of L-threonate, an ascorbate metabolite, on androgen-driven balding via repression of dihydrotestosteroneinduced dickkopf-1 expression in human hair dermal papilla cells

  • Kwack, Mi-Hee;Ahn, Ji-Sup;Kim, Moon-Kyu;Kim, Jung-Chul;Sung, Young-Kwan
    • BMB Reports
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    • 제43권10호
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    • pp.688-692
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    • 2010
  • In a previous study, we recently claimed that dihydrotestosterone (DHT)-inducible dickkopf-1 (DKK-1) expression is one of the key factors involved in androgen-potentiated balding. We also demonstrated that L-ascorbic acid 2-phosphate (Asc 2-P) represses DHT-induced DKK-1 expression in cultured dermal papilla cells (DPCs). Here, we investigated whether or not L-threonate could attenuate DHT-induced DKK-1 expression. We observed via RT-PCR analysis and enzyme-linked immunosorbent assay that DHT-induced DKK-1 expression was attenuated in the presence of L-threonate. We also found that DHT-induced activation of DKK-1 promoter activity was significantly repressed by L-threonate. Moreover, a co-culture system featuring outer root sheath (ORS) keratinocytes and DPCs showed that DHT inhibited the growth of ORS cells, which was then significantly reversed by L-threonate. Collectively, these results indicate that L-threonate inhibited DKK-1 expression in DPCs and therefore is a good treatment for the prevention of androgen-driven balding.

고려엉겅퀴(Cirsium setidens (Dunn) Nakai)의 구성성분 및생리활성에 관한 리뷰 (A review on Phytochemistry and pharmacological Activities of Cirsium setidens (Dunn) Nakai)

  • 조미애;김범정
    • 대한본초학회지
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    • 제38권4호
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    • pp.31-43
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    • 2023
  • Objectives : The objective of this study was to investigate the phytochemistry and pharmacological activities of Cirsium setidens. Methods : Domestic and international articles about Cirsium setidens were investigated. A review was perfoemed via DB searching engine such as Sci.Direct, Springer, DBpia, KISS, Google scholar, Kipris, and so on. Total 73 listed literature were classified by compound analysis and pharmacological efficacy. Results : C. setidens contains pectolinarin and its glycoside, pectolinarigenin as index compounds, and linarin, apigenin, diosmetin, scopoletin, acacetin, cirsimarin, cirsimaritin, setidenosides A and B, silymarin, hispidulin, 92 volatile compounds, and 15 fatty acids. The Pharmacological activities of C. setidens has been reported to inhibit of platelet aggregation and fat accumulation in the liver, inhibit to hepatitis, anti-cancer, antibacterial, skin improvement, hair growth, liver protection, anti-diabetic, anti-inflammatory, sedative. Also, It has been reported the effect of cholesterol-lowering and anti-obesity, neuroprotective effects, increasing human stem cell viability, inhibiting osteoclast formation and osteogenic differentiation. Conclusion : This reviews showed that C. setidens which has been traditionally used for the treatment of inflammation and hypertension, has anticancer and river protective effect, as well as hair loss and diet. In order to maximize the efficacy of C. setidens, research has also begun on the effect of processing processes such as fermentation or fine powdering and combining natural plant resources.