• 한국가금학회지
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• 제10권1호
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• pp.60-66
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• 1983

Monoclonal antibodies against Taka virus, a variant of Newcastle disease virus (NDV), were produced to compare the antigenicites of several avian paramyxoviruses including NDV. It was also used to study the activesite(s) of haemagglutin (HA) and neuraminidase activities of NDV. Five independent hybrid cell lines, which produced monoclonal antibodies against haemagglutinin-neuraminidase (HN) molecule of Taka virus, were established. From the results of the cross haemagglutination-inhibition(HI) test the monoclonal antibodies, the HN molecule of Taka virus seemed to have at least three different antigenic determinats; one was specific for all NDV strain tested, the second was only for Taka virus and the third was for Take virus, Banger and Yucaipa Furthermore the differences in the ratio of HI to neuraminidase-inhibition titers suggested that the active sites involved in HA and neuraminidase activities might be different from each other. However, since each of five monoclonal anitbodies was not especially specific for either HA or neuraminidase, the possibility that a single active site on the HN molecule may be responsible for both activities has not been excluded.

• Asian-Australasian Journal of Animal Sciences
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• 제17권4호
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• pp.560-563
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• 2004

The study was undertaken to evaluate the effect of naked neck gene on mortality, cell mediated and humoral immune response in white plumage broiler population. The mortality of homozygous naked neck (Na/Na) broilers (11.71%) was comparatively lower than that of heterozygous naked neck (Na/na) (12.28%) and normally feathered (na/na) (13.59%) broilers. The humoral immune response was measured against (1% v/v) sheep red blood cells (SRBC) for total haemagglutinin (HA) antibody, 2-mercaptoethanol resistance (MER) or (IgG) antibody and 2-mercaptoethanol sensitive (MES) or (IgM) antibody titre on 7 days post-immunization. The titre was expressed as log2 of the highest dilution which shows complete haemagglutination. Total HA titers of Na/Na and Na/na (11.05$\pm$0.53 and 11.09$\pm$0.38) were comparatively higher than that of na/na (10.26$\pm$0.42). The MES antibody titre of Na/Na (8.50$\pm$0.53) and Na/na (7.63$\pm$0.45) broilers were significantly higher as compared to na/na (6.11$\pm$0.32) broilers. The MER titre of na/na genetic group (4.15$\pm$0.42) was significantly higher than Na/Na (2.55$\pm$0.37) and comparatively higher than Na/na (3.45$\pm$0.38) broilers. In vivo cell response to phytohaemagglutinin-P (PHA-P), measured as Foot Index (FI) in mm expressed significantly higher response in Na/na (0.473$\pm$0.05) and Na/Na (0.413$\pm$0.04) broilers as compared to na/na (0.304$\pm$0.03) broilers. The result of present study suggested that white plumage naked neck broilers had better immune response as compared to normally feathered broilers.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.135.3-136
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• 2003

In the present study, type A influenza live virus, NC-22-8, which is a combination of a cold-adapted attenuated donor virus (HTCA-A101) and a wild type virus (A/New Caledonia/20/99), was constructed and the efficacy of this new virus was assessed by immunogenicity and protection tests in the mouse model. NC-22-8 (1'$10^7, 1'10^5, 1'10^3$ pfu/mouse) was intranasally administered to mice. Four weeks later, the titers of specific IgG and haemagglutinin inhibiton (HI) were measured from blood and the titer of secretary IgA (sIgA) was also detected from boncho alveolar lavage (BAL) and mucosal fluid. (omitted)

• Toxicological Research
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• 제32권4호
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• pp.269-274
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• 2016

The potency of influenza vaccine is determined based on its hemagglutinin (HA) content. In general, single radial immunodiffusion (SRID) assay has been utilized as the standard method to measure HA content. However, preparation of reagents for SRID such as antigen and antibody takes approximately 2~3 months, which causes delays in the development of influenza vaccine. Therefore, quantification of HA content by other alternative methods is required. In this study, we measured HA contents of H1N1 antigen and H1N1 influenza vaccine by reverse phase-high performance liquid chromatography (RP-HPLC) methods. The presence of HA1 and HA2 was investigated by silver staining and Western blot assay. In addition, accuracy and repeatability of HA measurement by RP-HPLC were evaluated. Comparison of HA concentration by SRID and RP-HPLC revealed a precise correlation between the two methods. Our results suggest that RP-HPLC assay can replace SRID in the event of a pandemic flu outbreak for rapid vaccine development.

• 대한수의학회지
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• 제38권1호
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• pp.53-63
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• 1998

Total of 1085 swine sera (1996-1997) from nation-wide were tested for the presence of antibodies to influenza A virus. Fifty nine percent of the tested sera showed seropositive by HI test. Positive sera consisted of 24--- of H3, 15--- of H1, and 20--- of the sample had both antibodies, respectively. Sera collected from various region represented 7~27--- seropositivity to H1N1, 15~25--- to H3N2, respectively. Swine influenza field isolate from nasal swab was characterized antigenically and genetically to elucidate its relatedness with other known strains of influenza A virus. The study was focused on the HA gene which is related to pathogenecity and antigenic variability of the influenza virus. By RT-PCR using influenza A/H1N1 specific primers, influenza virus H1N1 specific DNA fragment was amplified from A/Swine/Iowa/15/30(H1N1), US field isolate but not in H3N2 strain. PCR products were sequenced by dideoxy chain termination method to determine nucleotide homology with other strains of influenza A virus. The US field isolate and A/Swine/Indiana/1726/88 strain had 97--- of nucleotide homology and 98--- of amino acid homology. Based on the results obtained from this experiment, the field isolate was genetically related to A/Swine/Indiana/1726/88 and had higher homology with A/Swine/Indiana/1726/88 than with classical swine influenza virus, A/Swine/Iowa/15/30. The field isolate had no amino acid changes at the antigenic site compare to that of the A/Swine/Indiana/1726/88. The proteolytic enzyme cleavage site between HA1 and HA2 had no alteration and the amino acid arginine was intact. There is no evidence has been found that the field isolate has genetic shift or genetic drift which might altered antigenic determinant.

• 대한수의학회지
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• 제12권1호
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• pp.77-84
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• 1972

이 실험(實驗)은 뇌염(腦炎)바이러스(MVE virus)들 acetone, Tween-ether 그리고 Tween-ether-protamine-sulphate 로 처리한 후 그 바이러스가 지니고 있는 적혈구응집력가(赤血球凝集力價), 보체결합력가(補體結合力價) 그리고 감염력가(感染力價)의 변화 여부를 관찰함과 아울러 초원심분리법(超遠心分離法)에 의하여 이 바이러스가 지니고 있는 위의 세 가지 활성물질(活性物質)의 분획(分劃) 가능성(可能性)을 검토한 결과 다음과 같은 결과를 얻었다. 1) Acetone, Tween-ether 그리고 Tween-ether protamine-sulphate 처리에 의하여 MVE virus가 지니는 적혈구응집력가(赤血球凝集力價)는 8~16 배(倍)로 증가 하였다. 2) Acetone 및 Tween-ether 처리로 보체결합력가(補體結合力價)는 4 배(倍)로 증가 되었으나 Tween-ether protamine-sulphate 처리로는 저하되는 경향이 있었다. 3) Tween-ether로 처리하면 감염력가(感染力價)는 완전히 상실 되나 acetone으로 처리하면 $TCID50/log_{10}$ 3이 감퇴 되었다. 4) 위의 화학제의 처리와 관계없이 바이러스의 적혈구응집력가(赤血球凝集力價)는 $37^{\circ}C$ 에서 10 분간 가열(加熱)됨으로써 완전 소실 되었으나 보체결합력가(補體結合力價)는 상승 하였으며 이 활성도(活性度)는 $65^{\circ}C$ 에서 20 분간 까지도 계속 유지되었다. 5) 처리되지 않은 바이러스와 acetone 처리된 바이러스의 보체결합력가(補體結合力價)나 적혈구응집력가(赤血球凝集力價)는 Tween-ether 나 Tween-ether-protamine-sulphate 로 처리한 바이러스의 그것보다 열(熱)에 대해서 더 안정(安定)하였다. 6) 적혈구응집억제반응용(赤血球凝集抑制反應用) 항원제조(抗原製造)에 있어서 Tween-ether 로 처리하거나 acetone 으로 처리하여 만든 두가지 항원(抗原)은 모두 동일(同一)한 역가(力價)를 보였다. 7) 10~60%의 sucrose gradient centrifugation 에서 처리되지 않은 바이러스는 두개의 보체결합(補體結合)피크가 저농도(低濃渡)와 고농도(高濃渡)에서 각각 나타났으며 저농도(低濃渡)에서 얻은 재료는 적혈구응집력(赤血球凝集力)과 감염력(感染力)을 동반하고 있었다. 8) Acetone 으로 처리된 바이러스의 초원심분리(超遠心分離)에서는 위에 말한 두 보체결합(補體結合)피크 중 고농도부(高濃渡部)의 것은 감소 되었으나 다른 하나는 영향을 받지 않았다. 그리고 적혈구응집력가(赤血球凝集力價)가 증가된 반면 감염최고력가(感染最高力價)는 감퇴 되었으며 이 세 가지 활성(活性)은 acetone 처리 후에는 명확히 분획(分劃)되지 않았다. Tween-ether 로 처리된 바이러스는 초원심분리(超遠心分離)에 의하여 고농도부(高濃渡部)에 있는 보체결합(補體結合)피크는 완전 소실된 반면 저농도부(低濃渡部)에 있는 보체결합최고력가(補體結合最高力價)는 영향을 받지 아니 하였으며 이 피크는 감염력(感染力)을 동반하지 않는 적혈구응집소(赤血球凝集素)와 분획(分劃)이 가능 하였다.