• 화훼연구
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• 제18권4호
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• pp.295-298
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• 2010

'Biz' 품종은 경남농업기술원 화훼연구소에서 백색 홑꽃인 우수계통 'SP03-117'을 모본, 백색 홑꽃인'Angaesoguk'을 부본으로 인공교배를 실시하여 2004에서 2008년에 걸쳐서 파종과 계통선발을 통해 육성하였다. 품종의 특성검정은 2006년부터 2008년까지 봄 촉성재배와 가을 억제재배 조건아래서 3회에 걸쳐 조사하였다. '비즈' 품종의 자연개화기는 10월 26일경이며, 일장조절에 의해 주년 재배가 가능하다. 초장이 87.7 cm이고 꽃 크기가 3.0 cm인 산티니계의 스프레이국화로 착화수는 15.6개이다. 화색은 백색이며, 개화형태는 실린더형이다. 봄의 단일상태에서 'Biz' 품종의 개화소요일수는 42일 정도로 개화가 빠르며, 가을 절화수명검정에서 24.1일로 아주 긴 편이다.

• 화훼연구
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• 제18권3호
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• pp.211-215
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• 2010

'Maigic' 품종은 경남농업기술원 화훼연구소에서 복색 홑꽃인 'Artist Pink'를 모본, 복색 홑꽃인 'SP0347'을 부본으로 인공교배를 실시하여 2003년에서 2007년에 걸쳐서 파종과 계통선발을 통해 육성하였다. 품종의 특성검정은 2005년부터 2007년까지 봄 촉성재배와 가을 억제재배 조건아래서 3회에 걸쳐 조사하였다. 'Maigic' 품종의 자연개화기는 10월26일경이며, 일장조절에 의해 주년재배가 가능하다. 꽃 크기가 6.1 cm인 중륜화로 꽃 하나의 꽃잎수가 31.6개정도이고 착화수는 14개 정도이다. 화색은 복색(백색/자주색)이며, 녹색화심을 가졌다. 봄의 단일상태에서 'Maigic' 품종의 개화소요일수는 43일 정도로 개화가 빠르며, 가을의 절화수명은 20.6일로 나타났다.

• 한국육종학회지
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• 제40권3호
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• pp.348-351
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• 2008

시설절화 장미 '레드미'은 경남농업기술원 화훼연구소에서 육성한 신품종으로 다수성인 적색 대륜계의 'Violina'을 모본으로 장기 활력과 병해충에 강한 특성을 가진 대형품종인 '샤샤'를 부본으로 하여 인공교배를 2000년도에 실시하였다. 화형과 화색 그리고 절화수량, 가시가적은 계통을 1차 선발을 하였고 촉성재배 후 2-3차 선발을 통하여 GR0035-11 등 10계통을 양성하였으며 가장 우수한 형질을 가진 GR0035 -11번 계통을 최종선발 하였다, 2002년부터 2004년 3년에 걸쳐 생육, 수량 및 특성검정을 양액재배 방법으로 실시하였으며 절화수량은 194본/$m^2$/년이며 절화수명은 10일로 대비종인 '샤샤' 품종 보다 수명이 길었다. 활력은 강하고 삽목번식이 용이하고 발근력이 강하며 꽃 직경이 8.6 cm의 중형 스텐다드형이다. 화형은 반검변고심형 겹꽃으로 정연하게 피고 꽃잎수는 34.4개 정도 이며 절화장은 93.6 cm로 대비종 보다 길다. 2006년 장미 육성계통 평가회 및 농작물 직무육성 품종 선정위원회를 거처 '레드미'로 명명하여 2007년에 식물육성자권리(2007-111)와 품종명을 국립종자원으로부터 취득 하였다.

• 화훼연구
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• 제16권4호
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• pp.229-233
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• 2008

'Moulin Rouge' 품종은 경남농업기술원 화훼연구소에서 복색 홑꽃인 'Delmont'를 모본, 분홍색 홑꽃인 'Anjela'을 부본으로 인공교배를 실시하여 2002년에서 2006년에 걸쳐서 파종과 계통선발을 통해 육성하였다. 2004년부터 2006년까지 3회에 걸쳐 봄 촉성 및 가을 억제재배 특성을 검정하였다. 'Moulin Rouge' 품종의 자연개화기는 10월24일이며, 일장조절에 의해 주년재배가 가능하다. 꽃 크기가 5.3 cm인 중륜으로 꽃 하나의 꽃잎 수는 22.9개 이였으며, 착화수는 12.2개 이였다. 화색은 복색(황색/오렌지색)이며, 녹색의 화심을 가졌다. 봄의 단일상태에서 'Moulin Rouge' 품종의 개화소요일수는 55일이며, 가을 절화수명은 21.7일로 나타났다.

• 화훼연구
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• 제16권3호
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• pp.200-204
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• 2008

'Serapina' 품종은 경남농업기술원 화훼연구소에서 적색 홑꽃인 'Relanse'를 모본, 백색 홑꽃인 'Yen'을 부본으로 인공교배를 실시하여 2002년에서 2006년에 걸쳐서 파종과 계통선발을 통해 육성하였다. 품종의 특성검정은 2004년부터 2006년까지 봄 촉성재배와 가을억제재배 조건아래서 3회에 걸쳐 조사하였다. 'Serapina'품종의 자연개화기는 10월23일이며, 일장조절에 의해 주년재배가 가능하다. 꽃 크기가 2.8cm인 소륜으로 꽃 하나의 꽃잎수가 43.4개로 많은 편이며, 착화수도 28.9개로 다화성이다. 화색은 황색이며 녹색화심을 가졌다. 봄의 단일상태에서 'Serapina'품종의 개회소요일수는 46일이며, 가을 절화수명검정에서 21.7일로 나타났다.

• 한국균학회지
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• 제47권3호
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• pp.219-232
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• 2019

Chemicals related to hydrogen peroxide ($H_2O_2$) and nitric oxide (NO) generations were exogenously applied to Fusarium oxysporum f. sp. fragariae (Fof) causing Fusarium wilt disease in strawberry plants, and regulations of in vitro conidial germination and mycelial growth of the fungus by the chemical treatments were evaluated. $H_2O_2$ drastically reduced the conidial germination of Fof in a dose-dependent manner, and treatment with 3-amino-1,2,4-triazole (3-AT) catalase inhibitor also led to dose-dependent inhibition of conidial germination but relatively moderately. Gradual decreases in mycelial growth of Fof were found by high concentrations of $H_2O_2$, whilst exogenous 3-AT slightly increased the mycelial growth. Increasing sodium nitroprusside (SNP) NO donor, $N^G$-nitro-l-arginine methyl ester (L-NAME) NO synthase (NOS)-inhibitor and tungstate nitrate reductase (NR) inhibitor led to dose-dependent reductions in conidial germination of Fof in quite different levels. SNP conversely increased the mycelial growth but increasing L-NAME moderately decreased the mycelial growth. Tungstate strongly enhanced mycelial growth. Differentially regulated in vitro mycelial growths of Fof were demonstrated by SNP, L-NAME and tungstate with or without $H_2O_2$ supplement. Superoxide anion production was also regulated during the mycelial growth of Fof by nitric oxide. These results show that $H_2O_2$ and NO-associated enzymes can be suggested as fungal growth regulators of Fof as well as eco-friendly disease-managing agents in strawberry production fields.

• 식물병연구
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• 제24권4호
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• pp.292-301
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• 2018

Alternaria leaf spot disease has been a concern during a tomato production in greenhouse. In vitro antifungal activities of vapours of four plant essential oils, cinnamon oil, fennel oil, origanum oil and thyme oil, were investigated during in vitro conidial germination and mycelial growth of Alternaria alternata causing the tomato leaf spots to find eco-friendly alternatives for chemical fungicides. The four plant essential oils showed different antifungal activities against in vitro conidial germination of A. alternata in dose-dependent manners, and cinnamon oil vapour was most effective to suppress the conidial germination. The four plant essential oils showed similar antifungal activities against the in vitro mycelial growth of A. alternata in dose-dependent manners, but low doses of thyme oil vapour slightly increased in vitro mycelial growth of A. alternata. Necrotic lesions on the A. alternata-inoculated tomato leaves were reduced differently depending on kinds and concentrations of plant essential oils. Delayed conidial germination and germ-tube elongation of A. alternata were found on the tomato leaves treated with cinnamon oil and origanum oil vapours at 6 hpi. These results suggest that volatiles from cinnamon oil and origanum oil can be provided as alternatives to manage Alternaria leaf spot during the tomato production eco-friendly.

• Biomolecules & Therapeutics
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• 제30권4호
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• pp.360-367
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• 2022

Tropomyosin receptor kinase A (TrkA) protein is a receptor tyrosine kinase encoded by the NTRK1 gene. TrkA signaling mediates the proliferation, differentiation, and survival of neurons and other cells following stimulation by its ligand, the nerve growth factor. Chromosomal rearrangements of the NTRK1 gene result in the generation of TrkA fusion protein, which is known to cause deregulation of TrkA signaling. Targeting TrkA activity represents a promising strategy for the treatment of cancers that harbor the TrkA fusion protein. In this study, we evaluated the TrkA-inhibitory activity of the benzoxazole compound KRC-108. KRC-108 inhibited TrkA activity in an in vitro kinase assay, and suppressed the growth of KM12C colon cancer cells harboring an NTRK1 gene fusion. KRC-108 treatment induced cell cycle arrest, apoptotic cell death, and autophagy. KRC-108 suppressed the phosphorylation of downstream signaling molecules of TrkA, including Akt, phospholipase Cγ, and ERK1/2. Furthermore, KRC-108 exhibited antitumor activity in vivo in a KM12C cell xenograft model. These results indicate that KRC-108 may be a promising therapeutic agent for Trk fusion-positive cancers.

• 한국동물위생학회지
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• 제45권3호
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• pp.211-219
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• 2022

Bovine viral diarrhea (BVD) is one of the problematic wasting diseases in cattle leading to huge economic losses. This study was conducted to investigate the prevalence of BVD including transient and persistent infection from cattle farms in Gyeongsangnam-do. A total of 2,667 blood samples from 24 farms were collected and the sera were subjected to ELISA to detect BVD virus (BVDV) antigen, E^rns. 5' untranslated region (5'-UTR) of BVDV-positive samples was sequenced to identify the genotype, and compared with isolates previously reported elsewhere. There were fourteen BVDV-positive calves from 2,667 samples (positive rate: 0.52%) from first ELISA testing followed by eight persistently infected out of eleven BVDV-positive samples (72.73%) in secondary ELISA that was conducted in at least four weeks suggesting the circulation of BVDV in the area. Sequencing analysis exhibited that thirteen BVDV-positive samples were identified as BVDV-1b and one sample was BVDV-2a. Phylogenetic analysis revealed that the BVDV-1b-positive samples showed the highest homology in nucleotide sequence to Korean isolates collected from Sancheong, Gyeongsangnam-do, while the BVDV-2a-positive sample (21GN7) was more similar to reference strains collected outside South Korea. This study will provide the recent fundamental data on BVD prevalence in Gyeongsangnam-do to be referred in developing strategies to prevent BVDV in South Korea.

• 대한수의학회지
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• 제44권2호
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• pp.241-249
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• 2004

As a part of epidemiologic investigation of tsutsugamushi disease, the wild rodents which were captured in Gyeongnam area were diagnosed with indirect immunofluorescent antibody assay (IFA) and Polymerase Chain Reaction (PCR) to find if they have an antibody against Orientia tsutsugamushi. The conclusion was drawn as followings. (1) The captured 58 wild rodents showed that the subspecies distribution of Apodemus agrarius was 86.2%, Microtus fortis was 8.6% and Crocidura lasiura was 5.2%. (2) The antibody positive rate of O. tsutsugamushi Gilliam, Karp, Karto and Boryong by IFA method was 32.0% in Apodemus agrarius among 50 wild rodents and 40.0% in Microtus fortis among 5 wild rodents, respectively. It was negative in the case of all the 3 Crocidura lasiura. (3) The antibody titers on Apodemus agrarius, Microtus fortis and Crocidura lasiura against Gilliam, Karp, Karto and Boryong were measured between 1:20 and 1:640. The antibody titer against each antigen was in the order Boryong>Gilliam>Karp. (4) O. tsutsugamushi was detected from the blood, spleen and kidney from the artificially infected mice by IFA and PCR. IFA showed the positive response between 3 and 18 days after inoculation. On the other hand, positive response was found from all the samples by PCR. (5) From PCR of the genomic DNA extracted from the blood, spleen and kidney samples of the captured wild rodents, Boryong-specific amplification product with size of 210 bp, which is particular in Boryong, was detected from spleen and kidney samples, but not detected in the blood. (6) Boryong-specific amplification product was detected from spleen and kidney samples which were obtained at 3, 6, 12, 18 and 24 days after the infection with Boryong. But, it wasn't detected from the uninfected samples. (7) From PCR of spleen and kidney samples of the captured wild rodents, it was found that positive rate of O. tsutsugamushi in Apodemus agrarius and Microtus fortis were 25.0% (4/16) and 20.0% (1/5), respectively. From the above results, it can be concluded that Apodemus agrarius resided in Gyeongnam area carried O. tsutsugamushi and PCR method might be a simple, precise, rapid and useful diagnostic tool than IFA for the diagnosis of O. tsutsugamushi.