• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.144-144
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• 2017

Micronutrients such as zinc (Zn), iron (Fe), manganese (Mn) have important roles for development and growth in plants but it also have roles in animals and humans. In previous studies, a Korean weedy rice, KH2J was selected to have tolerance to heavy metal, lead (Pb) compared with a cultivar, Milyang23. To identify QTLs for micronutrients concentration in grain, an F2 population (120 plants) were developed from a cross between KH2J and an indica rice cultivar, Milyang23. To measure the concentration of eight ions, Zn, Fe, Mn, Pb, calcium (Ca), copper (Cu), cadmium (Cd) and arsenic (As), grains were collected and digested with 65% nitric acid, and the ion contents were measured using inductively coupled plasma mass spectrometry. A total 27 putative quantitative trait loci (QTLs) were detected on 12 chromosomes by single point analysis and 22 putative QTLs were detected by composite interval mapping. The co-locations of QTL for Zn, Fe and Mn were observed on chromosome 5. The QTLs for Cd, Cu and Zn were co-localized on chromosome 10, and QTLs for Zn, As and Mn was on chromosome 12. The Zn concentration in F2 generation showed significant correlation with concentrations of As (r = -0.4), Cu (r = 0.5) and Fe (r = 0.2) (P < 0.01). Also, the Ca concentration was significantly related with Mn and Fe concentrations (P < 0.01). Fine mapping of these QTLs is underway to analyze their functional relationship.

• The Korean Journal of Malacology
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• v.24 no.2
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• pp.121-130
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• 2008

In other aquaculture species, large improvements in growth have been achieved through selective breeding. Ezo abalone(Haliotis discus hannai) and disk abalone(H. discus discus) are major aquatic animals cultured in Asia, but selective breeding for the promotion of growth with these abalones has not been actively pursued. Recently significant efforts are being made to promote production of these species through selective breeding in Korea. The aims of this work were to estimate the general genetic parameters, heritabilities, and genetic and phenotypic correlations on growth-related traits at 1-year old in two Korean abalone subspecies, H. discus hannai and H. discus discus, by using multiple trait animal model. The data were collected from the records of 1,504 individuals produced from 22 sires and 26 dams in H. discus hannai and 297 individuals produced from 5 sires and 6 dams in H. discus discus, which evaluated by the Genetics and Breeding Research Center, National Fisheries Research & Development Institute(NFRDI). Genetic parameters were estimated for these abalone subspecies raised in Bukjeju branch, NFRDI, from May 20, 2004 to May 16, 2005, respectively. The heritability estimates obtained from restricted maximum likelihood(REML) were higher than expected, ranging from 0.40 to 0.43 for growth traits shell length, shell width and body weight in H. discus hannai and from 0.26 to 0.51 in H. discus discus, respectively. The heritabilities for shell shape and condition factor were lower than others of growth traits such as ranging from 0.09 to 0.19 in H. discus hannai and from 0.10 to 0.23 in H. discus discus, respectively. Genetic and phenotypic were > 0.93 between shell parameters and weight in two abalone species, respectively, indicating that breeding for weight gains could be successfully achieved by selecting for shell length.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.312-325
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• 2015

In this review, we summarized the trends of genomics and transcriptomics research on peach, a model species of Rosaceae. Peach genome maps have been developed from various progeny groups with many next-generation sequencing (NGS) based single nucleotide polymorphism markers. Molecular markers of qualitative traits and quantitative trait loci (QTL) such as fruit characteristics, blooming date, and disease resistance have been analyzed. Among many characteristics, markers related to flesh softening and flesh adhesion are useful for marker assisted selection. Through comparative genomics, peach genome has been compared to the genome of Arabidopsis, Populus, Malus, and Fragaria species. Through transcriptomics and proteomics, fruit growth and development, and flavonoid synthesis, postharvest related transcriptomes and disease resistance related proteins have been reported. Recently, development of NGS based markers, construction of core collection of germplasm, and genotyping of various progenies have been preceded. In the near future, accurate QTL analysis and identification of useful genes are expected to establish a foundation for effective molecular breeding.

• (The) Research of the performance art and culture
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• no.36
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• pp.531-551
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• 2018

The educational trait that the arts and cultural education and dance strive for at a time when the ethical tasks of life is the experience for insight of life. The awareness of time entrusted with the intensity [depth] of artistic and aesthetic experience is to contain its implication with policy and system. In the policy territory, broad perception and strategy are combined and practiced to produce new implication. Therefore, on the basis of characteristics and spectrum persuaded at a time when the arts and cultural education and dance education are broadly expanded, the result of this study after taking a look at the role of dance education within the arts and cultural education is shown as follows. The value striving for by the culture and arts education and dance education is to structure the life form with the artistic experience through the art as the ultimate life description. This is attributable to the fact that the artistic trait structured with self-understanding and self-expression contains the directivity of life that is recorded and depicted in the process of life. The dance education in the culture and arts education has the trait to view the world with the dance structure as the comprehensive study as in other textbook or art genre under the awareness of time and education system category within the school system and it has diverse social issues combined as related to the frame of social growth and advancement outside of school. When taking a look at the practical characteristics (method) of dance based on the arts and cultural education business, it facilitates the practice strategy through dance, in dance, about dance, between dance with the artist for art [dance]. At this time, the approachability of dance is deployed in a program based on diverse artistry for technology, expression, understanding, symbolism and others and it has the participation of enjoyment and preference. In the policy project of the culture and arts education, the dance education works as the function of education project as an alternative model on the education system and it also sometimes works as the function for social improvement and development to promote the community awareness and cultural transformation through the involvement and intervention of social issues.

• The Korean Journal of Malacology
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• v.23 no.2
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• pp.217-225
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• 2007

This study was conducted to estimate the general genetic parameters, heritabilities, and genetic and phenotypic correlations on growth-related traits by studying multiple trait animal model in two Korean abalone species, Haliotis discus hannai and H. discus discus. The data was collected from the records of 3,795 individuals produced from 54 sires and 74 dams in Haliotis discus hannai and 399 individuals produced from 7 sires and 7 dams in Haliotis discus discus. The data was evaluated by the Genetics and Breeding Research Center, National Fisheries Research & Development Institute (NFRDI). Genetic parameters were estimated for two abalone species raised in Bukjeju branch, NFRDI, from May 20 to November 1, 2004. The heritability estimates for growth traits of shell length, shell width and body weight obtained from restricted maximum likelihood (REML) were ranging from 0.73 to 0.78 in Haliotis discus hannai, and from 0.87 to 0.89 in H. discus discus. The heritabilities for shell shape and condition factor were ranging from 0.17 to 0.20 in Haliotis discus hannai, and from 0.01 to 0.45 in H. discus discus. Genetic and phenotypic correlations were over than 0.96 between shell parameters and weight in both of abalone subspecies, indicating that breeding for weight gains could successfully be achieved by selecting for shell length.

• Korean Journal of Breeding Science
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• v.43 no.1
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• pp.68-80
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• 2011

This study was conducted to investigate characteristics and variations of rice panicle traits for breeding temperate japonica varieties adapted to tropical environment. Eleven panicle traits were investigated from nine Korean rice varieties cultivated in Korea and wet season of the Philippines. Tested cultivars were composed of six temperate japonica varieties, three Tongil-type varieties, and one indica variety bred in the Philippines. The number of spikelets on secondary rachis branches (SRBs) was the most variable trait in both environments, while the mean number of spikelets on a primary rachis branch (PRB) was the least variable. Compared with PRB-related traits, SRB traits showed higher correlation with the number of spikelets per panicle. Compared with the plants grown in Korea, the number of spikelets on SRBs, the number of SRBs, spikelets, and rachis branches per panicle were decreased more than other traits in the Philippines. According to path analysis, the number of spikelets on SRBs per panicle affects the number of spikelets per panicle more than the number of spikelets on PRBs per panicle. Climatic factors such as growth duration, cumulative mean temperature, and integrated solar radiation were highly correlated with the relative rate of number of spikelets per panicle. To breed temperate japonica rice varieties adapted to tropical environment, it would be important to select lines which maintain proper growth duration and spikelets on SRBs in target region.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.1
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• pp.85-92
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• 2008

An RIL population from a Shinpaldalkong2/GC83006 cross was employed to identify quantitative trait loci (QTL) associated with agronomic traits in soybean. The genetic map consisted of 127 loci which covered about 3,000cM and were assigned into 20 linkage groups. Phenotypic data were collected for the following traits; plant height, leaf area, flowering time, pubescence color, seed coat color and hilum color in 2005. Seed weight was evaluated using seeds collected in 2003 to 2005 at Suwon and in 2005 at Pyeongchang and Miryang sites. Three QTLs were associated with 100-seed weight in the combined analysis across three years. Among the three QTLs related to seed weight, all GC83006 alleles on LG O ($R^2\;=\;12.5$), LG A1 ($R^2\;=\;10.1$) and LG C2 ($R^2\;=\;11.5$) increased the seed weight. A QTL conditioning plant height was linked to markers including Satt134 (LG C2, $R^2\;=\;25.4$), and the GC83006 allele increased plant height at this QTL locus. For two QTLs related to leaf area, 1aM on LG M ($R^2\;=\;10.0$) and laL on LG L ($R^2\;=\;8.6$), the Shinpaldalkong2 alleles had positive effect to increase the leaf area. Satt134 on LG C2 ($R^2\;=\;41.0$) was associated with QTL for days to flowering. Satt134 (LG C2) showed a linkage to a gene for pubescence color. Satt363 (LG C2) and Satt354 (LG I) were linked to the hilum color gene, and Sat077 (LG D1a) was linked to the seed coat color. The QTL conditioning plant height was in the similar genomic location as the QTLs for days to flowering in this population, indicating pleiotropic effect of one gene or the tight linkage of several genes. These linked markers would be useful in marker assisted selection for these traits in a soybean breeding program.

• Journal of Korean Society of Forest Science
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• v.112 no.1
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• pp.40-56
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• 2023

Tree species within the Populus genus grow rapidly and have an excellent capacity to absorb carbon, conferring substantial ability to effective purify the environment. Poplar breeding can be achieved rapidly and efficiently if a genetic linkage map is constructed and quantitative trait loci (QTLs) are identified. Here, a high-density genetic linkage map was constructed for the control pollinated progeny using the genotyping-by-sequencing (GBS) technique, which is a next-generation sequencing method. A search was also performed for the genes associated with quantitative traits located in the genetic linkage map by examining the variables of height and diameter at root collar, and resilience to insect damage. The height and diameter at root collar were measured directly, while the ability to recover from insect damage was scored in a 4-year-old breeding population of aspen hybrids (Odae19 × Bonghyeon4 F1) established in the research forest of Seoul National University. After DNA extraction, paternity was confirmed using five microsatellite markers, and only the individuals for which paternity was confirmed were used for the analysis. The DNA was cut using restriction enzymes and the obtained DNA fragments were prepared using a GBS library and sequenced. The analyzed results were sorted using Populus trichocarpa as a reference genome. Overall, 58,040 aligned single-nucleotide polymorphism (SNP) markers were identified, 17,755 of which were used for mapping genetic linkages. The genetic linkage map was divided into 19 linkage groups, with a total length of 2,129.54 cM. The analysis failed to identify any growth-related QTLs, but a gene assumed to be related to recovery from insect damage was identified on linkage group (chromosome) 4 through genome-wide association study.

• Korean Journal of Ichthyology
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• v.26 no.3
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• pp.185-193
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• 2014

To find the influence of water temperature, tank color and illumination in feeding, growth and blind-side hypermelanosis of starry flounder, Platichthys stellatus, in the present study, we performed a series of temperature, background color and illumination intensity test for 180 days (From June to December). The test was done in duplicate at 100 fish/tank (430% of initial covering area [PCA]) with the selected ordinary juvenile flounder (TL $17.3{\pm}0.5cm$, BW $82.5{\pm}0.2g$). The rearing was performed in darkgreen FRP aquarium tanks ($H100cm{\times}L100cm{\times}W100cm$; bottom area $1m^2$) lighten with average 1,000 lux and 230 lux, and in white FRP aquarium tank ($H100cm{\times}L100cm{\times}W100cm$; bottom area $1m^2$) lighten with average 230 lux of light intensity. We investigated correlation of daily food intake (DFI) with water temperature and salinity, and compared the influences of background colors and light intensity in DFI, food efficiency (FE), growth, survival rate, and ratio of malpigmented blind-side area and ambicolored fish ratio. In DFI, although it was not related with salinity, the amount was significantly decreased under 0.5 g/fish/day in summer and winter season, but was significantly increased over 1.5 g/fish/day in autumn season showing from $10^{\circ}C$ to $20^{\circ}C$ in water temperature. In background and illumination test, DFI, FE and survival rate showed no difference among three groups. The ratios of malpigmented blind-side area and ambicolored fish were also not significantly different among three groups, indicating that the blind-side hypermelanosis of starry may be governed not by background color (or light intensity) but by a genetics external trait inherited from parents.

• The Korean Journal of Malacology
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• v.29 no.4
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• pp.325-334
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• 2013

The purpose of this paper is to compare and analyze family variations for growth-related traits of Pacific abalone, Haliotis discus hannai. Genetic parameters and breeding values were estimated using all measurement data like shell length, shell width, and total weight as 18-month-old growth traits of 5,334 individuals of selected third generation's Pacific abalone produced in 2011. Family variations of 865 individuals of the upper 10 families with the largest number were inspected. Overall mean in phenotypic traits of 18-month-old Pacific abalone which was investigated in this study showed 54.5 mm of shell length, 36.8 mm of shell width and 21.3 g of total weight respectively. And, variation coefficient of total weight was 51.0%, so variability of data was shown to be higher than 21.1% of shell length and 20.7% of shell width. The family effects showed significant difference by each family (p < 0.05), and heritability of shell length, shell width, and total weight was medium with 0.370, 0.382, and 0.367 respectively. So it is considered that family selection is more advantageous than individual selection. On the basis of breeding values of estimated shell length and total weight, to investigate distribution and ranking by each individual about the upper 10 families with the largest number of individuals, the values were used by being changed into standardized breeding values. Based on shell length, it was investigated that the individual number of the upper 5.4% is 152 and the number of the lower 5.4% is 8. In case of total weight, it was inspected that the individual number of the upper 5.4% is 164 and the number of the lower 5.4% is 1. Like these, phenotypic and genetic diverse variations between families could be checked. By estimating genetic parameters and breeding values of a population for production of the next generation, if they are used properly in selection and mating, it is considered that more breeding effects can be expected.