• Journal of Plant Biotechnology
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• v.34 no.4
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• pp.285-291
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• 2007

This study describes conditions for the mass production of activation-tagged mutant hairy root lines of ginseng by cocultivation with Agrobacterium rhizogenes. Because it is not currently possible to produce progeny from transgenic ginseng, a loss-of-function approach for functional genomics cannot be appliable to this species. A gain-of-function approach is alternatively the choice and hairy root production by cocultivation of A. rhizogenes would be most practical to obtain a large number of mutants. Various sources of explants were subjected to genetic transformation with various strains of A. rhizogenes harboring the activation-tagging vector pKH01 to determine optimum conditions for the highest frequency of hairy root formation on explants. Petiole explants cocultivated with A. rhizogenes R1000 produced hairy roots at a frequency of 85.9% after 4 weeks of culture. Conditions for maximum growth or branching rate of hairy roots were also investigated by using various culture media. Petiole explants cultured on half strength Schenk and Hildebrandt medium produced vigorously growing branched roots at a rate of 2.6 after 4 weeks of culture. A total of 1,989 lines of hairy root mutants were established in this study. These hairy root lines will be useful to determine functions of genes for biosynthesis of ginsenosides.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.36 no.4
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• pp.421-428
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• 2012

In this study, the effects of surface oxide film formation on the fatigue behavior of 7075-T6 aluminum alloy were analyzed in terms of the corrosion time of the alloy. The aluminum material used is known to have high corrosion resistance due to the passivation phenomenon that prevents corrosion. Aluminum alloys have been widely used in various industrial applications such as aircraft component manufacturing because of their lighter weight and higher strength than other materials. Therefore, studies on the fatigue behavior of materials and passivation properties that prevent corrosion are required. The fatigue behavior in terms of the corrosion time was analyzed by using a four-pointing bending machine, and the surface corrosion level of the aluminum material in terms of the corrosion time was estimated by measuring the surface roughness. In addition, fractographic analysis was performed and the oxide films formed on the material surface were studied by scanning electron microscopy (SEM). The results indicated that corrosion actively progressed for four weeks during the initial corrosion phase, the fatigue life significantly decreased, and the surface roughness increased. However, after four weeks, the corrosion reaction tended to slow down due to the passivation phenomenon of the material. Therefore, on the basis of SEM analysis results, it was concluded that the growth of the surface oxide film was reduced after four weeks and then the oxide film on the material surface served as a protection layer and prevented further corrosion.

• Korean Journal of Medicinal Crop Science
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• v.5 no.1
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• pp.21-27
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• 1997

This experiments were conducted to determine the effect of thidiazuron on forming tuberlets and plant regeneration of Pinellia ternata T. by tissue culture. The addition of $5\;{\mu}M$ TDZ to the medium had better regeneration than that of any other treatments of NAA and TDZ. At the combination treatments of NAA and TDZ, as the level of thidiazuron increased, the rate of shoot regeneration was incresed while the increment of NAA concentration inhibited the rate of shoot regeneration. The supplement of $5\;{\mu}M$ thidiazuron produced the best number of micro-tubers per explant and the number of micro-tuber formed was 25 in MS medium and 29 in MG medium on 30 day culture, respectively. Microtuber formation was the best on MG medium with 1.0 mg/l NAA and $5\;{\mu}M$ thidiazuron. MG medium was superior to MS and B5 medium for the growth of tuberlets. Half strength of MS medium with NAA 2 mg/l was the most effective for root formation. Rooting ability on nursery soil of plantlets produced in in vjtro was good as a 80% after 3 weeks.

• Korean Journal of Materials Research
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• v.10 no.6
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• pp.403-408
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• 2000

The Al-Cr-Zr composite metal powders were prepared by mechanical alloying and consolidated by vacuum hot pressing. The microstructural characteristics and the thermal stability of the MA Al-Cr-Zr alloys were evaluated by means of microhardness measurement, XRD and TEM in order to develop high temperature, high strength aluminum alloys. The mechanical alloying was conducted in attritor with 300rpm for 20 hours. The density of the vacuum hot pressed Al-Cr-Zr alloy reached at 97% of theoretical one. After exposing at $300^{\circ}C$ for 100 hours, there is almost no variation in hardness change of the MA alloys. Even after exposing at $ 500^{\circ}C$ for 100 hours, the hardness of the alloy was decreased within 6% of the initial value. The fine stable $Al_3Zr\;and\; Al_{13}Cr_2$ intermetallics were formed at the stage of consolidation and heat treatment in aluminum matrix. The good thermal stability of the MA Al-Cr-Zr alloy can ab attributed to the role of the dispersoids, inhibiting grain growth of nanocrystalline, and the final grain size after heat treatment was less than 150nm.

• Analytical Science and Technology
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• v.30 no.1
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• pp.10-19
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• 2017

Anabolic steroids have similar structures to testosterone, both of which promote the growth of muscle mass and increase strength. However, the side effects of anabolic steroid use may lead to heart attacks or strokes. Additionally, the excessive use of steroids inhibits the production of the sex hormones in the body via a negative feedback loop, which results in testicular atrophy in males and amenorrhea in females. Currently, the method of choice used to test for the presence of anabolic steroids is GC-MS. However, GC-MS methods require chemical derivatization of the steroid sample to ensure compatibility with the analytical method; therefore, analysis of many different samples is difficult and time consuming. Unlike GC-MS, the liquid chromatography-quadrupole-time of flight mass spectrometry (LC-Q-TOF-MS) method is suitable for many samples. Twenty-two different anabolic steroids were analyzed by LC-Q-TOF-MS with various collision energies (CE). Accurate mass spectral data were obtained using a Q-TOF-MS equipped with an electro-spray ionization source and operated in the positive MS/MS mode for several classes of steroids that are often the targets of testing. Based on the collected data, fragmentation pathways were carefully elucidated. The high selectivity and sensitivity of the LC-Q-TOF-MS instrument combined with these fragmentation pathways offers a new approach for the rapid and accurate screening of anabolic steroids. The obtained data from the 22 different anabolic steroids will be shared with the scientific community in order to establish a library to aid in the screening of illegal anabolic steroids.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.4
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• pp.420-428
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• 1988

Root is supposed to be important role in growth and yield formation of soybeans and also there is expected to exist varietal differences between soybean root morphology and system. 30 soybean varieties including 10 Korean recommended, 10 Introduced and 10 Korean native varieties were grown at field and pot under standard culture condition. Top and root characteristics were investigated at V4, R1 and R5 stage and T/R relations, correlations with yield were calculated. Top characteristics showed significant difference between 3 variety groups of Korean recommended, Introduced and Korean native variety. Stem length at R1 stage and stem length, stem diameter, number of nodes, number of branches, number of pods and grain yield at R5 stage were significantly different top characteristics. Root characteristics showed significant difference between 3 variety groups were number of thick primary root at V4 stage, primary root angle and root cutting strength at R1 stage, thick root diameter and root dry weight at R5 stage. The correlations between top and root characteristics and soybean yield was greatly different by soybean variety groups, and the top characteristics was more highly correlated then root characteristics. All the top and root characteristics of Korean recommended variety showed no correlations with yield. However, leaf area, stem length, top dry weight in Introduced variety and leaf area, top dry weight, number of nodes, number of thick primary root in Korean native variety were highly correlated with soybean yield.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.6
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• pp.550-556
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• 1992

Performance of a rice breeding line, Milyang 95 was evaluated at four cultural methods, direct seeding on dry soil covered by making ridges (DS ridged), direct seeding on dry soil covered by rotortilling (DS rotary), direct seeding on flooded soil (FS), and machine transplanting (MT). Days from seeding to emergence in both DS ridged and DS rotary was 15 days. The number of seedlings at DS ridged and DS rotary was lower than that at FS. Heading was earliest at MT, latest at DS rotary and DS ridged, and that at FS was between them. Days from seeding to heading was 115 days at MT, 94-95 days at DS ridged and DS rotary, and 87 days at FS. Lodging index was similar among the cultural methods and lodging was not occurred in the field although fresh weight of tillers and breaking strength at MT were higher than those of direct seedings. Yield and most of yield components were similar among the cultural methods although the number of spikelets per panicle at MT was higher and 1,000 grain weight at FS was lower compared to other cultural methods. Grain appearance (rusty, chalky abortive rice), protein and amylose contents and alkali digestibility were observed.

• Journal of the Korea institute for structural maintenance and inspection
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• v.21 no.6
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• pp.67-73
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• 2017

Recently, the demand for large diameter piles has been rapidly increased in order to secure the allowable bearing capacity of pile foundation due to the increase of large structures such as high rise buildings. In this study, to improve the shear capacity of a conventional PHC pile, a large diameter composite PHC pile strengthened by in-filled concrete and shear reinforcement was manufactured. All the piles were tested according to the shear strength test method of Korean Standard. As a result of the shear test, the F-type piles which are produced without shear reinforcement occurred abrupt horizontal cracks after flexural and inclined shear cracks occurred. On the contrary, the FT-type piles which are produced with shear reinforcement exhibited stable flexural and inclined shear cracks uniformly over the entire pile without abrupt horizontal cracks. Furthermore, the maximum load of the large diameter composite PHC pile improved to 2.9 times in the F series, and more than 3.3 times in the FT series compared to the conventional PHC pile. This result indicated that FT-type piles had excellent composite behavior due to the shear reinforcement and effectively prevented the unstable growth of inclined shear cracks.

• Journal of the Economic Geographical Society of Korea
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• v.18 no.4
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• pp.415-430
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• 2015

Since the late 1990s Vinacafe has gone through strategic changes in corporate governance and managements due to an increase in the introduction of coffee MNCs, a growth of global demands in sustainable coffee, aging coffee tree, and the deterioration of coffee production with climate changes in Vietnam. Vinacafe has attempted to cope with these kinds of changes through strategies for equitization. Therefore, the main aim of this paper is to identify strategies for enhancing the competitiveness of the Vietnamese coffee industry by investigating changes in corporate governance and processes of coffee production and distribution. The equitization of Vinacafe has led to the enhancement of coffee competitiveness in two perspectives. Firstly, as it has decentralized decision-making from headquarter, subsidiaries have become able to strength their competitiveness themselves by introducing new technologies, improving coffee quality, and encouraging the introduction of eco-friendly production methods through cooperative relationships with stakeholders involved in coffee production and distributions in Vietnam. Secondly, it has also enhanced competitiveness through the diversification and effectiveness of coffee managements by intensifying the flexibility of contract with coffee farmers and diversifying coffee sales and supply chains in Vietnam.

• Journal of Korea Port Economic Association
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• v.32 no.4
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• pp.193-209
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• 2016

The Mekong River is a river in the south-eastern part of the continent of Aisa. It flows through the countries of Thailand, Lao PDR, Cambodia, Myanmar, and Vietnam that are located in Indochina and are members of ASEAN. These countries are growing rapidly and many others have entered into these markets. As the number of manufactures has increased, logistics markets become very attractive to our logistics companies that want to expand their business. This study focuses on four countries, Lao PDR, Cambodia, Myanmar, and Vietnam, which have potential economic growth and shows the environment of logistics with current logistics infrastructure and related investment law and system. The goal of this study to provide, with strength, weakness, opportunities, and threats(SWOT) analysis, some strategies to enter 4 countries' logistics market with SWOT and the strategies are as follows; First, foreign direct investment in logistics is linked with logistics infrastructure projects. Our government should strengthen its role to find cooperation programs that make connect with logistics business. Second, a logistics company is better off in a consortium with other manufacturers or other logistics companies to ensure minimum cargo and reduce entry risks. Finally, the four countries' roles as a logistics bases need to divided according to their environments, to benefits of logistics connecting between India and China.