• Journal of Microbiology and Biotechnology
• /
• v.31 no.10
• /
• pp.1343-1349
• /
• 2021

Cockroaches live in places where various pathogens exist and thus are more likely to use antimicrobial compounds to defend against pathogen intrusions. We previously performed an in silico analysis of the Periplaneta americana transcriptome and detected periplanetasin-5 using an in silico antimicrobial peptide prediction method. In this study, we investigated whether periplanetasin-5 has anticancer activity against the human leukemia cell line K562. Cell growth and survival of K562 cells treated with periplanetasin-5 were decreased in a dose-dependent manner. By using flow cytometric analysis, acridine orange/ethidium bromide (AO/EB) staining and DNA fragmentation, we found that periplanetasin-5 induced apoptotic and necrotic cell death in leukemia cells. In addition, these events were associated with increased levels of the pro-apoptotic proteins Fas and cytochrome c and reduced levels of the anti-apoptotic protein Bcl-2. Periplanetasin-5 induces the cleavage of pro-caspase-9, pro-caspase-8, pro-caspase-3, and poly (ADP-ribose) polymerase (PARP). The above data suggest that periplanetasin-5 induces apoptosis via both the intrinsic and extrinsic pathways. Moreover, caspase-related apoptosis was further confirmed by using the caspase inhibitor carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone (Z-VAD-FMK), which reversed the periplanetasin-5-induced reduction in cell viability. In conclusion, periplanetasin-5 caused apoptosis in leukemia cells, suggesting its potential utility as an anticancer therapeutic agent.

• Korean Journal of Remote Sensing
• /
• v.37 no.6_2
• /
• pp.1891-1900
• /
• 2021

Fine particulate matter (FPM; diameter ≤ 2.5 ㎛) is frequently found in metropolitan areas due to activities associated with rapid urbanization and population growth. Many adolescents spend a substantial amount of time at school where, for various reasons, FPM generated outdoors may flow into indoor areas. The aims of this study were to estimate FPM concentrations and categorize types of FPM in schools. Meteorological and chemical variables as well as satellite-based aerosol optical depth were analyzed as input data in a random forest model, which applied 10-fold cross validation and a grid-search method, to estimate school FPM concentrations, with four statistical indicators used to evaluate accuracy. Loose and strict standards were established to categorize types of FPM in schools. Under the former classification scheme, FPM in most schools was classified as type 2 or 3, whereas under strict standards, school FPM was mostly classified as type 3 or 4.

• Journal of Plant Biotechnology
• /
• v.48 no.3
• /
• pp.148-155
• /
• 2021

Gibberellins (GAs) are essential phytohormones for plant growth that influence developmental processes and crop yields. Recent functional genomic analyses of model plants have yielded good characterizations of the canonical GA signaling pathways and related genes. Although Panax ginseng has long been considered to have economic and medicinal importance, functional genomic studies of the GA signaling pathways in this crucial perennial herb plant have been rarely conducted. Here, we identified and performed functional analysis of the GA signaling-related genes, including PgGID1s, PgSLY1s, and PgRGAs. We confirmed that the physiological role of GA signaling components in P. ginseng was evolutionarily conserved. In addition, the important functional domains and amino acid residues for protein interactions among active GA, GID1, SCF^SLY1, and RGA were also functionally conserved. Prediction and comparison of crystallographic structural similarities between PgGID1s and AtGID1a supported their function as GA receptors. Moreover, the subcellular localization and GA-dependent promotion of DELLA degradation in P. ginseng was similar to the canonical GA signaling pathways in other plants. Finally, we found that overexpression of PgRGA2 and PgSLY1-1 was sufficient to complement the GA-related phenotypes of atgid1a/c double- and rga quintuple-mutants, respectively. This critical information for these GA signaling genes has the potential to facilitate future genetic engineering and breeding of P. ginseng for increased crop yield and production of useful substances.

• Journal of Animal Science and Technology
• /
• v.63 no.4
• /
• pp.854-863
• /
• 2021

Weaning induces physiological changes in intestinal development that affect pigs' growth performance and susceptibility to disease. As a posttranscriptional regulator, microRNAs (miRNAs) regulate cellular homeostasis during intestinal development. We performed small RNA expression profiling in the small intestine of piglets before weaning (BW), 1 week after weaning (1W), and 2 weeks after weaning (2W) to identify weaning-associated differentially expressed miRNAs. We identified 38 differentially expressed miRNAs with varying expression levels among BW, 1W, and 2W. Then, we classified expression patterns of the identified miRNAs into four types. ssc-miR-196a and ssc-miR-451 represent pattern 1, which had an increased expression at 1W and a decreased expression at 2W. ssc-miR-499-5p represents pattern 2, which had an increased expression at 1W and a stable expression at 2W. ssc-miR-7135-3p and ssc-miR-144 represent pattern 3, which had a stable expression at 1W and a decreased expression at 2W. Eleven miRNAs (ssc-miR-542-3p, ssc-miR-214, ssc-miR-758, ssc-miR-4331, ssc-miR-105-1, ssc-miR-1285, ssc-miR-10a-5p, ssc-miR-4332, ssc-miR-503, ssc-miR-6782-3p, and ssc-miR-424-5p) represent pattern 4, which had a decreased expression at 1W and a stable expression at 2W. Moreover, we identified 133 candidate targets for miR-196a using a target prediction database. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that the target genes were associated with 19 biological processes, 4 cellular components, 8 molecular functions, and 7 KEGG pathways, including anterior/posterior pattern specification as well as the cancer, PI3K-Akt, MAPK, GnRH, and neurotrophin signaling pathways. These findings suggest that miRNAs regulate the development of the small intestine during the weaning process in piglets by anterior/posterior pattern specification as well as the cancer, PI3K-Akt, MAPK, GnRH, and neurotrophin signaling pathways.

• Journal of Breast Disease
• /
• v.6 no.2
• /
• pp.35-38
• /
• 2018

Purpose: The eighth American Joint Committee on Cancer staging system for breast cancer was recently published to more accurately predict the prognosis by adding biomarkers such as estrogen receptors, progesterone receptors, and human epidermal growth factor receptor 2. However, this system is very complicated and difficult to use by clinicians. The authors developed a program to aid in setting up the staging system and confirmed its usefulness by applying it to theoretical combinations and actual clinical data. Methods: The program was developed using the Microsoft Excel Macro. It was used for the anatomic, clinical and pathological prognostic staging of 588 theoretical combinations. The stages were also calculated the stages using 840 patients with breast cancer without carcinoma in situ or distant metastasis who did not undergo preoperative chemotherapy. Results: The anatomic, clinical and pathological prognostic stages were identical in 240 out of 588 theoretical combinations. In the actual patients' data, stages IB and IIIB were more frequent in clinical and pathological prognostic stages than in the anatomic stage. The anatomic stage was similar to the clinical prognostic stage in 58.2% and to the pathological prognostic stage in 61.9% of patients. Oncotype DX changed the pathological prognostic stage in 2.1% of patients. Conclusion: We developed a program for the new American Joint Committee on Cancer staging system that will be useful for clinical prognostic prediction and large survival data analysis.

• Atmosphere
• /
• v.29 no.2
• /
• pp.213-218
• /
• 2019

A valuation of air pollution is critical for relevant policy-making for the public and research direction. This study conducted a willingness-to-pay (WTP) survey using contingent valuation method (CVM) in Seoul Korea. In detail, this study puts an emphasis on its temporal changes referred on two surveys conducted in 2014 and 2019. In reference to the previous studies in 2002, our survey indicated that the public awareness of air pollution and interests on its sources and solutions increased noticeably. Our survey showed that WTP increased significantly from 2588 to 4827 and 8240 Korean Won $month^{-1}$ in 2002, 2014, and 2019, respectively. Moreover, the percentage of respondents to pay the WTP also increased from 48% to 68% and 79% in 2002, 2014, and 2019, respectively. Our analysis based on the number of Google search on particulate matters (PMs) strongly suggests that such the noticeable increases of the public attention to air pollution is well accorded with the moment of the announcement of a standard for ultra-fine particles and the start of PM prediction in late 2013. But the Google search rate grew about 16.7 times in 2009 compared to 2014, which is much larger than the growth rate of interest and WTP between 2014 and 2019. Our results shed light on policy decision for the right person in the right place on the right time in the era of air pollution.

• Journal of Climate Change Research
• /
• v.5 no.2
• /
• pp.109-125
• /
• 2014

Suitable climate condition is essential for stable growth of crops which directly leads to an increase in crop production. Preceding domestic researches mostly used crop models to predict grain or crop yield in relation to climate change. However, the use of various models and input data based on foreign background lowered the reliability for result. Therefore in this study, we evaluated domestic applicability by comparing and analyzing various crop models developed abroad. In addition, we selected models based on the possibility of acquiring input data and suggested domestic applicability.

• Advances in Computational Design
• /
• v.4 no.2
• /
• pp.73-90
• /
• 2019

The growth in the number of single-member households is a critical issue worldwide, especially among the elderly. For those living alone, who may be unaware of their health status or routines that could improve their health, a continuous healthcare monitoring system could provide valuable feedback. Assessing the performance adequacy of activities of daily living (ADL) can serve as a measure of an individual's health status; previous research has focused on determining a person's daily activities and extracting the most frequently performed behavioral patterns using camera recordings or wearable sensing techniques. However, existing methods used to extract common patterns of an occupant's activities in the home fail to address the spatio-temporal dimensions of human activities simultaneously. Though multiple sequence alignment (MSA) offers some advantages - such as inherent containment of the spatio-temporal data in sequence format, and rapid identification of hidden patterns - MSA has rarely been used to extract in-home ADL routines. This research proposes a method to extract a household occupant's ADL routines from a cumulative spatio-temporal data log of occupancy collected using a non-intrusive method (i.e., a tomographic motion detection system). The findings from an occupant's 28-day spatio-temporal activity log demonstrate the capacity of the proposed approach to identify routine patterns of an occupant's daily activities and to reveal the order, duration, and frequency of routine activities. Routine ADL patterns identified from the proposed approach are expected to provide a basis for detecting/evaluating abrupt or gradual changes of an occupant's ADL patterns that result from a physical or mental disorder, and can offer valuable information for home automation applications by enabling the prediction of ADL patterns.

• Asian-Australasian Journal of Animal Sciences
• /
• v.31 no.12
• /
• pp.1980-1990
• /
• 2018

Objective: The aim of this study was to discover the functional impact of non-synonymous single nucleotide polymorphisms (nsSNPs) that were found in selective sweep regions of the Landrace genome Methods: Whole-genome re-sequencing data were obtained from 40 pigs, including 14 Landrace, 16 Yorkshire, and 10 wild boars, which were generated with the Illumina HiSeq 2000 platform. The nsSNPs in the selective sweep regions of the Landrace genome were identified, and the impacts of these variations on protein function were predicted to reveal their potential association with traits of the Landrace breed, such as reproductive capacity. Results: Total of 53,998 nsSNPs in the mapped regions of pigs were identified, and among them, 345 nsSNPs were found in the selective sweep regions of the Landrace genome which were reported previously. The genes featuring these nsSNPs fell into various functional categories, such as reproductive capacity or growth and development during the perinatal period. The impacts of amino acid sequence changes by nsSNPs on protein function were predicted using two in silico SNP prediction algorithms, i.e., sorting intolerant from tolerant and polymorphism phenotyping v2, to reveal their potential roles in biological processes that might be associated with the reproductive capacity of the Landrace breed. Conclusion: The findings elucidated the domestication history of the Landrace breed and illustrated how Landrace domestication led to patterns of genetic variation related to superior reproductive capacity. Our novel findings will help understand the process of Landrace domestication at the genome level and provide SNPs that are informative for breeding.

• Fire Science and Engineering
• /
• v.32 no.6
• /
• pp.55-62
• /
• 2018

To improve the prediction result with enhanced reliability of domestic Performance-Based Design (PBD), actual scale fire tests were carried out on products made of plastics from sales facility combustibles. The commercial buildings were separated into single and multiple combustibles for the experimentation of fire spread caused by the sales shelves where the various combustible materials are displayed. A according to the maximum heat release rate, exposed area and weight of the combustible material, the results revealed a linear relationship of as 93% and 89%. In addition, analysis of the gas concentrations for various combustibles showed that $CO_2$ has a linear relationship, whereas the CO concentration indicated exponential function. These results can be applied to reliable fire source information in PBD of plastic fire source in commercial buildings. This may be applied as fire source information representative of a plastic fire in commercial buildings through additional experiment using the area of the shelf in actual commercial buildings.