• Title/Summary/Keyword: growth pH

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Growth Characteristics of Enterobacter sakazakii Used to Develop a Predictive Model

  • Seo, Kyo-Young;Heo, Sun-Kyung;Bae, Dong-Ho;Oh, Deog-Hwan;Ha, Sang-Do
    • Food Science and Biotechnology
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    • v.17 no.3
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    • pp.642-650
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    • 2008
  • A mathematical model was developed for predicting the growth rate of Enterobacter sakazakii in tryptic soy broth medium as a function of the combined effects of temperature (5, 10, 20, 30, and $40^{\circ}C$), pH (4, 5, 6, 7, 8, 9, and 10), and the NaCl concentration (0, 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10%). With all experimental variables, the primary models showed a good fit ($R^2=0.8965$ to 0.9994) to a modified Gompertz equation to obtain growth rates. The secondary model was 'In specific growth $rate=-0.38116+(0.01281^*Temp)+(0.07993^*pH)+(0.00618^*NaCl)+(-0.00018^*Temp^2)+(-0.00551^*pH^2)+(-0.00093^*NaCl^2)+(0.00013^*Temp*pH)+(-0.00038^*Temp*NaCl)+(-0.00023^*pH^*NaCl)$'. This model is thought to be appropriate for predicting growth rates on the basis of a correlation coefficient (r) 0.9579, a coefficient of determination ($R^2$) 0.91, a mean square error 0.026, a bias factor 1.03, and an accuracy factor 1.13. Our secondary model provided reliable predictions of growth rates for E. sakazakii in broth with the combined effects of temperature, NaCl concentration, and pH.

Growth Optimization of Photorhabdus luminescens Isolated from Entomopathogenic Nematode Heterorhabditis bacteriophora (병원성 선충 Heterorhabditis bacteriophora에서 분리된 공생 박테리아 Photorhabdus luminescens의 생장조건)

  • Yoo, Sun Kyun;Randy Gaugler;Christopher W. Brey
    • Microbiology and Biotechnology Letters
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    • v.29 no.2
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    • pp.104-109
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    • 2001
  • The yield of infective juveniles of Heterorhabditis bacteriophora (Tf strain) in vitro monoxenic liquid culture was improved significantly as the amount of symbiont biomass, Photorhabdus sp. strain Tf, increased. To investigate the influence of abiotic factors on the growth and biomass production of Photorhabdus sp. strain Tf, triplicate flask cu1tmes were performed. The optinal temperature and medium pH for the growth of Photorhahdus sp. strain Tf were 30$^{\circ}$C and between pH 5.5-7.3, respectively. Aeration also improved greatly growth and yield of biomass of Photorhabdus sp. strain Tf. Photorhabdus sp. strain Tf in batch fermentation showed growth-associated pattem in terms of pigment production, and the pH of culture medium rose steadily until growth stopped dUling the fermentation. Both pigment production and culture pH rise would be useful parameters indicating a reliable growth of Photorhabdus sp. strain Tf.

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Effect of Red Ginseng, Extract on Growth of Lactobacillus sp., Escherichia coli and Listeria monocytogenes in pH Controled Medium (배지의 pH 조정에 의한 홍삼 추출물 첨가가 Lactobacillus SP., Escherichia coli와 Listeria monocytogenes의 생육에 미치는 영향)

  • Bae Hyoung-Churl;Lee Jo-Yoon;Nam Myoung Soo
    • Food Science of Animal Resources
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    • v.25 no.2
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    • pp.257-264
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    • 2005
  • This experiment was carried out to investigate the effect of red ginseng extract on the growth of Lactobacillus sp. (L acidophilus, L casei, L salivarius), Escherichia coli and Listeria monocytogenes in pH controled medium by $\beta-Glycerol\;PO_4$ buffer. The growth of Lactobacillus sp. was show a similar pattern in control and MRS broth with red ginseng extract $1.0\%$ but was remarkably show inhibiting in MRS broth with over $2.0\%$ red ginseng extracts. The growth of E coli was inhibited in Trypticase soy broth with $1.0\%$ red ginseng extracts. Also the growth of L monocytogenes was inhibited in Trypticase soy broth with $5.0\%$ red ginseng extract The growth of L acidophilus KCTC3150, L casei KCTC3189, L salivarius ssp. salivarius CNU27, and E coli KCTC1039, L monocytogenes KCTC3443 were remarkably inhibited in pH non-control medium and pH control medium with $10\%$ red ginseng extract These results was suggested to effect of inhibition of microorganisms growth not pH decrease by organic acid but another components in red ginseng extract.

Subcritical crack growth in rocks in an aqueous environment (수성환경에서 암석 내의 임계하 균열성장 연구)

  • Nara, Yoshitaka;Takada, Masafumi;Igarashi, Toshifumi;Hiroyoshi, Naoki;Kaneko, Katsuhiko
    • Geophysics and Geophysical Exploration
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    • v.12 no.1
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    • pp.163-171
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    • 2009
  • Subcritical crack growth is one of the main causes of time-dependent fracturing in rock. In the present study, we investigated subcritical crack growth in rock in distilled water (pH = 5.7) and in an aqueous solution of sodium hydroxide (NaOHaq, pH = 12), comparing the results to those in air. We also investigated the effect of the pH in an aqueous environment. We used andesite and granite for all our tests. We determined the relationship between the crack velocity and the stress intensity factor using the double-torsion test under conditions of controlled temperature. We showed that crack velocities in water were higher than those in air, in agreement with other research results indicating that crack velocity increases in water. When we compared our results for NaOHaq with those for water, however, we found that the crack velocity at the same stress intensity factor did not change even though the pH of the surrounding environment was different. This result does not agree with the accepted understanding that hydroxide ions accelerate subcritical crack growth in rocks. We concluded that the pH at the crack tip influences subcritical crack growth, and not the bulk pH, which has little effect.

Direct Evidence of Intracellular Alkalinization in Saccharomyces cerevisiae KNU5377 Exposed to Inorganic Sulfuric Acid

  • Yun, Hae-Sun;Paik, Sang-Kyoo;Kim, Il-Sup;Jin, Ing-Nyol;Sohn, Ho-Yong
    • Journal of Microbiology and Biotechnology
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    • v.14 no.2
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    • pp.243-249
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    • 2004
  • The toxicity of inorganic sulfuric acid as a stressor was characterized in Saccharomyces cerevisiae KNU5377. In this work, we examined physiological responses to low extracellular pH $(pH_{ex})$ caused by inorganic $H_2SO_4$, which could not affect cell growth after pH was adjusted to an optimum with Trizma base. The major toxicity of sulfuric and was found to be reduction of environmental pH, resulting in stimulation of plasma membrane ${H^+}-ATPase$, which in turn contributed to intracellular alkalinization. Using a pH-dependent fluorescence probe, 5-(and-6)-carboxy SNARF-1, acetoxymethyl ester, acetate (carboxy SNARF-1 AM acetate), to determine $pH_{in}$, we found that color was dependent on the changes of intracellular pH which coincided with calculated $pH_{in}$ of alkalinization up to approximately pH 7.3. This alkalinization did not seem to affect survival of these cells exposed to 30 mM sulfuric acid, which lowered the $pH_{ex}$ of the glucose containing growth media up to approximately pH 3.0; however, the cells could grow only up to 70% of the maximum growth in the same media, when 30 mM sulfuric acid was added.

Performance Research of a Jacket Cooling Water System in a Diesel Electric Generation (디젤발전 자켓냉각시스템 열성능 향상 연구)

  • Lee, Jae-Keun;Moon, Jeon-Soo;Yoon, Seok-Won;Park, Pill-Yang
    • Transactions of the Korean hydrogen and new energy society
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    • v.20 no.6
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    • pp.534-539
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    • 2009
  • One of the most efficient techniques improving the heat transfer performance of a diesel electric generation is a corrosion control in jacket cooling water system. The environmental parameters most affecting corrosion are dissolved salt concentration, temperature, and pH of cooling water. No corrosion occurs in carbon steel probe at pH 11 in normal operating condition of diesel electric generation cooling water. pH control agent in this study is trisodium phosphate. pH control appears to be the most convenient way to enhance the thermal performance of a diesel electric generation.

The Effect of Simulated Acid Rain on the Growth of Important Crops (인공 산성비가 중요 농작물의 생육에 미치는 영향)

  • 허흥욱;허만규
    • Journal of Environmental Science International
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    • v.7 no.2
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    • pp.123-132
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    • 1998
  • The experiment was performed to elucidate the effect of simulated acrid rain on growth of the seven crops(caucus carota vu sativa DC. , Fagopyrum esculenkm Moench, Brassica cmpespis subsp. napus var. pekinensis Makino , Raphuus satlws vu. hortensis for. acnMormis Makino, Brassica dbogjabra Bailey, Caphslcum mum L., and Perilla frutescens Britton). The pH levels of simulated acid rain ranged pH 3.1, 3.6, 4.1, 4.6, 5.1 and 5.6. The germination 10 each crop was influenced from stimulated acrid rain except buckwheat and kale. A general decrease of growth in crops was observed with Increasing pH concentrations. The pattern of soluble protein was observed a tendency to decrease from acidic pH eradlents. According to acidity, total DNA contents of each crop was showed a definite reduction. In conclusion, plant growth was stimulated decreasln and the chanties of total Protein Patterns and BNA contents extracted from leaves trended with stimulated acrid rain was showed seriously.

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Processing Conditions of Low-Salt Fermented Squid and Its Flavor Components 2. Effects of Temperature, Salinity and pH on the Growth of Bacteria from Isolated Low Salt Fermented Squid (저염 오징어젓갈 제조 방법 및 향미 성분 2. 온도, 염도 및 pH가 저염 오징어젓갈 숙성 세균의 발육에 미치는 영향)

  • 김영만;이원재;정윤미;허성호;최성희
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.4
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    • pp.631-635
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    • 1995
  • In order to develop effective manufacturing method and to improve quality of low-salt fermented squid(10% of table salt), we investigated the effects of temperature, salinity and pH on the growth of Staphylococcus xylosus, Micrococcus varians, Pseudomonas diminuta and Pseudomonas D2 isolated from of low-salt fermented squid and the growth characteristics of these bacteria during fermentation were elucidated. All bacteria showed good growth during the process of low-salt fermented squid(pH 6~7 ; concentration of NaCl, 7~10% ; temperature, 7~1$0^{\circ}C$) and their cell numbers increased as fermentation proceeded under the same fermentation condition.

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Quantitative analysis of Spirulina platensis growth with CO2 mixed aeration

  • Kim, Yong Sang;Lee, Sang-Hun
    • Environmental Engineering Research
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    • v.23 no.2
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    • pp.216-222
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    • 2018
  • The growth characteristics of Spirulina platensis were investigated using four photo-bioreactors with $CO_2$-mixed air flows. Each reactor was operated under a specific condition: 3% $CO_2$ at 50 mL/min, 3% $CO_2$ at 150 mL/min, 6% $CO_2$ at 50 mL/min, and 6% CO2 at 150 mL/min. The 3% $CO_2$ at 150 mL/min condition produced the highest algal growth rate, while the 6% $CO_2$ at 150 mL/min conditioned produced the lowest. The algal growth performance was suitably assessed by the linear growth curve rather than the exponential growth. The medium pH decreased from 9.5 to 8.7-8.8 (3% $CO_2$) and 8.4-8.5 (6% $CO_2$), of which trends were predicted only by the pH-carbonate equilibrium and the reaction kinetics between dissolved $CO_2$ and $HCO_3{^-}$. Based on the stoichiometry between the nutrient amounts and cell elements, it was predicted that depleted nitrogen (N) at the early stage of the cultivation would reduce the algal growth rates due to nutrient starvation. In this study, use of the photobioreactors capable of good light energy distribution, proper ranges of $CO_2$ in bubbles and medium pH facilitated production of high amounts of algal biomass despite N limitation.

Growth Response to Acid Rain, Mg Deficiency and Al Surplus, and Amelioration of Al Toxicity by Humic Substances in Pitch Pine Seedlings

  • Joon-Ho Kim
    • Journal of Plant Biology
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    • v.37 no.3
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    • pp.301-308
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    • 1994
  • The individual and combined effects of acidic rain, Mg deficiency (-Mg) and Al surplus (+Al) on the growth of shoots and roots of pitch pine seedlings and the effect of humic substances (Lit) on Al toxicity were investigated. The growth of height and dry matter were not significantly less for pitch pine seedlings sprayed with simulated acid rain (SAR) of pH 3.5 than for those sprayed with SAR of pH 5.6. But treatments of Al and +Al-Mg in soil solution reduced the growth of seedlings in terms of height of shoots, and dry matter of shoots or roots. Effect of Mg deficiency on the growth of seedlings was apparent only when Al was treated simutaneously. The growth of seedlings, regardless of rain pH, decreased in the following order: control=-Mg>Lit+Al>+Al>+Al-Mg. Treatments of Al and +Al-Mg in soil solution reduced the total length of secondary and teritary roots of seedlings regardless of rain pH, and decreased in the following order: the primary root

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