• Korean Journal of Mineralogy and Petrology
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• v.36 no.4
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• pp.303-312
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• 2023

The determination of the oxidation states of metal elements in manganese nodules sheds light on the understanding of the formation mechanism of nodules, providing insights into the paleo-environmental conditions such as the redox potential of the aqueous system. This study aims to reveal the oxidation states and chemical bonding of manganese in the natural polymetallic nodules, utilizing conventional X-ray photoelectron spectroscopy (XPS). Specifically, shallow manganese nodules from the Siberian Arctic Sea, effectively recording mineralogical variations, were used in this study. Detailed analysis of XPS Mn 2p spectra showed changes in the manganese oxidation state from the center to the outer parts of the nodules. The central part of the nodules showed a higher Mn⁴⁺ content, approximately 67.9%, while the outermost part showed about 63% of Mn⁴⁺ due to an increase in the Mn³⁺+Mn²⁺. The decrease in the Mn oxidation state with the growth is consistent with the previously reported mineralogical variations from todorokite to birnessite with growth. Additionally, the O 1s spectra presented a predominance of Mn-O-H bonds in the outer layers compared to the center, suggesting hydration by water in the layered manganates of outer layers. The results of this study demonstrate that XPS can be directly applied to understand changes in paleo-environmental conditions such as the redox states during the growth of manganese nodules. Finally, future studies using high-resolution synchrotron-based XPS experiments could achieve details in oxidation states of manganese and trace metal elements.

• Journal of Life Science
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• v.34 no.5
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• pp.304-312
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• 2024

This study was conducted to characterize strain Y10, isolated from discarded chicken feathers. Strain Y10 was identified as Bacillus amyloliquefaciens through phenotypic and 16S rRNA gene analysis. B. amyloliquefaciens Y10 exhibited plant growth-promoting activities, including the production of fungal cell-degrading enzymes (cellulase, lipase, protease, and pectinase), siderophores, ammonia, and indoleacetic acid. Furthermore, strain Y10 was able to inhibit the mycelial growth of several phytopathogenic fungi. When 0.1% sucrose as a carbon source and 0.05% casein as a nitrogen source were added to the basal medium, adjusted to pH 10, and cultured at 35℃, the degradation rate of chicken feathers by strain Y10 was about two times higher than that of the basal medium, with the feathers almost completely degraded in four days. Strain Y10 also degraded various keratin substrates, including duck feathers, wool, and human nails. It was confirmed that the feather hydrolyzates prepared using strain Y10 exhibited antioxidant activities, such as 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity (EC₅₀ = 0.38 mg/ml) and superoxide dismutase-like activity (EC₅₀ = 183.7 mg/ml). These results suggest that B. amyloliquefaciens Y10 is a potential candidate for the development of bioinoculants and feed additives applicable to the agricultural and livestock industries, as well as the microbiological treatment of keratin waste.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.6 no.4
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• pp.62-73
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• 2003

The study was to apply a mat style reed planting method at confluence to improve plants growth conditions by relaxing disturbed topographical base due to water flow and was intended to review the effect to build up a vegetation strip by monitoring process after the construction. First off, We've attempted to construct reed mats on selected sites as confluences of Tan and Gaehwa stream and then examined and analyzed characteristics of soil and vegetation community. As the results of the examination, the soil texture was proven to be a mix of sand and loamy sand and be 6.3 ~ 7.0 soil pH. In addition, it contained 1.0 ~ 4.6% of organic matter, 0.04 ~ 0.22% of T-N and 27.8 ~ 41.2% of water content. For its vegetation structure, the Tan stream confluence was first actually a point bar without plants prior to the construction but 8 kinds of hygrophytes including Persicaria hydropiper and 9 kinds of terrestrial plants such as Potentilla supina, Artemisia annua, and Alopecurus aequalis var. amurensis. On the other hand, the Gaehwa stream confluence contained 6 kinds of hygrophytes such as Bidens frondosa and other 11 kinds of terrestrial plants prior to the construction while it produced 7 kinds of hygrophytes including Ranunculus ternatus as well as Phragmites australis and 9 kinds of terrestrial plants such as Potentilla supina after the construction. For the Phragmites australis, almost of them was weathered away in early days just after planting because of development period passed, but on May, six months later from planting, it was investigated that its length was approximated as 65 ~ 85cm with 75% coverage and that the number of it was 437 ~ 633/$m^2$. The study was shown that reed mats can improve environmental conditions of disturbed topographical base, enabling natural growth of various riparian vegetation including the introduced plant, reed. In the meantime, it was supposedly judged that to recover or build up a vegetarian strip, supplementary materials should be prepared to help produce and grow plants because it is not probable to expect river drift by water flow at confluence and that corrosion, burying or inundation owing to changes of water lever should be considered.

• Journal of Food Hygiene and Safety
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• v.28 no.3
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• pp.241-246
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• 2013

The objective of this study was to evaluate microbial growth patterns on Korean foods containing collagen. Samgyetang, Ugultang, Odolbyeboguem, Jeonyak, and Jokbal were inoculated with Staphylococcus aureus and Salmonella spp., and incubated at $4^{\circ}C$ (6 days), $10^{\circ}C$ (6 days), $20^{\circ}C$ (3 days), and $30^{\circ}C$ (36 h). Cell counts of S. aureus and Salmonella spp. were enumerated on mannitol salt agar and xylose lysine deoxycholate agar, respectively. Of the 5 foods, S. aureus and Salmonella cell counts decreased (P < 0.05) only in Jeonyak. To understand this reduction in cell growth on Jeonyak, extracts of ingredients such as clove, cinnamon, pepper, ginger, and jujube were examined to determine minimal bactericidal concentrations (MBC; AU/mL). Clove (3.13 AU/mL), cinnamon (50 AU/mL), pepper (25 AU/mL), and ginger (50 AU/mL) extracts displayed antimicrobial activity against S. aureus, and clove and cinnamon extracts displayed antimicrobial activity against Salmonella. The results indicate that Jeonyak has antimicrobial activity against S. aureus and Salmonella, and clove and cinnamon are the primary antimicrobial agents in this food.

• The Korean Journal of Mycology
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• v.15 no.1
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• pp.42-47
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• 1987

4 strains of the oak mushroom Lentinus edodes including the strain ASI 3046 were collected home and abroad to investigate the characterics of mycelial growth on synthetic media and sawdust media. The possibility of artificial culture of Lentinus edodes on media composed of different saw dusts and adding materials was investigated. Results were as follows: The optimum temperature for growth ranged from $22^{\circ}C$ to $26^{\circ}C$ among the lineages of Lentinus edodes. The optimal growth temperature for ASI 3046 strain and ASI 3047 strain was $26^{\circ}C$ and $22^{\circ}C$, respectively. The optimal carbon source for ASI 3046 strain was glucose, and peptone and ammoinum tartrate were proved excellent as nitrogen sources. When Lentinus edodes was cultured on sawdust media by varying the mixing ratio of different sawdusts and adding materials, mixed sawdust media composed of oak sawdust(50%, v/v), poplar sawdust(50%, v/v), acorn powder(5%, v/v) and rice bran(10%, v/v) showed higher yields of fruit bodies. Results showed that the ideal strain of Lentinus edodes for artificial culture on sawdust media was strain ASI 3046.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.12 no.3
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• pp.161-166
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• 1979

To know the influence of temperature on the fermentation process, a strain of Lactobacillus bulgarius was experimentally cultured three different temperature conditions of $39^{\circ}C,\;42^{\circ}C\;and\;45^{\circ}C$, pH 5.8 and mechanical agitation of 500rpm. During 20 hour's fermentation, the microbial growth attained the maximum concentration under the conditions mentioned above. However, the culturing conditions resulted different outcomes in terms of maximum concentration of the microbes and the residual concentration of substrate. Among the three temperature conditions, the fermentation at $45^{\circ}C$ was most effective and the maximum specific growth temperature conditions, the fermentation at $45^{\circ}C$ was most effective and the maximum specific growth rate was 0.58/hr. Activation energy deduced from the Arrhenius equation was 9,220cal/mole and entropy was $-33.74\;cal/^{\circ}K$ mole. Activation enthalpy was 9,845 cal/mole and free energy was 19,800 cal/mole.

• Journal of the Korean Institute of Landscape Architecture
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• v.32 no.6 s.107
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• pp.103-108
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• 2005

It was aimed to promote as a material for interior landscape by validating Rumohra aristata, in an indoor environment, especially irrigation interval, medium composition and drainage at indoor. 1. The result of physico-chemical analysis of medium composition showed that porosity, pH and Ex-Ca, Ex-Mg and Ex-K were high with peatmoss: vermiculite: perlite(1:1:1) and water contents, organic matter content, total nitrogen and cation exchange capacity were high with sand: leaf mold(1:1). 2. Growth and indoor adaptability of Rumohra aristata were better with irrigation at 2 interval per week than irrigation at 7 interval per week regardless of drainage. 3. In the case of medium composition, of growth and indoor adaptability were higher with sud: leaf mold (1:1) than peatmoss: vermiculite: perlite(1:1:1). 4. Fronds fresh weight and dry weight decreased when irrigation interval increased and were higher with sand: leaf mold(1:1) than peatmoss: vermiculite: perlite(1:1:1) treatment.

• KSBB Journal
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• v.14 no.6
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• pp.718-723
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• 1999

In order to treat of alkaline dye-processing wastewater, alkalophilic strains biodegrading azo dye, Acid red 1, is isolated from natural system, and optimal culture conditions are examined using response surface analysis, statistical analysis system program. 15 different species which grow in alkaline culture media are isolated from the effluent and river soil discharged from wastewater treatment plant in dye industrial complex. One strain which has the best decolorization efficiency is chosen, and named as AR-1. The result of the examination of carbon, nitrogen and phosphorus sources which have influence on growth and decolorization reveals that optimum carbon, nitrogen and phosphorus sources are 1.0% fructose, 1.0% polypeptone, 1.0% yeast extract and 0.5% $K_2HPO_4$, respectively. In order to optimize of biodegradation conditions of dye by response surface analysis, the characteristics of decolorization and cell growth according to culture temperature and time are monitered. The result shows that the one is optimum 34.77$^{\circ}C$ for 12.97 hours; the other at 34.73$^{\circ}C$ for 12.96 hours. While, optimal conditions of culture that satisfy both cell growth and decolorization are the temperatures from 32.86$^{\circ}C$ to 36.36$^{\circ}C$ and the period of 10.96 to 15.75 hours, respectively.

• Journal of Periodontal and Implant Science
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• v.26 no.4
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• pp.889-905
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• 1996

It is known that growth factors function as potent biologic mediators regulating numerous activities of wound healing via cell proliferation, migration and extracellular matrix formation and they also promote periodontal regeneration. But, method of growth factor application is controversial yet. So purpose of this study is to evaluate the effect of demineralized root surface as one of method of growth factor application. The ginigival fibroblasts were primary cultured and fifth or sixth subpassages were used in these experiments. In first experiment, root surface blocks demineralized with 100mg/ml tetracycline for 5 minutes and pH 1 citric acid for 3 minutes(experimental groups) and nonteminerilized root surface blocks (control groups) were placed in 100ng/ml PDGF-BB for 5 minutes. Then the cells were seeded on each root surface blocks and cultured for 6, 24, 48, 72 hours. In second experiment, root surface blocks deminerilized with tetracycline and citric acid and nondemineralized root surface blocks were placed in 200ng/ml PDGF-BB for 5 minutes and another non-demineralized root surfcae blocks were placed in DMEM without PDGF-BB. At 1, 2, 4, 6, 8 days, the cells were seeded in 24-well plate and using of each eluent, cultured for 72 hours. The results of the four determinants were presented as mean and S.D.. The results were as follows : The attachment and proliferation of human gingival fibroblast on root surface were more increased when PDGF-BB was applicated on root surfrace demineralized with tetracycline or citric acid than non-demineralized root surface. And, in comparision tetracycline with citric acid, there were more attachment and proliferation of human gingival fibroblast on root surface demineralized with tetracycline than citric acid, and proliferation of human gingival fibroblast on demineralized root surface was increased time dependently 1 day to 3 days. In second experiment using eluent, proliferation of human gingival fibroblast was more increased to 6 days when human gingival fibroblast was cultured in eluent that PDGF-BB was applicated on demineralized root surface than two control groups, and degree of proliferation was decreased time dependently 1 day to 6 days. Proliferation of human gingival fibroblast cultured in eluent without PDGF-BB was constant 1 day to 6 days. After 6 days, degree of proliferation of human gingival fibroblast was similar in four groups. This means that release duration of PDGF-BB from demineralized root surface is 6 days. And in comparision tetracycline with citric acid, there was more proliferation of human gingival fibroblast in tetracycline-treated group than citric acid. In conclusion, demineralized root surface as primary site for PDGF-BB application, especially demineralized with tetracycline has important roles in attachment and proliferation of human gingival fibroblast, and may be useful clinical applications in periodontal regenerative procedures.

• Molecular & Cellular Toxicology
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• v.1 no.2
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• pp.116-123
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• 2005

The application of high pressure on cellular morphology, proliferation and protein expression of Jurkat cells (human T lymphocyte cell line) has been extensively investigated. In the present study, we manufactured a novel pressure chamber that modulates 5% $CO_{2}$, temperature and pressure (up to 3 ATA). Jurkat cells was incubated 2 ATA pressure and analyzed cellular morphology and growth using an electron microscopy and MTT assay. The cells showed the morphological changes in the cell surface, which appeared to cause a severe damage in cell membrane. The growth rate of the cells under 2 ATA pressure decreased as cultured time got increased. Furthermore, a long term exposure of high pressure on Jurkat cells may act as one of the important cellular stresses that leads to inducing cell death. Cellular proteomes were separated by 2-dimensional electrophoresis with pH 3-10 ranges of IPG Dry strips. And many proteins showed significant up-and-down expressions with hyperbaric pressure. Out of all, 10 spots were identified significantly using matrix-assisted laser desorption/ionization-time of fight (MALDI-TOF) mass spectrometry. We and found that 9 protein expressions were decreased and one protein, heat shock protein HSP 60, was increased in Jurkat cells under 2 ATA. Identified proteins were related to lipid metabolism and signal transduction.