• 대한치과보철학회지
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• 제31권1호
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• pp.28-38
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• 1993

For the purpose of evaluating the cleansing efficiency against Candida albicans detected frequently in patients with denture stomatitis, two denture cleansers with or without enzymes were studied under the same conditions. The results were as fellows: 1. Enzyme-contain denture cleanser was showed more Candida albicans lytic ability than non-enzyme-contained denture cleanser. 2. It was observed that Candida albicans lytic activity in further diluted manufacturerers' recommended concentration was decreased. 3. In fungicidal test, the enzyme-contained denture cleanser sterilized Candida albicans, and the non-enzyme-contained denture cleanser did not sterilize Candida albicans. 4. Sterilizing time of Candid albicans was needed for at least 60 minutes in enzyme-contained denture cleanser solution which was diluted with manufacturerers' recommended concentrations., and was needed for more times with further diluted manufacturerers' recommended concentrations. 5. In vitro growth test of Candida albicans on acrylic resin surface, the only enzyme-contained denture cleanser inhibited growth of Candida albicans, and it was observed that inhibiton ability of growth of Candida albicans on arrylic resin surface was decreased in further diluted manufacturerers' recommended concentrations.

• The Korean Journal of Ecology
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• 제21권3호
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• pp.179-186
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• 1998

Effects of lead(Pb) and calcium(Ca) on growth responses, accumulation of heavy metals and nitrogen fixation activities of Melilotus suaveolens seedlings were quantitatively analyzed during growing period. Pb contents of the root treated with 30, 100 ppm Pb and 100 ppm Pb added 100 ppm Ca were 54.1, 90.9 and 26.1 folds higher than that of the control, respectively, at pH 4.2 in 28th days, and heavy metal content of plant increased with increasing of pH and Pb concentration. The melilot plant was classified as a Pb accumulator by higher accumulation of Pb in shoot than that of root. Pb treatments resulted in inhibiton of height and chlorophyll contet, and Ca treatment increased height and chlorophyll content insignificantly at pH 4.2 in 28 days. The plant biomass reduced 49, 60 and 54% at pH 4.2 and 47,53 and 50% at pH 6.5, respectively, by 30, 100 ppm Pb and 100 ppm Pb added 100 ppm Ca treatment. Specific nitrogen fixation of nodules reduced 68.4% and 46.6% by 100 ppm Pb treatment and 3.7% and 24.9% by 100 ppm Pb added 100 ppm Ca at pH 4.2 and pH 6.5, respectively, so Ca inhibited significantly Pb activity and toxicity in acdic pH. Nodule formation were reduced to 33, 33 and 50% at pH 4.2 and 50, 33 and 38% at pH 6.5 by 30, 100 ppm Pb and 100 ppm Pb added 100 ppm Ca, respectively.

• Journal of Ginseng Research
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• 제14권2호
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• pp.253-264
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• 1990

The growth responses of a variety of human intestinal bacteria to extracts of Panax ginseng and five other oriental medicinal Araliaceae were elraluattd in vitro and in vivo. The extracts enhanced the growth of Brifidobnnerilrm breve and B. longlim in media with or without carbon sources, suggesting that bifidus factors) might be involved in the phenomenon. This effect was most pronounced with water extract of P. ginseng, the growth of 27 bifidobacteria strains belonging to B adolescentis, B. longum, B. brim and B. infantis being greatly stimurated, whereas seven B. bifidum strains and other bacteria such as clostridia and Escherichin soli had little or no ability to utilise it for growth. Methanol extracts of p. ginseng were found to selectively inhibit growth of various clostridia including bifidobacteria. Paraputrificum, but this effect was not observed on other bacteria including bifidobacteria. The effect of ginseng extract intake (600 mg/day for two weeks) on the faecal microflora, pH, volatile fatty acids, ammonia, putrefactive products, and -glucuronidase, -glucosidase and nitroreductase activities, and on the blood components (triglyceride, total cholesterol and ammonia) were investigated using seven healthy human volunteers. The total concentration of faecal microflora including Bifidnkaderiifm app. during the period of ginseng extract intake %twas significantly unaffected from the preceding and subsequent control peroids. However, the frequency of occurrence of subjects having C. perfringens was significantly decreased. The faecal pH value was also significantly decreased, suggesting that the intake might increase the activity of Bifidobncterium spry. Other biochemical properties in faeces did not changed significantly. The levels of ammonia and triglycerid in blood were decreased with ginseng extract intake. These results may be an indication of at least one of the Pharmacological actions of p. ginseng as an adaptogen.

• 한국식품영양과학회지
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• 제17권3호
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• pp.215-219
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• 1988

신선돈육의 등심부위를 절단하여 2% 초산용액을 분무하고 $4^{\circ}C$의 냉장고에 보관하면서 무처리구와 경시적으로 미생물학적, 이화학적, 육색의 변화등을 검토하여 저장성 증대의 가능성을 검토하였다. 초산용액의 분무는 신선육표면에 존재하는 aerobic bacteria 및 coliform bactereia의 생육억제에 상당한 효과가 있었다. pH, 단백질변패도(YBN), 지방산패도(TBA)의 측정치는 초산처리구가 대체로 낮게 나와 초산용액이 돈육의 부패를 지연시킬수 없음을 나타내었으며 이들의 값은 미생물의 생육변화와 유사한 양상을 나타내었다. 육색의 변화는 초산용액처리 직후에는 무처리구 보다 검게 나왔으나 저장 2일째 이후부터는 초산용액 처리구가 선홍색쪽에 가까왔다.

• 동의생리병리학회지
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• 제20권3호
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• pp.690-696
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• 2006

Houttuynia cordata Thunb, well known as 'E-Sung-Cho' in Korea, is a traditional medicinal plant generally used in Oriental medicine therapy. In the present study, we investigated the effect of water extract of H. cordafa (WEHC) on the growth of human breast carcinoma MCF-7 cells. Exposure of MCF-7 cells to WEHC resulted in growth inhibition and apoptosis in a dose-dependent manner as measured by MTT assay and fluorescent microscope. The anti-proliferative effect of WEHC was associated with a dose-dependent up-regulation of cyclin-dependent kinase inhibitor p21 in a p53-independent fashion. We found WEHC decreased the levels of cyclooxygenase (COX)-2 and inducible nitric oxide synthease (iNOS) expression without significant changes in the expression of COX-1 , which was correlated with a decrease in prostaglandin $E_2{\;}(PGE_2)$ synthesis. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of H. cordata.

• 생물환경조절학회지
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• 제21권2호
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• pp.140-146
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• 2012

나노 크기인 5nm 이하로 제조한 은나노제품(파이투패치)은 주요 식물병원균인 탄저병원균(Collectotrichum gloeosporioides), 잿빛곰팡이병원균(Botrytis cinerea), 균핵병원균(Sclerotinia sclerotiorum)에 대해 포자발아 및 균사생장을 억제하는 항균력이 있었다. 파이투패치 살포에 의한 고추탄저병 방제효과를 실험하기 위해 파이투패치 희석액에 포자를 침지하여 포습시킨 후 발아율을 조사한 결과 5ppm까지 희석한 처리구에서 병원균의 포자발아억제효과를 보였으며, 균사는 10ppm에서 생장억제효과가 15일간 지속되었다. 특히 파이투패치를 10ppm으로 희석하여 배지표면에 도말한 후 탄저병원균 포자를 접종하면 3일간 발아가 억제되어 식물체 감염을 효과적으로 예방하였으며, 40% 이상 발병한 시험구에 4ppm 파이투패치를 살포한 결과 21일 후 7% 이하의 발병과율로 무처리 대비 70% 방제효과가 있었다. 장마철 탄저병 발생율이 94.6%인 시험포장에서 10ppm 농도로 파이투패치를 7일간격으로 엽면살포한 결과 발병과 발생율이 5.8%로 방제효과를 확인하였으며, 수확한 홍고추를 자연건조한 후에도 발병과율이 24.2%로 건고추 수확량도 증가하였다. 장마철 고추역병(Phytophthora capsici)은 장마가 끝난 무피복시험구에서 8월 11일 15%이었으며 고온기를 지난 9월 7일에는 발병율이 74%로 수확을 포기하였으나, 파이투패치를 코팅처리한 피복재를 씌운 시험구에서는 발병주가 2.3%로 장마철 역병발생이 효과적으로 방제되었다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권15호
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• pp.6549-6556
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• 2015

The PI3K-Akt-mTOR, $Wnt/{\beta}$-catenin and apoptosis signaling pathways have been shown to be involved in genesis of colorectal cancer (CRC). The aim of this study was to elucidate whether combination of Gelam honey and ginger might have chemopreventive properties in HT29 colon cancer cells by modulating the mTOR, $Wnt/{\beta}$-catenin and apoptosis signaling pathways. Treatment with Gelam honey and ginger reduced the viability of the HT29 cells dose dependently with $IC_{50}$ values of 88 mg/ml and 2.15 mg/ml respectively, their while the combined treatment of 2 mg/ml of ginger with 31 mg/ml of Gelam honey inhibited growth of most HT29 cells. Gelam honey, ginger and combination induced apoptosis in a dose dependent manner with the combined treatment exhibiting the highest apoptosis rate. The combined treatment downregulated the gene expressions of Akt, mTOR, Raptor, Rictor, ${\beta}$-catenin, $Gsk3{\beta}$, Tcf4 and cyclin D1 while cytochrome C and caspase 3 genes were shown to be upregulated. In conclusion, the combination of Gelam honey and ginger may serve as a potential therapy in the treatment of colorectal cancer through inhibiton of mTOR, $Wnt/{\beta}$ catenin signaling pathways and induction of apoptosis pathway.

• Journal of Microbiology and Biotechnology
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• 제12권5호
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• pp.858-863
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• 2002

An antimicrobial compound was isolated from the MeOH extract of Catalpa ovata G.$D_{ON}$ fruits, and its structure was Identified as 4,9-dihydroxy-2,2-dimethyl-3,4-Uihydronaphtho[2,3-b]pyran-5,10-dione (HMNP). The antimicrobial activity of the Un was determined by measuring the dose-response inhibiton of microbial growth in liquid cultures and then compared with that of lapachol, a well known antimicrobial 1,4-naphthoquinone. The antimicrobial activity of the HMNP was more effective than that of lapachol over a wide range of test organisms. Gram-positive bacteria, yeast, and fungi ($IC_{50}$ $20-75\muM$) were found to be more sensitive to the HMNP than Cram-negative bacteria ($IC_{50}$ > $100\muM$). The HMNP also inhibited germination of spores of many fungi. The morphological defurmation of the fungal spores was induced by the treatment of HMNP, as illustrated by Scanning Electron Microscopy (SEM).

• 전기화학회지
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• 제25권2호
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• pp.81-87
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• 2022

본 연구에서는, 알킬아민이 구리전해도금에 미치는 영향을 cyclic voltammetry를 이용해 분석해보았다. 수용액상 용해도를 갖는 알킬아민을 도금액에 첨가할 경우, Cu²⁺의 환원반응이 억제되는 것을 확인할 수 있었다. 다양한 알킬아민 중 1,12-diaminododecane에 대해 다양한 농도 및 도금액 조건에서 억제 효과를 관찰하였다. 1,12-diaminododecane은 산성 도금액상에서 protonation 되어, Cu²⁺의 착화제로써 작용하지 않았으며, 따라서 1,12-diaminododecane의 억제 효과는 Cu 표면상 흡착에 의한 것임을 확인할 수 있었다. 1,12-diaminododecane는 (i) protonation에 의한 양이온화와 그에 따른 Cu 표면상 기흡착한 음이온과의 정전기적 인력에 의한 흡착과 (ii) amine에 의한 Cu 표면상 직접 흡착의 두가지 특성을 모두 가지고 있었다. 흡착한 1,12-diaminododecane은 도금 반응을 억제할 뿐만 아니라, 구리도금막 형성시 3차원적 성장과 표면 미세화를 야기하였다.

• 한국약용작물학회지
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• 제12권4호
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• pp.304-308
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• 2004

St. John's Wort의 정상세포에 대한 세포독성을 측정한 결과 두 종류의 추출물 모두 1.0 mg/ml의 높은 농도에서도 정상세포 생존율을 80% 이상으로 유지시켜 정상세포의 안전성이 유지되었다. St. John s wort 추출물은 0.5 mg/ml의 농도에서 간암세포주인 Hep3B에 대하여 80% 이상의 높은 억제율을 나타내었으며, 위암세포주인 AGS에 대하여서는 75% 이상의 높은 생육억제율을 나타내었고, 유방암세포주인 MCF-7에 대하여서는 증류수 추출물들은 0.5 mg/ml 이상의 농도에서 약 80%정도, 에탄올 추출물들은 90% 이상의 매우 높은 암세포 생육억제활성을 나타내었다. 또한 각 추출물의 정상세포독성에 대한 암세포의 억제율을 나타내는 selectivity에 있어서 각 추출물이 4이상의 수치를 나타내어, 암세포를 선택적으로 사멸하는 기작을 지닌 것을 확인하였다. 고추나물의 뿌리 물 추출물들은 인간 B와 T 세포주의 생육을 0.5 mg/ml 이상의 농도에서 T세포의 생장을 2배 이상, B-cell의 경우 $1.2{\sim}1.5$배 이상의 생육 증가를 나타내었으며, 물 추출물들이 에탄올 추출물에 비하여 높은 면역세포의 생육 증진활성을 나타내었다. 배양기간에 따른 면역세포의 생육과 면역물질인 cytokine의 생산을 보면 배양 5일째 두 종류 추출물에서 가장 높은 생존율을 나타내었으며 추출물을 첨가하지 않은 경우에 비교하여 약 1.5배의 생장율을 나타내었고, B 세포에서 IL-6은 에탄올 추출물이 배양 5일째 0.5 mg/ml의 농도에서 최고 6.9 pg/cell의 분비량을 나타내었고, T 세포의 $TNF-{\alpha}$도 에탄올 추출물이 배양 6일째 최고 6.8 pg/cell의 분비량을 나타내었다.